Detection of retinal dysfunction induced by HCN channel inhibitors using multistep light stimulus and long-duration light stimulus ERG in rats
This study revealed that the retinal dysfunction by HCN channel inhibitors in rats can be detected by multistep light stimulus ERG. Additionally, we identified that the inhibition of feedback current and the sustained responses in the photoreceptor cells cause the retinal dysfunction of HCN channel inhibitors in rats.PMID:38401854 | DOI:10.1016/j.exer.2024.109847 (Source: Experimental Eye Research)
Source: Experimental Eye Research - February 24, 2024 Category: Opthalmology Authors: Naohisa Umeya Yuki Yoshizawa Kosuke Fukuda Keigo Ikeda Mami Kamada Hiroshi Inada Toru Usui Izuru Miyawaki Source Type: research
Extracellular vesicle biomarkers in ocular fluids associated with ophthalmic diseases
Exp Eye Res. 2024 Feb 22:109831. doi: 10.1016/j.exer.2024.109831. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are released as highly stable lipid bilayer particles carrying proteins, lipids, glycans and miRNAs. The contents of EVs vary based on the cellular origin, biogenesis route and the functional state of the cell suggesting certain diseased conditions. A growing body of evidence show that EVs carry important molecules implicated in the development and progression of ophthalmic diseases. EVs associated with ophthalmic diseases are mainly carried by one of the three ocular biofluids which include tears, a...
Source: Experimental Eye Research - February 24, 2024 Category: Opthalmology Authors: Thomas Michael Shiju Alex Yuan Source Type: research
Isolation and characterization of rabbit limbal niche cells
In conclusion, rabbit-LNCs could be successfully isolated by the collagenase A digestion method as used in human tissue. There were similar characteristics between rabbit and human LNCs (VIM+/CD90+/CD105+/SCF+/PAX6+/PDGFRβ+).PMID:38395213 | DOI:10.1016/j.exer.2024.109838 (Source: Experimental Eye Research)
Source: Experimental Eye Research - February 23, 2024 Category: Opthalmology Authors: Guanyu Su Xiaojie Guo Lingjuan Xu Bihui Jin Yongyao Tan Xiao Zhou Wei Wang Xinyu Li Shusheng Wang Guigang Li Source Type: research
TGF- β2-induced alteration of m6A methylation in hTERT RPE-1 cells
Exp Eye Res. 2024 Feb 21:109839. doi: 10.1016/j.exer.2024.109839. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) is a major type of RNA modification implicated in various pathophysiological processes. Transforming growth factor β2 (TGF-β2) induces epithelial-mesenchymal transition (EMT) in retinal pigmental epithelial (RPE) cells and promotes the progression of proliferative vitreoretinopathy (PVR). However, the role of m6A methylation in the EMT of human telomerase reverse transcriptase (hTERT) retinal pigmental epithelium (RPE)-1 cells has not been clarified. Here, we extracted RNA from RPE cells subjected to 0...
Source: Experimental Eye Research - February 23, 2024 Category: Opthalmology Authors: Xue Li Xueru Zhao Ruijie Yin Min Yuan Yongya Zhang Xiaohua Li Source Type: research
Therapeutic effect and mechanism of action of pterostilbene nano drugs in dry eye models
Exp Eye Res. 2024 Feb 20:109836. doi: 10.1016/j.exer.2024.109836. Online ahead of print.ABSTRACTDry eye disease is a multifactorial dysfunction of the tear film and ocular surface, with etiology involving inflammation and oxidative stress on the ocular surface. Pterostilbene (PS) is a secondary metabolite extracted from plants, which possesses remarkable anti-inflammatory and antioxidant effects. However, its application is limited by light instability and very poor water solubility. We modified fat-soluble PS into a biparental pterostilbene-glutaric anhydride-arginine-glycine-aspartic acid (PS-GA-RGD) nanomedicine by prod...
Source: Experimental Eye Research - February 22, 2024 Category: Opthalmology Authors: Kexin Li Meng Lin Kaiyan Huang Jiaxin Han Linzhi Wei Lijie Miao Huijuan Chen Qianwen Gong Xingyi Li Liang Hu Source Type: research
Therapeutic effect and mechanism of action of pterostilbene nano drugs in dry eye models
Exp Eye Res. 2024 Feb 20:109836. doi: 10.1016/j.exer.2024.109836. Online ahead of print.ABSTRACTDry eye disease is a multifactorial dysfunction of the tear film and ocular surface, with etiology involving inflammation and oxidative stress on the ocular surface. Pterostilbene (PS) is a secondary metabolite extracted from plants, which possesses remarkable anti-inflammatory and antioxidant effects. However, its application is limited by light instability and very poor water solubility. We modified fat-soluble PS into a biparental pterostilbene-glutaric anhydride-arginine-glycine-aspartic acid (PS-GA-RGD) nanomedicine by prod...
