The expression of RIPK3 is associated with cell turnover of gastric mucosa in the mouse and human stomach
AbstractNecroptosis is a novel manner of programmed cell death and important for tissue development, homeostasis, damage, and repair. Activation of receptor-interacting protein kinase 3 (RIPK3), a key member of receptor-interacting protein family in contributing significantly to necroptosis, in tissues is a hallmark of cells dying by necroptosis. However, there are few studies that examine the expression of RIPK3 in the glandular cells of stomachs under physiological condition. We have therefore conducted this study to immunohistochemically characterize the key element of necroptosis, RIPK3, in the mouse and human stomach....
Source: Journal of Molecular Histology - June 25, 2021 Category: Laboratory Medicine Source Type: research
Histological and imunohistochemical alterations of hippocampus and prefrontal cortex in a rat model of Alzheimer like-disease with a preferential role of the flavonoid “hesperidin”
AbstractAlzheimer ’s disease (AD) is a chronic age-related neurodegenerative disease characterized by degeneration of the central cholinergic neurons, inflammation and oxidative stress in the basal forebrain, prefrontal cortex and hippocampus. Hesperidin (Hesp) is one of the flavonoids havinganti-inflammatory and a nti-oxidative properties in some neurodegerative brain lesions. To investigate the possible neuroprotective role of Hespin an AD-like rat model induced experimentally by Scopolamine (Scop). Forty adult male Sprague Dawley rats were randomly allocated into four groups. Group I—(Control), group II— (Hesp) (s...
Source: Journal of Molecular Histology - June 25, 2021 Category: Laboratory Medicine Source Type: research
Circ_PRKDC knockdown promotes skin wound healing by enhancing keratinocyte migration via miR-31/FBN1 axis
AbstractCircular RNA protein kinase, DNA-activated, catalytic subunit (circ_PRKDC) has been found to impede wound healing in diabetic foot ulcers via regulating keratinocyte proliferation and migration. However, the mechanisms underlying circ_PRKDC in skin wound healing remain unclear. The expression of circ_PRKDC, microRNA (miR)-31 and fibrillin 1 (FBN1) was detected using quantitative reverse transcription-polymerase chain reaction and Western blot assays. The migration ability and the changes of matrix metallopeptidase 9 (MMP-9) and MMP2 levels were determined using wound healing, transwell and Western blot assays. The ...
Source: Journal of Molecular Histology - June 18, 2021 Category: Laboratory Medicine Source Type: research
Knockdown of TRIM15 inhibits the activation of hepatic stellate cells
In this study, we screened out 854 differentially expressed genes [Log2 fold change absolute: log2 FC(abs) ≥ 1] in activated LX-2 cells. Subsequently, we performed functional analyses of these differentially expressed genes. Gene Ontology enrichment analysis showed that the target genes were mainly enriched in processes such as positive regulation of cell migration involved in sprouting angiogenes is, negative regulation of keratinocyte proliferation, and nuclear inclusion bodies. Kyoto Encyclopedia of Gene and Genome signaling pathway enrichment analysis revealed that dysregulated genes were involved in signaling p...
Source: Journal of Molecular Histology - June 17, 2021 Category: Laboratory Medicine Source Type: research
Circadian misalignment promotes vascular smooth muscle cell apoptosis via defective autophagy
In conclusion, these findings suggested that the CLOCK-dependent rapamycin signaling pathway is a critical mediator in ox-LDL-induced VSMCs with defective autophagy that exacerbates plaque destabilization. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - June 15, 2021 Category: Laboratory Medicine Source Type: research
Co-expression of transcription factor AP-2beta (TFAP2B) and GATA3 in human mammary epithelial cells with intense, apicobasal immunoreactivity for CK8/18
AbstractAP-2 β is a new mammary epithelial differentiation marker and its expression is preferentially retained and enhanced in lobular carcinoma in situ and invasive lobular breast cancer. In normal breast epithelium AP-2β is expressed in a scattered subpopulation of luminal cells. So far, these cells have no t been further characterized. Co-expression of AP-2β protein and luminal epithelium markers (GATA3, CK8/18), hormone receptors [estrogen receptor (ER), androgen receptor (AR)] and candidate stem cells markers (CK5/14, CD44) were assessed by double-immunofluorescence staining in normal mammary gland epithelium. The...
Source: Journal of Molecular Histology - June 11, 2021 Category: Laboratory Medicine Source Type: research
LncRNA SNHG16 regulates trophoblast functions by the miR-218-5p/LASP1 axis
In conclusion, SNHG16 facilitates trophoblast cell migration and invasion by the miR-218-5p/LASP1 axis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - June 10, 2021 Category: Laboratory Medicine Source Type: research
Circ_0091702 serves as a sponge of miR-545-3p to attenuate sepsis-related acute kidney injury by upregulating THBS2
In conclusion, our results showed thatcirc_0091702 could suppress LPS-induced HK2 cell injury via themiR-545-3p/THBS2 axis, indicating thatcirc_0091702 might be an important biomarker for relieving sepsis-related AKI. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - June 8, 2021 Category: Laboratory Medicine Source Type: research
Selective inhibition of PKC β2 improves Caveolin-3/eNOS signaling and attenuates lipopolysaccharide-induced injury by inhibiting autophagy in H9C2 cardiomyocytes
AbstractLipopolysaccharide (LPS)-induced autophagy is involved in sepsis-associated myocardial injury with increased PKC β2 activation. We previously found hyperglycemia-induced PKCβ2 activation impaired the expression of caveolin-3 (Cav-3), the dominant isoform to form cardiomyocytes caveolae which modulate eNOS signaling to confer cardioprotection in diabetes. However, little is known about the roles of PKCβ2 in autophagy and Cav-3/eNOS signaling in cardiomyocytes during LPS exposure. We hypothesize LPS-induced PKCβ2 activation promotes autophagy and impairs Cav-3/eNOS signaling in LPS-treated cardiomyocytes. H9C2 ca...
