Journal of Molecular and Cellular Cardiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Partial and complete loss of myosin binding protein H-like cause cardiac conduction defects
A premature truncation of MYBPHL in humans and a loss of Mybphl in mice is associated with dilated cardiomyopathy, atrial and ventricular arrhythmias, and atrial enlargement. MYBPHL encodes myosin binding protein H-like (MyBP-HL). Prior work in mice indirectly identified Mybphl expression in the atria and in small puncta throughout the ventricle. Because of its genetic association with human and mouse cardiac conduction system disease, we evaluated the anatomical localization of MyBP-HL and the consequences of loss of MyBP-HL on conduction system function. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - May 6, 2022 Category: Cytology Authors: David Y. Barefield, Sean Yamakawa, Ibrahim Tahtah, Jordan J. Sell, Michael Broman, Brigitte Laforest, Sloane Harris, Alejandro A. Alvarez, Kelly N. Araujo, Megan J. Puckelwartz, J. Andrew Wasserstrom, Glenn I. Fishman, Elizabeth M. McNally Source Type: research

The cell-autonomous and non –cell-autonomous roles of the Hippo pathway in heart regeneration
Cardiomyocytes are differentiated heart muscle cells with minimal self-renewal ability. Thus, loss of cardiomyocytes from cardiovascular disease and injury cannot be effectively replenished. Recent studies in animal models have indicated that induction of endogenous cardiomyocyte proliferation is essential for cardiac renewal and that inhibiting the Hippo signaling pathway can stimulate cardiomyocyte proliferation and heart regeneration. Increasing evidence has suggested that cardiomyocyte proliferation requires a permissive microenvironment that consists of multiple cell types. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - May 5, 2022 Category: Cytology Authors: Shijie Liu, Rich Gang Li, James F. Martin Source Type: research

Minimally invasive delivery of a hydrogel-based exosome patch to prevent heart failure
Coronary heart disease (CHD) has been the number one killer in the United States for decades and causes millions of deaths each year. Clinical treatment of heart ischemic injury relieves symptoms in the acute stage of CHD; however, patients with an infarcted heart muscle can develop heart failure (HF) due to chronic maladaptive remodeling. Regenerative therapy has been studied as a potential treatment option for myocardial infarction (MI) and HF. Cardiac patches have been designed and tested to increase therapeutic retention and integration in this field. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - May 3, 2022 Category: Cytology Authors: George Cheng, Dashuai Zhu, Ke Huang, Thomas G. Caranasos Source Type: research

PPAR- δ: A key nuclear receptor in vascular function and remodeling
Vascular function is critical for the maintenance of body's homeostasis and is tightly regulated by complex interactions among the vessel wall, hemodynamics, neuro-endocrine factors and metabolic alteration. A variety of cardiovascular risks instigate pro-inflammatory and oxidative responses to impair vascular function, leading to pathological vascular remodeling. Peroxisome proliferator-activated receptor- δ (PPAR-δ) is a ligand-activated nuclear receptor and transcription factor that regulates cell growth and differentiation, metabolism and wound healing. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 28, 2022 Category: Cytology Authors: Lei Xiao, Nanping Wang Source Type: research

An advanced endothelial murine HFpEF model: eNOS is critical for angiotensin 1 –7 rescue of the diabetic phenotype
Diabetes and obesity are hallmark clinical features of patients with heart failure with preserved ejection fraction (HFpEF). [1,2] Activation of the renin-angiotensin system (RAS) can exacerbate HFpEF and is a primary cause of cardiorenal and vascular injury in patients living with different forms of metabolic syndrome. [1,2] Heightened Ang II signaling drives cardiorenal maladaptation in diabetic pathological states. [1,3,4] Angiotensin-converting enzyme 2 (ACE2) metabolizes Ang II into Ang 1 –7, offering an endogenous counterregulatory RAS axis which is frequently dysregulated in diseased states. (Source: Journal of Mo...
Source: Journal of Molecular and Cellular Cardiology - April 27, 2022 Category: Cytology Authors: Mahmoud Gheblawi, Amanda A. de Oliveira, Vanessa R. Williams, Rohan John, Maria B. Grant, James W. Scholey, Gavin Y. Oudit Tags: Letter to the editor Source Type: research

Up next: The dawn of systems biology in HFpEF research
Heart failure with preserved ejection fraction (HFpEF) is a complex clinical syndrome with heterogeneous pathophysiology and presentation [1]. Despite this, on tissue and cellular level, common pathogenetic substrates have emerged as critical in the development and progression of HFpEF: e.g., systemic (meta)-inflammation, abnormal myocardial energetics, tissue fibrosis, vascular dysfunction and dysregulated signaling pathways in cardiomyocytes [2,3]. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 27, 2022 Category: Cytology Authors: David Bode, Cristian Sotomayor-Flores, Gabriele G. Schiattarella Tags: Letter to the editor Source Type: research

Time series RNA-seq analysis identifies MAPK10 as a critical gene in diabetes mellitus-induced atrial fibrillation in mice
Atrial fibrillation (AF) is a major complication of type 2 diabetes mellitus (T2DM) and plays critical roles in the pathogenesis of atrial remodeling. However, the differentially expressed genes in atria during the development of AF induced by hyperglycemia have rarely been reported. Here, we showed time-dependent increased AF incidence and duration, atrial enlargement, inflammation, fibrosis, conduction time and action potential duration in db/db mice, a model of T2DM. RNA sequencing analysis showed that 2256 genes were differentially expressed in the atria at 12, 14 and 16  weeks. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 27, 2022 Category: Cytology Authors: Fei Liu, Yawen Deng, Yong Zhao, Zhitong Li, Jinghan Gao, Yunlong Zhang, Xiaolei Yang, Yang Liu, Yunlong Xia Source Type: research

