Combination effects of mesenchymal stem cells transplantation and anodal transcranial direct current stimulation on a cuprizone-induced mouse model of multiple sclerosis
AbstractMultiple sclerosis (MS) has no absolute treatment, and researchers are still exploring to introduce promising therapy for MS. Transcranial direct current stimulation (tDCS), is a safe, non-invasive procedure for brain stimulating which can enhance working memory, cognitive neurohabitation and motor recovery. Here, we evaluated the effects of tDCS treatment and Mesenchymal stem cells (MSCs) transplantation on remyelination ability of a Cuprizone (CPZ)-induced demyelination mouse model. tDCS significantly increased the motor coordination and balance abilities in CPZ  + tDCS and CPZ + tDCS + MSCs mice in c...
Source: Journal of Molecular Histology - August 10, 2022 Category: Laboratory Medicine Source Type: research

AKR1B10 accelerates the production of proinflammatory cytokines via the NF- κB signaling pathway in colon cancer
AbstractAldo –keto reductase family one, member B10 (AKR1B10) has been reported to be involved in the tumorigenesis of various cancers. It has been reported that colorectal cancer is closely associated with chronic inflammation, but the underlying molecular mechanisms are still elusive. In our study, we evalua ted the relationship between AKR1B10 expression and clinicopathological characteristics of colon cancer and showed that AKR1B10 expression was significantly correlated with the T stage and clinical stage of colon cancer. Knockdown of AKR1B10 significantly decreased the expression of the inflammatory cytokines IL1α...
Source: Journal of Molecular Histology - August 3, 2022 Category: Laboratory Medicine Source Type: research

Inventing Engineered Organoids for end-stage liver failure patients
AbstractEnd-stage liver disease (ESLD) is a term used clinically in reference to a group of liver diseases with liver transplantation as the choice of treatment. Due to the limitations of liver transplantation, alternative treatments are needed. The use of primary human hepatocytes represents a valid alternative treatment, but the limitations related to hepatocyte quality, viability, function, conservation, and storage need to be overcome. Transplanted hepatocytes have only been followed for 6 –9 months. Therefore, long-term causes of failures are not yet established, including rejection, apoptosis, or other causes. Othe...
Source: Journal of Molecular Histology - July 27, 2022 Category: Laboratory Medicine Source Type: research

Identification of microRNAs related with neural germ layer lineage-specific progenitors during reprogramming
AbstractDifferentiated cells can be reprogrammed to embryonic stem cell-like cells called induced pluripotent stem cells (iPSCs), in which the natural developmental differentiation process is reversed. It is unclear whether the multi-lineage cells can be isolated and identified during reprogramming. In the current study, we detected the expression of lineage markers, isolated neural lineages, and identified the related microRNAs during iPSC formation. Our results demonstrated that a neuroectoderm appeared earlier than mesoderm and definitive endoderm before forming colonies when mouse embryonic fibroblasts were subjected t...
Source: Journal of Molecular Histology - July 23, 2022 Category: Laboratory Medicine Source Type: research

Correction to: MircoRNA-126-5p inhibits apoptosis of endothelial cell in vascular arterial walls via NF- κB/PI3K/AKT/mTOR signaling pathway in atherosclerosis
(Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - July 23, 2022 Category: Laboratory Medicine Source Type: research

Recombinant jurkat cells (HMGN2-T cells) secrete cytokines and inhibit the growth of tumor cells
In conclusion, the HMGN2-T cells were well-established and have the effect of secreting cytokines and killing tumor cells. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - July 21, 2022 Category: Laboratory Medicine Source Type: research

Isoliquiritigenin attenuates pathological cardiac hypertrophy via regulating AMPK α in vivo and in vitro
AbstractIsoliquiritigenin (ISL) is a type of flavonoid, derived from the root of the legume plantGlycyrrhiza, that has multiple pharmacological properties. However, its role in cardiac remodeling induced by pressure overload has yet to be fully elucidated. Aortic banding (AB) surgery was used to establish a cardiac hypertrophy model in male C57BL/6 mice. Mice were randomly divided into four groups (n  = 20 per group) as follows: Sham + vehicle, sham + ISL, AB + vehicle and AB + ISL. ISL was administered to the mice intragastrically for 1 week after the operation. To evaluate the role of ISL in mice cha...
Source: Journal of Molecular Histology - July 14, 2022 Category: Laboratory Medicine Source Type: research

Calenduloside E alleviates cerebral ischemia/reperfusion injury by preserving mitochondrial function
In this study, we established an oxygen-glucose deprivation/reoxygenation (OGD/R) model in HT22 cells. We found that CE significantly attenuated the OGD/R-induced inhibition of cell viability and apoptotic cell death in HT22 cells. Moreover, CE treatment significantly ameliorated OGD/R-induced mitochondrial fission by inhibiting mitochondrial dynamin-related protein 1 (Drp1) recruitment and increasing Drp1 phosphorylation at Ser637. CE treatment significantly ameliorated OGD/R-induced mitochondrial dysfunction by increasing the mitochondrial membrane potential and reducing the mitochondrial ROS and cellular calcium accumul...
Source: Journal of Molecular Histology - July 12, 2022 Category: Laboratory Medicine Source Type: research

