A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants
PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality... (Source: Plant Methods)
Source: Plant Methods - April 29, 2023 Category: Biology Authors: Kanae Nishii, Michael M öller, Robert G. Foster, Laura L. Forrest, Nathan Kelso, Sadie Barber, Caroline Howard and Michelle L. Hart Tags: Methodology Source Type: research
Automatic rape flower cluster counting method based on low-cost labelling and UAV-RGB images
The flowering period is a critical time for the growth of rape plants. Counting rape flower clusters can help farmers to predict the yield information of the corresponding rape fields. However, counting in-fie... (Source: Plant Methods)
Source: Plant Methods - April 24, 2023 Category: Biology Authors: Jie Li, Enguo Wang, Jiangwei Qiao, Yi Li, Li Li, Jian Yao and Guisheng Liao Tags: Methodology Source Type: research
Field-measured canopy height may not be as accurate and heritable as believed: evidence from advanced 3D sensing
Canopy height (CH) is an important trait for crop breeding and production. The rapid development of 3D sensing technologies shed new light on high-throughput height measurement. However, a systematic compariso... (Source: Plant Methods)
Source: Plant Methods - April 2, 2023 Category: Biology Authors: Jingrong Zang, Shichao Jin, Songyin Zhang, Qing Li, Yue Mu, Ziyu Li, Shaochen Li, Xiao Wang, Yanjun Su and Dong Jiang Tags: Research Source Type: research
Analysis of plant cuticles and their interactions with agrochemical surfactants using a 3D printed diffusion chamber
Decades of research is available on their effects of single component surfactant on active ingredient diffusion across plant cuticular membranes, but ingredient diffusion is rarely analysed in the presence of ... (Source: Plant Methods)
Source: Plant Methods - April 1, 2023 Category: Biology Authors: Lakshmi Venkatesha Manyu Vittal, James Rookes, Ben Boyd and David Cahill Tags: Methodology Source Type: research
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning
The identification and enumeration of medicinal plants at high elevations is an important part of accurate yield calculations. However, the current assessment of medicinal plant reserves continues to rely on f... (Source: Plant Methods)
Source: Plant Methods - April 1, 2023 Category: Biology Authors: Rong Ding, Jiawei Luo, Chenghui Wang, Lianhui Yu, Jiangkai Yang, Meng Wang, Shihong Zhong and Rui Gu Tags: Research Source Type: research
Workflow for phenotyping sugar beet roots by automated evaluation of cell characteristics and tissue arrangement using digital image processing
Cell characteristics, including cell type, size, shape, packing, cell-to-cell-adhesion, intercellular space, and cell wall thickness, influence the physical characteristics of plant tissues. Genotypic differen... (Source: Plant Methods)
Source: Plant Methods - March 31, 2023 Category: Biology Authors: Nelia Nause, Facundo R. Ispizua Yamati, Marion Seidel, Anne-Katrin Mahlein and Christa M. Hoffmann Tags: Methodology Source Type: research
Rapid non-destructive method to phenotype stomatal traits
Stomata are tiny pores on the leaf surface that are central to gas exchange. Stomatal number, size and aperture are key determinants of plant transpiration and photosynthesis, and variation in these traits can... (Source: Plant Methods)
Source: Plant Methods - March 31, 2023 Category: Biology Authors: Phetdalaphone Pathoumthong, Zhen Zhang, Stuart J. Roy and Abdeljalil El Habti Tags: Methodology Source Type: research
Cotton plant part 3D segmentation and architectural trait extraction using point voxel convolutional neural networks
Plant architecture can influence crop yield and quality. Manual extraction of architectural traits is, however, time-consuming, tedious, and error prone. The trait estimation from 3D data addresses occlusion i... (Source: Plant Methods)
Source: Plant Methods - March 30, 2023 Category: Biology Authors: Farah Saeed, Shangpeng Sun, Javier Rodriguez-Sanchez, John Snider, Tianming Liu and Changying Li Tags: Research Source Type: research
Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS
Insertion of Mg2+ into protoporphyrin IX (PPIX) to produce magnesium-protoporphyrin IX (Mg-PPIX) was the first step toward chlorophyll biosynthesis, which not only imparts plants green pigmentation but underpins ... (Source: Plant Methods)
Source: Plant Methods - March 30, 2023 Category: Biology Authors: Chenyu Zhang, Chunlei Ma, Li Zhu and Mingzhe Yao Tags: Methodology Source Type: research
Fast-track transformation and genome editing in Brachypodium distachyon
Even for easy-to-transform species or genotypes, the creation of transgenic or edited plant lines remains a significant bottleneck. Thus, any technical advance that accelerates the regeneration and transformat... (Source: Plant Methods)
Source: Plant Methods - March 29, 2023 Category: Biology Authors: Camille Soulhat, Houssein Wehbi, Yannick Fierlej, Patrick Berquin, Thomas Girin, Pierre Hilson and Oumaya Bouchabk é-Coussa Tags: Methodology Source Type: research
Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves
Necrotizing pathogens pose an immense economic and ecological threat to trees and forests, but the molecular analysis of these pathogens is still in its infancy because of lacking model systems. To close this ... (Source: Plant Methods)
Source: Plant Methods - March 29, 2023 Category: Biology Authors: Steven Dreischhoff, Ishani Shankar Das, Felix H äffner, Anna Malvine Wolf, Andrea Polle and Karl Henrik Kasper Tags: Methodology Source Type: research
TSWIFT: Tower Spectrometer on Wheels for Investigating Frequent Timeseries for high-throughput phenotyping of vegetation physiology
Remote sensing instruments enable high-throughput phenotyping of plant traits and stress resilience across scale. Spatial (handheld devices, towers, drones, airborne, and satellites) and temporal (continuous o... (Source: Plant Methods)
Source: Plant Methods - March 28, 2023 Category: Biology Authors: Christopher Y. S. Wong, Taylor Jones, Devin P. McHugh, Matthew E. Gilbert, Paul Gepts, Antonia Palkovic, Thomas N. Buckley and Troy S. Magney Tags: Methodology Source Type: research
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two
In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-cod... (Source: Plant Methods)
Source: Plant Methods - March 28, 2023 Category: Biology Authors: Jana Ordon, Niklas Kiel, Dieter Becker, Carola Kretschmer, Paul Schulze-Lefert and Johannes Stuttmann Tags: Methodology Source Type: research
Correction: A modified aeroponic system for growing small-seeded legumes and other plants to study root systems
(Source: Plant Methods)
Source: Plant Methods - March 25, 2023 Category: Biology Authors: Jingya Cai, Vijaykumar Veerappan, Kate Arildsen, Catrina Sullivan, Megan Piechowicz, Julia Frugoli and Rebecca Dickstein Tags: Correction Source Type: research
Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues
Plant cells usually sustain a stable membrane potential due to influx and/or efflux of charged ions across plasma membrane. With the growth and development of plants, different tissues and cells undergo system... (Source: Plant Methods)
Source: Plant Methods - March 21, 2023 Category: Biology Authors: Hai Liu, Yufei Li, Ting Peng and Shaowu Xue Tags: Research Source Type: research
