Disturbed Transcription of TLRs' Negative Regulators and Cytokines Secretion among TLR4- and 9-Activated PBMCs of Agammaglobulinemic Patients.
Authors: Sanaei R, Rezaei N, Aghamohammadi A, Delbandi AA, Tavasolian P, Tajik N Abstract Toll-like receptors (TLRs) are inevitable elements for immunity development and antibody production. TLRs are in close interaction with Bruton's tyrosine kinase which has been found mutated and malfunctioned in the prototype antibody deficiency disease named X-linked agammaglobulinemia (XLA). TLRs' ability was evaluated to induce transcription of TLR-negative regulators, including suppressor of cytokine signaling 1 (SOCS1), interleukin-1 receptor-associated kinase 3 (IRAK-M), tumor necrosis factor alpha-induced protein 3 (TNFA...
Source: Immunological Investigations - June 13, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Dendritic Cells Generated in the Presence of Platelet Lysate Have a Reduced Type 1 Polarization Capacity.
Authors: Tešić N, Pekle Simonič I, Roškar K, Rožman P, Švajger U Abstract Previously, we have shown platelet lysate (PL) can be used as a non-xenogeneic serum supplement for generation of monocyte-derived dendritic cells (DCs). Since DC-based activation protocols are extremely sensitive to microenvironmental changes such as replacement of culture medium, we wanted to examine the behavior of DCs cultured in the presence of PL under various type-1 activation conditions and assess their type 1 polarization capacity. We compared the quality of DCs cultured in 10% PL-supplemented RPMI medium (plD...
Source: Immunological Investigations - June 8, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

HLA Class II Allele and Haplotype Diversity in Libyans and Their Genetic Relationships with Other Populations.
Conclusion: Libyans appear to be closely related to North Africans, Saudis, and Iberians, but distinct from Levantine Arabs, East Mediterraneans, and Sub-Saharan Africans. This indicates limited genetic contribution of Levantine Arabs and Sub-Saharans on the makeup of Libyan gene pool. Our study confirmed genetic heterogeneity among Arab populations, with three identified groups. The first comprises North Africans, Saudis, and Kuwaitis who were related to Iberians and West Mediterraneans, while the second consists of Levantine Arabs who were close to East Mediterraneans, and the third contained Sudanese and Comorians, with...
Source: Immunological Investigations - June 6, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Devil Facial Tumours: Towards a Vaccine.
Authors: Owen RS, Siddle HV Abstract The Tasmanian devil is the only mammalian species to harbour two independent lineages of contagious cancer. Devil facial tumour 1 (DFT1) emerged in the 1990s and has caused significant population declines. Devil facial tumour 2 (DFT2) was identified in 2014, and evidence indicates that this new tumour has emerged independently of DFT1. While DFT1 is widespread across Tasmania, DFT2 is currently found only on the Channel Peninsula in south east Tasmania. Allograft transmission of cancer cells should be prevented by major histocompatibility complex (MHC) molecules. DFT1 avoids imm...
Source: Immunological Investigations - June 6, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Impact of IL12B Polymorphisms on Genetic Susceptibility and IL-12p40 and IL-23 Serum Levels in Rheumatoid Arthritis.
In conclusion, our study demonstrates a functional effect of IL12B polymorphisms on IL-12p40 and IL-23 cytokine levels in RA patients and suggests a leading role for IL12B rs17860508 in the genetic predisposition to RA, while IL12B rs3212227 significantly modify the RA risk in Bulgarian population. PMID: 31161840 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - June 6, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Changing microRNA Expression during Three-Month Wasp Venom Immunotherapy.
Authors: Specjalski K, Maciejewska A, Pawłowski R, Zieliński M, Trzonkowski P, Pikuła M, Jassem E Abstract MicroRNAs are small non-coding molecules playing a significant regulatory role in several allergic diseases. However their role in tolerance induction remains unclear. The aim of this study was to determine the expression of selected microRNAs during the first three months of wasp venom immunotherapy (VIT). 5 adult patients with a history of severe systemic reactions after stinging by wasps and confirmed sensitization were included. Venous blood samples were collected before VIT, 24 hours after completing i...
