Fever as an evolutionary agent to select immune complexes interfaces
AbstractWe herein analyzed all available protein –protein interfaces of the immune complexes from the Protein Data Bank whose antigens belong to pathogens or cancers that are modulated by fever in mammalian hosts. We also included, for comparison, protein interfaces from immune complexes that are not significantly modulated by the fever response . We highlight the distribution of amino acids at these viral, bacterial, protozoan and cancer epitopes, and at their corresponding paratopes that belong strictly to monoclonal antibodies. We identify the “hotspots”, i.e. residues that are highly connected at such interfaces,...
Source: Immunogenetics - May 11, 2022 Category: Genetics & Stem Cells Source Type: research
TLR7 and TLR8 evolution in lagomorphs: different patterns in the different lineages
AbstractToll-like receptors (TLRs) are one of the most ancient and widely studied innate immune receptors responsible for host defense against invading pathogens. Among the known TLRs, TLR7 and TLR8 sense and recognize single-stranded (ss) RNAs with a dynamic evolutionary history. While TLR8 was lost in birds and duplicated in turtles and crocodiles, TLR7 is duplicated in some birds, but in other tetrapods, there is only one copy. In mammals, with the exception of lagomorphs, TLR7 and TLR8 are highly conserved. Here, we aim to study the evolution of TLR7 and TLR8 in mammals, with a special focus in the order Lagomorpha. By...
Source: Immunogenetics - April 14, 2022 Category: Genetics & Stem Cells Source Type: research
COVID-19: impact on Public Health and hypothesis-driven investigations on genetic susceptibility and severity
AbstractCOVID-19 is a new complex multisystem disease caused by the novel coronavirus SARS-CoV-2. In slightly over 2 years, it infected nearly 500 million and killed 6 million human beings worldwide, causing an unprecedented coronavirus pandemic. Currently, the international scientific community is engaged in elucidating the molecular mechanisms of the pathophysiology of SARS-CoV-2 infection as a basis of scient ific developments for the future control of COVID-19. Global exome and genome analysis efforts work to define the human genetics of protective immunity to SARS-CoV-2 infection. Here, we review the current knowled...
Source: Immunogenetics - March 29, 2022 Category: Genetics & Stem Cells Source Type: research
Myd88 knockdown with RNA interference induces in vitro immune hyporesponsiveness in dendritic cells from rhesus monkeys
In conclusion, our data indicated that Myd88 silencing could inhibit the maturation of imDCs and alleviate immune rejection, which provides a reference for immune tolerance in clinical liver transplantation. (Source: Immunogenetics)
Source: Immunogenetics - March 18, 2022 Category: Genetics & Stem Cells Source Type: research
Identification of G-quadruplex DNA sequences in SARS-CoV2
AbstractG-quadruplex structure or Putative Quadruplex Sequences (PQSs) are abundant in human, microbial, DNA, or RNA viral genomes. These sequences in RNA viral genome play critical roles in integration into human genome as LTR (Long Terminal Repeat), genome replication, chromatin rearrangements, gene regulation, antigen variation (Av), and virulence. Here, we investigated whether the genome of SARS-CoV2, an RNA virus, contained such potential G-quadruplex structures. Using bioinformatic tools, we searched for such sequences and found thirty-seven (forward strand (twenty-five) + reverse strand (Twelve)) QGRSs (Quadrup...
Source: Immunogenetics - March 18, 2022 Category: Genetics & Stem Cells Source Type: research
Myd88 knockdown with RNA interference induces in vitro immune hyporesponsiveness in dendritic cells from rhesus monkeys
In conclusion, our data indicated that Myd88 silencing could inhibit the maturation of imDCs and alleviate immune rejection, which provides a reference for immune tolerance in clinical liver transplantation. (Source: Immunogenetics)
Source: Immunogenetics - March 18, 2022 Category: Genetics & Stem Cells Source Type: research
Identification of G-quadruplex DNA sequences in SARS-CoV2
AbstractG-quadruplex structure or Putative Quadruplex Sequences (PQSs) are abundant in human, microbial, DNA, or RNA viral genomes. These sequences in RNA viral genome play critical roles in integration into human genome as LTR (Long Terminal Repeat), genome replication, chromatin rearrangements, gene regulation, antigen variation (Av), and virulence. Here, we investigated whether the genome of SARS-CoV2, an RNA virus, contained such potential G-quadruplex structures. Using bioinformatic tools, we searched for such sequences and found thirty-seven (forward strand (twenty-five) + reverse strand (Twelve)) QGRSs (Quadrup...
Source: Immunogenetics - March 18, 2022 Category: Genetics & Stem Cells Source Type: research
Comparative genetics of KIR haplotype diversity in humans and rhesus macaques: the balancing act
This study exhibits highly diverse haplotype architectures in humans and macaques, which nevertheless might have an equivalent effect on the modulation of NK cell activity. (Source: Immunogenetics)
Source: Immunogenetics - March 15, 2022 Category: Genetics & Stem Cells Source Type: research
iTTCA-MFF: identifying tumor T cell antigens based on multiple feature fusion
AbstractCancer is a terrible disease, recent studies reported that tumor T cell antigens (TTCAs) may play a promising role in cancer treatment. Since experimental methods are still expensive and time-consuming, it is highly desirable to develop automatic computational methods to identify tumor T cell antigens from the huge amount of natural and synthetic peptides. Hence, in this study, a novel computational model called iTTCA-MFF was proposed to identify TTCAs. In order to describe the sequence effectively, the physicochemical (PC) properties of amino acid and residue pairwise energy content matrix (RECM) wer e firstly e...
Source: Immunogenetics - March 5, 2022 Category: Genetics & Stem Cells Source Type: research
