YTHDF2 negatively correlates with tumor immune infiltration in small cell lung cancer
This study aimed to explore the role of YTHDF2 in small cell lung cancer (SCLC) by collecting 20 SCLC patients from our hospital (cohort 1) and 48 Chinese SCLC patients from the GEO database (cohort 2). We evaluated the prognostic value of YTHDF2 using Kaplan-Meier survival analysis, Log-rank test, and Cox regression analysis. Additionally, we employed Gene Set Enrichment Analysis (GSEA) to screen different signaling pathways. We also investigated the correlation between the expression of m6A-related genes and SCLC molecular subtype and tumor immune microenvironment (TIME). Furthermore, we utilized multiplex immunofluoresc...
Source: Journal of Molecular Histology - June 26, 2023 Category: Laboratory Medicine Source Type: research
Expressions of Notch signalling pathway members during early pregnancy in mice
AbstractAlthough pregnancy is initiated and maintained through highly complex mechanisms, it is essential to understand the events that occur before and during early pregnancy to understand a healthy implantation process. The Notch signal, thought to be involved in this process, is frequently the subject of research with its different aspects. To better understand the role of Notch signaling in the peri-implantation period of the mouse uterus, we investigated the state of expression and localization of Notch 3, Notch 4, Rbp-J, Hes1, Hes7, Hey2, HeyL, and Fbw7 in the uterus and implantation sites in early pregnancy. Balb/C ...
Source: Journal of Molecular Histology - June 21, 2023 Category: Laboratory Medicine Source Type: research
Knockdown of USF2 inhibits pyroptosis of podocytes and attenuates kidney injury in lupus nephritis
AbstractAs an essential factor in the prognosis of Systemic lupus erythematosus (SLE), lupus nephritis (LN) can accelerate the rate at which patients with SLE can transition to chronic kidney disease or even end-stage renal disease (ESRD). Proteinuria due to decreased glomerular filtration rate following podocyte injury is LN ’s most common clinical manifestation. Podocyte pyroptosis and related inflammatory factors in its process can promote lupus to involve kidney cells and worsen the occurrence and progression of LN, but its regulatory mechanism remains unknown. Accumulating evidence has shown that upstream stimulat o...
Source: Journal of Molecular Histology - June 21, 2023 Category: Laboratory Medicine Source Type: research
Lobe-specific responses of TRAMP mice dorsolateral prostate following celecoxib and nintedanib therapy
AbstractDelayed cancer progression in the ventral prostate of the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model has been previously reported upon celecoxib and nintedanib co-administration. Herein, we sought to further investigate the effects of these drugs association in some of their direct molecular targets (COX-2, VEGF and VEGFR-2) and in reactive stroma markers (TGF- β, αSMA, vimentin and pro-collagen 1) in the dorsolateral prostate, looking for lobe-specific responses. Male TRAMP mice were treated with celecoxib (10 mg/Kg, i.o.) and/or nintedanib (15 mg/Kg, i.o.) for 6 weeks and prostate was harv...
Source: Journal of Molecular Histology - June 19, 2023 Category: Laboratory Medicine Source Type: research
CORM-3 alleviates the intestinal injury in a rodent model of hemorrhage shock and resuscitation: roles of GFAP-positive glia
AbstractHemorrhagic shock and resuscitation (HSR) can induce severe intestinal damages, thereby leading to sepsis and long-term complications including dysbacteriosis and pulmonary injury. The NOD-like receptor protein 3 (NLRP3) inflammasome facilitates inflammation-associated cell recruitment in the gastrointestinal tract, and participates in many inflammatory bowel diseases. Previous studies have shown that exogenous carbon monoxide (CO) exerts neuroprotective effects against pyroptosis after HSR. We aimed to investigate whether carbon monoxide-releasing molecules-3 (CORM-3), an exogenous CO compound, could attenuate HSR...
Source: Journal of Molecular Histology - June 19, 2023 Category: Laboratory Medicine Source Type: research
EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage
In conclusion, genistein can induce a transactivation of EGFR leading to downstream cell signaling pathways as early events associated with regeneration and hepatoprotection following subacute liver damage. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 25, 2023 Category: Laboratory Medicine Source Type: research
NeuN distribution in brain structures of normal and Zika-infected suckling mice
AbstractMicrocephaly is the more severe brain malformation because of Zika virus infection. Increased vulnerability of neural stem and progenitor cells to Zika infection during prenatal neurodevelopment impairs the complete formation of cortical layers. Normal development of cerebellum is also affected. However, the follow-up of apparently healthy children born to Zika exposed mothers during pregnancy has revealed other neurological sequelae. This suggests Zika infection susceptibility remains in nervous tissue after neurogenesis end, when differentiated neuronal populations predominate. The neuronal nuclear protein (NeuN)...