Source: Immunological Investigations - May 29, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Sodium Butyrate as a Histone Deacetylase Inhibitor Affects Toll-Like Receptor 4 Expression in Colorectal Cancer Cell Lines.
This study focuses on the dose-time-effect of SB in the pathogenesis of colorectal cancer. SB alters the expression level of TLR4 in colorectal cancer cells. This effect may depend on the cell type, treatment duration and SB concentration. The alterations in TLR4 expression may be due to the direct effect of SB on TLR4 and/or the expression changes of in other genes which may indirectly affect the TLR4 expression. Abbreviations: TLR4: Toll-like receptor 4; HDACi: histone deacetylase inhibitor; SB: sodium Butyrate; CRC: colorectal cancer; SCFA: short-chain fatty acid; hrs: hours. PMID: 31117848 [PubMed - as supplied by ...
Source: Immunological Investigations - May 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of HLA-G Polymorphisms in the 3'UTR Region and Soluble HLA-G with Kidney Graft Outcome.
CONCLUSION: The study demonstrated genetic association between HLA-G 3'UTR variants and sHLA-G level in kidney recipients leading to graft acceptance. We suggest to monitor the pretransplantation sHLA-G level as additional marker to predict kidney graft outcome. ABBREVIATIONS: AMR: Antibody-mediated rejection; APC: antigen-presenting cell; CD: cluster of designation; del: deletion; HLA: human leukocyte antigen; ILT: immunoglobulin-like transcript; ins: insertion; KIR: killer-cell immunoglobulin-like receptor; NK: natural killer; sHLA-G: soluble HLA-G; SNP: single n...
Source: Immunological Investigations - May 17, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of CTLA-4 and CD28 Gene Polymorphisms with Type 1 Diabetes in South Indian Population.
Authors: Gunavathy N, Asirvatham A, Chitra A, Jayalakshmi M Abstract Each functional gene illustrates the complexity of genetic predisposition to disease; however, it is difficult to bring out these traits with reference to autoimmune diseases like type 1 diabetes (T1D). To find out the genetic contribution of CTLA-4 + 49A/G, CTLA-4 -318C/T and CD28 + 17T/C polymorphisms toward T1D, the present study was performed with 124 T1D patients, 54 siblings and 125 parents including 39 trios in South Indian population. The association and linkage of CTLA-4 + 49A/G, CTLA-4 -318C/T and CD28 ...
Source: Immunological Investigations - May 17, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Poly Lactic-co-Glycolic Acid Nanoparticles Containing Human Gastric Tumor Lysates as Antigen Delivery Vehicles for Dendritic Cell-Based Antitumor Immunotherapy.
In this study, poly lactic-co-glycolic acid nanoparticles (NPs) were used as DC antigen delivery vehicles in a preclinical model of immunotherapy of gastric cancer. The DCs were generated from peripheral blood monocytes by conventional in vitro differentiation and loaded with either soluble tumor lysate or lysate encapsulated in NPs using a double emulsion/solvent evaporation technique. Morphology of NPs was determined by scanning electron microscopy. Tumor lysate, either in the soluble form or encapsulated in NPs, was loaded into DC and stimulatory capacity was compared using patient-derived autologous CD3+ T cells as res...
Source: Immunological Investigations - May 17, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Aberrant Phenotype and Function of Dendritic Cells in Adult B Lineage Acute Lymphoblastic Leukemia.
Authors: Zhou Z, Lin W, Li X, Huang Y, Ren J, Gao Y, Li J Abstract Dendritic cells (DCs) play a major role in regulating immune responses, but the aberrant phenotype and function of defective DCs in adult acute lymphoblastic leukemia (ALL) remain unclear. Here, B lineage ALL (B-ALL) patients were divided into groups according to different standards. By course of disease: newly diagnosed (ND), complete remission (CR), consolidation (CONS). By stratification: high risk (HR), standard risk (SR). By minimal residual disease (MRD): MRD positive(MRD+), MRD negative (MRD-). The proportion of plasmacytoid DC(pDC) and myelo...
Source: Immunological Investigations - May 9, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Expression of ILT4 in Myeloid Dendritic Cells in Patients with Hepatocellular Carcinoma.
