Log in to search using one of your social media accounts:

 

Inconsistence between number and function of autoreactive T cells in the course of experimental autoimmune encephalomyelitis.
CONCLUSIONS: Both CD4(+) and CD8(+) T cells were most sensitive to MOG antigen stimulation for IFN-γ production during the early stage of EAE, but then rapidly lost the function despite their vigorous proliferation at the peak stage and later. PMID: 28872930 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Interleukin-4 and Interleukin-10 Gene Polymorphisms in Patients with Inflammatory Bowel Disease.
CONCLUSIONS: The present study suggests that IL-4 polymorphisms might play a role in susceptibility to IBD and its major subtypes in the Iranian population. PMID: 28872970 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Phenotypic and Functional Characteristics of Th17 (CD4(+)IL17A(+)) Cells in Human Oral Squamous Cell Carcinoma and Its Clinical Relevance.
Authors: Gaur P, Shukla NK, Das SN Abstract Recent studies have suggested an important role of T helper 17 (Th17) cells in tumor biology however, their phenotypic and functional aspects are poorly understood in context with oral cancer. We therefore, investigated the various phenotypic and functional markers of Th17 cells elucidating their relevance in oral squamous cell carcinoma (OSCC). Multi-color flow cytometry (FACs) was used to analyze the frequency and different markers of circulating Th17 cells ex vivo in peripheral blood mono-nuclear cells (PBMCs) from 69 OSCC patients and 35 healthy controls. Percent Mean...
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Expression and Significance of Th17 and Treg Cells in Pulmonary Infections with Gram-Negative Bacteria.
Authors: Liu Y, Sun JK, Qi X, Chen YM, Li J, Chen SY, Liu H Abstract The aim of this study was to investigate the expression and significance of T helper type 17 (Th17) and regulatory T (Treg) cells in severe pulmonary infection with gram-negative bacteria (GNB). The peripheral venous blood (PVB) and bronchoalveolar lavage fluid (BALF) were collected from patients receiving mechanical ventilation in the intensive care unit (ICU) owing to: (1) pulmonary GNB infection (group I) and (2) nonpulmonary infection (group NI). Patients from the two groups were matched based on their Acute Physiology and Chronic Health Evalu...
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Splenectomy-Induced Leukocytosis Promotes Intratumoral Accumulation of Myeloid-Derived Suppressor Cells, Angiogenesis and Metastasis.
CONCLUSION: Our data demonstrated a link among splenectomy-induced leukocytosis, accumulation of circulating and tumor-infiltrating MDSC, and enhanced angiogenesis and metastasis. Therefore, as a part of oncological surgery, favorable and unfavorable facets of the splenectomy must be considered to improve therapeutic efficacy. PMID: 28872973 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Postoperative Immunosuppressive Phenotypes of Peripheral T Helper Cells Are Associated with Poor Prognosis of Breast Cancer Patients.
CONCLUSIONS: The variations of peripheral T helper cell subsets showed postoperative immunosuppression and were associated with poor prognosis in IBC patients. PMID: 28872974 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Characterization of Inflammasome Components in Pig Intestine and Analysis of the Influence of Probiotic Enterococcus Faecium during an Escherichia Coli Challenge.
Authors: Kern M, Aschenbach JR, Tedin K, Pieper R, Loss H, Lodemann U Abstract The aim of the present study was to investigate systematically the expression of inflammasome components in pig intestine and to analyze the influence of age and long-term supplementation with the probiotic Enterococcus faecium NCIMB 10415 (E. faecium). In order to examine probiotic effects on the inflammasomes during a challenge with pathogens, enterotoxigenic Escherichia coli (ETEC) and E. faecium were directly added to pig jejunum in Ussing chambers. The mRNA expression of inflammasome components generally decreased in an oral-aboral ...
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Serum IL-6 and IL-8 Correlate with Prognostic Factors in Ovarian Cancer.
