Trehalose and N-Acetyl Cysteine Alleviate Inflammatory Cytokine Production and Oxidative Stress in LPS-Stimulated Human Peripheral Blood Mononuclear Cells
Immunol Invest. 2021 Feb 25:1-17. doi: 10.1080/08820139.2021.1891095. Online ahead of print.ABSTRACTBackground: Evidence has shown that inflammation and oxidative stress are implicated in the development of a great number of human diseases. Trehalose possesses various biological effects including antioxidant and anti-inflammatory activities. However, there is little data on the effects of trehalose on human cells including peripheral blood mononuclear cells (PBMCs). Here, we aimed to investigate whether trehalose could attenuate oxidative stress and inflammation induced by lipopolysaccharides (LPS) in PBMCs.Methods: The en...
Source: Immunological Investigations - February 26, 2021 Category: Allergy & Immunology Authors: Ali Reza Bastin Mahdieh Nazari-Robati Hossein Sadeghi Amir Hossein Doustimotlagh Asie Sadeghi Source Type: research

Application of Emerging Plant-Derived Nanoparticles as a Novel Approach for Nano-Drug Delivery Systems
Immunol Invest. 2021 Feb 25:1-21. doi: 10.1080/08820139.2021.1891094. Online ahead of print.ABSTRACTNanotechnology has enabled the delivery of small molecular drugs packaged in nanosized vesicles to the target tissues. Plant-Derived Nanoparticles (PDNPs) are vesicles with natural origin and unique properties. These nanoparticles have several advantages over synthetic exosomes and liposomes. They provide bioavailability and biodistribution of therapeutic agents when delivered into different tissues. These nanoparticles can be modified according to the specificity of their functions in target tissues. When PDNPs are internal...
Source: Immunological Investigations - February 25, 2021 Category: Allergy & Immunology Authors: Parisa Sarvarian Parisa Samadi Elham Gholipour Karim Shams Asenjan Mohammad Hojjat-Farsangi Roza Motavalli Farhad Motavalli Khiavi Mehdi Yousefi Source Type: research

Role of miR-146a rs2910164 and UTS2 rs228648 Genetic Variants in Beh çet's Disease.
Conclusion: Our results suggest that miR-146a rs2910164 and UTS2 rs228648 variants have significant roles in both the development and clinical modulation of BD in Egyptian patients. PMID: 33605830 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - February 20, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Buserelin Inhibits the Immunosuppressive Activity of Regulatory T Cells through the Protein Kinase A Signaling in a Central Precocious Puberty Model.
Conclusion: Buserelin impaired the immunosuppressive activity of Tregs through the PKA signal pathway. Buserelin-induced activation of PKA signaling down-regulated Foxp3 expression while promoting RORγt expression in Tregs, and subsequently weakened Treg function. Our study indicates the necessity of monitoring Treg activity in CPP patients to avoid potential autoimmunity or inflammation. PMID: 33586576 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - February 17, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The increased risk of atrial fibrillation in inflammatory arthritis: a systematic review and meta-analysis of cohort studies.
Authors: Ma Y, Pan Z, Fan D, Xu S, Pan F Abstract Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia contributing to stroke and sudden cardiac death. Numbers of studies indicated that patients with inflammatory arthritis have an increased risk of AF. The present study aims to assess the risk of AF in inflammatory arthritis patients. Methods: We systematically searched cohort studies regarding the risk of AF in patients with rheumatoid arthritis, or spondyloarthritis through PubMed, Web of Science, Cochrane Library, Clinical Trials Registry, and China National Knowledge from inception to Augu...
Source: Immunological Investigations - February 12, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Associations of Tumor Necrosis Factor Alpha Gene Polymorphisms and Ankylosing Spondylitis Susceptibility: A Meta-analysis Based on 35 Case-control Studies.
