Ripening dynamics revisited: an automated method to track the development of asynchronous berries on time-lapse images
Grapevine berries undergo asynchronous growth and ripening dynamics within the same bunch. Due to the lack of efficient methods to perform sequential non-destructive measurements on a representative number of ... (Source: Plant Methods)
Source: Plant Methods - December 14, 2023 Category: Biology Authors: Benoit Daviet, Christian Fournier, Lloren ç Cabrera-Bosquet, Thierry Simonneau, Maxence Cafier and Charles Romieu Tags: Methodology Source Type: research

A deep learning model for predicting risks of crop pests and diseases from sequential environmental data
Crop pests reduce productivity, so managing them through early detection and prevention is essential. Data from various modalities are being used to predict crop diseases by applying machine learning methodolo... (Source: Plant Methods)
Source: Plant Methods - December 14, 2023 Category: Biology Authors: Sangyeon Lee and Choa Mun Yun Tags: Research Source Type: research

A protocol for a turbidimetric assay using a Saccharomyces cerevisiae thiamin biosynthesis mutant to estimate total vitamin B1 content in plant tissue samples
Understanding thiamin (thiamine; vitamin B1) metabolism in plants is crucial, as it impacts plant nutritional value as well as stress tolerance. Studies aimed at elucidating novel aspects of thiamin in plants rel... (Source: Plant Methods)
Source: Plant Methods - December 13, 2023 Category: Biology Authors: Simon Strobbe, Jana Verstraete, Teresa B. Fitzpatrick, Christophe Stove and Dominique Van Der Straeten Tags: Methodology Source Type: research

Seed protein biotyping in Amaranthus species: a tool for rapid identification of weedy amaranths of concern
The Amaranthus genus contains at least 20 weedy and invasive species, including Amaranthus palmeri (palmer ’s amaranth) and Amaranthus tuberculatus (tall waterhemp), two species of regulatory concern in North Amer... (Source: Plant Methods)
Source: Plant Methods - December 11, 2023 Category: Biology Authors: Maxime Murphy, Julia Hubert, Ruojing Wang and Leonardo Galindo-Gonz ález Tags: Methodology Source Type: research

Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation
Plant defense activators offer advantages over pesticides by avoiding the emergence of drug-resistant pathogens. However, only a limited number of compounds have been reported. Reactive oxygen species (ROS) ac... (Source: Plant Methods)
Source: Plant Methods - December 8, 2023 Category: Biology Authors: Masayuki Kogoshi, Daiki Nishio, Nobutaka Kitahata, Hayato Ohwada, Kazuyuki Kuchitsu, Hideyuki Mizuno and Takamitsu Kurusu Tags: Research Source Type: research

Pre-trained protein language model sheds new light on the prediction of Arabidopsis protein –protein interactions
Protein –protein interactions (PPIs) are heavily involved in many biological processes. Consequently, the identification of PPIs in the model plant Arabidopsis is of great significance to deeply understand plan... (Source: Plant Methods)
Source: Plant Methods - December 7, 2023 Category: Biology Authors: Kewei Zhou, Chenping Lei, Jingyan Zheng, Yan Huang and Ziding Zhang Tags: Research Source Type: research

Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina
Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are ... (Source: Plant Methods)
Source: Plant Methods - December 6, 2023 Category: Biology Authors: Xin Li, Victoria Yell and Xu Li Tags: Research Source Type: research

Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes
Plant-parasitic nematodes compromise the agriculture of a wide variety of the most common crops worldwide. Obtaining information on the fundamental biology of these organisms and how they infect the plant has ... (Source: Plant Methods)
Source: Plant Methods - December 4, 2023 Category: Biology Authors: Alexis L. Sperling and Sebastian Eves-van den Akker Tags: Methodology Source Type: research

An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus
For molecular breeding of future apples, wild apple (Malus sieversii), the primary progenitor of domesticated apples, provides abundant genetic diversity and disease-resistance traits. Valsa canker (caused by the... (Source: Plant Methods)
Source: Plant Methods - December 2, 2023 Category: Biology Authors: Xuejing Wen, Jiangxue Yuan, Tohir A. Bozorov, Abdul Waheed, Gulnaz Kahar, Yakupjan Haxim, Xiaojie Liu, Lili Huang and Daoyuan Zhang Tags: Methodology Source Type: research

A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages
Doubled haploid technology, which enables the generation of homozygous lines in a single step, is one of the modern tools being employed for accelerating breeding processes in different crops. In rice, a globa... (Source: Plant Methods)
Source: Plant Methods - December 1, 2023 Category: Biology Authors: Chaitanya Ghalagi, Malavalli Rajashekar Namratha, Kavita Kotyal, Shiva Prakash and Basavaiah Mohan Raju Tags: Research Source Type: research

Regulating bulb dormancy release and flowering in lily through chemical modulation of intercellular communication
In this study, we identified three regulators of... (Source: Plant Methods)
Source: Plant Methods - November 28, 2023 Category: Biology Authors: Yajie Zhao, Wenqiang Pan, Yin Xin, Jingxiang Wu, Rong Li, Jinxin Shi, Shuo Long, Lianwei Qu, Yingdong Yang, Mingfang Yi and Jian Wu Tags: Research Source Type: research

A highlightedly improved method for isolating and characterizing calcium oxalate crystals from tubercles of Mammillaria schumannii
Calcium oxalate (CaOx) is the most prevalent and widespread biomineral in plants and is involved in protective and/or defensive functions against abiotic stress factors. It is, however, expected that this func... (Source: Plant Methods)
Source: Plant Methods - November 27, 2023 Category: Biology Authors: Changying Li, Chunli Chen, Lihong Qin, Dengyue Zheng, Qian Du, Qiandong Hou and Xiaopeng Wen Tags: Methodology Source Type: research

Comparison of two novel methods for counting wheat ears in the field with terrestrial LiDAR
The metrics for assessing the yield of crops in the field include the number of ears per unit area, the grain number per ear, and the thousand-grain weight. Typically, the ear number per unit area contributes ... (Source: Plant Methods)
Source: Plant Methods - November 26, 2023 Category: Biology Authors: Yangyang Gu, Hongxu Ai, Tai Guo, Peng Liu, Yongqing Wang, Hengbiao Zheng, Tao Cheng, Yan Zhu, Weixing Cao and Xia Yao Tags: Research Source Type: research

Correction: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture
(Source: Plant Methods)
Source: Plant Methods - November 25, 2023 Category: Biology Authors: Hans Bethge, Traud Winkelmann, Patrick L üdeke and Thomas Rath Tags: Correction Source Type: research

Monitoring of drought stress and transpiration rate using proximal thermal and hyperspectral imaging in an indoor automated plant phenotyping platform
Thermography is a popular tool to assess plant water-use behavior, as plant temperature is influenced by transpiration rate, and is commonly used in field experiments to detect plant water deficit. Its applica... (Source: Plant Methods)
Source: Plant Methods - November 23, 2023 Category: Biology Authors: Stien Mertens, Lennart Verbraeken, Heike Sprenger, Sam De Meyer, Kirin Demuynck, Bernard Cannoot, Julie Merchie, Jolien De Block, Jonathan T. Vogel, Wesley Bruce, Hilde Nelissen, Steven Maere, Dirk Inz é and Nathalie Wuyts Tags: Research Source Type: research