Source: Journal of Molecular Histology - June 8, 2021 Category: Laboratory Medicine Source Type: research
Structural and functional relations between the connective tissue and epithelium of enamel organ and their role during enamel maturation
AbstractThe morphological and possible functional interactions between the connective tissue and enamel organ cells were examined during the maturation phase of enamel formation, using immunohistochemical techniques. Decalcified mandibular sections (10 µm) including incisors were used from Wistar rats ages 10–12 weeks. Sections were incubated with one or two primary antibodies targeting cell cytoskeleton (vimentin, α-actin, α-tubulin), dendritic marker (OX6), gap junctions (cx-43), enzymes (nitric-oxide synthase (nos1) and cyclooxygen ase (cox1)), and the ion transporters (Na+/H+ exchanger (NHE1) and Na+/Ca2+ exch...
Source: Journal of Molecular Histology - June 7, 2021 Category: Laboratory Medicine Source Type: research
Fascin promotes the invasion of pituitary adenoma through partial dependence on epithelial –mesenchymal transition
AbstractThe aim of the present study was to investigate the role and potential regulatory mechanisms of fascin in the invasion and epithelial-to-mesenchymal transition of pituitary adenoma cells. A total of 30 specimens were assessed in the present study. The expression levels of fascin in the invasive pituitary adenoma group and non-invasive pituitary adenoma group were determined by immunochemistry. Fascin was downregulated via small interfering RNA in mouse pituitary AtT-20 cells. The proliferation, cell cycle and apoptosis of AtT-20 cells were assessed using Cell Counting Kit ‑8 and flow cytometry. The invasion of At...
Source: Journal of Molecular Histology - June 7, 2021 Category: Laboratory Medicine Source Type: research
LPS induces fibroblast-like synoviocytes RSC-364 cells to pyroptosis through NF- κB mediated dual signalling pathway
AbstractRheumatoid arthritis (RA) is a chronic, progressive, and systemic inflammatory joint disease characterized by synovial inflammation and joint damage. Abnormal activation of fibroblast-like synoviocytes is an initial event of synovial inflammation and joint damage, which can significantly aggravate the progression of RA. Clinical studies have shown that synovitis may be associated with pyroptosis. Therefore, this study is mainly aim for exploring the underlying mechanisms of relationship between inflammation and pyroptosis during synovitis. A cell model of synovitis was constructed by stimulating synovial fibroblast...
Source: Journal of Molecular Histology - June 3, 2021 Category: Laboratory Medicine Source Type: research
Augmented BMP4 signal impairs tongue myogenesis
This study utilized a pathologic model for the syndrome of congenital bony syngnathia,Wnt1-Cre;pMes-Bmp4 mouse line, to explore impacts of enhanced CNCCs-originated BMP4 signal on tongue myogenesis via tissue-tissue interaction. Our results revealed that microglossia, a clinical phenotype of congenital bony syngnathia in humans exhibited inWnt1-Cre;pMes-Bmp4 mice due to impaired myogenesis. The augmented BMP4 signal affected the distal distribution, proliferation, and differentiation of myogenic cells as well as tendon patterning, resulting in disarrangement and atrophy of tongue muscles and the loss of the anterior digast...
Source: Journal of Molecular Histology - June 2, 2021 Category: Laboratory Medicine Source Type: research
The role of leukemia inhibitory factor in pathogenesis of pre-eclampsia: molecular and cell signaling approach
AbstractEndothelial dysfunction is considered as the main hallmark of Preeclampsia (PE). Despite the unknown pathogenesis of PE, different possible causes have been suggested in various studies. In this review, we first studied the Leukemia inhibitory factor (LIF) role in the related pathways to the PE pathogenesis, such as inflammation, endothelial dysfunction and hypertension. LIF can increase the expression of ICAM-1 and VCAM-1 via the JAK/STAT3 pathway, thereby inducing inflammatory responses and endothelial dysfunction. It can also be involved in the vascular vasoconstriction and hypertension by reducing the nitric ox...
Source: Journal of Molecular Histology - June 2, 2021 Category: Laboratory Medicine Source Type: research
Long noncoding HOXA11-AS knockdown suppresses the progression of non-small cell lung cancer by regulating miR-3619-5p/SALL4 axis
AbstractAccumulating evidence suggested that many long noncoding RNAs (lncRNAs) were widely involved in the development and progression of non-small cell lung cancer (NSCLC). However, the roles of lncRNA homeobox A11 antisense (HOXA11-AS) and its underlying mechanism in NSCLC remains largely unknown. The expression levels of HOXA11-AS, miR-3619-5p and sal-like protein 4 (SALL4) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot analysis was used to measure the protein levels of hexokinase II (HK2) and SALL4. Cell proliferation, apoptosis, migration and invasion were determined by 3-(4...
Source: Journal of Molecular Histology - May 29, 2021 Category: Laboratory Medicine Source Type: research
