Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
J Leukoc Biol. 2023 Dec 21:qiad159. doi: 10.1093/jleuko/qiad159. Online ahead of print.ABSTRACTThe chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps - rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, that exists as ...
Source: Journal of Leukocyte Biology - December 21, 2023 Category: Hematology Authors: Iliana I Le ón-Vega Eduardo Vadillo Hilda Vargas-Robles Krishna Rajarathnam Michael Schnoor Source Type: research
Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
J Leukoc Biol. 2023 Dec 21:qiad159. doi: 10.1093/jleuko/qiad159. Online ahead of print.ABSTRACTThe chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps - rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, that exists as ...
Source: Journal of Leukocyte Biology - December 21, 2023 Category: Hematology Authors: Iliana I Le ón-Vega Eduardo Vadillo Hilda Vargas-Robles Krishna Rajarathnam Michael Schnoor Source Type: research
Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
J Leukoc Biol. 2023 Dec 21:qiad159. doi: 10.1093/jleuko/qiad159. Online ahead of print.ABSTRACTThe chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps - rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, that exists as ...
Source: Journal of Leukocyte Biology - December 21, 2023 Category: Hematology Authors: Iliana I Le ón-Vega Eduardo Vadillo Hilda Vargas-Robles Krishna Rajarathnam Michael Schnoor Source Type: research
Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
J Leukoc Biol. 2023 Dec 21:qiad159. doi: 10.1093/jleuko/qiad159. Online ahead of print.ABSTRACTThe chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps - rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, that exists as ...
Source: Journal of Leukocyte Biology - December 21, 2023 Category: Hematology Authors: Iliana I Le ón-Vega Eduardo Vadillo Hilda Vargas-Robles Krishna Rajarathnam Michael Schnoor Source Type: research
Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
J Leukoc Biol. 2023 Dec 21:qiad159. doi: 10.1093/jleuko/qiad159. Online ahead of print.ABSTRACTThe chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps - rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, that exists as ...
Source: Journal of Leukocyte Biology - December 21, 2023 Category: Hematology Authors: Iliana I Le ón-Vega Eduardo Vadillo Hilda Vargas-Robles Krishna Rajarathnam Michael Schnoor Source Type: research
Human CD34+/dim neutrophil-committed progenitors do not differentiate into neutrophil-like CXCR1 + CD14 + CD16- monocytes in vitro
In this study, by performing a more analytical reevaluation at the phenotypic and molecular levels of the cells generated by NCP2 s and NCP4 s (selected as representatives of NCPs), we categorically exclude that NCPs generate neutrophil-like CXCR1 + CD14 + CD16-monocytes. Rather, we provide substantial evidence indicating that the cells generated by NePs/alias "early neutrophil-committed progenitors" are neutrophilic cells at different stage of maturation, displaying moderate levels of CD14, instead of neutrophil-like CXCR1 + CD14 + CD16-monocytes as pointed by Ikeda and coworkers. Hence, the conclusion that NePs/alias "ea...
Source: Journal of Leukocyte Biology - December 19, 2023 Category: Hematology Authors: Ilaria Signoretto Federica Calzetti Sara Gasperini Francisco Bianchetto-Aguilera Elisa Gardiman Giulia Finotti Cristina Tecchio Nicola Tamassia Marco A Cassatella Source Type: research
Human CD34+/dim neutrophil-committed progenitors do not differentiate into neutrophil-like CXCR1 + CD14 + CD16- monocytes in vitro
In this study, by performing a more analytical reevaluation at the phenotypic and molecular levels of the cells generated by NCP2 s and NCP4 s (selected as representatives of NCPs), we categorically exclude that NCPs generate neutrophil-like CXCR1 + CD14 + CD16-monocytes. Rather, we provide substantial evidence indicating that the cells generated by NePs/alias "early neutrophil-committed progenitors" are neutrophilic cells at different stage of maturation, displaying moderate levels of CD14, instead of neutrophil-like CXCR1 + CD14 + CD16-monocytes as pointed by Ikeda and coworkers. Hence, the conclusion that NePs/alias "ea...
Source: Journal of Leukocyte Biology - December 19, 2023 Category: Hematology Authors: Ilaria Signoretto Federica Calzetti Sara Gasperini Francisco Bianchetto-Aguilera Elisa Gardiman Giulia Finotti Cristina Tecchio Nicola Tamassia Marco A Cassatella Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Elucidating granulocytic myeloid-derived suppressor cell heterogeneity during Staphylococcus aureus biofilm infection
In this study, we identified a previously unknown population of Ly6G + Ly6C + F4/80 + MHCII+ MDSCs during PJI that displayed immunosuppressive properties ex vivo. We leveraged F4/80 and MHCII expression by these cells for further characterization using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), which revealed a distinct transcriptomic signature of this population. F4/80 + MHCII+ MDSCs displayed gene signatures resembling G-MDSCs, neutrophils, and monocytes, but had significantly increased expression of pathways involved in cytokine response/production, inflammatory cell death, and mononuclea...
Source: Journal of Leukocyte Biology - December 14, 2023 Category: Hematology Authors: Blake P Bertrand Cortney E Heim Scott A Koepsell Tammy Kielian Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
Unraveling the secrets of γδ T cells with single-cell biology
J Leukoc Biol. 2023 Oct 24:qiad131. doi: 10.1093/jleuko/qiad131. Online ahead of print.ABSTRACTRecent technological advancements have enabled us to study the molecular features of cellular states at the single-cell level, providing unprecedented resolution for comprehending the identity and function of a cell. By applying these techniques across multiple time frames, tissues, and diseases, we can delve deeper into the mechanisms governing the development and functions of cell lineages. In this review, I focus on γδ T cells, which are a unique and functionally nonredundant T cell lineage categorized under the umbrella of ...
Source: Journal of Leukocyte Biology - December 11, 2023 Category: Hematology Authors: None Sagar Source Type: research