In this study, the frequency of mDCs subsets in the peripheral blood of 121 patients with HCC and 103 normal controls, and in the tumor and tumor free liver tissues (TFL) of 43 HCC patients was analyzed by flow cytometry. Then, the expressions of ILT4 in mDCs subsets in the microenvironment of liver cancer were also analyzed. Results showed that the percentage of CD1c+ subset was dramatically decreased in peripheral blood mononuclear cells (PBMCs) of HCC patients compared with normal controls, and also significantly decreased in tumor tissue compared with the TFL. The decreased of CD1c+ subset in blood could be a diagnosti...
Source: Immunological Investigations - May 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association between T-Cell Immunoglobulin and Mucin Domain 3 (TIM-3) Genetic Polymorphisms and Susceptibility to Autoimmune Diseases.
CONCLUSION: These findings reveal that TIM-3 + 4259A>C might be a potential susceptible predictor of ADs and especially RA. Further functional and clinical investigation between these diseases and TIM-3 polymorphisms is warranted. PMID: 31044630 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - May 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association between Genetic Polymorphisms of Inflammatory Response Genes and Acute Pancreatitis.
In conclusion, these results point to a plausible role for genetic polymorphisms in IL23R and RIPK2 in the development and severity of AP. PMID: 31044631 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - May 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Dynamic Aspects of the Immunoglobulin Structure.
Authors: Nezlin R Abstract Immunoglobulin (Ig) molecules are composed of Fab and Fc portions tethered by a hinge region that enables them to rotate and flex, relative to each other. Variable (V) and constant (C) domains of the Fab are connected by a flexible elbow region that is responsible for the movements of the V and C heterodimers. Significant movements of Fc domains have also been documented. The Ig portion's rotational freedom greatly enhances its ability to react with antigens and cell receptors, often simultaneously. The antigen-combining site also displays a dynamic structure. The ability of its various p...
Source: Immunological Investigations - May 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Interleukin-1 β Gene (IL1B) Polymorphism and Risk of Developing Diabetic Nephropathy.
CONCLUSION: In a studied population the -511 C/T polymorphism in the IL1B gene is associated with diabetic nephropathy in dialyzed T2DM patients. Further studies are required to confirm the clinical significance of this finding. PMID: 31044637 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - May 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Association between Genetic Variation in Wnt Transcription Factor TCF7L2 (TCF4) and Alopecia Areata.
CONCLUSIONS: This study indicates that the TCF7L2 gene variant is associated with AA. Its contribution to disease pathogenesis could either be through a hair cycling defect or dendritic cell dysregulation. PMID: 31012334 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

MicroRNA-31 Negatively Regulates Interleukin-34 Expression In Vitro.
Authors: Li M, Dong Y, Chen Z, Meng L, Liu X, Zhang X, Wang H, Mao W, Zhang J, Jiang Z, Huang T, Hu J, Luo P, Korner H, Ying S, Li J Abstract Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of I...
Source: Immunological Investigations - April 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Analysis of Inflammatory Gene Expression Profile of Peripheral Blood Leukocytes in Type 2 Diabetes.
This study was designed to perform quantitative PCR-based expression profiling of genes involved in inflammation (i.e. CASP1, CASP5, CCL5, CXC11, CCR5, NF-Κb, IL-4, PPARG and PGC1α) in peripheral blood leukocytes of T2DM patients. The T2DM patients are often prescribed with metformin and insulin while metformin has also been reported to possess anti-inflammatory activity. To address the question whether metformin exerts any effect on inflammatory mediators in bloodstream, human subjects in this study were divided into four groups on the basis of medication they were taking during last 6 month. These groups incl...
Source: Immunological Investigations - April 10, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

A Meta-Analysis of Cardiovascular Events in Systemic Lupus Erythematosus.
CONCLUSION: Based on the findings from previous reports, our meta-analysis showed that patients with SLE have been at higher risk of CV events. PMID: 30961407 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 10, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Effects of Silymarin and Cyclosporine A on the Proliferation and Cytokine Production of Regulatory T Cells.