Authors: Sanguinete MMM, Oliveira PH, Martins-Filho A, Micheli DC, Tavares-Murta BM, Murta EFC, Nomelini RS Abstract The aim of the study was to correlate serum levels of IL-2, IL-5, IL-6, IL-8, IL-10, and TNF-α with clinical, laboratory, and pathological prognostic factors in patients with primary ovarian malignancy. Patients treated at the Pelvic Mass Ambulatory of the Discipline of Gynecology and Obstetrics/Oncology Research Institute (IPON) of the UFTM with confirmed diagnosis of malignant ovarian neoplasia (n = 26) were evaluated. Serum collection was performed preoperatively for the determinat...
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Growth Differentiation Factor-15 (GDF-15) Level and Relation to Clinical Manifestations in Egyptian Systemic Sclerosis patients: Preliminary Data.
CONCLUSION: GDF-15 serum levels were elevated in patients with SSc and correlated with the extent of skin fibrosis, and it was found to be higher in SSc patients with IPF. Such results may suggest a pivotal role of GDF-15 in fibrotic changes in SSc, and GDF-15 could be a treatment target in SSc patients in future. PMID: 28872977 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - September 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Expression and Clinical Signification of PD-1 in Lymph Nodes of Patients with Non-small Cell Lung Cancer.
Authors: Ma H, Mao G, Zhang G, Huang H Abstract To screen anti-programmed cell death protein 1 (PD-1) antibody treatment of the dominant population, it is necessary to understand the expression of PD-1 in tumor metastasis microenvironment. The aim of the present study was to detect the expression of PD-1 in lymph nodes of 51 patients with non-small cell lung cancer (NSCLC) by using flow cytometry (FCM). The results showed that the PD-1 expression on CD3(+) T cells was significantly increased in NSCLC metastatic lymph nodes (50.08 ± 8.03%) compared with nonmetastatic lymph nodes (36.25 ±...
Source: Immunological Investigations - August 12, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of Established Thyroid-stimulating Hormone and Free Thyroxine Genetic Variants with Hashimoto's Thyroiditis.
Authors: Brčić L, Gračan S, Barić A, Gunjača I, Torlak Lovrić V, Kolčić I, Zemunik T, Polašek O, Barbalić M, Punda A, Boraska Perica V Abstract Hashimoto's thyroiditis (HT), the most frequent autoimmune thyroid disease (AITD), is characterized by chronic inflammation of the thyroid gland that usually results in hypothyroidism. Thyroid-stimulating hormone (TSH) and free thyroxine (FT4) levels are used as clinical determinants of thyroid function. The main aim of this study was to explore the association of established TSH and FT4 genetic variants with HT. We performed a case-control analysis using 23...
Source: Immunological Investigations - July 29, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

IL-29 Enhances CXCL10 Production in TNF- α-stimulated Human Oral Epithelial Cells.
IL-29 Enhances CXCL10 Production in TNF-α-stimulated Human Oral Epithelial Cells. Immunol Invest. 2017 Aug;46(6):615-624 Authors: Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T Abstract Interleukin-29 (IL-29) is a cytokine belonging to the Type III interferon family. It was recently detected in the gingival crevicular fluid of periodontitis patients. However, the role of IL-29 in the pathogenesis of periodontal disease remains unknown. The aim of this study was to examine the effects of IL-29 on C-X-C motif chemokine ligand 10 (CXCL10) production in human oral epithelial cells. We measured C...
Source: Immunological Investigations - July 29, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Role of Different CD40 Polymorphisms in Graves' Disease and Hashimoto's Thyroiditis.
The objective of this study was to identify the relationship of the polymorphisms of three sites of CD40 with the susceptibility to AITD in the Chinese population. We genotyped three polymorphisms of CD40: C/T -1 SNP, 58038T site of the third exon and C64610G site of the ninth exon in 196 GD cases, 121 HT cases and 122 control subjects. The three putative polymorphism sites were amplified by PCR for sequencing and analysis. The genotype frequencies of CD40 -1 C/C genotype and C allele were significantly higher in the GD group than those in normal control. For the C64610G polymorphism, the C/G genotype was significantly mor...
