Comparative study of the ameliorative effects of omega-3 versus selenium on etoposide-induced changes in Sertoli cells and ectoplasmic specialization of adult rat testes: immunohistochemical and electron microscopic study
AbstractEtoposide (Eto) is an anti-cancer drug that is associated with serious adverse effects on male reproductive function. Omega-3 polyunsaturated fatty acids ( ω-3 PUFAs) and selenium (Se) are known as anti-inflammatory, anti-apoptotic and anti-oxidant agents. This work was designed to investigate changes in the biochemical parameters as well as alterations in Sertoli cell vimentin expression, ultrastructure and ectoplasmic specializations (ESs) following Eto treatment and to assess the ameliorative effect of ω-3 versus Se on these alterations. Eighty four adult male albino rats were used and classified into four gro...
Source: Journal of Molecular Histology - February 3, 2022 Category: Laboratory Medicine Source Type: research
Endocrine secretory granule production is caused by a lack of REST and intragranular secretory content and accelerated by PROX1
In this study, we experimentally induced ESGs in the non-neuroendocrine lung cancer cell line H1299. Since repressive element 1 silencing transcription factor (REST) and prospero homeobox 1 (PROX1) are closely associated with the expression of ESG-related molecules, we edited theREST gene and/or transfected PROX1 and then performed molecular biology, immunocytochemistry, and electron and immunoelectron microscopy assays to determine whether ESG-related molecules and ESGs were induced in H1299 cells. Although chromogranin/secretogranin family proteins were induced in H1299 cells by knockout ofREST and the induction was acce...
Source: Journal of Molecular Histology - January 30, 2022 Category: Laboratory Medicine Source Type: research
TGF- β2-induced circ-PRDM5 regulates migration, invasion, and EMT through the miR-92b-3p/COL1A2 pathway in human lens epithelial cells
AbstractCircRNAcirc-PRDM5 (PR/SET domain 5) (circ-PRDM5) is overexpressed in age-related cataracts. Nevertheless, the biological role of circ-PRDM5 in posterior capsule opacities (PCO) (a common complication after cataract surgery) is unclear. Human lens epithelial cells SRA01/04 (LECs) were stimulated with TGF- β2 (transforming growth factor beta-2) to mimic the PCO model in vitro. Cell viability, migration, and invasion were determined by MTT, transwell, or wound-healing assays. Protein levels of EMT (epithelial-to-mesenchymal transition) markers andCOL1A2 (collagen type I alpha 2 chain) were analyzed by western blottin...
Source: Journal of Molecular Histology - January 27, 2022 Category: Laboratory Medicine Source Type: research
Effects of PHD and HSP90 on erythropoietin production in yak (Bos grunniens) renal interstitial fibroblast-like cells under hypoxia
In conclusion, these findings support our proposition that PHD regulates E PO via HIF-2α in yak RIFs, while HSP90 impelled EPO expression. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - January 27, 2022 Category: Laboratory Medicine Source Type: research
MiR-361-3p alleviates cerebral ischemia –reperfusion injury by targeting NACC1 through the PINK1/Parkin pathway
In conclusion, miR-361-3p could improve ischemia brain injury by targeting NACC1 through the PINK1/Parkin pathway. Therefore, miR-361-3p may serve as a potential therapeutic target for the brain injury after I/R. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - January 24, 2022 Category: Laboratory Medicine Source Type: research
Knockdown of lncRNA HAGLR promotes Treg cell differentiation through increasing the RUNX3 level in dermatomyositis
AbstractDermatomyositis (DM) is a systemic autoimmune disease. It ’s known that the number of regulatory T (Treg) cells was decreased in DM. Besides, Treg cells were increased after treatment in DM patients. Forkhead box P3 (Foxp3) is specifically expressed in Treg cells and Runt-related transcription factor (RUNX3) could regulate Foxp3 transcription. And our pr evious experiment showed that Homeobox D gene cluster antisense growth-associated long noncoding RNA (HAGLR) was up-regulated in DM patients. Here, we aimed to explore whether HAGLR regulated the differentiation of Treg cells through RUNX3-mediated transcription ...
Source: Journal of Molecular Histology - January 22, 2022 Category: Laboratory Medicine Source Type: research
Overview of signal transduction between LL37 and bone marrow-derived MSCs
AbstractAs a key signaling molecule, cationic antimicrobial peptide LL37 helps mediate intracellular and extracellular signal transduction. It interacts with various cells facilitating tissue repair and plays a vital role in the defense against pathogens. LL37 acts as a broad-spectrum antimicrobial, possessing antitumor and antiviral properties. It promotes angiogenesis, co-operates with growth factors, antagonizes inflammatory media, participates in immune regulation, and helps tissue repair and growth. These biological effects are closely related to the information exchange between LL37 and various cells, in particular m...