Source: Journal of Molecular Histology - May 18, 2023 Category: Laboratory Medicine Source Type: research
Lack of Rab27a attenuates foam cell formation and macrophage inflammation in uremic apolipoprotein E knockout mice
In conclusion, our study indicated thatRab27a deficiency attenuated foam cell formation and macrophage inflammation, depending on the NF- κB pathway activation, to inhibit AS progression in uremicapoE−/− mice. This finding may provide a new targeting strategy for UAAS therapy. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 11, 2023 Category: Laboratory Medicine Source Type: research
Circadian regulation of mTORC1 signaling via Per2 dependent mechanism disrupts folliculogenesis and oocyte maturation in female mice
In conclusion, these results suggest that the circadian clock may control ovarian aging by regulating mTOR signaling pathway through Per2 expression. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 10, 2023 Category: Laboratory Medicine Source Type: research
Administration of necrostatin-1 ameliorates glucocorticoid-induced osteonecrosis of the femoral head in rats
In this study, rat models of GC-induced ONFH were established to evaluate the effects of necrostatin-1 on osteonecrotic changes and repair processes. Osteonecrosis was verified by histopathological staining. An analysis of trabecular bone architecture was performed to evaluate osteogenesis in the osteonecrotic zone. Then, necroptotic signaling molecules such as RIP1 and RIP3 were examined by immunohistochemistry. Histopathological observations indicated that necrostatin-1 administration reduced the incidence of osteonecrosis and the osteogenic response in subchondral areas. Additionally, bone histomorphometry demonstrated ...
Source: Journal of Molecular Histology - May 9, 2023 Category: Laboratory Medicine Source Type: research
The role of CNPY2 in endothelial injury and inflammation during the progress of atherosclerosis
In conclusion, this study indicated that high level of CNPY2 induces VECs injury by activating PERK signaling and thus participating in the progress of atherosclerosis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - April 27, 2023 Category: Laboratory Medicine Source Type: research
Dexmedetomidine alleviates cerebral ischemia-reperfusion injury via inhibiting autophagy through PI3K/Akt/mTOR pathway
AbstractDexmedetomidine has been shown to protect against cerebral ischemia-reperfusion injury (CIRI). Nevertheless, the precise mechanism is obscure. In order to explore the effect of dexmedetomidine pre-conditioning on autophagy against CIRI in rats, middle cerebral artery occlusion (MCAO) was conducted to establish cerebral ischemia-reperfusion (I/R) model in male SD rats with 2 h ischemia and 24 h reperfusion. Dexmedetomidine was delivered to rats at 10, 50 and 100 µg/kg doses respectively, and LY294002, a PI3K/Akt/mTOR pathway inhibitor, was administered at 10 mg/kg intraperitoneally 30 min before MCAO. Neurolo...
Source: Journal of Molecular Histology - April 26, 2023 Category: Laboratory Medicine Source Type: research
Correction to: Possible healing effects of Salvadora persica extract (MISWAK) and laser therapy in a rabbit model of a caustic-induced tongue ulcers: histological, immunohistochemical and biochemical study
(Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - April 13, 2023 Category: Laboratory Medicine Source Type: research
Comparative toxicological evaluations of novel forms nano-pesticides in liver and lung of albino rats
In conclusion, CuONSp indu ced biological alternation in liver and lung more than CuONF. So, CuONF is less toxic compared to CuONSp when used as nano-pesticide in agricultural applications.Graphical abstract (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 31, 2023 Category: Laboratory Medicine Source Type: research
C1orf109 promotes malignant phenotype of liver cancer via wnt signaling pathway in a CK2-dependent manner
In this study, both bioinformatics and immunohistochemical staining revealed that C1orf109 was overexpressed in the cytoplasm of liver cancer cells, and the positive ratio of C1orf109 in liver cancer samples (42.5%, 37/87) was significantly higher than that in normal liver tissues (10%, 3/30, P = 0.0012). C1orf109 expression was correlated with an advanced TNM stage (P = 0.017) and vascular invasion (P = 0.023) and predicted the poor overall survival of patients with liver cancer (P = 0.001). C1orf109 facilitated tumor growth, colony formation, migration, and invasion by a ctivating Wnt signaling by upregu...
Source: Journal of Molecular Histology - March 29, 2023 Category: Laboratory Medicine Source Type: research