Authors: Gao S, Liang W, Xu T, Xun C, Cao R, Deng Q, Zhang J, Sheng W Abstract Background: Scores of studies on tumor necrosis factor alpha (TNF-α) gene polymorphisms and AS have been performed with inconsistent results. The purpose of this study was to provide some more convincing evidence on the associations of TNF-a polymorphisms and AS by using a meta-analysis approach. Methods: Potentially relevant studies were identified from Web of Science, PubMed, EMBASE, Wanfang, and CNKI from inception to March 5, 2020. Newcastle-Ottawa Scale (NOS) was utilized to appraise the quality of included studies. Odds ratio...
Source: Immunological Investigations - February 10, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Interleukin-10 Levels are Associated with Doxorubicin-Related Cardiotoxicity in Breast Cancer Patients in a One-Year Follow-Up Study.
This study aimed to assess changes in plasma levels of interleukin (IL)-1β, IL-6, IL-10 and tumor necrosis factor (TNF) after chemotherapy and to correlate these levels with cardiac biomarkers and clinical data. Methods: Sixty-four patients with breast cancer treated with DOXO were included. Twenty-two subjects (cases) developed cardiotoxicity until one year after the end of DOXO treatment. Cytokines and cardiac markers were evaluated before starting chemotherapy (T0), up to 7 days after the last infusion (T1) and 12 months after the last infusion (T2). Results: Higher IL-10 levels were observed in the case ...
Source: Immunological Investigations - February 10, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Age-associated Ligand-receptor Interactions Imputed from Nasopharyngeal Transcriptomes of COVID-19 Patients.
Authors: Batchu S, Yu S Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has exhibited different clinical manifestations amongst various age cohorts. As the immune microenvironment may play a role in clinical progression, it is crucial to examine molecular interactions to gain insight into host response. Therefore, to elucidate any differences in host response related to age, the present study imputed ligand-receptor interactions within the nasopharyngeal immune microenvironment in patients affected with SARS-COV-2. Tissue purities, the proportion of non-immune cells in the tissue sample, of 46...
Source: Immunological Investigations - February 4, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Effect of Soluble TREM-1 in Idiopathic Granulomatous Mastitis.
Authors: Ates D, Doner HC, Kurban S, Koksal H Abstract Background and objectives: The aim of this study is to investigate the effect of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in idiopathic granulomatous mastitis (IGM). Methods: This case-control study was conducted in Saglik Bilimleri and Necmettin Erbakan Universities. Sixty patients with IGM diagnosis (Group P) and 25 healthy females as control group (Group C) were included. Group P was divided into two subgroups according to the activity of disease: patients with active lesion (Group PA), and patients without any symptoms, in remissio...
Source: Immunological Investigations - February 3, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

IL18-family Genes Polymorphism Is Associated with the Risk of Myocardial Infarction and IL18 Concentration in Patients with Coronary Artery Disease.
Conclusions: Thus, polymorphism in the genes of IL18 receptor is determine the IL18 contents and important in the development of coronary atherosclerosis, associated pathological conditions and the risk of acute coronary events; prospective monitoring of patients with early clinical signs of adverse events is required to confirm the role of IL18, IL18R1, and IL18RAP genes polymorphism in atherogenesis. PMID: 33522333 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - February 3, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Role of the IL-33/ST2 Immune Pathway in Autoimmunity: New Insights and Perspectives.
Authors: Ramezani F, Babaie F, Aslani S, Hemmatzadeh M, Mohammadi FS, Gowhari-Shabgah A, Jadidi-Niaragh F, Ezzatifar F, Mohammadi H Abstract Interleukin (IL)-33, a member of IL-1 cytokine family, is produced by various immune cells and acts as an alarm to alert the immune system after epithelial or endothelial cell damage during cell necrosis, infection, stress, and trauma. The biological functions of IL-33 largely depend on its ligation to the corresponding receptor, suppression of tumorigenicity 2 (ST2). The pathogenic roles of this cytokine have been implicated in several disorders, including allergic disease, c...
Source: Immunological Investigations - February 3, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Overlap between Genetic Susceptibility to COVID-19 and Skin Diseases.