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Chikungunya Virus Seroprevalence and Associated Factors among Hospital Attendees in Two States of Southwest Nigeria: A Preliminary Assessment.
Authors: Olajiga OM, Adesoye OE, Emilolorun AP, Adeyemi AJ, Adeyefa EO, Aderibigbe IA, Adejumo SA, Adebimpe WO, Opaleye OO, Sule WF, Oluwayelu DO Abstract Chikungunya virus (CHIKV) is a re-emerging pathogen causing long-term polyarthritis and encephalitis. In conducting a preliminary investigation, we hypothesized that there is no serologic evidence of CHIKV infection among attendees of selected hospitals in Lagos and Osun States, Nigeria. Sera from 304 consecutively selected participants were screened for CHIKV IgG and IgM using ELISA. Findings were analyzed vis-à-vis participants' demographic and clinical ...
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of IL-10-Regulating MicroRNAs in Peripheral Blood Mononuclear Cells with the Pathogenesis of Autoimmune Thyroid Disease.
In conclusion, the expression levels of hsa-miR-106a-5p and hsa-miR-223-3p were associated with the pathogenesis of AITDs. hsa-miR-98-5p may negatively regulate the expression of IL-10. The functional polymorphism of MIR27A was associated with HD severity. PMID: 28742402 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Pregnane X Receptor Polymorphisms and Risk of Inflammatory Bowel Disease: A Meta-Analysis.
CONCLUSION: Our meta-analysis revealed that PXR gene polymorphism may not be significantly associated with IBD susceptibility. However, the number of original studies was limited and further studies with large samples are needed to verify the results. ABBREVIATIONS: PXR = pregnane X receptor, IBD = inflammatory bowel disease, CD = Crohn's disease, UC = ulcerative colitis, ORs = pooled odds ratios, 95% CIs = 95% confidence intervals, NOS = Newcastle-Ottawa scale, HWE = Hardy-Weinberg equilibrium. PMID: 28742404 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

TLR5 Polymorphisms rs2072493, rs5744174, and rs5744168 Are Not Genetic Risk Factors for Chronic Helicobacter pylori Infection in Indian Tamils.
Authors: Goda V, Jayaraman M, Loganathan R, Nazeer M, Ali M, Karunakaran P, Devaraju P Abstract The incidence of Helicobacter pylori (H. pylori) infection and gastric cancer is on the rise in India, and the genetic factors influencing the increased susceptibility in Indian population remain obscure. Toll-like receptors (TLRs) play a major role in innate immune system and genetic polymorphisms affecting their function were reported to enhance the risk for H. pylori infection. Seventy-seven patients (n = 77) diagnosed with H. pylori infection and 230 healthy subjects were recruited in this study. The rs2072493, rs574...
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Air- and Dust-Borne Fungi in Indoor and Outdoor Home of Allergic Patients in a Dust-Storm-Affected Area.
Authors: Shabankarehfard E, Ostovar A, Farrokhi S, Naeimi B, Zaeri S, Nazmara S, Keshtkar M, Sadeghzadeh F, Dobaradaran S Abstract Despite the recent increases in fungi-induced allergic diseases, there is no report yet in the region of the Persian Gulf on concentration levels of fungi in relationship with health state. Therefore, our aim was to measure fungi prevalence as well as to evaluate the relationships between air- and dust-borne fungal genera and allergic diseases. A matched case-control study was carried out including 45 allergic cases and 45 age- and gender-matched controls for each individual. Indoor and...
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

MICA-129Met/Val Polymorphism Is Associated with Early-Onset Breast Cancer Risk.
