TGF- β2-induced circ-PRDM5 regulates migration, invasion, and EMT through the miR-92b-3p/COL1A2 pathway in human lens epithelial cells

AbstractCircRNAcirc-PRDM5 (PR/SET domain 5) (circ-PRDM5) is overexpressed in age-related cataracts. Nevertheless, the biological role of circ-PRDM5 in posterior capsule opacities (PCO) (a common complication after cataract surgery) is unclear. Human lens epithelial cells SRA01/04 (LECs) were stimulated with TGF- β2 (transforming growth factor beta-2) to mimic the PCO model in vitro. Cell viability, migration, and invasion were determined by MTT, transwell, or wound-healing assays. Protein levels of EMT (epithelial-to-mesenchymal transition) markers andCOL1A2 (collagen type I alpha 2 chain) were analyzed by western blotting (WB). Relative expression ofcirc-PRDM5,miR-92b-3p, andCOL1A2 mRNA was analyzed by qRT-PCR. The targeting relationship was confirmed by dual-luciferase reporter and RIP assays. We observed thatcirc-PRDM5 andCOL1A2 were upregulated in PCO tissues and TGF- β2-treated LECs, whilemiR-92b-3p was downregulated. Bothcirc-PRDM5 andCOL1A2 knockdown impaired TGF- β2-induced LEC migration, invasion, and EMT. Also,circ-PRDM5 could adsorbmiR-92b-3p to regulateCOL1A2 expression. Furthermore,miR-92b-3p inhibitor offsetcirc-PRDM5 knockdown-mediated influence on migration, invasion, and EMT of LECs under TGF- β2 stimulation. Also,COL1A2 overexpression overturned the repressive influence ofmiR-92b-3p mimic on TGF- β2-induced LEC migration, invasion, and EMT. In summary, TGF-β2-induced circ-PRDM5 facilitated LEC migration, invasion, and EMT by adsorbing miR-92b-3p and i...
Source: Journal of Molecular Histology - Category: Laboratory Medicine Source Type: research