Authors: Jabalameli N, Rajabi F, Firooz A, Rezaei N Abstract Coronavirus disease 2019 (COVID-19) mainly affects the respiratory system, but the involvement of other organ systems has also been commonly reported. Acute acro-ischemia or chilblain like lesions were among the first recognized dermatological presentations of COVID-19. Though the occurrence of such lesions has been attributed to the similar interferon-1 mediated immune response in both COVID-19 and systemic lupus erythematosus, we propose another possible explanation based on a common genetic background. In a recent genome-wide association study, the 3p2...
Source: Immunological Investigations - January 28, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Expression and Role of microRNA-133a in Plasma of Patients with Kawasaki Disease.
Authors: Luo Y, Yu M, Li P, Huang L, Wu J, Kong M, Li Y, Wu Z, Kang Z, Yi L, Yang Z Abstract Kawasaki disease (KD)), also known as mucocutaneous lymph node syndrome (MCLS), is an autoimmune and systemic vasculitis syndrome. Its etiology and pathogenesis are still unclear. microRNAs (miRNA), a novel class of small non-coding RNAs, regulate the expression of multiple protein-encoding genes at the post-transcriptional level. We intend to study the change of miRNA-133a in the plasma of patients with KD, explore the role of miRNA-133a on HUVEC and define the pathogenesis of vascular dysfunction in KD. miRNA-133a ex...
Source: Immunological Investigations - January 28, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Long Term Follow-Up of the Patients with Severe Combined Immunodeficiency After Hematopoietic Stem Cell Transplantation: A Single-Center Study.
Authors: Demirtas D, Cagdas D, Turul Ozgur T, Kuskonmaz B, Uckan Cetinkaya D, Sanal O, Tezcan I Abstract Background: We aimed to evaluate hematopoietic stem cell transplantation (HSCT) related outcomes of patients with severe combined immunodeficiency (SCID). Methods: We retrospectively collected data from SCID patients who were diagnosed, followed up and survived at least 2 years after HSCT. Results: Forty four SCID patients were included in the study. Median age of HSCT and follow-up period after HSCT were 7.1 months and 8.7 years, respectively. Human leukocyte antigen (HLA) identical donors were used i...
Source: Immunological Investigations - January 23, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association between IL-37 and Systemic Lupus Erythematosus Risk.
Authors: Wu Q, Zhou J, Yuan ZC, Lan YY, Xu WD, Huang AF Abstract Interleukin-37 (IL-37) is an anti-inflammatory cytokine. In our former study, we found increased plasma IL-37 levels in systemic lupus erythematosus (SLE) patients. However, relationship between IL-37 levels and clinical laboratory characteristics of SLE patients has not been elucidated. In addition, association of IL37 gene polymorphism with SLE risk needs to be discussed. A group of 580 individuals (220 SLE patients and 360 healthy controls) in a Southern Chinese Han population were recruited. Plasma IL-37 levels were evaluated using enzyme-linked i...
Source: Immunological Investigations - January 21, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

miR-558 Reduces the Damage of HBE Cells Exposed to Cigarette Smoke Extract by Targeting TNFRSF1A and Inactivating TAK1/MAPK/NF- κB Pathway.
CONCLUSIONS: Up-regulation of miR-558 relieved the damage of HBE cells-triggered by CSE via reducing TNFRSF1A and inactivating TAK1/MAPK/NF-κB pathway, affording novel molecules for COPD treatment. PMID: 33459100 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 21, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Impact of Interferon- γ (IFN-γ) and IFN-γ-Inducible Protein 10 (IP-10) Genes' Polymorphism on Risk of Hepatitis C Virus-Related Liver Cirrhosis.
Conclusion: Our data support the concept that IFN-γ and IP-10 gene polymorphisms are not predictors of disease progression among Egyptian patients with HCV infection. PMID: 33445993 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 17, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Evaluation of IL1B rs1143634 and IL6 rs1800796 Polymorphisms with Autism Spectrum Disorder in the Turkish Children.