In this study, we investigated the potential interaction between genetic polymorphism of MICA and the development of breast cancer. We recruited 192 unrelated Tunisian women affected by breast cancer and 205 controls age-matched women, all genotyped for MICA-129 Met/Val (rs 1051792). A significant association was found between the Val allele and Val/Val genotype and the risk of breast cancer (p = 0.002, OR = 1.64, 95% CI = [1.17-2.27]; p = 0.002, OR = 1.88, 95% CI = [1.24-2.87], respectively). After stratification with clinical-pathology parameters, we found ...
Source: Immunological Investigations - July 26, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of PD-1.5 C/T, but Not PD-1.3 G/A, with Malignant and Benign Brain Tumors in Iranian Patients.
In conclusion, we demonstrate that PD-1.5 C/T polymorphism, but not PD-1.3 G/A, is associated with brain tumors in Iranian patients. PMID: 28535114 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - May 24, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Th17-Inducing Conditions Lead to in vitro Activation of Both Th17 and Th1 Responses in Behcet's Disease.
Authors: Deniz R, Tulunay-Virlan A, Ture Ozdemir F, Unal AU, Ozen G, Alibaz-Oner F, Aydin-Tatli I, Mumcu G, Ergun T, Direskeneli H Abstract OBJECTIVES: Interleukin-17 (IL-17) has been associated with the pathogenesis of various autoimmune/inflammatory diseases. The aim of this study was to investigate the expression of Th17-related immunity in an innate immunity-dominated vasculitis, namely Behcet's disease (BD). METHODS: Peripheral blood mononuclear cells from 37 patients (age: 38.5 ± 9.8 years) with BD, and 25 healthy controls (HC) (age: 39.1 ± 9.3 years), were cult...
Source: Immunological Investigations - April 18, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Progesterone Decreases in vitro Indoleamine 2, 3-dioxygenase Expression in Dendritic and CD4(+) Cells from Maternal-Fetal Interface of Rats.
CONCLUSION: The blocking of progesterone receptor on these cells by mifepristone restored IDO expression levels and may constitute evidence of the participation of this hormone through a direct route in these cells. PMID: 28414589 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 18, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Reduction in CD16/CD56 and CD16/CD3/CD56 Natural Killer Cells in Coronary Artery Disease.
Authors: Jabir NR, Firoz CK, Ahmed F, Kamal MA, Hindawi S, Damanhouri GA, Almehdar HA, Tabrez S Abstract BACKGROUND: Natural killer (NK) cells are the potential modulators of inflammatory reactions that exert several unique biological effects and could lead to future adverse events of coronary artery disease (CAD). HYPOTHESIS: The purpose of this study was to find out the possible association of modulation in NK cell, TNK cells, T cells, B cells, and tumor necrosis factor alpha (TNF-α) in CAD patients and various forms of myocardial infarction. METHODS: The present study included total 190 subjects (9...
Source: Immunological Investigations - April 18, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Variable Heavy Chain Domain Derived from a Cell-Penetrating Anti-DNA Monoclonal Antibody for the Intracellular Delivery of Biomolecules.
In this study, we explored the applicability of variable heavy chain (VH) domain as delivery vehicles for mammalian cells. The characteristics of the recombinant VH domain produced from a cell-penetrating monoclonal anti-double stranded DNA antibody 2C10 were analyzed using flow cytometry, confocal microscopy, cell proliferation assay, and cell cycle analysis in various mammalian cell lines. The VH domain penetrated into various cell lines in a time- and dose-dependent manner, although the internalization efficiency varied. The domain was localized in the nuclei as well as the cytoplasm of living cells. It was also interna...
Source: Immunological Investigations - April 8, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Natural Killer Cell Subsets and Their Functional Activity in Beh çet's Disease.
CONCLUSION: Increases in NK1/NK2 ratio and CD16(+)IFN-γ(+) NK1 cells might support the idea of a biased IFN-γ dominant immune response in the mucocutaneous involvement of BD pathogenesis. Although the cytokine secretion pattern was different, no difference was obtained in cytotoxic activity, expression of activatory receptors, or degranulation of NK cells. PMID: 28388249 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 8, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Matrix Metalloproteinase-9 rs17576 Gene Polymorphism and Beh çet's Disease: Is There an Association?