Authors: Keyhanmehr N, Motedayyen H, Eskandari N Abstract BACKGROUND: Immunosuppressive agents are necessary to enhance allograft tolerance after transplantation and the treatment of autoimmune disorders. Regulatory T cells (Tregs) play a pivotal role in improving allograft tolerance and determining the fate of transplanted organs. Therefore, the aim of this study was to investigate the immunomodulatory effects of cyclosporine A (CsA) and silymarin on the proliferation and cytokine production of Tregs. METHODS: Peripheral blood mononuclear cells (PBMCs) were obtained from healthy voluntaries and Tregs were isol...
Source: Immunological Investigations - April 7, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Impact of HLA-G 3'UTR Polymorphisms in Breast Cancer in a Tunisian Population.
Authors: Ouni N, Chaaben AB, Kablouti G, Ayari F, Douik H, Abaza H, Gara S, Elgaaied-Benammar A, Guemira F, Tamouza R Abstract Human leukocyte antigens G and E (HLA-G and HLA-E) are nonclassical major histocompatibility complex (MHC) class I molecules. These molecules play an important role in immune surveillance by inhibiting natural killer and cytotoxic T cells responsible for immune escape. The expression of HLA-G and HLA-E has been associated with several diseases including tumor. The main objective of the study is to evaluate the impact of three HLA-G 3'UTR potential polymorphisms: +3187 A> G (rs938014...
Source: Immunological Investigations - April 6, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Effect of IL-26 on TNF- α-Induced CXCL8 Responses by Colonic Epithelial Cell Lines.
The Effect of IL-26 on TNF-α-Induced CXCL8 Responses by Colonic Epithelial Cell Lines. Immunol Invest. 2019 Mar 27;:1-13 Authors: Weishaar IM, Young RS, Wiles BM, McGee DW Abstract Th17 cells of the intestine and colon can produce several important cytokines during mucosal inflammation. However, few studies have focused on the role of IL-26 in intestinal inflammations. Colonic epithelial cells express receptors for IL-26, and this cytokine has been shown to induce the HT-29 colonic epithelial cell line to produce the chemokine CXCL8. However, epithelial cells would function in a cytokine network ...
Source: Immunological Investigations - March 30, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Immuno-Enhancement Effects of Tubiechong (Eupolyphaga sinensis) Lyophilized Powder in Cyclophosphamide-Induced Immunosuppressed Mice.
Authors: Liu H, Yan Y, Zhang F, Wu Q Abstract Tubiechong (Eupolyphaga sinensis) is an important material used in traditional Chinese medicine (TCM). However, the immunoregulation effects of E. sinensis Lyophilized Powder (ESL) are unclear. The in vivo study thus designed to elucidate the immuno-enhancement effects of ESL in immunosuppressed mice induced by cyclophosphamide (CTX). Mice were treated with three doses of ESL (0.5, 1.0 and 2.0 g/kg). Compared with model group, ESL notably increased the immune organ index, mononuclear macrophages function and the level of nature killer cell (NK) (p
Source: Immunological Investigations - March 30, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Autophagy-Related 5 Gene rs510432 Polymorphism Is Associated with Hepatocellular Carcinoma in Patients with Chronic Hepatitis B Virus Infection.
CONCLUSION: These results indicate that rs510432 genotypes AA+GA are associated with disease progression and HCC risk in chronic HBV infection, providing novel evidence for a role of ATG5 in the pathogenesis of HBV-related HCC. ABBREVIATIONS: HBV: hepatitis B virus; HCC hepatocellular carcinoma; TNFSF10: tumor necrosis factor superfamily member 10; ATG5: autophagy-related protein 5; DNA: deoxyribonucleic acid; LDR-PCR: ligase detection reactions-polymerase chain reaction; PCR: polymerase chain reaction; SLE: systemic lupus erythematosus; BD: Behçet's disease; IL-10: interlukin-10; LPS: lipopolysaccharide; PBMC: ...
Source: Immunological Investigations - March 27, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

ECTV Abolishes the Ability of GM-BM Cells to Stimulate Allogeneic CD4 T Cells in a Mouse Strain-Independent Manner.