Conclusion: This study concludes that rs1143634 is associated with the risk of ASD in Turkish children. Determining these polymorphisms in a larger sample group may contribute to understanding the etiology of ASD and developing new treatment protocols. Abbreviations: ASD: Autism spectrum disorder; DNA: Deoxyribonucleic acid; IL6: Interleukin 6; IL1B: Interleukin 1 beta; Real-time PCR: Real-time polymerase chain reaction; JAK-STAT: The Janus kinase/signal transducers and activators of transcription; MAPK: The mitogen-activated protein kinase; 5'UTR: The 5' untranslated region; IL1α: Interleukin 1 alpha; IL-1Ra: Interl...
Source: Immunological Investigations - January 14, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Prevalence of Selective and Partial Immunoglobulin A Deficiency in Patients with Autoimmune Polyendocrinopathy.
Authors: Jamee M, Alaei MR, Mesdaghi M, Noorian S, Moosavian M, Dolatshahi E, Taghavi Kojidi H, Chavoshzadeh Z, Fallahi M, Parviz S, Aghamahdi F, Azizi G Abstract Background: Autoimmune disorders are reported as presenting signs in patients with immunoglobulin A (IgA) deficiency. Herein, we aim to evaluate serum IgA among patients with autoimmune polyendocrinopathy. Methods: Patients with two or more autoimmune endocrinopathies were selected and the serum IgA levels were measured. Patients with an isolated low serum IgA (
Source: Immunological Investigations - January 14, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Antioxidant and Immunomodulatory Properties of Partially purified Exopolysaccharide from Lactobacillus Casei Isolated from Chinese Northeast Sauerkraut.
Conclusion: Our results showed the protective effect against oxidative damage and potential immunomodulatory and anti-inflammatory properties of EPS from Lactobacillus casei NA-2. PMID: 33416001 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 10, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

In Silico and in Vivo Analysis of HIV-1 Rev Regulatory Protein for Evaluation of a Multiepitope-based Vaccine Candidate.
Authors: Shabani SH, Kardani K, Milani A, Bolhassani A Abstract In silico-designed multiepitope conserved regions of human immunodeficiency virus 1 (HIV-1) proteins would be a beneficial strategy for antigen design which induces effective anti-HIV-1 T-cell responses. The conserved multiple HLA-DR-binding epitopes of Rev protein were identified using IEDB MHC-I prediction tools and SYFPEITHI webserver to screen potential T-cell epitopes. We analyzed toxicity, allergenicity, immunogenicity, hemolytic activity, cross-reactivity, cell-penetrating peptide (CPP) potency, and molecular docking of the candidate epitop...
Source: Immunological Investigations - January 10, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Evaluation of Serum Immunoreactivity to Common Indigenous Iranian Inhalation and Food Allergens in Patients with Meniere's Disease.
Conclusion: Serum total IgE level in patients with MD (in both inhalation and food allergens groups) was higher than the control group, and there was a relationship between MD and immunoreactivity to common indigenous inhalation and food allergens of Iran. PMID: 33416011 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 10, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The First Iranian Cohort of Pediatric Patients with Activated Phosphoinositide 3-Kinase- δ (PI3Kδ) Syndrome (APDS).
Conclusion: Respiratory tract infections and humoral immunodeficiency are commonly the most important complication in pediatric APDS patients, and they can be fatal by ultimately causing catastrophic damage to the structure of lungs. Hence, physicians should be aware of its significance and further work-up of patients with recurrent respiratory tract infections especially in patients with lymphoproliferation. Moreover, delineation of genotype-phenotype associations with disease severity could be helpful in the timely application of appropriate management and patients' survival. PMID: 33401995 [PubMed - as supplied by p...
Source: Immunological Investigations - January 8, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Expression and Clinical Significance of KLRG1 and 2B4 on T Cells in the Peripheral Blood and Tumour of Patients with Cervical Cancer.