CONCLUSION: our study suggests a significant association of the MMP-9 rs17576 A/G polymorphism with increased risk of BD development in Egyptian patients. PMID: 28388268 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 8, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Investigation of AID, Dicer, and Drosha Expressions in Patients with Chronic Lymphocytic Leukemia.
In this study, whether the changes of AID, Dicer and Drosha expressions may be associated with both deletions and clinical outcomes in patients with CLL were investigated. AID expressions were increased in patients with CLL. However, cell lysate AID protein levels were only increased in patients with del17p or del11q who have poor prognosis. Decreased Dicer expressions were found in patients with deletion, whereas increased Drosha expressions were found in patients without deletion and with del13q. According to Rai and Binet staging systems, advanced-stage patients showed increased AID protein levels, decreased Dicer and D...
Source: Immunological Investigations - April 8, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Influence of the Co-Administration of Heptavalent Conjugate Vaccine PCV7-TT on the Immunological Response Elicited by VA-MENGOC-BC ® and Heberpenta®-L in Rabbits.
Influence of the Co-Administration of Heptavalent Conjugate Vaccine PCV7-TT on the Immunological Response Elicited by VA-MENGOC-BC® and Heberpenta®-L in Rabbits. Immunol Invest. 2017 Apr 05;:1-14 Authors: Espinosa-Viñals C, García-Rivera D, Rodríguez Noda L, Amador Gómez A, Nicot M, Valle O, Núñez JF, Martin Y, Santana D, Valdés Y, Vérez Bencomo V Abstract Finlay Vaccine Institute is developing a new heptavalent conjugate vaccine against Streptococcus pneumoniae. As infants are the target population, PCV7-TT will be necessarily co-administ...
Source: Immunological Investigations - April 7, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Inflammatory CXC Chemokines, GRO α(high), IP-10(low), and MIG(low), in Tumor Microenvironment Can Be Used as New Indicators for Non-small Cell Lung Cancer Progression.
CONCLUSION: In summary, tumor tissue-infiltrating CXC chemokines, GROα(high), IP-10(low), and MIG(low) in the tumor microenvironment can be used as potential indicators for the progression of NSCLC. PMID: 28375674 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 5, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

High Interleukin-10 Expression in Type 2 T Helper Cells in Septic Patients.
In conclusion, the production of IL-10 in Th2 cells was higher in the patients with sepsis. PMID: 28375710 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 5, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Immunization with Recombinantly Expressed LRP4 Induces Experimental Autoimmune Myasthenia Gravis in C57BL/6 Mice.
CONCLUSION: Our results confirm the EAMG-inducing action of LRP4 immunization and identify B6 as a LRP4-EAMG-susceptible mouse strain. Demonstration of complement fixing anti-LRP4 antibodies in sera and complement/IgG deposits at the NMJ of LRP4-immunized mice indicates complement activation as a putative pathogenic mechanism. We have thus developed a practical LRP4-induced EAMG model using a non-conformational protein and a widely available mouse strain for future investigation of LRP4-related MG. PMID: 28375749 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 5, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Differential Expression of Toll-Like Receptor Signaling Pathway Is Associated with Microscopic Polyangiitis in Peripheral Blood Neutrophils.
Authors: Lai Y, Xue C, Liao Y, Huang L, Peng Q, Huang B, Wei S, He L, Gong A, Wang M Abstract Constitutive or excessive activation of Toll-like receptor (TLR) signaling pathway can disrupt the body's immune tolerance to autoantigen, thus promoting the development of autoimmune disease. However, the expression profile of TLR signaling pathway in peripheral blood neutrophils in the pathogenesis of microscopic polyangiitis (MPA) remains unclear. Thus, improved understanding of the pathobiology of this disease may aid in the development of therapeutic targets for patients with MPA. In the present study, we assessed the...