Authors: Szulc-Dąbrowska L, Wojtyniak P, Struzik J, Toka FN, Winnicka A, Gieryńska M Abstract Ectromelia virus (ECTV) is the etiological agent of mousepox, an acute and systemic disease with high mortality rates in susceptible strains of mice. Resistance and susceptibility to mousepox are triggered by the dichotomous T-helper (Th) immune response generated in infected animals, with strong protective Th1 or nonprotective Th2 profile, respectively. Th1/Th2 balance is influenced by dendritic cells (DCs), which were shown to differ in their ability to polarize naïve CD4+ T cells in different mouse strains. There...
Source: Immunological Investigations - March 21, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The First Purine Nucleoside Phosphorylase Deficiency Patient Resembling IgA Deficiency and a Review of the Literature.
In this study, whole-exome sequencing was used to detect mutation in the case suspected of having primary immunodeficiency. We found a homozygous mutation in PNP gene in a girl who is the third case from the national Iranian registry. She had combined immunodeficiency, autoimmune hemolytic anemia and a history of recurrent infections. She developed no neurological dysfunction. She died at the age of 11 after a severe chicken pox infection. PNP deficiency should be considered in late-onset children with recurrent infections, autoimmune disorders without typical neurologic impairment. PMID: 30885031 [PubMed - as supplied...
Source: Immunological Investigations - March 21, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Exogenous Melatonin Up-Regulates Expression of CD62L by Lymphocytes in Aged Mice under Inflammatory and Non-Inflammatory Conditions.
Authors: Perfilyeva YV, Ostapchuk YO, Abdolla N, Tleulieva R, Krasnoshtanov VC, Belyaev NN Abstract It is well documented that age-related impaired functioning of immunocompetent cells is associated with an increase in the rates of chronic inflammatory diseases. Recently, an ability of melatonin to modulate inflammatory processes by regulating leucocyte recruitment has been demonstrated. However, to date, no studies have attempted to determine the impact of melatonin on the expression of CD62L by lymphocytes. CD62L, also known as L-selectin, is required for the entry of lymphocytes into secondary lymphoid organs, s...
Source: Immunological Investigations - March 21, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

MALAT1 Genomic Variants and Risk of Multiple Sclerosis.
Authors: Eftekharian MM, Noroozi R, Komaki A, Mazdeh M, Ghafouri-Fard S, Taheri M Abstract Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long noncoding RNA (lncRNA) with a possible role in the regulation of immune responses. A previous study has demonstrated down-regulation of this lncRNA in multiple sclerosis (MS) patients. In the current study, we genotyped two MALAT1 single nucleotide polymorphisms (SNPs) in 428 Iranian MS patients and 505 healthy subjects. The G allele of the rs619586 was significantly under-represented in MS patients compared with controls (OR (95% CI) = 0.65 (...
Source: Immunological Investigations - March 4, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Maternal Serum sEndoglin and Cell-Free Fetal DNA as Probable Markers of Preeclampsia: A Study in Single Center, Egypt.
This study was conducted to compare the levels of maternal serum soluble endoglin (sEng) and cell-free fetal DNA (cffDNA) in pregnant females with PE to normotensive pregnant ones, together with relating these levels to preeclampsia (PE) severity and onset. Method of the study: It was a comparative study in Mansoura University Hospital, Egypt, to detect the levels of serum sEng by ELISA besides the levels of cffDNA by quantitative real-time polymerase chain reaction in 80 pregnant females suffering from PE in addition to 80 normotensive pregnant ones that were included as control. RESULTS: Levels of serum sEng and cffD...
Source: Immunological Investigations - February 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Cloning and Analysis of the Multiple Transcriptomes of Serine Protease Homologs in Crayfish (Procambarus clarkii).
Authors: Zeng Y Abstract Five different serine protease homologs (SPH) transcripts presumably or possibly resulting from alternative splicing were cloned from the hemocytes of crayfish (Procambarus clarkii) in this paper. Although different deletions of cDNA of SPH-2 and SPH-4 were found in the 5' untranslated regions, they shared the same open reading frame and encoded a 424 amino acids protein with a calculated molecular weight of 45.84 kDa compared with SPH-5. The predicted cutting site of the signal peptide was located between Ala22 and Glu23; a clip domain and a trypsin-like serine protease domain were lo...
Source: Immunological Investigations - February 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Relative Expression of OX40, OX40L mRNA, and OX40L Serum Levels in Women with Recurrent Spontaneous Abortion.
