A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning
Variety genuineness and purity are essential indices of maize seed quality that affect yield. However, detection methods for variety genuineness are time-consuming, expensive, require extensive training, or de... (Source: Plant Methods)
Source: Plant Methods - June 11, 2022 Category: Biology Authors: Keling Tu, Shaozhe Wen, Ying Cheng, Yanan Xu, Tong Pan, Haonan Hou, Riliang Gu, Jianhua Wang, Fengge Wang and Qun Sun Tags: Research Source Type: research
Laser-based molecular delivery and its applications in plant science
Lasers enable modification of living and non-living matter with submicron precision in a contact-free manner which has raised the interest of researchers for decades. Accordingly, laser technologies have drawn... (Source: Plant Methods)
Source: Plant Methods - June 11, 2022 Category: Biology Authors: Dag Heinemann, Miroslav Zabic, Mitsuhiro Terakawa and Jens Boch Tags: Review Source Type: research
Evaluation of an intelligent artificial climate chamber for high-throughput crop phenotyping in wheat
The superposition of COVID-19 and climate change has brought great challenges to global food security. As a major economic crop in the world, studying its phenotype to cultivate high-quality wheat varieties is... (Source: Plant Methods)
Source: Plant Methods - June 7, 2022 Category: Biology Authors: Anhua Ren, Dong Jiang, Min Kang, Jie Wu, Fangcheng Xiao, Pei Hou and Xiuqing Fu Tags: Research Source Type: research
X-ray driven peanut trait estimation: computer vision aided agri-system transformation
In India, raw peanuts are obtained by aggregators from smallholder farms in the form of whole pods and the price is based on a manual estimation of basic peanut pod and kernel characteristics. These methods of... (Source: Plant Methods)
Source: Plant Methods - June 6, 2022 Category: Biology Authors: Martha Domhoefer, Debarati Chakraborty, Eva Hufnagel, Joelle Clau ßen, Norbert Wörlein, Marijn Voorhaar, Krithika Anbazhagan, Sunita Choudhary, Janila Pasupuleti, Rekha Baddam, Jana Kholova and Stefan Gerth Tags: Research Source Type: research
A plant tethering system for the functional study of protein-RNA interactions in vivo
The sorting of RNA transcripts dictates their ultimate post-transcriptional fates, such as translation, decay or degradation by RNA interference (RNAi). This sorting of RNAs into distinct fates is mediated by ... (Source: Plant Methods)
Source: Plant Methods - June 4, 2022 Category: Biology Authors: Diego Cuerda-Gil, Yu-Hung Hung, Kaushik Panda and R. Keith Slotkin Tags: Methodology Source Type: research
WeCoNET: a host –pathogen interactome database for deciphering crucial molecular networks of wheat-common bunt cross-talk mechanisms
Triticum aestivum is the most important staple food grain of the world. In recent years, the outbreak of a major seed-borne disease, common bunt, in wheat resulted in reduced quality and quantity of the crop. The... (Source: Plant Methods)
Source: Plant Methods - June 3, 2022 Category: Biology Authors: Raghav Kataria and Rakesh Kaundal Tags: Database Source Type: research
Experimental data manipulations to assess performance of hyperspectral classification models of crop seeds and other objects
Optical sensing solutions are being developed and adopted to classify a wide range of biological objects, including crop seeds. Performance assessment of optical classification models remains both a priority a... (Source: Plant Methods)
Source: Plant Methods - June 3, 2022 Category: Biology Authors: Christian Nansen, Mohammad S. Imtiaz, Mohsen B. Mesgaran and Hyoseok Lee Tags: Research Source Type: research
Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)
Although the genome for the allotetraploid bioenergy crop switchgrass (Panicum virgatum) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimizatio... (Source: Plant Methods)
Source: Plant Methods - May 30, 2022 Category: Biology Authors: Kira Tiedge, Janessa Destremps, Janet Solano-Sanchez, Magda Lisette Arce-Rodriguez and Philipp Zerbe Tags: Methodology Source Type: research
A method to experimentally clamp leaf water content to defined values to assess its effects on apoplastic pH
Leaf hydration is controlled by feedback mechanisms, e.g. stomatal responses, adjustments of osmotic potential and hydraulic conductivity. Leaf water content thus is an input into related feedback-loops contro... (Source: Plant Methods)
Source: Plant Methods - May 30, 2022 Category: Biology Authors: Hartmut Kaiser, Amit Sagervanshi and Karl H. M ühling Tags: Methodology Source Type: research
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein –protein interactions
The bimolecular fluorescence complementation (BiFC) assay has emerged as one of the most popular methods for analysing protein –protein interactions (PPIs) in plant biology. This includes its increasing use as ... (Source: Plant Methods)
Source: Plant Methods - May 26, 2022 Category: Biology Authors: Florent Velay, M élanie Soula, Marwa Mehrez, Clément Belbachir, Stefano D’Alessandro, Christophe Laloi, Patrice Crete and Ben Field Tags: Methodology Source Type: research
Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR
Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure... (Source: Plant Methods)
Source: Plant Methods - May 26, 2022 Category: Biology Authors: Yin Xinying, Li Xin, Yang Lili, Zheng Qiuyue, Piao Yongzhe and Cao Jijuan Tags: Research Source Type: research
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery
Leaf Area Index (LAI) is half of the amount of leaf area per unit horizontal ground surface area. Consequently, accurate vegetation extraction in remote sensing imagery is critical for LAI estimation. However,... (Source: Plant Methods)
Source: Plant Methods - May 19, 2022 Category: Biology Authors: Shuang Wu, Lei Deng, Lijie Guo and Yanjie Wu Tags: Research Source Type: research
Determination of protoplast growth properties using quantitative single-cell tracking analysis
Although quantitative single-cell analysis is frequently applied in animal systems, e.g. to identify novel drugs, similar applications on plant single cells are largely missing. We have exploited the applicabi... (Source: Plant Methods)
Source: Plant Methods - May 18, 2022 Category: Biology Authors: Jonathan Dawson, Saurabh Pandey, Qiuju Yu, Patrick Schaub, Florian W üst, Amir Bahram Moradi, Oleksandr Dovzhenko, Klaus Palme and Ralf Welsch Tags: Research Source Type: research
A protocol for Chenopodium quinoa pollen germination
Quinoa is an increasingly popular seed crop frequently studied for its tolerance to various abiotic stresses as well as its susceptibility to heat. Estimations of quinoa pollen viability through staining metho... (Source: Plant Methods)
Source: Plant Methods - May 18, 2022 Category: Biology Authors: S. Elizabeth Castillo, Jose C. Tovar, Anastasia Shamin, Jorge Gutirerrez, Paige Pearson and Malia A. Gehan Tags: Methodology Source Type: research
Banana plant counting and morphological parameters measurement based on terrestrial laser scanning
The number of banana plants is closely related to banana yield. The diameter and height of the pseudo-stem are important morphological parameters of banana plants, which can reflect the growth status and vital... (Source: Plant Methods)
Source: Plant Methods - May 18, 2022 Category: Biology Authors: Yanlong Miao, Liuyang Wang, Cheng Peng, Han Li, Xiuhua Li and Man Zhang Tags: Research Source Type: research
