Elevated miR-129-5p attenuates hepatic fibrosis through the NF- κB signaling pathway via PEG3 in a carbon CCl 4 rat model
This study is performed to decipher, potential of miR-129-5p to influence the progression of hepatic fibrosis in a carbon tetrachloride (CCl4) rat model. Rat hepatic fibrosis was successfully established by subcutaneous injection of 50% CCl4. RT-qPCR revealed that miR-129-5p was poorly expressed and PEG3 was highly expressed in hepatic fibrosis tissues. As reflected by dual-luciferase reporter gene assay, miR-129-5p targeted and reduced the expression of PEG3. Thereafter, miR-129-5p antagomir or short hairpin RNA against paternally expressed gene 3 (PEG3) was adopted for gain- and loss-of-function assay to determine the mo...
Source: Journal of Molecular Histology - March 20, 2021 Category: Laboratory Medicine Source Type: research
Upregulation of CPNE3 suppresses invasion, migration and proliferation of glioblastoma cells through FAK pathway inactivation
This study characterizes CPNE3 expression in GBM along with its impact and underlying molecular mechanism with regards to cellular migration, invasion and proliferation. Immunohistochemistry was used to characterizes CPNE3 expression in the glioma tissues. Then, knockdown of CPNE3 expression was used to analyze the role of CPNE3 in GBM cell viability, migration, invasion. Western blot analysis was performed to measure the protein levels of FAK signaling pathway. We found that GBM tissues had higher CPNE3 expressions as compared to those in normal brain tissues. CPNE3 silencing in GBM cells impaired the migratory, invasive ...
Source: Journal of Molecular Histology - March 16, 2021 Category: Laboratory Medicine Source Type: research
LINC00152 mediates CD8 + T-cell infiltration in gastric cancer through binding to EZH2 and regulating the CXCL9, 10/CXCR3 axis
This study aimed to annotate the role of long intergenic non-coding RNA 152 (LINC00152) in CD8+ T cells mediated immune responses in gastric cancer (GC) and the underlying mechanism. LINC00152 expression levels were detected through RT-PCR. For tumor engraftment, HGC-27 cells that received LINC00152 shRNA, LINC00152 overexpression vectors, enhancer of zeste homolog 2 (EZH2) shRNA or combination transfection were injected into mice. Chromatin immunoprecipitation (ChIP) assay was used to explore the interaction between LINC00152, Cys-X-cys ligand 9 (CXCL9) and Cys-X-cys ligand 10 (CXCL10). Flow cytometry was adopted to measu...
Source: Journal of Molecular Histology - March 11, 2021 Category: Laboratory Medicine Source Type: research
Constitutive activation of β-catenin in odontoblasts induces aberrant pulp calcification in mouse incisors
In this study, we explored whether β-catenin participated in odontoblast polarization t o regulate the secretion of mineralization proteins. We established Col1-CreErt2; β-catenin exon3fl/fl (CA- β-catenin) mice, which constitutively activate β-catenin in odontoblasts. CA-β-catenin mice exhibited disorganization and depolarization of incisor odontoblasts. Moreover, the incisor dentin was hypomineralized, and ectopic calcification was found in mouse incisor pulp. In addition, by constituti ve activation of β-catenin, the expression levels of the core polarity molecule Cdc42 and its downstream polarity protein complex ...
Source: Journal of Molecular Histology - March 10, 2021 Category: Laboratory Medicine Source Type: research
LncRNA TRHDE-AS1 inhibit the scar fibroblasts proliferation via miR-181a-5p/PTEN axis
In conclusion, our study suggested that lncRNA TRHDE-AS1/miR-181a-5p/PTEN axis plays an important role in promoting hypertrophic scar formation, which may be effectively used as a therapeutic target for hypertrophic scar treatment. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 6, 2021 Category: Laboratory Medicine Source Type: research
miR-495 reduces neuronal cell apoptosis and relieves acute spinal cord injury through inhibiting PRDM5
This study aims to investigate the role of miR-495 in neuronal cell apoptosis after acute spinal cord injury (ASCI). The ASCI rat model was established and the Basso, Beattie, and Bresnahan (BBB) score was assessed. miR-495, PR domain containing 5 (PRDM5), and Bcl-2 expressions were measured by qRT-PCR or western blotting. Neuronal cell line PC-12 was subjected to hypoxia condition to simulate the in vitro ASCI model. PC-12 cell apoptosis was measured by flow cytometry, and the interaction between miR-495 and PRDM5 was confirmed by dual luciferase reporter assay. Results showed that BBB score was significantly decreased in...