In conclusion, our findings demonstrated that the expression of OX40 mRNA and OX40L mRNA was similar between women with a history of RSA and the control group. The elevation of serum OX40L level may be considered as a risk factor for RSA. PMID: 30794011 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - February 24, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

LPS-induced Apoptosis is Partially Mediated by Hydrogen Sulphide in RAW 264.7 Murine Macrophages.
Authors: George L, Ramasamy T, Sirajudeen K, Manickam V Abstract Lipopolysaccharide (LPS) induces apoptosis in murine macrophages through the autocrine secretion of tumor necrosis factor (TNF)-α and nitric oxide (NO). LPS-induced inflammation in murine macrophages is associated with hydrogen sulfide (H2S) production. In this present study, we reported the novel role of H2S in LPS-induced apoptosis and its underlying molecular mechanism specifically at late phases in murine macrophage cells. Stimulation of RAW 264.7 macrophages with LPS resulted in a time- and dose-dependent induction of apoptosis. We observed...
Source: Immunological Investigations - January 29, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Serum Levels of OPG, RANKL, and RANKL/OPG Ratio in Patients with Ankylosing Spondylitis: A Systematic Review and Meta-analysis.
CONCLUSIONS: Serum levels of OPG, RANKL, and RANKL/OPG ratio may be used as potential susceptible biomarkers for AS, but they could be influenced by race, inflammatory factors, and disease activity of AS patients. PMID: 30689477 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 29, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Neutrophil Functions in Immunodeficiency Due to DOCK8 Deficiency.
Authors: Mandola AB, Levy J, Nahum A, Hadad N, Levy R, Rylova A, Simon AJ, Lev A, Somech R, Broides A Abstract Neutrophil chemotactic defects have been reported previously in patients with hyper-IgE syndrome. Bi-allelic mutations in dedicator of cytokinesis 8 (DOCK8) gene usually cause an autosomal recessive hyper-IgE syndrome phenotype. Data are lacking about expression of DOCK8 protein in neutrophils or the possible role of DOCK8 in neutrophil function. We sought to determine if DOCK8 protein is expressed in neutrophils and if DOCK8 plays a role in neutrophil function. The expression of DOCK8 protein was assessed...
Source: Immunological Investigations - January 29, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Prognostic Values of CD38+CD101+PD1+CD8+ T Cells in Pancreatic Cancer.
In this study, we used flow cytometry and immunofluorescence assay to analyze the expression of CD38 and CD101 in 183 clinical PDAC samples, including 84 of peripheral blood and 99 of surgical tissues. High coexpression of CD38/CD101 on peripheral PD-1+CD8+ T cells or tumor-infiltrating lymphocytes (TILs) was found to be most significantly correlated with Tumor/Node/Metastasis (T/N/M) classification and clinical stage, in contrast PD-1+CD8+ T cells could not correlate with T classification. CD38/CD101 co-repression on TILs also correlated with the poor survival in these PDAC patient samples. Our data suggest that CD38/CD10...
Source: Immunological Investigations - January 29, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

TNF May Negatively Regulate Phagocytosis of Devil Facial Tumour Disease Cells by Activated Macrophages.
CONCLUSION: Our results indicate that TNF is not required for IFNγ and LPS or LPS alone activation of macrophage phagocytosis. TNF may negatively regulate macrophage phagocytosis of tumour cells. PMID: 30663448 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 23, 2019 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Effects of Soluble Tumor Necrosis Factor (TNF) on Antibody-Dependent Cellular Cytotoxicity of Therapeutic anti-TNF- α Antibody.
In this study, we established a new stable effector cell line expressing human FcγRIIIa, CD16:KHYG-1, and compared the performance of this cell line with that of peripheral blood mononuclear cells (PBMCs) in ADCC assays against CHO-derived target cells expressing protease-sensitive pro-TNF. Although an inhibitory effect of soluble TNF released from pro-TNF expressing cells on ADCC activity was seen, clear dose-responsive ADCC activities were observed even in the presence or absence of TNF-α converting enzyme (TACE) inhibitor. However, significant differences in the ADCC activities in the presence or absence of ...
