A novel miR-338-3p/SLC1A5 axis reprograms retinal pigment epithelium to increases its resistance to high glucose-induced cell ferroptosis
ConclusionsCollectively, data in the present study indicated that targeting the miR-338-3p/SLC1A5 axis could block high glucose-induced ferroptosis in RPE cells. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 23, 2022 Category: Laboratory Medicine Source Type: research
The ameliorative effects of cinnamon oil against ethanol-induced gastric ulcer in rats by regulating oxidative stress and promoting angiogenesis
AbstractCinnamon is one of the herbal resources belonging to the Lauraceae family, is commonly used in traditional medicine and as a flavoring agent. It has antioxidant and anti-inflammatory activities. Therefore, the present study was performed to evaluate the gastroprotective effect of cinnamon on ethanol-induce d gastric ulcer in comparison to omeprazole. In Wistar rats, gastric ulcers were induced using one oral dose of 70% ethanol (5 ml/kg b. w.) Cinnamon oil at doses of 2.5 ml/kg body weight and omeprazole (a reference drug) at a dose of 20 mg/kg body weight were orally administrated daily for 7 day s before ulc...
Source: Journal of Molecular Histology - March 15, 2022 Category: Laboratory Medicine Source Type: research
The lncRNA MIAT/miR-181a-5p axis regulates osteopontin (OPN)-mediated proliferation and apoptosis of human chondrocytes in osteoarthritis
This study explores the upstream regulatory molecular mechanism of OPN on proliferation and apoptosis of human chondrocytes in OA. Chondrocytes were isolated from OA cartilage and identified by toluidine blue staining and immunofluorescent staining of type II collagen. An MTT assay was used for cell viability, and a BrdU assay was applied for DNA synthesis. Cell apoptosis was detected by a flow cytometry assay. A lncRNA MIAT/miR-181a-5p/OPN axis regulating OA chondrocyte proliferation and apoptosis were identified. miR-181a-5p directly targeted OPN and inhibited OPN expression in OA chondrocytes. miR-181a-5p overexpression...
Source: Journal of Molecular Histology - March 14, 2022 Category: Laboratory Medicine Source Type: research
Comparative microanatomical and histochemical biodistribution profiles of different types of mucins in the intestinal mucosa of some tetrapod representatives
AbstractThe microanatomical features of the intestinal tract mucosa layer in different species of tetrapoda vary according to the type of species, tissue, and function of the targeted cells. In the present study, we have evaluated the histological and histochemical variations of the intestinal tract in four species representing superclass tetrapoda.Bufo regularis (toad),Trachylepis quinquetaeniata (lizard),Columba livia domestica (pigeon) andMus musculus (mouse) were used as representatives for amphibians, reptilians, avians and mammalians respectively. Histologically, the ileum ’s mucosal layer of the lower tetrapods (t...
Source: Journal of Molecular Histology - March 6, 2022 Category: Laboratory Medicine Source Type: research
Silence of MLK3 alleviates lipopolysaccharide-induced lung epithelial cell injury via inhibiting p53-mediated ferroptosis
AbstractAcute lung injury (ALI) is characterized with a high rate of morbidity and mortality. The injury and apoptosis of lung epithelial cells play crucial roles in the progression of ALI. Mixed lineage kinase 3 (MLK3) has been reported to be involved in the regulation of cellular biological functions, such as cell proliferation, apoptosis and ferroptosis. However, the effect of MLK3 exerted on ALI has not been reported. Here, LPS-stimulated MLE12 pulmonary epithelial cells were used as anin vitro model for ALI. In this research, LPS elevated the expression of MLK3 in MLE12 cells. The silence of MLK3 alleviated LPS-induce...
Source: Journal of Molecular Histology - March 5, 2022 Category: Laboratory Medicine Source Type: research
VIP alleviates sepsis-induced cognitive dysfunction as the TLR-4/NF- κB signaling pathway is inhibited in the hippocampus of rats
In conclusion, VIP inhibited neuro inflammation and cognitive impairment in hippocampus of septic rats through the TLR-4/NF-κB signaling pathway. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 3, 2022 Category: Laboratory Medicine Source Type: research
The protective effect of hesperidin on the liver of hypothyroid rats mediated by nuclear factor erythroid 2-related factor 2-dependent activation of heme oxygenase 1
AbstractHypothyroidism (HPO) has been linked to a higher incidence of hepatic lesions. Hesperidin (HSP) is an antioxidant, anti-adipogenic, anti-inflammatory, anti-hyperlipidemic, and anti-apoptotic agent. Therefore, the study aimed to assess the impact of carbimazole (CBZ)-induced HPO on adult albino rats ’ liver and explore the possible ameliorating effect of Eltroxin (ELT) and HSP. HPO was induced by CBZ (20 mg/kg/day). Rats were allocated into group I: normal control; group II: received CBZ (20 mg/kg/day) only; group III: received CBZ and HSP (200 mg/kg/day); and group IV: received CBZ and EL T (0.045 mg/kg/day)....
Source: Journal of Molecular Histology - February 28, 2022 Category: Laboratory Medicine Source Type: research
Intrinsic innervation of the ovary and its variations in the rat senescence process
AbstractOvarian functions decrease with perimenopause. The ovary has extrinsic innervation, but the neural influence on ovarian functions and dysfunction is not well-studied. The present study aimed to biochemically and morphometrically characterize the intrinsic neurons in ovaries from young adult, middle-aged, and senescent Long Evans CII-ZV rats (3, 12, and 15 months old, respectively). Ovaries were extracted from four rats of each age group (n = 12 total), cryopreserved, and processed for immunofluorescence studies with the primary NeuN/β-tubulin and NeuN/tyrosine hydroxylase (TH) antibodies. The soma area and n...
