Correction: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture
(Source: Plant Methods)
Source: Plant Methods - November 25, 2023 Category: Biology Authors: Hans Bethge, Traud Winkelmann, Patrick L üdeke and Thomas Rath Tags: Correction Source Type: research

Monitoring of drought stress and transpiration rate using proximal thermal and hyperspectral imaging in an indoor automated plant phenotyping platform
Thermography is a popular tool to assess plant water-use behavior, as plant temperature is influenced by transpiration rate, and is commonly used in field experiments to detect plant water deficit. Its applica... (Source: Plant Methods)
Source: Plant Methods - November 23, 2023 Category: Biology Authors: Stien Mertens, Lennart Verbraeken, Heike Sprenger, Sam De Meyer, Kirin Demuynck, Bernard Cannoot, Julie Merchie, Jolien De Block, Jonathan T. Vogel, Wesley Bruce, Hilde Nelissen, Steven Maere, Dirk Inz é and Nathalie Wuyts Tags: Research Source Type: research

A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, ... (Source: Plant Methods)
Source: Plant Methods - November 22, 2023 Category: Biology Authors: Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum and Doris Wagner Tags: Methodology Source Type: research

Tensor methods in data analysis of chromatography/mass spectroscopy-based plant metabolomics
Plant metabolomics is an important research area in plant science. Chemometrics is a useful tool for plant metabolomic data analysis and processing. Among them, high-order chemometrics represented by tensor mo... (Source: Plant Methods)
Source: Plant Methods - November 21, 2023 Category: Biology Authors: Lili Guo, Huiwen Yu, Yuan Li, Chenxi Zhang and Mourad Kharbach Tags: Review Source Type: research

How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis
The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem... (Source: Plant Methods)
Source: Plant Methods - November 19, 2023 Category: Biology Authors: Karolina Ku łak, Natalia Wojciechowska, Anna Samelak-Czajka, Paulina Jackowiak and Agnieszka Bagniewska-Zadworna Tags: Review Source Type: research

TreeJ: an ImageJ plugin for interactive cell lineage reconstruction from static images
With the emergence of deep-learning methods, tools are needed to capture and standardize image annotations made by experimentalists. In developmental biology, cell lineages are generally reconstructed from tim... (Source: Plant Methods)
Source: Plant Methods - November 16, 2023 Category: Biology Authors: Elise Laruelle, Jean-Christophe Palauqui, Philippe Andrey and Alain Trubuil Tags: Software Source Type: research

Free and open-source software for object detection, size, and colour determination for use in plant phenotyping
Object detection, size determination, and colour detection of images are tools commonly used in plant science. Key examples of this include identification of ripening stages of fruit such as tomatoes and the d... (Source: Plant Methods)
Source: Plant Methods - November 15, 2023 Category: Biology Authors: Harry Charles Wright, Frederick Antonio Lawrence, Anthony John Ryan and Duncan Drummond Cameron Tags: Methodology Source Type: research

A non-invasive method to predict drought survival in Arabidopsis using quantum yield under light conditions
Survival rate (SR) is frequently used to compare drought tolerance among plant genotypes. While a variety of techniques for evaluating the stress status of plants under drought stress conditions have been deve... (Source: Plant Methods)
Source: Plant Methods - November 15, 2023 Category: Biology Authors: Thelma Y. Rico-Cambron, Elohim Bello-Bello, Octavio Mart ínez and Luis Herrera-Estrella Tags: Methodology Source Type: research

SpykProps: an imaging pipeline to quantify architecture in unilateral grass inflorescences
Inflorescence properties such length, spikelet number, and their spatial distribution across the rachis, are fundamental indicators of seed productivity in grasses and have been a target of selection throughou... (Source: Plant Methods)
Source: Plant Methods - November 13, 2023 Category: Biology Authors: Joan Barreto Ortiz, Candice N. Hirsch, Nancy Jo Ehlke and Eric Watkins Tags: Methodology Source Type: research

A comparative study on point cloud down-sampling strategies for deep learning-based crop organ segmentation
The 3D crop data obtained during cultivation is of great significance to screening excellent varieties in modern breeding and improvement on crop yield. With the rapid development of deep learning, researchers... (Source: Plant Methods)
Source: Plant Methods - November 11, 2023 Category: Biology Authors: Dawei Li, Yongchang Wei and Rongsheng Zhu Tags: Research Source Type: research

Machine learning provides specific detection of salt and drought stresses in cucumber based on miRNA characteristics
This study introduces a novel method to detect the type and severity of stress in c... (Source: Plant Methods)
Source: Plant Methods - November 8, 2023 Category: Biology Authors: Parvin Mohammadi and Keyvan Asefpour Vakilian Tags: Research Source Type: research

Semantic segmentation of plant roots from RGB (mini-) rhizotron images —generalisation potential and false positives of established methods and advanced deep-learning models
Manual analysis of (mini-)rhizotron (MR) images is tedious. Several methods have been proposed for semantic root segmentation based on homogeneous, single-source MR datasets. Recent advances in deep learning (... (Source: Plant Methods)
Source: Plant Methods - November 6, 2023 Category: Biology Authors: Pavel Baykalov, Bart Bussmann, Richard Nair, Abraham George Smith, Gernot Bodner, Ofer Hadar, Naftali Lazarovitch and Boris Rewald Tags: Methodology Source Type: research

Optimizing the photon ratio of red, green, and blue LEDs for lettuce seedlings: a mixture design approach
Light control technology has been developed and studied for decades in controlled environment agriculture (CEA) for successful crop production. The effects of the light spectrum on plant growth can vary becaus... (Source: Plant Methods)
Source: Plant Methods - November 5, 2023 Category: Biology Authors: Eunjeong Lim, Jong-Ok Kim and Myung-Min Oh Tags: Research Source Type: research

Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples
The mutualistic interaction between entomophilous plants and pollinators is fundamental to the structure of most terrestrial ecosystems. The sensitive nature of this relationship has been disrupted by anthropo... (Source: Plant Methods)
Source: Plant Methods - November 4, 2023 Category: Biology Authors: Sydney B. Wizenberg, Laura R. Newburn, Mateus Pepinelli, Ida M. Conflitti, Rodney T. Richardson, Shelley E. R. Hoover, Robert W. Currie, Pierre Giovenazzo and Amro Zayed Tags: Methodology Source Type: research

Acceleration of wheat breeding: enhancing efficiency and practical application of the speed breeding system
Crop breeding should be accelerated to address global warming and climate change. Wheat (Triticum aestivum L.) is a major food crop. Speed breeding (SB) and speed vernalization (SV) techniques for spring and wint... (Source: Plant Methods)
Source: Plant Methods - November 4, 2023 Category: Biology Authors: Jin-Kyung Cha, Hyeonjin Park, Changhyun Choi, Youngho Kwon, So-Myeong Lee, Ki-Won Oh, Jong-Min Ko, Soon-Wook Kwon and Jong-Hee Lee Tags: Research Source Type: research