X-ray fluorescence spectroscopy (XRF) for metallome analysis of herbarium specimens
“Herbarium X-ray Fluorescence (XRF) Ionomics” is a new quantitative approach for extracting the elemental concentrations from herbarium specimens using handheld XRF devices. These instruments are principally d... (Source: Plant Methods)
Source: Plant Methods - December 19, 2022 Category: Biology Authors: Imam Purwadi, Lachlan W. Casey, Chris G. Ryan, Peter D. Erskine and Antony van der Ent Tags: Methodology Source Type: research
CropPainter: an effective and precise tool for trait-to-image crop visualization based on generative adversarial networks
Virtual plants can simulate the plant growth and development process through computer modeling, which assists in revealing plant growth and development patterns. Virtual plant visualization technology is a cor... (Source: Plant Methods)
Source: Plant Methods - December 15, 2022 Category: Biology Authors: Lingfeng Duan, Zhihao Wang, Hongfei Chen, Jinyang Fu, Hanzhi Wei, Zedong Geng and Wanneng Yang Tags: Research Source Type: research
Pseudo high-frequency boosts the generalization of a convolutional neural network for cassava disease detection
Frequency is essential in signal transmission, especially in convolutional neural networks. It is vital to maintain the signal frequency in the neural network to maintain the performance of a convolutional neu... (Source: Plant Methods)
Source: Plant Methods - December 14, 2022 Category: Biology Authors: Jiayu Zhang, Chao Qi, Peter Mecha, Yi Zuo, Zongyou Ben, Haolu Liu and Kunjie Chen Tags: Research Source Type: research
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants
Long-read sequencing platforms offered by Oxford Nanopore Technologies (ONT) allow native DNA containing epigenetic modifications to be directly sequenced, but can be limited by lower per-base accuracies. A ke... (Source: Plant Methods)
Source: Plant Methods - December 14, 2022 Category: Biology Authors: Scott Ferguson, Todd McLay, Rose L. Andrew, Jeremy J. Bruhl, Benjamin Schwessinger, Justin Borevitz and Ashley Jones Tags: Methodology Source Type: research
A low-cost and open-source solution to automate imaging and analysis of cyst nematode infection assays for Arabidopsis thaliana
Cyst nematodes are one of the major groups of plant-parasitic nematode, responsible for considerable crop losses worldwide. Improving genetic resources, and therefore resistant cultivars, is an ongoing focus o... (Source: Plant Methods)
Source: Plant Methods - December 12, 2022 Category: Biology Authors: Olaf Prosper Kranse, Itsuhiro Ko, Roberta Healey, Unnati Sonawala, Siyuan Wei, Beatrice Senatori, Francesco De Batt é, Ji Zhou and Sebastian Eves-van den Akker Tags: Methodology Source Type: research
A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis
Chromatin-bound RNAs are the primary product of transcription that undergo on-chromatin processing such as capping, splicing, and polyadenylation. These processing steps then determine the fate of RNAs. Albeit... (Source: Plant Methods)
Source: Plant Methods - December 12, 2022 Category: Biology Authors: Qiqi Zhang, Fengli Zhao, Zhe Wu and Danling Zhu Tags: Methodology Source Type: research
IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction
Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amoun... (Source: Plant Methods)
Source: Plant Methods - December 9, 2022 Category: Biology Authors: Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn, Hoo Sun Chung, Dirk Inz é and Stephen Depuydt Tags: Methodology Source Type: research
Four-dimensional measurement of root system development using time-series three-dimensional volumetric data analysis by backward prediction
Root system architecture (RSA) is an essential characteristic for efficient water and nutrient absorption in terrestrial plants; its plasticity enables plants to respond to different soil environments. Better ... (Source: Plant Methods)
Source: Plant Methods - December 9, 2022 Category: Biology Authors: Shota Teramoto and Yusaku Uga Tags: Methodology Source Type: research
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography
The technology of cotton defoliation is essential for mechanical cotton harvesting. Agricultural unmanned aerial vehicle (UAV) spraying has the advantages of low cost, high efficiency and no mechanical damage ... (Source: Plant Methods)
Source: Plant Methods - December 8, 2022 Category: Biology Authors: Jinyong Wu, Sheng Wen, Yubin Lan, Xuanchun Yin, Jiantao Zhang and Yufeng Ge Tags: Research Source Type: research
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time
High-throughput phenotyping platforms allow the study of the form and function of a large number of genotypes subjected to different growing conditions (GxE). A number of image acquisition and processing pipel... (Source: Plant Methods)
Source: Plant Methods - December 8, 2022 Category: Biology Authors: Benoit Daviet, Romain Fernandez, Lloren ç Cabrera-Bosquet, Christophe Pradal and Christian Fournier Tags: Methodology Source Type: research
A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage
Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in ... (Source: Plant Methods)
Source: Plant Methods - December 8, 2022 Category: Biology Authors: Thibault Barrit, Claire Campion, Sophie Aligon, Julie Bourbeillon, David Rousseau, Elisabeth Planchet and B éatrice Teulat Tags: Research Source Type: research
A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes
As complete and accurate genome sequences are becoming easier to obtain, more researchers wish to get one or more of them to support their research endeavors. Reliable and well-documented sequence assembly wor... (Source: Plant Methods)
Source: Plant Methods - December 2, 2022 Category: Biology Authors: Marina P üpke Marone, Harmeet Chawla Singh, Curtis J. Pozniak and Martin Mascher Tags: Methodology Source Type: research
High-throughput and automatic structural and developmental root phenotyping on Arabidopsis seedlings
High-throughput phenotyping is crucial for the genetic and molecular understanding of adaptive root system development. In recent years, imaging automata have been developed to acquire the root system architec... (Source: Plant Methods)
Source: Plant Methods - December 1, 2022 Category: Biology Authors: Romain Fernandez, Amandine Crabos, Morgan Maillard, Philippe Nacry and Christophe Pradal Tags: Methodology Source Type: research
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction
Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impa... (Source: Plant Methods)
Source: Plant Methods - November 28, 2022 Category: Biology Authors: Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher, Zvi Peleg, Paul Staswick, Jason Hupp, Hongfeng Yu and Harkamal Walia Tags: Methodology Source Type: research
A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile
Grasses internodes are made of distinct tissues such as vascular bundles, epidermis, rind and pith. The histology of grasses stem was largely revisited recently taking advantage of the development of microscop... (Source: Plant Methods)
Source: Plant Methods - November 24, 2022 Category: Biology Authors: P.-L. Lopez-Marnet, S. Guillaume, V. M échin and M. Reymond Tags: Methodology Source Type: research