Source: Immunological Investigations - April 5, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

PTPN22 Single-Nucleotide Polymorphisms in Iranian Patients with Type 1 Diabetes Mellitus.
CONCLUSIONS: The PTPN22 rs2476601 minor allele (A) was associated with T1D in Iran, accounting for its pathophysiology in autoimmune diseases. PMID: 28375784 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 5, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Effect of Surgical Stress on Postoperative T αβ and Tγδ Cell Distribution.
CONCLUSION: Surgery results in decreased percentage of Tγδ lymphocytes in peripheral human blood, and this correlates with the severity and location of the surgical trauma. This observation may help to predict postoperative recovery after gastroabdominal surgery. PMID: 28358227 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 1, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Measurement of Health-Related Quality of Life in Primary Antibody-Deficient Patients.
CONCLUSIONS: PAD patients had significantly lower scores in mental and physical components compared to normal population. By early diagnosis and long-term follow-up periods, we may be able to prevent complications and help patients to have a better QoL. PMID: 28358233 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - April 1, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Changes in the Level of Immunoglobulins and CD4/CD8 Ratio in Young and Aged Mice with Estradiol Deficiency.
Authors: Shao MJ, Zhu YJ, Qiu YE, Hu M, He YQ Abstract Studies demonstrated that deficiency in 17β-estradiol (E2) in postmenopausal women influences their immune system. However, few studies have reported alterations in immunologic presentation during nonnatural menopause in young females. Here we compared the differences in immune response between young C57BL/6N mice with surgical or medical variectomy and aged C57BL/6N mice with the common feature of E2 deficiency following Con A stimulation. We observed inverted CD4/CD8 ratios in the aged group and apparent reduced production of serum immunoglobin (Ig)G, Ig...
Source: Immunological Investigations - March 25, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Immunomodulating and Immunoresistance Properties of Cancer-Initiating Cells: Implications for the Clinical Success of Immunotherapy.
Authors: Maccalli C, Parmiani G, Ferrone S Abstract Cancer-initiating cells (CICs) represent a relatively rare subpopulation of cells endowed with self-renewal, stemness properties, tumorigenicity in immunodeficient mice, and resistance to standard therapies as well as to immunotherapy. Here, we review the biological and immunological characteristics of CICs with special focus on the immunomodulating mechanisms they utilize to escape from immunosurveillance. The recently developed immunotherapeutic strategies have yielded remarkable clinical results in many types of tumors, indicating that indeed a patient's immune...
Source: Immunological Investigations - March 14, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Assessment of Immunomodulatory Activities and in vitro Toxicity of New Quinolone 7-ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo[3,4-h]quinoline-7-carboxylate.
Authors: Jantová S, Paulovičová E, Paulovičová L, Topoľská D, Pánik M, Milata V Abstract Our previous studies on leukemia cells L1210 and cervical cancer HeLa cells revealed cytotoxic effects of the 7-ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo[3,4-h]quinoline-7-carboxylate (E2h), a new synthetically prepared quinolone derivative, toward selected cancer cell lines. The aim of the present study was to examine the cytotoxicity of E2h toward next cell lines and tissues; that is, human cancer HL-60 and A549 cells, human non-cancer fibroblast BHNF-1 cells, and reconstructed ...
Source: Immunological Investigations - March 14, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Association of Interleukin-16 Gene Polymorphisms with IL-16 Serum Levels and Risk of Multiple Sclerosis.
Authors: Farrokhi M, Masoudifar A, Derakhshan A, Saadatmand S, Rouhi-Boroujeni H, Etemadifar M, Rezaei-Zarji S, Javid A, Nobakht R, Deyhimi M, Ekramnia A, Ebrahimi M, Sheikh S, Ansaripour S, Amani-Beni A, Jahanbani-Ardakani H Abstract BACKGROUND: Multiple sclerosis (MS) is a chronic inflammatory, demyelinating, and neurodegenerative disease of the central nervous system (CNS) that is immunologically mediated in genetically susceptible individuals. Interleukin-16 (IL-16), a pleiotropic cytokine, is an important regulator of T-cell activation which plays a key role in autoimmune diseases. Single-nucleotide polymorphi...