Authors: Guo Y, Feng Y, Fan P, Yao X, Peng Y, Wang R, Kuerban G Abstract Background: Killer cell lectin-like receptor G1 (KLRG1) and 2B4 play important roles in the immune regulation and immune tolerance to tumor cells by inhibiting T cell function. However, the clinical relevance of KLRG1 and 2B4 to cervical cancer remains to be understood. Methods: We measured the frequency of KLRG1+ or 2B4+ cells in CD4+ or CD8 + T cells derived from peripheral blood or tumour biopsies in cervical cancer patients by flow cytometry. Results: Compared with healthy controls, the level of KLRG1 and 2B4 on CD8&nbs...
Source: Immunological Investigations - January 8, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Lack of Associations between Endoplasmic Reticulum Aminopeptidase 2 Gene Polymorphisms and Ankylosing Spondylitis: A Meta-analysis with Trial Sequential Analysis.
Conclusion: The present findings of this study do not support any evidence on the associations of rs2248374 and rs2549782 polymorphisms in the ERAP2 gene and susceptibility to AS. Additional well-designed and large-sample studies in diverse ethnicities are encouraged to validate the current findings. PMID: 33401999 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 8, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Statement of Retraction: The Association of Interleukin-16 Gene Polymorphisms with IL-16 Serum Levels and Risk of Multiple Sclerosis.
Authors: Farrokhi M, Masoudifar A, Derakhshan A, Saadatmand S, Rouhi-Boroujeni H, Etemadifar M, Rezaei-Zarji S, Javid A, Nobakht R, Deyhimi M, Ekramnia A, Ebrahimi M, Sheikh S, Ansaripour S, Amani-Beni A, Jahanbani-Ardakani H PMID: 33402009 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - January 8, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Polymorphisms of Interleukin-12B Gene and Susceptibility to Inflammatory Bowel Diseases: A Meta-analysis and Trial Sequential Analysis.
Conclusion: IL-12B rs6887695 polymorphism was associated with increased risk of CD and UC, while IL-12B rs10045431 polymorphism might only be correlated with the risk of UC. Abbreviations: IBD: inflammatory bowel disease; CD: Crohn's disease; UC: ulcerative colitis; IL-12B: interleukin-12B; OR: odds ratio; CI: confidence interval; TSA: trial sequential analysis; RIS: required information size; DCs: dendritic cells; NK: nature killer; APCs: antigen-presenting cells; TNF: tumor necrosis factor; SNP: single nucleotide polymorphisms; HWE: Hardy-Weinberg equilibrium; NOS: Newcastle-Ottawa scale; RRR: relative risk reduction. ...
Source: Immunological Investigations - January 5, 2021 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

A Differential Immune Modulating Role of Vitamin D in Urinary Tract Infection.
Authors: Pirdel L, Pirdel M Abstract Vitamin D is known as an important modulator of numerous immune functions. We aimed to investigate the association of 25-hydroxyvitamin D [25(OH)D] with several humoral mediators of the immune system in the patients with urinary tract infection (UTI) caused by uropathogenic E. coli (UPEC). Serum levels of 25(OH)D, cytokines (interferon (IFN)-γ, interleukin (IL)-4, IL-6, IL-10, IL-17A, tumor necrosis factor transforming growth factor (TNF)-α, and tumor growth factor (TGF)-β), immunoglobulin (Ig) isotypes (IgG, IgM, and IgM), complement proteins (C3 and C4) w...
Source: Immunological Investigations - December 29, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of Toll-like Receptor 4 Rs7873784 G/C Polymorphism with Rheumatoid Arthritis Risk in a Chinese Population.
We examined the TLR4 rs7873784 G/C polymorphism in 805 Chinese RA patients and 1095 healthy controls. Genotype was determined with a custom-by-design 48-Plex single nucleotide polymorphism scan™ Kit. Blood plasma levels of TLR4 in 170 RA patients and 170 matched controls were measured by ELISA. Results: The TLR4 gene rs7873784 G/C polymorphism was related to a reduced risk for RA. By stratified analysis, we found a dramatically reduced risk for RA in patients who were female, CRP-positive, RF-positive, DAS28 ≥ 3.20, or ESR ≥ 25. Compared with the controls, the average level of TLR4 protein in plasma o...