E-cigarette exposure with or without heating the e-liquid induces differential remodeling in the lungs and right heart of mice
This study investigated the differential adverse effects of heating-associated by-products versus the intact components of EC aerosol to the lungs and heart of mice. We further dissected the roles of caspase recruitment domain-containing protein 9 (CARD9)-associated innate immune response and NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome in EC exposure-induced cardiopulmonary injury. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 27, 2022 Category: Cytology Authors: Yohannes Getiye, Matthew R. Peterson, Brandon D. Phillips, Daniel Carrillo, Bledar Bisha, Guanglong He Source Type: research

The effects of xeno-free cryopreservation on the contractile properties of human iPSC derived cardiomyocytes
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have advanced our ability to study the basic function of the heart and model cardiac diseases. Due to the complexities in stem cell culture and differentiation protocols, many researchers source their hiPSC-CMs from collaborators or commercial biobanks. Generally, the field has assumed the health of frozen cardiomyocytes is unchanged if the cells adhere to the substrate and commence beating. However, very few have investigated the effects of cryopreservation on hiPSC-CM's functional and transcriptional health at the cellular and molecular level. (Source...
Source: Journal of Molecular and Cellular Cardiology - April 20, 2022 Category: Cytology Authors: Orlando Chirikian, Sam Feinstein, Mohamed A. Faynus, Anna A. Kim, Kerry Lane, Gabriela Torres, Jeffrey Pham, Zachary Singh, Amanda Nguyen, Dilip Thomas, Dennis O. Clegg, Joseph C. Wu, Beth L. Pruitt Source Type: research

Metabolic therapy for managing heart failure with preserved ejection fraction
The global increase in average life expectancy and the pandemics of obesity, diabetes, and hypertension, have pushed the prevalence of heart failure with preserved fraction (HFpEF) to the edge of socioeconomic sustainability [1]. Sedentary lifestyle and obesogenic diets are major drivers of metabolic disorders and multimorbidities, which exacerbate one another and promote age-related diastolic dysfunction, leading to HFpEF through multiple, albeit poorly characterized, signaling pathways and mechanisms [2]. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 20, 2022 Category: Cytology Authors: Simon Sedej, Mahmoud Abdellatif Tags: Letter to the editor Source Type: research

Targeting endothelial dysfunction and inflammation
Vascular endothelium maintains vascular homeostasis through liberating a spectrum of vasoactive molecules, both protective and harmful regulators of vascular tone, structural remodeling, inflammation and atherogenesis. An intricate balance between endothelium-derived relaxing factors (nitric oxide, prostacyclin and endothelium-derived hyperpolarizing factor) and endothelium-derived contracting factors (superoxide anion, endothelin-1 and constrictive prostaglandins) tightly regulates vascular function. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 20, 2022 Category: Cytology Authors: Li Wang, Chak Kwong Cheng, Min Yi, Kathy O. Lui, Yu Huang Source Type: research

Obscurin regulates ankyrin macromolecular complex formation
In this study, we demonstrate that two different ankyrins interact with obscurin's carboxyl terminus via independent ankyrin-binding domains (ABDs). Using in-vitro binding assays, co-precipitation assays, and FLIM-FRET analysis, we show that obscurin interacts with small ankyrin 1.5 (sAnk1.5) and the muscle-specific ankyrin-G isoform (AnkG107). (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 18, 2022 Category: Cytology Authors: Janani Subramaniam, Gokay Yamankurt, Shane R. Cunha Source Type: research

Methods of mouse cardiomyocyte isolation from postnatal heart
Primary cardiomyocytes (CMs) are invaluable materials used to study in vitro cardiac physiology and pathophysiology. However, there is still a lack of comprehensive, systematic and comparative studies on methods for isolation of primary CMs from postnatal hearts. Here, we aimed to compare and optimize protocols for the isolation of CMs from embryonic to adult stages. We found that the trypsin digestion method was suitable for embryonic mouse CM isolation. The Gentle MACS method yielded high- quality CMs from neonatal hearts (postnatal day 1- day 3, P1-P3). (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 13, 2022 Category: Cytology Authors: Jie Feng, Yan Li, Yu Nie Source Type: research

Cardiovascular disease and the biology of aging
The incidence and prevalence of a wide range of cardiovascular diseases increases as a function of age. This well-established epidemiological relationship suggests that chronological aging might contribute or increase susceptibility to varied conditions such as atherosclerosis, vascular stiffening or heart failure. Here, we explore the mechanistic links that connect both rare and common cardiovascular conditions to the basic biology of aging. These links provide a rational basis to begin to develop a new set of therapeutics targeting the fundamental mechanisms underlying the aging process and suggest that in the near futur...
Source: Journal of Molecular and Cellular Cardiology - April 11, 2022 Category: Cytology Authors: Shria Moturi, Shohini K. Ghosh-Choudhary, Toren Finkel Tags: Review article Source Type: research

Aging and HFpEF: Are we running out of time?
The most significant determining factor of cardiovascular health is a person's age, with cardiovascular diseases being the leading cause of death in 40% of individuals over 65  years. Especially Heart Failure (HF) with preserved Ejection Fraction (HFpEF) is strongly age related, and in fact primarily affects elderly patients, with a predominance for women, and is further associated with multiple co-morbidities. HFpEF has become a leading cause of morbidity, hospitalizat ion and mortality in subjects at the age of>65  years [1]. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - April 11, 2022 Category: Cytology Authors: Coenraad Withaar, Sunhuo Li, Laura M.G. Meems, Herman H.W. Sillj é, Rudolf A. de Boer Tags: Letter to the editor Source Type: research