Source: Immunological Investigations - February 4, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Graft Immunocomplex Capture Fluorescence Analysis to Detect Donor-Specific Antibodies and HLA Antigen Complexes in the Allograft.
CONCLUSIONS: This novel application would detect early phase or incomplete pathological cases of AMR, which could lead to a correct diagnosis and initiation of appropriate therapies. Moreover, graft ICFA might address a variety of long-standing questions in terms of DSA. ABBREVIATIONS: AMR: Antibody-mediated rejection; DSA: Donor-specific antibodies; ICFA: Immunocomplex capture fluorescence analysis. PMID: 28151033 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - February 4, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

sFRP3 and DKK1 Regulate Fibroblast-Like Synoviocytes Markers and Wnt Elements Expression Depending on Cellular Context.
CONCLUSION: Our results confirm the involvement of Wnt signaling in FLS transformation and show that two inhibitors of the same cascade can regulate differently the same elements and that a single inhibitor can initiate signaling depending on cellular context. ABBREVIATIONS: FLS: fibroblast-like synoviocytes; RA: rheumatoid arthritis; Wnt: Wingless; Fz: frizzled; LRP: Fz/low-density lipoprotein receptor protein; WISP1: Wnt1 inducible signaling pathway protein 1; sFRP: secreted Fz-related proteins; DKK: Dickkopf; OA: osteoarthritis; DMEM: Dulbecco's modified Eagle's medium; FBS: fetal bovine serum; PBS: phosphate buffer...
Source: Immunological Investigations - February 4, 2017 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Meta-analysis of the Relationship between TNF- α (-308G/A) and IL-10 (-819C/T) Gene Polymorphisms and Susceptibility to Dengue.
CONCLUSION: This meta-analysis showed that TNF-α (-308) polymorphism is associated with dengue symptomatic susceptibility. PMID: 27982730 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - December 17, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

IL-37 a New IL-1 Family Member Emerges as a Key Suppressor of Asthma Mediated by Mast Cells.
Authors: Conti P, Ronconi G, Caraffa A, Lessiani G, Duraisamy K Abstract In 1986, we reported a multiple biological effect of IL-1 including immunological, inflammatory, and tumor killing activity. Since then other IL-1 family cytokines have been discovered, some with inflammatory and other with anti-inflammatory activity. In this review article, we speculate on the possible inhibitory effect of IL-37 in the light of new findings. IL-37, formerly termed IL-1 family member 7 (IL-1F7), binding IL-18 receptor α chain, acts as a cytokine with intracellular as well as extracellular functionality and as a natural i...
Source: Immunological Investigations - December 17, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) Levels and Its Association with Renal Allograft Rejection.
This study was conducted to access the role of urinary CCL2 expression in predicting the rejection episodes in renal transplant patients. METHOD: A total of 409 urine samples included in this study. The samples were consisted of (a) biopsy-proven graft rejection (n = 165); (b) non-rejection (n = 93); (c) non-biopsy stable-graft (n = 42), and (d) healthy renal donors (n = 109). The samples were quantified for the CCL2 using the MCP-1/CCL2 ELISA kit. The data were analyzed using the Statistical Package for Social Sciences (SPSS(®)) and MedCalc(®) statistical software. RESULTS: Results showed that the CCL2 lev...
Source: Immunological Investigations - December 16, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Vinpocetine Inhibited the CpG Oligodeoxynucleotide-induced Immune Response in Plasmacytoid Dendritic Cells.