Source: Immunological Investigations - December 29, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Nephroprotection through Modifying the Apoptotic TNF- α/ERK1/2/Bax Signaling Pathway and Oxidative Stress by Long-term Sodium Hydrosulfide Administration in Ovalbumin-induced Chronic Asthma.
Nephroprotection through Modifying the Apoptotic TNF-α/ERK1/2/Bax Signaling Pathway and Oxidative Stress by Long-term Sodium Hydrosulfide Administration in Ovalbumin-induced Chronic Asthma. Immunol Invest. 2020 Dec 21;:1-17 Authors: Kianian F, Seifi B, Kadkhodaee M, Sadeghipour HR, Ranjbaran M Abstract Asthma is one of the most common respiratory diseases in the world. Nevertheless, it is reported that inflammation induced by asthma is not only restricted to the lung and may cause damaging effects on remote organs. Therefore, this study was designed to investigate the beneficial effects of long-t...
Source: Immunological Investigations - December 22, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Investigation of Serum Total IgE and Eosinophil Levels in Different Allergic Diseases Together with the Study of Their Correlations with Various Possible Allergens in Mosul City.
Authors: Altaii HA, Al-Tae FMD Abstract Serum total IgE (tIgE) is frequently used in the diagnosis of allergic diseases in Mosul city and Iraq. However, neither normal nor elevated levels can exclude or confirm the diagnosis of these diseases. Here, we tried to evaluate the validity of tIgE in the diagnosis of allergic asthma(AS), allergic rhinitis(AR) and atopic dermatitis(AD) in a sample of Mosul population, to set up an optimum cut-off value for diagnosis of these diseases and to study its correlation with different parameters that might affect its level. 38 patients with AS, 27 with AR, 46 with AD and 45 health...
Source: Immunological Investigations - December 9, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Development of a Novel Multiplex Bead-based Assay for Measuring Autoantibodies on Flow Cytometric Platform.
Authors: Chauhan R, Gupta N, Tiwari AK, Raina V, Nandi SP Abstract Background: Autoantibodies (AAbs) are important biomarkers for the diagnosis of Autoimmune Diseases (ADs). The detection of AAbs performed by current methods (indirect immunofluorescence test (IIFT)/Immunoblot (dot/line)/enzyme-linked immunosorbent assay ELISA) which have limitations in terms of performing multiple assays to arrive at laboratory diagnosis. We validated a novel multiplex bead-based assay (NMBA) that could quantify five common antibodies, simultaneously, on a flow-cytometry platform. Methods: A total of five recombinant antigens (SS-A...
Source: Immunological Investigations - December 9, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of TNF- α G-308 a Promoter Polymorphism with the Course and Outcome of COVID-19 Patients.
Conclusion: People who carry the A allele of TNF-ɑ polymorphism are more prone to COVID-19 infection. The AA genotype of TNF-ɑ is associated with a more aggressive pattern of the disease. In those patients, the use of anti - TNF therapy may be promising. PMID: 33228423 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - November 25, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

MiR-135b Alleviates Airway Inflammation in Asthmatic Children and Experimental Mice with Asthma via Regulating CXCL12.
CONCLUSION: MiR-135b may inhibit immune responses of Th17 cells to alleviate airway inflammation and hyperresponsiveness in asthma possibly by targeting CXCL12, showing the potential value in asthma treatment. PMID: 33203292 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - November 21, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Evaluation of Expression of LRBA and CTLA-4 Proteins in Common Variable Immunodeficiency Patients.
In this study, 20 newly diagnosed CVID patients without infection only phenotype, and ten healthy individuals were enrolled. The expressions of LRBA and CTLA-4 proteins were assessed by western blotting and flow cytometry, respectively. The patients were divided into two groups of autoimmunity-positive (11 cases) and autoimmunity-negative (9 patients). LRBA and CTLA-4 expressions were significantly lower in autoimmune-positive patients than in healthy individuals (P = .03 and P = .03, respectively). Autoimmune-negative patients had lower expression of LRBA and CTLA-4 than the control group, although it was not si...