Source: Immunological Investigations - December 22, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Decreased Gene Expression of Epstein-Barr Virus-Induced Gene 3 (EBI-3) may Contribute to the Pathogenesis of Rheumatoid Arthritis.
This study aims to determine the correlation between the gene expression of Epstein-Barr virus-induced gene 3 (EBI-3) and IL-12A (p35) subunits of IL-35 in peripheral blood leukocytes with immunological and clinical parameters in RA patients. METHODS: We recruited 47 patients with RA and 44 healthy subjects. The disease activity score-28 (DAS-28) was assessed by an expert rheumatologist and the plasma levels of neopterin and anti-cyclic citrullinated peptide (anti-CCP) was measured using ELISA method also Serum rheumatoid factor (RF) was assessed by the agglutination test. For the evaluation of IL-12A and EBI-3 gene ex...
Source: Immunological Investigations - December 6, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Immune Correlates of Protection Induced by Virus-Like Particles Containing 2009 H1N1 Pandemic Influenza HA, NA or M1 Proteins.
In this study, we generated VLPs containing hemagglutinin (HA), neuraminidase (NA) or M1 proteins derived from the A/California/04/09. Mice were immunized intramuscularly with HA-M1, NA-M1 or M1 VLPs and protective immunity was evaluated by assessing lung virus loads against low (5LD50) or high (100LD50) lethal dose of homologous virus challenges. High levels of virus-specific serum IgG antibody responses were induced in mice after HA-M1 VLPs immunization, whereas low or no IgG antibody responses were detected from immunization with NA-M1 VLPs or M1 VLPs, independently. Mice that were immunized with HA-M1 VLPs showed below...
Source: Immunological Investigations - November 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Characterization of Natural Killer Cells in HIV Patients Beginning Therapy with a High Burden of Cytomegalovirus.
CONCLUSION: A subpopulation of NK cells expressing NKG2C was induced by CMV replication in HIV patients heterozygous for a deletion in this gene. Individuals with an abundant NKG2C+ and LIR-1+ NK cells displayed lower levels of CMV reactive antibody. PMID: 30422022 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - November 15, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Body Mass Index and Mortality in Elderly Subjects from the Moli-Sani Study: A Possible Mediation by Low-Grade Inflammation?
Authors: Crotti G, Gianfagna F, Bonaccio M, Di Castelnuovo A, Costanzo S, Persichillo M, De Curtis A, Cerletti C, Donati MB, de Gaetano G, Iacoviello L, Moli-sani study Investigators Abstract The association between obesity and mortality in the elderly remains controversial. To test the association between BMI and mortality, with the hypothesis of a mediation by low-grade inflammation (LGI), a prospective study design (median follow-up 7.8 years) was used on a sample of 4,970 elderly subjects (age ≥ 65 years) from the Moli-sani Study cohort. The association between BMI categories and overall or cause-specific mo...
Source: Immunological Investigations - November 15, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

PD-1 Haplotype Combinations and Susceptibility of Patients to Squamous Cell Carcinomas of Head and Neck.
This study aimed to investigate the association of PD-1 gene polymorphisms at positions PD1.3 (rs11568821), PD1.5 (rs2227981) and PD1.9 (rs2227982) with susceptibility to HNSCCs. SUBJECTS AND METHODS: 150 patients pathologically confirmed to suffer from HNSCCs and 150 age-sex matched healthy controls were recruited in this study. Genomic DNA was extracted from white blood cells of all participants. Restricted fragment length polymorphisms (RFLP)-PCR was performed using site specific primers to determine the genotypes in each position. RESULTS: Statistical analyses indicated no significant differences in the frequen...
Source: Immunological Investigations - October 31, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Comparative Analysis of Bone Marrow-derived Mast Cell Differentiation in C57BL/6 and BALB/c Mice.
In this study, we prepared bone marrow-derived mast cells from BALB/c and C57BL/6 mice and examined their cellular properties. Cellular response to IL-3 and the process of mast cell differentiation from bone marrow cells were different on the basis of cell surface marker molecules. BALB/c-derived cells more efficiently exhibited degranulation than did C57BL/6-derived cells following both calcium ionophore and receptor crosslinking. These functional differences persisted even after a longer cell culture for 8 weeks, suggesting a difference in cell-autonomous characteristics. These results support the concept that mast ...