Source: Journal of Molecular Histology - February 25, 2022 Category: Laboratory Medicine Source Type: research
Functional characterization of CD49f+ hepatic stem/progenitor cells in adult mice liver
AbstractHepatic Stem/progenitor cells (HSPCs) have gained a large amount of interest for treating acute liver disease. However, the isolation and identification of HSPCs are unclear due to the lack of cell-specific surface markers. To isolate adult HSPCs, we used cell surface-marking antibodies, including CD49f and Sca-1. Two subsets of putative HSPCs, Lin−CD45−Sca-1−CD49f+ (CD49f+) and Lin−CD45−Sca-1+CD49f− (Sca-1+) cells, were isolated from adult mice liver by flow cytometry. Robust proliferative activity and clonogenic activity were found in both CD49f+ and Sca-1+ cells through colony-forming tests and cell ...
Source: Journal of Molecular Histology - February 15, 2022 Category: Laboratory Medicine Source Type: research
The synergistic effects of TGF- β1 and RUNX2 on enamel mineralization through regulating ODAPH expression during the maturation stage
In this study,TGF- β1−/−Runx2−/− andTGF- β1+/ −Runx2+/ − mice were successfully generated to clarify the relationship between TGF- β1 and RUNX2 during amelogenesis. Lower mineralization was observed inTGF- β1−/−Runx2−/− andTGF- β1+/ −Runx2+/ − mice than single gene deficient mice. Micro-computed tomography ( μCT) revealed a lower ratio of enamel to dentin density inTGF- β1−/−Runx2−/− mice. Although μCT elucidated a relatively constant enamel thickness, variation was identified by scanning electron microscopy, which revealed thatTGF- β1−/−Runx2−/− mice were more vulnerable to...
Source: Journal of Molecular Histology - February 14, 2022 Category: Laboratory Medicine Source Type: research
Transcriptomic analysis and biological evaluation reveals that LMO3 regulates the osteogenic differentiation of human adipose derived stem cells via PI3K/Akt signaling pathway
In conclusion, based on transcriptomic analysis, we identified key genes involved in regulating the osteogenic differentiation of hADSCs. In addition, we found that LMO3 might act as a positive modulator of hADSC osteogenic differentiation by mediating PI3K/Akt signaling pathway. Manipulating the expression of LMO3 and its associated pathways might contribute to advances in bone regeneration and tissue engineering. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 14, 2022 Category: Laboratory Medicine Source Type: research
Analyses of the circadian clock genes expression in whole embryos and maternal major tissues of mice
AbstractTo create an organism, it is vital to assemble enough cells of the various differentiated types with the correct spatial arrangement within the embryo. Circadian clocks development is closely correlated with all cellular differentiation. However, the expression of its emergence during mammalian development are not fully understood. To determine whether embryonic development is influenced by circadian rhythm, it is necessary to observe the ontogeny of the circadian clock gene. We first measured the expression of key circadian genes in whole embryos and maternal major tissues of 25 female mice using RT-PCR and immuno...
Source: Journal of Molecular Histology - February 11, 2022 Category: Laboratory Medicine Source Type: research
Circ_0120175 promotes laryngeal squamous cell carcinoma development through up-regulating SLC7A11 by sponging miR-330-3p
AbstractThe aim of our study was to illustrate the role of circular RNA 0120175 (circ_0120175) and its associated mechanism in laryngeal squamous cell carcinoma (LSCC) development. The abundance of circ_0120175, microRNA-330-3p (miR-330-3p) and solute carrier family 7, membrane 11 (SLC7A11) messenger RNA and protein was measured by quantitative real time polymerase chain reaction and Western blot assay. Cell proliferation, apoptosis, migration and invasion were assessed by cell counting kit-8 assay, flow cytometry and transwell migration and invasion assays, respectively. The interaction between miR-330-3p and circ_0120175...
Source: Journal of Molecular Histology - February 10, 2022 Category: Laboratory Medicine Source Type: research
Acetyl CoA synthase 2 potentiates ATG5-induced autophagy against neuronal apoptosis after subarachnoid hemorrhage
AbstractATG5-induced autophagy is triggered in the early stages after SAH, which plays a vital role in subarachnoid hemorrhage (SAH). Acyl-CoA synthetase short-chain family 2 (ACSS2) is not just involved in energy metabolism but also binds to TEFB to form a complex translocated to related autophagy genes to regulate the expression of autophagy-related genes. However, the contribution of ACSS2 to the activation of autophagy in early brain injury (EBI) after SAH has barely been discussed. The purpose of this study was to investigate the alterations of ACSS2 and its neuroprotective effects following SAH. We first evaluated th...
Source: Journal of Molecular Histology - February 8, 2022 Category: Laboratory Medicine Source Type: research
Isolation, culture, and identification of ceruminous gland cells
In conclusion, ceruminous glands were successfully isolated, cultured, and expanded from goat EAC skin using the serumcontaining culture system. Importantly, th e isolated glandular cells retained robust proliferation potential and maintained their phenotype and function in early passages (P1–P3), indicating the method’s potential application for ceruminous gland regeneration. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 3, 2022 Category: Laboratory Medicine Source Type: research
