Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity
J Leukoc Biol. 2024 Mar 30:qiae083. doi: 10.1093/jleuko/qiae083. Online ahead of print.ABSTRACTIdiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytomet...
Source: Journal of Leukocyte Biology - March 30, 2024 Category: Hematology Authors: Ra úl F Reyes-Huerta Vladimir Mandujano-L ópez Guadalupe Vel ásquez-Ortiz Beatriz Alcal á-Carmona Mar ía J Ostos-Prado Yatzil Reyna-Ju árez David E Meza-S ánchez Guillermo Ju árez-Vega Nancy R Mej ía-Domínguez Jiram Torres-Ruiz Diana G ómez-Mar Source Type: research
Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity
J Leukoc Biol. 2024 Mar 30:qiae083. doi: 10.1093/jleuko/qiae083. Online ahead of print.ABSTRACTIdiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytomet...
Source: Journal of Leukocyte Biology - March 30, 2024 Category: Hematology Authors: Ra úl F Reyes-Huerta Vladimir Mandujano-L ópez Guadalupe Vel ásquez-Ortiz Beatriz Alcal á-Carmona Mar ía J Ostos-Prado Yatzil Reyna-Ju árez David E Meza-S ánchez Guillermo Ju árez-Vega Nancy R Mej ía-Domínguez Jiram Torres-Ruiz Diana G ómez-Mar Source Type: research
Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity
J Leukoc Biol. 2024 Mar 30:qiae083. doi: 10.1093/jleuko/qiae083. Online ahead of print.ABSTRACTIdiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytomet...
Source: Journal of Leukocyte Biology - March 30, 2024 Category: Hematology Authors: Ra úl F Reyes-Huerta Vladimir Mandujano-L ópez Guadalupe Vel ásquez-Ortiz Beatriz Alcal á-Carmona Mar ía J Ostos-Prado Yatzil Reyna-Ju árez David E Meza-S ánchez Guillermo Ju árez-Vega Nancy R Mej ía-Domínguez Jiram Torres-Ruiz Diana G ómez-Mar Source Type: research
Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity
J Leukoc Biol. 2024 Mar 30:qiae083. doi: 10.1093/jleuko/qiae083. Online ahead of print.ABSTRACTIdiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytomet...
Source: Journal of Leukocyte Biology - March 30, 2024 Category: Hematology Authors: Ra úl F Reyes-Huerta Vladimir Mandujano-L ópez Guadalupe Vel ásquez-Ortiz Beatriz Alcal á-Carmona Mar ía J Ostos-Prado Yatzil Reyna-Ju árez David E Meza-S ánchez Guillermo Ju árez-Vega Nancy R Mej ía-Domínguez Jiram Torres-Ruiz Diana G ómez-Mar Source Type: research
Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity
J Leukoc Biol. 2024 Mar 30:qiae083. doi: 10.1093/jleuko/qiae083. Online ahead of print.ABSTRACTIdiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytomet...
Source: Journal of Leukocyte Biology - March 30, 2024 Category: Hematology Authors: Ra úl F Reyes-Huerta Vladimir Mandujano-L ópez Guadalupe Vel ásquez-Ortiz Beatriz Alcal á-Carmona Mar ía J Ostos-Prado Yatzil Reyna-Ju árez David E Meza-S ánchez Guillermo Ju árez-Vega Nancy R Mej ía-Domínguez Jiram Torres-Ruiz Diana G ómez-Mar Source Type: research
The neonatal liver hosts a spontaneously occurring neutrophil population, exhibiting distinct spatial and functional characteristics from adults
J Leukoc Biol. 2024 Mar 29:qiae082. doi: 10.1093/jleuko/qiae082. Online ahead of print.ABSTRACTThe elusive nature of the liver immune system in newborns remains an important challenge, casting a shadow over our understanding of how to effectively treat and prevent diseases in children. Therefore, deeper exploration into the intricacies of neonatal immunology might be crucial for improved pediatric healthcare. Using liver intravital microscopy, we unveiled a significant population of granulocytes in the hepatic parenchyma of fetuses and newborns. Utilizing high-dimensional immunophenotyping, we showed dynamic alterations pr...