Source: Immunological Investigations - October 21, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Thyroglobulin Antibodies are Associated with Symptom Burden in Patients with Hashimoto's Thyroiditis: A Cross-Sectional Study.
CONCLUSIONS: Elevated TgAb levels are associated with symptom burden in HT patients, suggesting a role of thyroid autoimmunity in clinical manifestations of HT. Based on these results, we recommend screening for TgAb antibodies in HT patients with symptom burden. We also suggest that further work on understandings of symptoms appearance due to their autoimmune or hypothyroid causation is needed. PMID: 30332318 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 18, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Relationship of COX-2 Gene Polymorphisms and Susceptibility to Kawasaki Disease in Chinese Population.
Authors: Li S, Shi R, Tian L, Chen J, Li X, Huang L, Yang Z Abstract BACKGROUND: Kawasaki disease (KD) is an acute systemic vasculitis that predominantly affects children, and it can result in coronary artery lesions. Cyclooxygenase-2 (COX-2) is involved in the conversion of arachidonic acid to prostaglandin H2, an important precursor of several prostaglandins. The aim of this study was to examine the association between COX-2 gene polymorphisms and susceptibility to KD. METHODS: A total of 276 subjects (136 KD and 140 controls) were recruited. The analysis of two single nucleotide polymorphisms rs689466 (-1195...
Source: Immunological Investigations - October 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Adipokines in Multiple Sclerosis Patients with and without Optic Neuritis as the First Clinical Presentation.
CONCLUSIONS: MS patients with ON as the first manifestation display both lower disability scores and reduced serum adipokine levels implying that adipocytokine production is associated with MS progression. Exact mechanisms of this association in MS patients with first episode ON need to be further studied. PMID: 30321074 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Antigen-Presenting Cell Characteristics of Human γδ T Lymphocytes in Chronic Myeloid Leukemia.
In conclusion, the Vγ9Vδ2 T cells activated by cancer cell lysate showed APC characteristics, and this may greatly increase interest in investigating their therapeutic potential in hematologic malignancies. Abbreviations: CML: chronic myeloid leukemia; APC: antigen-presenting cell; TCR: T cell receptor; MHC: major histocompatibility complex; N-BPs: nitrogen-containing bisphosphonates; IPP: isopentenyl pyrophosphate; PBMC: peripheral blood mononuclear cells; NKG2D: natural killer receptor group 2, member D; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand. PMID: 30321079 [PubMed - as suppl...
Source: Immunological Investigations - October 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association between Toll-Like Receptor 3 (TLR3) rs3775290, TLR7 rs179008, TLR9 rs352140 and Chronic HCV.
Authors: Mosaad YM, Metwally SS, Farag RE, Lotfy ZF, AbdelTwab HE Abstract BACKGROUND: Inconsistent results were reported on the association of TLRs polymorphisms with the risk of HCV infection and HCV-related diseases. OBJECTIVE: to assess the relation between TLR3 rs3775290, TLR7 rs17900 and TLR9 rs352140 SNPs and chronic HCV in the Egyptian cohort and to study their relation to interferon response. METHODS: TLR3 rs3775290, TLR7 rs179008 and TLR9 rs352140 gene polymorphisms were typed by RFLP for 100 patients with chronic HCV and 25 with HCC in addition to 100 healthy controls. RESULTS: A significant ...
Source: Immunological Investigations - October 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Biological Markers of Oxidative Stress in Cardiovascular Diseases: After so Many Studies, What do We Know?
Authors: Fontana J, Zima M, Vetvicka V Abstract Numerous conditions, including cancer, diabetes, aging, and atherosclerosis, are thought to be associated with oxidative stress. Oxidative stress is defined as a persistent imbalance between oxidation and antioxidation, resulting in the damage of cellular macromolecules and is often considered to be involved in wide variety of human diseases. However, the current literature is very heterogeneous making it rather difficult to draw general conclusions. Often, different biomarkers have been used in different health problems. In addition, individual biomarkers are often m...
Source: Immunological Investigations - October 17, 2018 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research