MiR-135b improves proliferation and regulates chemotherapy resistance in ovarian cancer
AbstractMicroRNAs act as regulators in ovarian tumorigenesis and progression by involving different molecular pathways. Here, we examined the role of miR-135b on growth, chemotherapy resistance in OVCAR3 and SKOV3 ovarian cancer cells. MTT assay was performed to examine proliferation. Transwell migration and matrigel invasion assays were used to assess migration and invasion. Caspase-Glo3/7 assay was carried out to evaluate apoptosis. The dual-luciferase reporter assay was performed to validate the putative binding site. Meanwhile, the miR-135b levels in human ovarian cancer tissue were detected by qPCR assay. Overexpressi...
Source: Journal of Molecular Histology - July 6, 2022 Category: Laboratory Medicine Source Type: research

Ginsenoside Rb3 attenuates skin flap ischemia-reperfusion damage by inhibiting STING-IRF3 signaling
AbstractWe investigate the protective effect of ginsenoside Rb3 on skin flap microvasculature following ischemia-reperfusion (I/R) injury and its regulatory mechanism. We used a rat model of I/R injury with the right iliolumbar artery and oxidative stress model of human dermal microvascular endothelial cells. The effects of Rb3 on skin flap tissue and endothelial cell survival, STING-IRF3 pathway activation, and endothelial cell adhesion were measured. Following reperfusion, the survival rate of rat perforator flaps in the Rb3-treated group gradually increased with increasing Rb3 concentration. The treatment also reduced t...
Source: Journal of Molecular Histology - June 22, 2022 Category: Laboratory Medicine Source Type: research

Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway
AbstractCisplatin, a first-line chemotherapeutic agent commonly used to treat various solid tumors, induce severe adverse effects, especially nephrotoxicity, which largely limits its clinical application. However, the currently used measures to prevent nephrotoxicity are not ideal owing to the mechanisms underlying cisplatin-induced nephrotoxicity are not comprehensively understood. Herein, we examined the effects of silibinin on cisplatin-induced nephrotoxicity and found that silibinin exerted cytoprotection effects during cisplatin treatment in HEK293 cells and in a cisplatin-induced acute kidney injury (AKI) model. Mech...
Source: Journal of Molecular Histology - June 21, 2022 Category: Laboratory Medicine Source Type: research

CRIF1 promotes the progression of non-small-cell lung cancer by SIRT3- mediated deacetylation of PYCR1
In this study, the specific role and related mechanism of CRIF1 in non-small cell lung cancer (NSCLC) were explored in this research. TheRT-PCR, western blot and IHC assays were used to examine the expression level of CRIF1 in NSCLC tissue, tissue adjacent to carcinoma, NSCLC cell lines and human normal lung epithelial cells. Next, colony formation assay, Alamar blue Kit and EdU assays were employed to examine the proliferation of transfected A549 and NCI-H2009 cells. Measurement of mitochondrial permeability transition pore opening, ATP production and cellular oxygen consumption were used to evaluate the mitochondrial apo...
Source: Journal of Molecular Histology - June 18, 2022 Category: Laboratory Medicine Source Type: research

JAK-STAT signaling mediates the senescence of cartilage-derived stem/progenitor cells
In conclusion, our results demonstrated the critical role of JAK-STAT signaling pathway in CSPCs senescence. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - June 18, 2022 Category: Laboratory Medicine Source Type: research

β2-adrenergic receptor drives the metastasis and invasion of pancreatic ductal adenocarcinoma through activating Cdc42 signaling pathway
AbstractRecent investigations indicate that β2-adrenergic receptor (β2-AR) signaling may facilitate the progression of various tumors, whose underlying mechanisms remain largely elusive. In the present study, we showed that β2-AR recruited Cdc42 in response to isoproterenol (ISO, a β-AR selective agonist) exposure in pancreatic ductal ade nocarcinoma (PDAC) cells. The association of β2-AR and Cdc42 promoted the activation of Cdc42, as revealed by increased levels of Cdc42-GTP, and co-incubation with β2-AR antagonist abrogated ISO-induced activation of Cdc42. β2-AR-mediated Cdc42 activation further led to the phospho...
Source: Journal of Molecular Histology - June 18, 2022 Category: Laboratory Medicine Source Type: research

TGF- β1 receptor blockade attenuates unilateral ureteral obstruction-induced renal fibrosis in C57BL/6 mice through attenuating Smad and MAPK pathways
In conclusion, we demonstrated that TGF-β1 receptor blockade can prevent UUO-induced renal fibrosis through indirect modulation of Smad and MAPKs signaling pathways and may be useful as a therapeutic agent in treatment and/or prevention of renal fibrosis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - June 15, 2022 Category: Laboratory Medicine Source Type: research