Source: Journal of Leukocyte Biology - March 29, 2024 Category: Hematology Authors: Wanderson Ferreira da Silva J únior Maria Alice de Freitas Lopes Ma ísa Mota Antunes Karen Marques de Oliveira Costa Ariane Barros Diniz Brenda Naemi Lanza Nakagaki Camila Dutra Moreira de Miranda Hort ência Maciel de Castro Oliveira Alesandra Corte Re Source Type: research
The neonatal liver hosts a spontaneously occurring neutrophil population, exhibiting distinct spatial and functional characteristics from adults
J Leukoc Biol. 2024 Mar 29:qiae082. doi: 10.1093/jleuko/qiae082. Online ahead of print.ABSTRACTThe elusive nature of the liver immune system in newborns remains an important challenge, casting a shadow over our understanding of how to effectively treat and prevent diseases in children. Therefore, deeper exploration into the intricacies of neonatal immunology might be crucial for improved pediatric healthcare. Using liver intravital microscopy, we unveiled a significant population of granulocytes in the hepatic parenchyma of fetuses and newborns. Utilizing high-dimensional immunophenotyping, we showed dynamic alterations pr...
Source: Journal of Leukocyte Biology - March 29, 2024 Category: Hematology Authors: Wanderson Ferreira da Silva J únior Maria Alice de Freitas Lopes Ma ísa Mota Antunes Karen Marques de Oliveira Costa Ariane Barros Diniz Brenda Naemi Lanza Nakagaki Camila Dutra Moreira de Miranda Hort ência Maciel de Castro Oliveira Alesandra Corte Re Source Type: research
In vitro effect of diazoxon on cell signaling and second messengers in Nile tilapia (Oreochromis niloticus) leukocytes
In this study, the in vitro effects of DZO on molecules involved in cell signaling (cAMP, IP3, DAG, JAK1, and STAT3), which play a crucial role in the activation, differentiation, and survival of leukocytes, were evaluated. Data indicate that DZO leads to a decrease in cAMP concentration and an increase in basal IP3 levels. However, DZO does not affect basal levels of JAK1 and STAT3 phosphorylation. Instead, DZO inhibits leukocyte responsiveness to PMA and ionomycin, substances that, under normal conditions, enhance JAK/STAT signaling. These findings demonstrate that DZO significantly affects key molecular parameters relat...
Source: Journal of Leukocyte Biology - March 28, 2024 Category: Hematology Authors: Milton Rafael Camacho-P érez Karina Janice Guadalupe D íaz-Resendiz Roc ío Ortiz-Butrón Carlos Eduardo Covantes-Rosales Alma Betsaida Benitez-Trinidad Daniel Alberto Gir ón-Pérez Gladys Alejandra Toledo-Ibarra Lenin Pav ón Manuel Iv án Girón-Pér Source Type: research
Eosinophil expression of Triggering receptor expressed on myeloid cells-1 (TREM-1) restricts type 2 lung inflammation
J Leukoc Biol. 2024 Mar 28:qiae061. doi: 10.1093/jleuko/qiae061. Online ahead of print.ABSTRACTAsthma affects 25 million Americans and recent advances in treatment are effective for only a portion of severe asthma patients. Triggering Receptor Expressed on Myeloid cells 1 (TREM-1), an innate receptor that canonically amplifies inflammatory signaling in neutrophils and monocytes, plays a central role in regulating lung inflammation. It is unknown how TREM-1 contributes to allergic asthma pathology. Utilizing a murine model of asthma, flow cytometry revealed TREM-1+ eosinophils in the lung tissue and airway during allergic a...
Source: Journal of Leukocyte Biology - March 28, 2024 Category: Hematology Authors: Jayden L Bowen Kathy Keck Sankar Baruah Kathy H Nguyen Andrew L Thurman Alejandro A Pezzulo Julia Klesney-Tait Source Type: research
In vitro effect of diazoxon on cell signaling and second messengers in Nile tilapia (Oreochromis niloticus) leukocytes
In this study, the in vitro effects of DZO on molecules involved in cell signaling (cAMP, IP3, DAG, JAK1, and STAT3), which play a crucial role in the activation, differentiation, and survival of leukocytes, were evaluated. Data indicate that DZO leads to a decrease in cAMP concentration and an increase in basal IP3 levels. However, DZO does not affect basal levels of JAK1 and STAT3 phosphorylation. Instead, DZO inhibits leukocyte responsiveness to PMA and ionomycin, substances that, under normal conditions, enhance JAK/STAT signaling. These findings demonstrate that DZO significantly affects key molecular parameters relat...
Source: Journal of Leukocyte Biology - March 28, 2024 Category: Hematology Authors: Milton Rafael Camacho-P érez Karina Janice Guadalupe D íaz-Resendiz Roc ío Ortiz-Butrón Carlos Eduardo Covantes-Rosales Alma Betsaida Benitez-Trinidad Daniel Alberto Gir ón-Pérez Gladys Alejandra Toledo-Ibarra Lenin Pav ón Manuel Iv án Girón-Pér Source Type: research
Eosinophil expression of Triggering receptor expressed on myeloid cells-1 (TREM-1) restricts type 2 lung inflammation
J Leukoc Biol. 2024 Mar 28:qiae061. doi: 10.1093/jleuko/qiae061. Online ahead of print.ABSTRACTAsthma affects 25 million Americans and recent advances in treatment are effective for only a portion of severe asthma patients. Triggering Receptor Expressed on Myeloid cells 1 (TREM-1), an innate receptor that canonically amplifies inflammatory signaling in neutrophils and monocytes, plays a central role in regulating lung inflammation. It is unknown how TREM-1 contributes to allergic asthma pathology. Utilizing a murine model of asthma, flow cytometry revealed TREM-1+ eosinophils in the lung tissue and airway during allergic a...
