Methods of mouse cardiomyocyte isolation from postnatal heart

Primary cardiomyocytes (CMs) are invaluable materials used to study in vitro cardiac physiology and pathophysiology. However, there is still a lack of comprehensive, systematic and comparative studies on methods for isolation of primary CMs from postnatal hearts. Here, we aimed to compare and optimize protocols for the isolation of CMs from embryonic to adult stages. We found that the trypsin digestion method was suitable for embryonic mouse CM isolation. The Gentle MACS method yielded high- quality CMs from neonatal hearts (postnatal day 1- day 3, P1-P3).
Source: Journal of Molecular and Cellular Cardiology - Category: Cytology Authors: Source Type: research