Source: Immunological Investigations - November 18, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of TNFSF4 Gene Polymorphisms and Plasma TNFSF4 Level with Risk of Systemic Lupus Erythematosus in a Chinese Population.
Authors: Yue LY, Xu Y, Tao B, He CS Abstract Background: Systemic lupus erythematosus (SLE) is an autoimmune disease. Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is an inflammatory factor that has been discussed in different inflammatory diseases and cancers. However, relationship between TNFSF4 and SLE is limited. Material and methods: The present case-control study recruited 400 SLE patients and 600 healthy controls from Southern Chinese Han origin. Plasma levels of TNFSF4 were tested by enzyme linked-immunosorbent assay, and association of rs2205960, rs704840, rs844648, rs3850641 and rs17568 polym...
Source: Immunological Investigations - November 11, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Higher Peripheral Blood IFN- γ-/IL-4+ Th2 Lymphocytes Are Associated with Lower Rate of Relapse in Patients with Lymphoma.
CONCLUSION: Taken together, higher peripheral blood IFN-γ-/IL-4+ Th2 lymphocytes might be associated with a favorable prognosis like lower rate of relapse in lymphoma patients. PMID: 33148063 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - November 6, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Tetrandrine Attenuates Cartilage Degeneration, Osteoclast Proliferation, and Macrophage Transformation through Inhibiting P65 Phosphorylation in Ovariectomy-induced Osteoporosis.
CONCLUSION: TET attenuated bone structural parameters including BMD, BV/TV, Tb.N, Tb.Th and Tb.Sp, inhibited articular cartilage degeneration, promoted bone formation, inhibited the inflammatory response, and promoted macrophage transformation from M1 to M2 via NF-κB inactivation in osteoporosis. TET may be a promising drug for osteoporosis therapy. ABBREVIATION: TET: Tetrandrine; ACLT: anterior cruciate ligament transection; OVX: ovariectomy; TRAP: Tartrate-resistant acid phosphatase; BMD: bone mineral density; BV/TV: Bone volume/total volume; Tb.Th: trabecular thickness; Tb.N: trabecular number; Tb.Sp: trabecul...
Source: Immunological Investigations - November 5, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association of Polymorphism in IL-18 Gene with Periodontitis in Uyghur Adults in Xinjiang and Evidence from Six Case-Control Studies with a Comprehensive Analysis.
CONCLUSIONS: IL-18 137 G > C, 607 C > A could be associated with susceptibility to periodontitis in Uyghur population. Further case-control of candidate genes studies targeting larger sample sizes and different ethnic groups are needed to arrive more accurate conclusions. PMID: 33143466 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - November 5, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Assessment of the Relationship between Clinical Variants of Psoriasis and Killer Immunoglobulin-like Receptor (KIR) Genes: A Systematic Review with Meta-analysis.
CONCLUSION: This meta-analysis demonstrates that subjects that carry the KIR2DS1 gene could have a potential risk factor for the development of PsV. Conversely, KIR2DS4 and 3DL1 genes appear to confer protection against PsV. PMID: 33115277 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 30, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Increased Peripheral NKG2A-NKG2D+CD3-CD16+CD56dim NK Cell Subset Was Positively Correlated with Antiphospholipid Antibodies in Patients of Obstetric Antiphospholipid Syndrome.
In this study, we compared the distributional profiles of different NK cell subsets and the expressions of NK cell-activating receptors in peripheral blood of patients with OAPS and healthy women. Our results showed significantly increased NKG2A-NKG2D+ subset and decreased NKG2A+NKG2D- subset in CD3- CD16+CD56dim NK cells, CD3-CD16-CD56bright NK cells and CD56+T cells in OAPS patients compared with those in healthy control women. The CD27-CD11b+ subset significantly increased in CD3-CD16+CD56dim NK cells in OAPS patients compared with those in healthy control women. In addition, the NKG2A-NKG2D+ subset in CD3-CD16 + CD56di...
Source: Immunological Investigations - October 27, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Relationship between PTPN22 R620W Polymorphisms and the Susceptibility to Autoimmune Thyroid Diseases: An Updated Meta-analysis.