Source: Journal of Molecular Histology - January 20, 2022 Category: Laboratory Medicine Source Type: research
LncRNA OGFRP1 promotes cell proliferation and suppresses cell radiosensitivity in gastric cancer by targeting the miR-149-5p/MAP3K3 axis
AbstractGastric cancer (GC) is an aggressive malignancy with high incidence and mortality. Radiotherapy is a common treatment for patients with advanced GC. Many long noncoding RNAs (lncRNAs) have been verified to affect the radiosensitivity of multiple cancers in previous studies. Nevertheless, whether lncRNA opioid growth factor receptor pseudogene 1 (OGFRP1) affects the radiosensitivity of GC has not been determined. We hypothesized that OGFRP1 might affect cellular processes in GC development. The present study aims to explore the role of OGFRP1 in GC development. First, high expression of OGFRP1 in GC tissues and cell...
Source: Journal of Molecular Histology - January 20, 2022 Category: Laboratory Medicine Source Type: research
Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation
AbstractThe Yes-associated protein (YAP) transcription co-activator is recognized as a key mediator and has been implicated in the regulation of stem cell fate; however, the role of YAP in the tenogenic differentiation of mesenchymal stem cells (MSCs) is not well understood. In the present study, we characterized tenogenic differentiation of C3H10T1/2 cells induced by BMP-12 and evaluated the function of YAP in the regulation of the BMP-12-induced tenogenesis. We found that the tendon-related proteins Scleraxis, Tenascin C, and Tenomodulin were significantly increased after induction by 10 ng/ml BMP-12 for 48 h in C3H10...
Source: Journal of Molecular Histology - January 19, 2022 Category: Laboratory Medicine Source Type: research
Circ_0003159 upregulates LIFR expression through competitively binding to miR-221-3p/miR-222-3p to block gastric cancer development
In conclusion, circ_0003159 inhibited GC development in vitro and in vivo by enriching the level of LIFR via direct binding to miR-221-3p/miR-222-3p. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - January 16, 2022 Category: Laboratory Medicine Source Type: research
The effects of mesenchymal stem cells transplantation on A1 neurotoxic reactive astrocyte and demyelination in the cuprizone model
AbstractMultiple sclerosis (MS), which is an autoimmune disease, is characterized by symptoms such as demyelination, axonal damage, and astrogliosis. As the most abundant type of glial cells, astrocytes play an important role in MS pathogenesis. Mesenchymal stem cells (MSCs) are a subset of stromal cells that have the potential for migration, immune-modulation, differentiation, remyelination, and neuroregeneration. Therefore, the present study evaluates the effects of MSC transplantation on A1 reactive astrocytes and the remyelination process in the cuprizone mouse model. The study used 30 male C57BL/6 mice, which were ran...
Source: Journal of Molecular Histology - January 15, 2022 Category: Laboratory Medicine Source Type: research
Glucose-6-phosphate dehydrogenase promotes the proliferation and migration of lung adenocarcinoma cells via the STAT3 signaling pathway
In this study, we found that G6PD was significantly upregulated in LUAD specimens and cell lines, and that the high levels of G6PD expression were closely associated with a poor prognosis for LUAD patients. Moreover, we found that G6PD significantly promoted the proliferation and migration of LUAD cells in vitro, and overexpression of G6PD also play a role of facilitating tumorigenesis in in vivo experiments. Mechanistically, the STAT3 signaling pathway was significantly activated by G6PD-mediated LUAD progression. Overall, our results suggest that G6PD could serve as a novel prognostic marker and therapeutic target for tr...
Source: Journal of Molecular Histology - January 14, 2022 Category: Laboratory Medicine Source Type: research
MiR-556-5p modulates migration, invasion, and epithelial-mesenchymal transition in breast cancer cells via targeting PTHrP
AbstractBreast cancer bone metastases may block normal bone remodeling and promote bone degradation, during which several signaling pathways and small non-coding miRNAs might all play a role. miRNAs and target mRNAs that might be associated with breast cancer bone metastasis were analyzed and selected using bioinformatics analyses based on online data. The 3 ′ untranslated region of key factors associated with breast cancer metastasis were examined for candidate miRNA binding site using Targetscan. The predicted binding was validated. The specific effects of single miRNA and dynamic effects of the miRNA-mRNA axis on brea...
Source: Journal of Molecular Histology - January 9, 2022 Category: Laboratory Medicine Source Type: research
MiR-20b-5p contributes to the dysfunction of vascular smooth muscle cells by targeting MAGI3 in hypertension
ConclusionsMiR-20b-5p contributed to the dysfunction of ASMCs by targeting MAGI3 in hypertension. This new discovery provided a potential novel insight for hypertension treatment. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - January 5, 2022 Category: Laboratory Medicine Source Type: research
MircoRNA-126-5p inhibits apoptosis of endothelial cell in vascular arterial walls via NF- κB/PI3K/AKT/mTOR signaling pathway in atherosclerosis
In conclusion, these data indicated that transfection of miR-126-5p rescued apoptosis of HAECs and limited atherosclerosis, introducin g a potential therapeutic approach for atherosclerosis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - January 4, 2022 Category: Laboratory Medicine Source Type: research