Source: Journal of Molecular Histology - February 25, 2021 Category: Laboratory Medicine Source Type: research
SR-B1 and CD10 combined immunoprofile for differential diagnosis of metastatic clear cell renal cell carcinoma and clear cell carcinoma of the ovary
Conclusions: our findings demonstrate that the combination of SR-B1 and CD10 immunoprofiling is a valuab le tool for differential diagnosis of ccRCC and CCOC. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 19, 2021 Category: Laboratory Medicine Source Type: research
cIAP2 expression and clinical significance in pigmented villonodular synovitis
This study suggested that cIAP2 overexpression plays an important role in the anti-apoptotic, proliferative and invasive growth of PVNS, which may account for the recurrence and poor prognosis of DPVNS. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 18, 2021 Category: Laboratory Medicine Source Type: research
Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair
AbstractSonic hedgehog (Shh) and Indian hedgehog (Ihh) have been shown to control the induction of early cartilaginous differentiation. However, it is unclear whether Ihh and Shh exert synergistic effects on chondrogenesis during articular cartilage repair. Herein, we investigate the effects of chondrogenesis of bone-derived mesenchymal stem cells (BMSCs) following co-transfection with Shh and Ihh via adenoviral vectors in vitro and in vivo. A rotary cell culture system (RCCS) and Cytodex 3 microcarriers were used to create a stereoscopic dynamic environment for cell culture. In the RCCS environment, BMSCs co-transfected w...
Source: Journal of Molecular Histology - February 17, 2021 Category: Laboratory Medicine Source Type: research
Semaphorin 3A promotes the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells in inflammatory environments by suppressing the Wnt/ β-catenin signaling pathway
In conclusion, Sema3A could protect BMSCs from LPS-mediated inhibition of osteogenic differentiation in inflammatory environments by suppressing the Wnt/β-catenin pathway. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 10, 2021 Category: Laboratory Medicine Source Type: research
LGR4 silence aggravates ischemic injury by modulating mitochondrial function and oxidative stress via ERK signaling pathway in H9c2 cells
AbstractIt is reported that LGR4 (leucine-rich repeat domain containing G protein-coupled receptor 4) plays a crucial role in the physiological function of many organs. However, few data are available on the function and mechanism of LGR4 in myocardial ischemia –reperfusion (I/R) injury. The aim of this study was to explore the function and mechanism of LGR4 in I/R injury. We incubated H9c2 cells in simulating ischemia buffer and then re-incubated them in normal culture medium to establish a model of I/R injury in vitro. The expression of LGR4 was evalua ted by RT-PCR and western blot. Besides, the cell apoptosis was eva...
Source: Journal of Molecular Histology - February 9, 2021 Category: Laboratory Medicine Source Type: research
Nogo-A- Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling
AbstractNogo-A protein consists of two main extracellular domains: Nogo-66 (rat amino acid [aa] 1019 –1083) and Nogo-A-Δ20 (extracellular, active 180 amino acid Nogo-A region), which serve as strong inhibitors of axon regeneration in the adult CNS (Central Nervous System). Although receptors S1PR2 and HSPGs have been identified as Nogo-A-Δ20 binding proteins, it remains at present elusive wheth er other receptors directly interacting with Nogo-A-Δ20 exist, and decrease cell death. On the other hand, the key roles of EphA4 in the regulation of glioblastoma, axon regeneration and NSCs (Neural Stem Cells) proliferation o...
Source: Journal of Molecular Histology - February 8, 2021 Category: Laboratory Medicine Source Type: research
Exosomes with high level of miR-181c from bone marrow-derived mesenchymal stem cells inhibit inflammation and apoptosis to alleviate spinal cord injury
In conclusion, we verifi ed that BMSCs-exo could protect against SCI through its content microRNA-181c which suppressed the inflammation in microglia and spinal cord. It was related to the inhibition of PTEN and the suppression of NF-κB signal, and finally decreasing inflammation and apoptosis in spinal cord and improved S CI. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 6, 2021 Category: Laboratory Medicine Source Type: research
Over-expression of Fgf8 in cardiac neural crest cells leads to persistent truncus arteriosus
In this study,Rosa26R-Fgf8 knock-in allele was constitutively activated byWnt1-cre transgene in the mouse neural crest cells presumptive for the endocardial cushion of outflow tract. Surprisingly,Wnt1-cre; Rosa26R-Fgf8 mouse embryos exhibited persistent truncus arteriosus and died prior to E15.5. The cardiac neural crest cells inWnt1-cre; Rosa26R-Fgf8 truncus arteriosus did not degenerate as in WT controls, but proliferated into a thickened endocardial cushion and then, blocked the blood outflow from cardiac chambers into the lungs, which resulted in the embryonic lethality. Although the spiral aorticopulmonary septum fail...
Source: Journal of Molecular Histology - February 5, 2021 Category: Laboratory Medicine Source Type: research
miR-520d suppresses rapid pacing-induced apoptosis of atrial myocytes through mediation of ADAM10
In this study, the atrial myocytes grew adherently with irregular morphology. Immunofluorescence showed that more than 90% of atrial myocytes were α-sarcomeric actin (α-SCA) positive, indicating that the primary cells were positive for α-SCA staining and atrial myocytes were successfully isolated. The pacing atrial myocyte model was established after rapid pacing stimulation and we found the rapid pacing stimulation caused elevated ADAM10 a nd suppressed miR-520d. CCK-8 assay was applied for evaluation of cell viability, TUNEL staining for assessment of cell apoptosis and dual-luciferase reporter gene assay for verifica...
Source: Journal of Molecular Histology - February 5, 2021 Category: Laboratory Medicine Source Type: research