Authors: Wu H, Wan S, Qu M, Ren B, Liu L, Shen H Abstract The protein tyrosine phosphatase non-receptor 22 (PTPN22) R620W polymorphism has been related to susceptibility to autoimmune thyroid disease (AITD) with inconsistent results. Therefore, this meta-analysis was designed to assess a more accurate association between the PTPN22 R620W polymorphism and AITD susceptibility. A systematic search of the EMBASE, PubMed, Web of Science, CBM, CNKI, and WanFang databases was performed to determine relevant publications. Statistical analyses of the odds ratios (ORs), 95% confidence intervals (CIs), and p values were perfo...
Source: Immunological Investigations - October 27, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Tumor Necrosis Factor-alpha (TNF- α) -238 G/A Polymorphism Is Associated with the Treatment Resistance and Attempted Suicide in Schizophrenia.
Tumor Necrosis Factor-alpha (TNF-α) -238 G/A Polymorphism Is Associated with the Treatment Resistance and Attempted Suicide in Schizophrenia. Immunol Invest. 2020 Oct 22;:1-13 Authors: Aytac HM, Ozdilli K, Tuncel FC, Pehlivan M, Pehlivan S Abstract Abnormality of the immune system may play an important role in the pathogenesis of schizophrenia (SCZ). We aim to investigate the relationship between clinical features of SCZ and tumor necrosis factor-alpha (TNF-α) -238 G/A, -308 G/A polymorphisms in SCZ patients by comparing genotype distributions of TNF-α gene polymorphisms bet...
Source: Immunological Investigations - October 25, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Identification of Linear Peptide Immunogens with Verified Broad-spectrum Immunogenicity from the Conserved Regions within the Hemagglutinin Stem Domain of H1N1 Influenza Virus.
Conclusion: The linear epitopes fused with ferritin identified in this study can lay the foundation for future advancements in development of broad-spectrum subunit vaccine against IAV (H1N1), and give rise to the potential future applicability of ferritin-based antigen delivery nanoplatforms. PMID: 33078652 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 22, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Relationship between Extracellular/intracellular microRNAs and TLRs May Be Used as a Diagnostic and Therapeutic Approach in Sepsis.
In conclusion, this study has provided new insight into the role of microRNAs as a regulator of the TLRs which may lead to the aberrant inflammatory response in sepsis. Therefore, it suggests that both intracellular and extracellular microRNAs may play a therapeutic role in the treatment of sepsis via regulating TLRs. However, yet sepsis and septic shock are medical emergencies and further studies are needed to specify the exact role of microRNAs and TLRs in sepsis. PMID: 33054447 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 20, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

The Responsiveness of Thymic Stromal Cells to semaphorin-3A.
CONCLUSION: Our data strongly suggest the active participation of Sema3A in thymic physiology, highlighting its role as an immunomodulatory molecule. This may provide important knowledge for understanding the interactions of thymic cells. PMID: 33070651 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 20, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Letter from the Editor 2020: Annual Summary and Introduction of the Thematic Issue and Guest Editor.
Authors: Rittenhouse-Olson K PMID: 33043771 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - October 15, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

When Little Things Make a Big Difference.
Authors: Shenoy GN PMID: 33043772 [PubMed - in process] (Source: Immunological Investigations)
Source: Immunological Investigations - October 15, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research

Association between TH2 Cytokine Gene Polymorphisms and Risk of Bullous Pemphigoid.
Conclusions: Our results indicate that IL-13 rs1800925 variation may be a protective genetic marker for the development of BP. Given this preventive effect against BP, therapeutic strategies could potentially be developed interfering with the functions of IL-13 cytokine, which seems to be integral in the pathogenesis of eosinophilic inflammatory disorders, such as BP. PMID: 33047635 [PubMed - as supplied by publisher] (Source: Immunological Investigations)
Source: Immunological Investigations - October 15, 2020 Category: Allergy & Immunology Tags: Immunol Invest Source Type: research