In this study, we evaluated the effect of vinpocetine on phenotype of pDCs isolated from C57BL/6 mice and explored its possible mechanism. Our data showed that vinpocetine significantly downregulated the expression of CD40, CD80, and CD86 on pDCs and increased the expression of translocator protein (TSPO), the specific receptor of vinpocetine, in pDCs. Vinpocetine significantly inhibited the Toll-like receptor 9 signaling pathway and reduced the secretion of related cytokines in pDCs through TSPO. Furthermore, viability of pDCs was significantly promoted by vinpocetine. These findings imply that vinpocetine serves as an im...
Source: Immunological Investigations - December 16, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Tamoxifen Attenuates Lipopolysaccharide/Galactosamine-induced Acute Liver Failure by Antagonizing Hepatic Inflammation and Apoptosis.
Authors: Zhang P, Zhang M, Wan M, Huang X, Jiang Y, Xu S, Luo M Abstract Bacterial lipopolysaccharide (LPS)-induced acute liver failure (ALF) is a common severe clinical syndrome in intensive care unit. No other methods are available for its prevention apart from supportive treatment and liver transplantation. Tamoxifen (TAM) was reported to attenuate ALF induced by excessive acetaminophen, while its effect on LPS-induced ALF remained unknown. For this, in the present study, we comprehensively assessed whether TAM can attenuate ALF induced by LPS/galactosamine (GaIN). Mice were given TAM once a day for three times....
Source: Immunological Investigations - December 16, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Local and Systemic Profiles of Inflammatory Cytokines in Carrageenan-induced Paw Inflammation in Rats.
In this study, we aimed to investigate the local and systemic expression profiles of various inflammatory cytokines following the subplantar injection of CA in rats. METHODOLOGY: Acute inflammation was induced in male Wistar rats by subplantar injection of CA. Serum and paw tissue were examined for the level of 19 specific inflammatory cytokines using antibody array. Further, the CA-elicited level of key inflammatory cytokines, cytokine-induced neutrophil chemoattractant (CINC)-2, CINC-3, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, were quantified by enzyme-linked immunosorbent assay (ELISA...
Source: Immunological Investigations - December 16, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Anti-CD14 Antibody-treated Neutrophils Respond to LPS: Possible Involvement of CD14 Upregulated by Anti-CD14 Antibody Binding.
Authors: Sanui T, Takeshita M, Fukuda T, Haraguchi A, Aida Y, Nishimura F Abstract CD14 and Toll-like receptor 4/MD2 (TLR4/MD2) mediate the action of LPS on neutrophils. The anti-CD14 antibody and the TLR4/MD2-antagonist, synthetic lipid IVa (LA-14-PP), are known to inhibit the response of neutrophils to LPS. We studied the role of CD14 in LPS-induced priming of neutrophils for enhanced release of the superoxide anion. The anti-CD14 antibody at much higher concentrations than required to saturate CD14 was required to inhibit priming by LPS. The inhibitory effect of the anti-CD14 antibody was overcome by LPS. After ...
Source: Immunological Investigations - December 4, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Upregulation of T-cell Immunoglobulin and Mucin-Domain Containing-3 (Tim-3) in Monocytes/Macrophages Associates with Gastric Cancer Progression.
In conclusion, our study demonstrated that Tim-3 expressed by monocyte/macrophages might be an important mechanism in gastric cancer progression. PMID: 27911104 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - December 4, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Mannose-Binding Lectin Deficiency in Brazilian Patients with Spondyloarthritis.
Authors: Skare TL, Nisihara R, Cieslinski JZ, Zeni JO, Rasera HN, Messias-Reason I, Utiyama SR Abstract BACKGROUND: Infections are usually involved in the pathogenesis of spondyloarthritis (SpA). Mannose-binding lectin (MBL) is a component of the innate immune system with an important role in microbial defense. OBJECTIVE: To study the prevalence of MBL deficiency in SpA patients as well as its influence in the clinical profile of these diseases. METHODS: We studied 89 SpA patients and 89 healthy individuals, paired for age and gender. MBL serum levels were measured by ELISA test. Individuals with levels &le...
Source: Immunological Investigations - December 4, 2016 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research