Human CD34+/dim neutrophil-committed progenitors do not differentiate into neutrophil-like CXCR1 + CD14 + CD16- monocytes in vitro
In this study, by performing a more analytical reevaluation at the phenotypic and molecular levels of the cells generated by NCP2 s and NCP4 s (selected as representatives of NCPs), we categorically exclude that NCPs generate neutrophil-like CXCR1 + CD14 + CD16-monocytes. Rather, we provide substantial evidence indicating that the cells generated by NePs/alias "early neutrophil-committed progenitors" are neutrophilic cells at different stage of maturation, displaying moderate levels of CD14, instead of neutrophil-like CXCR1 + CD14 + CD16-monocytes as pointed by Ikeda and coworkers. Hence, the conclusion that NePs/alias "ea...
Source: Journal of Leukocyte Biology - December 19, 2023 Category: Hematology Authors: Ilaria Signoretto Federica Calzetti Sara Gasperini Francisco Bianchetto-Aguilera Elisa Gardiman Giulia Finotti Cristina Tecchio Nicola Tamassia Marco A Cassatella Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
Enhancing the efficacy of vaccinia-based oncolytic virotherapy by inhibiting CXCR2-mediated MDSC trafficking
J Leukoc Biol. 2023 Dec 8:qiad150. doi: 10.1093/jleuko/qiad150. Online ahead of print.ABSTRACTOncolytic virotherapy is an innovative approach for cancer treatment. However, recruitment of myeloid derived suppressor cells (MDSC) into the tumor microenvironment (TME) after oncolysis-mediated local inflammation leads to tumor resistance to the therapy. Using the murine malignant mesothelioma model, we demonstrated that the in-situ vaccinia virotherapy recruited primarily polymorphonuclear MDSC (PMN-MDSC) into the TME where they exhibited strong suppression of cytotoxic T lymphocytes (CTL) in a reactive oxygen species (ROS)-de...
Source: Journal of Leukocyte Biology - December 9, 2023 Category: Hematology Authors: Zhiwu Tan Mei Sum Chiu Ming Yue Hau Yee Kwok Man Ho Tse Yang Wen Bohao Chen Dawei Yang Dongyan Zhou You-Qiang Song Kwan Man Zhiwei Chen Source Type: research
Effect of Ethanol-exposure on Innate Immune Response in Sepsis
J Leukoc Biol. 2023 Dec 8:qiad156. doi: 10.1093/jleuko/qiad156. Online ahead of print.ABSTRACTAlcohol use disorder, reported by one in eight critically ill patients, is a risk factor for death in sepsis patients. Sepsis, the leading cause of death kills over 270,000 patients in the United States alone and remains without targeted therapy. Immune response in sepsis transitions from an early hyper-inflammation to persistent inflammation and immunosuppression and multiple organ dysfunction during late sepsis. Innate immunity is the first line of defense against pathogen invasion. Ethanol exposure is known to impair innate and...
Source: Journal of Leukocyte Biology - December 9, 2023 Category: Hematology Authors: Sanjoy Roychowdhury Bishnu Pant Emily Cross Rachel Scheraga Vidula Vachharajani Source Type: research
Enhancing the efficacy of vaccinia-based oncolytic virotherapy by inhibiting CXCR2-mediated MDSC trafficking
J Leukoc Biol. 2023 Dec 8:qiad150. doi: 10.1093/jleuko/qiad150. Online ahead of print.ABSTRACTOncolytic virotherapy is an innovative approach for cancer treatment. However, recruitment of myeloid derived suppressor cells (MDSC) into the tumor microenvironment (TME) after oncolysis-mediated local inflammation leads to tumor resistance to the therapy. Using the murine malignant mesothelioma model, we demonstrated that the in-situ vaccinia virotherapy recruited primarily polymorphonuclear MDSC (PMN-MDSC) into the TME where they exhibited strong suppression of cytotoxic T lymphocytes (CTL) in a reactive oxygen species (ROS)-de...
Source: Journal of Leukocyte Biology - December 9, 2023 Category: Hematology Authors: Zhiwu Tan Mei Sum Chiu Ming Yue Hau Yee Kwok Man Ho Tse Yang Wen Bohao Chen Dawei Yang Dongyan Zhou You-Qiang Song Kwan Man Zhiwei Chen Source Type: research
Effect of Ethanol-exposure on Innate Immune Response in Sepsis
J Leukoc Biol. 2023 Dec 8:qiad156. doi: 10.1093/jleuko/qiad156. Online ahead of print.ABSTRACTAlcohol use disorder, reported by one in eight critically ill patients, is a risk factor for death in sepsis patients. Sepsis, the leading cause of death kills over 270,000 patients in the United States alone and remains without targeted therapy. Immune response in sepsis transitions from an early hyper-inflammation to persistent inflammation and immunosuppression and multiple organ dysfunction during late sepsis. Innate immunity is the first line of defense against pathogen invasion. Ethanol exposure is known to impair innate and...
Source: Journal of Leukocyte Biology - December 9, 2023 Category: Hematology Authors: Sanjoy Roychowdhury Bishnu Pant Emily Cross Rachel Scheraga Vidula Vachharajani Source Type: research
AIRE is expressed in breast cancer TANs and TAMs to regulate the extrinsic apoptotic pathway and inflammation
In this study we show that AIRE is expressed in human pro-tumor neutrophils. In breast cancer, AIRE was primarily located to tumor associated neutrophils (TANs), and to a lesser extent to tumor associated macrophages (TAMs) and tumor cells. Expression of AIRE in TAN/TAMs, but not in cancer cells, was associated with an adverse prognosis. We show that the functional role for AIRE in neutrophils and macrophages is to regulate expression of immune mediators and the extrinsic apoptotic pathway involving the Fas/TNFR death receptors and Cathepsin G. We here propose that the role for AIRE in TAN/TAMs in breast tumors is to regul...
Source: Journal of Leukocyte Biology - December 7, 2023 Category: Hematology Authors: Eva K ällberg Meliha Mehmeti-Ajradini Frida Bj örk Gunnarsdottir Marcus G öransson Caroline Bergenfelz Roni Allaoui Fredriksson Catharina Hagerling Martin E Johansson Charlotte Welinder Karin Jirstr öm Karin Leandersson Source Type: research
AIRE is expressed in breast cancer TANs and TAMs to regulate the extrinsic apoptotic pathway and inflammation
In this study we show that AIRE is expressed in human pro-tumor neutrophils. In breast cancer, AIRE was primarily located to tumor associated neutrophils (TANs), and to a lesser extent to tumor associated macrophages (TAMs) and tumor cells. Expression of AIRE in TAN/TAMs, but not in cancer cells, was associated with an adverse prognosis. We show that the functional role for AIRE in neutrophils and macrophages is to regulate expression of immune mediators and the extrinsic apoptotic pathway involving the Fas/TNFR death receptors and Cathepsin G. We here propose that the role for AIRE in TAN/TAMs in breast tumors is to regul...
Source: Journal of Leukocyte Biology - December 7, 2023 Category: Hematology Authors: Eva K ällberg Meliha Mehmeti-Ajradini Frida Bj örk Gunnarsdottir Marcus G öransson Caroline Bergenfelz Roni Allaoui Fredriksson Catharina Hagerling Martin E Johansson Charlotte Welinder Karin Jirstr öm Karin Leandersson Source Type: research