Source: Journal of Leukocyte Biology - March 28, 2024 Category: Hematology Authors: Jayden L Bowen Kathy Keck Sankar Baruah Kathy H Nguyen Andrew L Thurman Alejandro A Pezzulo Julia Klesney-Tait Source Type: research
Evidence that keratinocyte microvesicle particles carrying Platelet-activating factor mediate the widespread multi-organ damage associated with intoxicated thermal burn injury
J Leukoc Biol. 2024 Mar 26:qiae078. doi: 10.1093/jleuko/qiae078. Online ahead of print.ABSTRACTThermal burn injuries can result in significant morbidity and mortality. The combination of ethanol intoxication with thermal burn injury results in increased morbidity through an exaggerated inflammatory response involving many organs. Recent studies have linked involvement of the lipid mediator Platelet-activating factor (PAF) in the pathology associated with intoxicated thermal burn injury (ITBI). The present studies tested the roles of PAF and the elevated levels of subcellular microvesicle particles (MVP) generated in respon...
Source: Journal of Leukocyte Biology - March 26, 2024 Category: Hematology Authors: Rushabh P Lohade Chad Brewer Christine M Rapp Karen M Henkels Wenfeng Zhang Anita Thyagarajan Shikshita Singh Pranali Manjrekar Taskin Sabit Ravi P Sahu Jeffrey B Travers Source Type: research
Evidence that keratinocyte microvesicle particles carrying Platelet-activating factor mediate the widespread multi-organ damage associated with intoxicated thermal burn injury
J Leukoc Biol. 2024 Mar 26:qiae078. doi: 10.1093/jleuko/qiae078. Online ahead of print.ABSTRACTThermal burn injuries can result in significant morbidity and mortality. The combination of ethanol intoxication with thermal burn injury results in increased morbidity through an exaggerated inflammatory response involving many organs. Recent studies have linked involvement of the lipid mediator Platelet-activating factor (PAF) in the pathology associated with intoxicated thermal burn injury (ITBI). The present studies tested the roles of PAF and the elevated levels of subcellular microvesicle particles (MVP) generated in respon...
Source: Journal of Leukocyte Biology - March 26, 2024 Category: Hematology Authors: Rushabh P Lohade Chad Brewer Christine M Rapp Karen M Henkels Wenfeng Zhang Anita Thyagarajan Shikshita Singh Pranali Manjrekar Taskin Sabit Ravi P Sahu Jeffrey B Travers Source Type: research
Buena VISTA: A Promising Outlook on Targeting Immune Checkpoint Regulators to Combat Sepsis
J Leukoc Biol. 2024 Mar 25:qiae080. doi: 10.1093/jleuko/qiae080. Online ahead of print.NO ABSTRACTPMID:38526166 | DOI:10.1093/jleuko/qiae080 (Source: Journal of Leukocyte Biology)
Source: Journal of Leukocyte Biology - March 25, 2024 Category: Hematology Authors: Madeline D Mott Briana Trusiano Irving Coy Allen Source Type: research
Relative contributions of osteal macrophages and osteoclasts to postnatal bone development in CSF1R-deficient rats and phenotype rescue following wild-type bone marrow cell transfer
J Leukoc Biol. 2024 Mar 25:qiae077. doi: 10.1093/jleuko/qiae077. Online ahead of print.ABSTRACTMacrophage and osteoclast proliferation, differentiation and survival are regulated by colony-stimulating factor-1 receptor (CSF1R) signaling. Osteopetrosis associated with Csf1 and Csf1r mutations has been attributed to the loss of osteoclasts and deficiency in bone resorption. Here we demonstrate that homozygous Csf1r mutation in rat leads to delayed postnatal skeletal ossification associated with substantial loss of osteal macrophages (osteomacs) in addition to osteoclasts. Osteosclerosis and site-specific skeletal abnormaliti...
Source: Journal of Leukocyte Biology - March 25, 2024 Category: Hematology Authors: Lena Batoon Sahar Keshvari Katharine M Irvine Eileen Ho Melanie Caruso Omkar L Patkar Anuj Sehgal Susan M Millard David A Hume Allison R Pettit Source Type: research
