RGB image-based method for phenotyping rust disease progress in pea leaves using R
Rust is a damaging disease affecting vital crops, including pea, and identifying highly resistant genotypes remains a challenge. Accurate measurement of infection levels in large germplasm collections is cruci... (Source: Plant Methods)
Source: Plant Methods - August 21, 2023 Category: Biology Authors: Salvador Osuna-Caballero, Tiago Olivoto, Manuel A. Jim énez-Vaquero, Diego Rubiales and Nicolas Rispail Tags: Methodology Source Type: research
Feature enhancement guided network for yield estimation of high-density jujube
Automatic and precise jujube yield prediction is important for the management of orchards and the allocation of resources. Traditional yield prediction techniques are based on object detection, which predicts ... (Source: Plant Methods)
Source: Plant Methods - August 16, 2023 Category: Biology Authors: Fengna Cheng, Juntao Wei, Shengqin Jiang, Qing Chen, Yu Ru and Hongping Zhou Tags: Methodology Source Type: research
A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments
Tar spot of corn is a significant and spreading disease in the continental U.S. and Canada caused by the obligate biotrophic fungus Phyllachora maydis. As of 2023, tar spot had been reported in 18 U.S. states and... (Source: Plant Methods)
Source: Plant Methods - August 11, 2023 Category: Biology Authors: Jos é E. Solórzano, Shea E. Issendorf, Milton T. Drott, Jill C. Check, Emily M. Roggenkamp, C. D. Cruz, Nathan M. Kleczewski, Carlos C. Gongóra-Canul and Dean K. Malvick Tags: Methodology Source Type: research
DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications
High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of... (Source: Plant Methods)
Source: Plant Methods - August 11, 2023 Category: Biology Authors: Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal and Khaled M. A. Amiri Tags: Methodology Source Type: research
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network
Pumpkin seeds are major oil crops with high nutritional value and high oil content. The collection and identification of different pumpkin germplasm resources play a significant role in the realization of prec... (Source: Plant Methods)
Source: Plant Methods - August 10, 2023 Category: Biology Authors: Xiyao Li, Xuping Feng, Hui Fang, Ningyuan Yang, Guofeng Yang, Zeyu Yu, Jia Shen, Wei Geng and Yong He Tags: Research Source Type: research
Targeted mRNA demethylation in Arabidopsis using plant m6A editor
N6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficu... (Source: Plant Methods)
Source: Plant Methods - August 9, 2023 Category: Biology Authors: Ruiqiu Fang, Xiaolong Chen, Jie Shen and Bin Wang Tags: Research Source Type: research
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH
Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of pr... (Source: Plant Methods)
Source: Plant Methods - August 8, 2023 Category: Biology Authors: Di ána Makai, Edit Mihók, Dávid Polgári, András Cseh, Andrea Lenykó-Thegze, Adél Sepsi and László Sági Tags: Methodology Source Type: research
Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings
Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with... (Source: Plant Methods)
Source: Plant Methods - August 7, 2023 Category: Biology Authors: Yu Hyun Moon, Ui Jeong Woo, Ha Seon Sim, Tae Yeon Lee, Ha Rang Shin, Jung Su Jo and Sung Kyeom Kim Tags: Research Source Type: research
Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Plant virus vectors designed for virus-mediated protein overexpression (VOX), virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost-effective tools for functional genomics studi... (Source: Plant Methods)
Source: Plant Methods - August 3, 2023 Category: Biology Authors: Decai Tuo, Yuan Yao, Pu Yan, Xin Chen, Feihong Qu, Weiqian Xue, Jinping Liu, Hua Kong, Jianchun Guo, Hongguang Cui, Zhaoji Dai and Wentao Shen Tags: Methodology Source Type: research
Three-dimensional branch segmentation and phenotype extraction of maize tassel based on deep learning
The morphological structure phenotype of maize tassel plays an important role in plant growth, reproduction, and yield formation. It is an important step in the distinctness, uniformity, and stability (DUS) te... (Source: Plant Methods)
Source: Plant Methods - August 1, 2023 Category: Biology Authors: Wenqi Zhang, Sheng Wu, Weiliang Wen, Xianju Lu, Chuanyu Wang, Wenbo Gou, Yuankun Li, Xinyu Guo and Chunjiang Zhao Tags: Research Source Type: research
Segmentation and counting of wheat spike grains based on deep learning and textural feature
This study developed an advanced deep lea... (Source: Plant Methods)
Source: Plant Methods - August 1, 2023 Category: Biology Authors: Xin Xu, Qing Geng, Feng Gao, Du Xiong, Hongbo Qiao and Xinming Ma Tags: Methodology Source Type: research
Atmospheric correction of vegetation reflectance with simulation-trained deep learning for ground-based hyperspectral remote sensing
Vegetation spectral reflectance obtained with hyperspectral imaging (HSI) offer non-invasive means for the non-destructive study of their physiological status. The light intensity at visible and near-infrared ... (Source: Plant Methods)
Source: Plant Methods - July 29, 2023 Category: Biology Authors: Farid Qamar and Gregory Dobler Tags: Methodology Source Type: research
Intelligent identification on cotton verticillium wilt based on spectral and image feature fusion
Verticillium wilt is the major disease of cotton, which would cause serious yield reduction and economic losses, and the identification of cotton verticillium wilt is of great significance to cotton research. ... (Source: Plant Methods)
Source: Plant Methods - July 29, 2023 Category: Biology Authors: Zhihao Lu, Shihao Huang, Xiaojun Zhang, Yuxuan shi, Wanneng Yang, Longfu Zhu and Chenglong Huang Tags: Research Source Type: research
One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring F örster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two flu... (Source: Plant Methods)
Source: Plant Methods - July 28, 2023 Category: Biology Authors: Jan Eric Maika, Benedikt Kr ämer, Vivien I. Strotmann, Frank Wellmer, Stefanie Weidtkamp-Peters, Yvonne Stahl and Rüdiger Simon Tags: Methodology Source Type: research
Mapping intron retention events contributing to complex traits using splice quantitative trait locus
Alternative splicing (AS) of mRNA plays an important roles in transcriptome diversity, involving regulation of plant growth and stress response. Understanding the variation of AS events underlying GWAS loci in... (Source: Plant Methods)
Source: Plant Methods - July 21, 2023 Category: Biology Authors: Siyuan Wang, Hongyu Wu, Yongyan Zhao, Luyao Wang, Xueying Guan and Ting Zhao Tags: Research Source Type: research