Source: Experimental Eye Research - February 22, 2024 Category: Opthalmology Authors: Kexin Li Meng Lin Kaiyan Huang Jiaxin Han Linzhi Wei Lijie Miao Huijuan Chen Qianwen Gong Xingyi Li Liang Hu Source Type: research
The response of retinal ganglion cells to optical defocused visual stimuli in mouse retinas
This study aimed to investigate the response characteristics of Retinal Ganglion Cells (RGCs), represented by alpha (α) RGCs, when exposed to focused, simulated defocused pattern images and astigmatically defocused images projected onto mouse retinas. Negative pressure was applied to stretch the soma of RGC in vitro to simulate myopia using a 7-8 μm diameter glass microelectrode, resulting in a 5% increase in the cell's diameter. A custom-made device was utilized to project spherically (equal to ±10 and ± 20 D) and astigmatically (+6.00D) defocused images onto the retinas. As a control for a deficient intact retinal ci...
Source: Experimental Eye Research - February 21, 2024 Category: Opthalmology Authors: Chunghim So Ting Zhang Qin Wang ChunTing Qiu Lestrange-Anginieur Elie de Feng Pan Source Type: research
Human lens epithelial-secreted exosomes attenuate ocular angiogenesis via inhibiting microglial activation
Exp Eye Res. 2024 Feb 19:109837. doi: 10.1016/j.exer.2024.109837. Online ahead of print.ABSTRACTThe lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced choroidal neovascularization (laser-induced CNV). Furthermore, the activation and accumulation of microglia were also rest...
Source: Experimental Eye Research - February 21, 2024 Category: Opthalmology Authors: Yan Wu Jiagui Wang Ting Pan Jie Lei Yuanyuan Fan Jingfan Wang Changlin Xu Qinyuan Gu Xingxing Wang Tianhao Xiao Qinghuai Liu Ping Xie Zizhong Hu Source Type: research
The response of retinal ganglion cells to optical defocused visual stimuli in mouse retinas
This study aimed to investigate the response characteristics of Retinal Ganglion Cells (RGCs), represented by alpha (α) RGCs, when exposed to focused, simulated defocused pattern images and astigmatically defocused images projected onto mouse retinas. Negative pressure was applied to stretch the soma of RGC in vitro to simulate myopia using a 7-8 μm diameter glass microelectrode, resulting in a 5% increase in the cell's diameter. A custom-made device was utilized to project spherically (equal to ±10 and ± 20 D) and astigmatically (+6.00D) defocused images onto the retinas. As a control for a deficient intact retinal ci...
Source: Experimental Eye Research - February 21, 2024 Category: Opthalmology Authors: Chunghim So Ting Zhang Qin Wang ChunTing Qiu Lestrange-Anginieur Elie de Feng Pan Source Type: research
Human lens epithelial-secreted exosomes attenuate ocular angiogenesis via inhibiting microglial activation
Exp Eye Res. 2024 Feb 19:109837. doi: 10.1016/j.exer.2024.109837. Online ahead of print.ABSTRACTThe lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced choroidal neovascularization (laser-induced CNV). Furthermore, the activation and accumulation of microglia were also rest...
Source: Experimental Eye Research - February 21, 2024 Category: Opthalmology Authors: Yan Wu Jiagui Wang Ting Pan Jie Lei Yuanyuan Fan Jingfan Wang Changlin Xu Qinyuan Gu Xingxing Wang Tianhao Xiao Qinghuai Liu Ping Xie Zizhong Hu Source Type: research
Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice
Exp Eye Res. 2024 Feb 17:109835. doi: 10.1016/j.exer.2024.109835. Online ahead of print.ABSTRACTThe etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of CACNA2D1 (encoding α2δ1), a calcium voltage-gated channel auxiliary subunit, has been reported to be associated with primary open-angle glaucoma, and the pharmacological mitigation of α2δ1 activity by pregabalin lowers IOP, the cellular basis for α2δ1 role in the modulation of IOP remains unclear. Our recent findings reveled readily detecta...
Source: Experimental Eye Research - February 19, 2024 Category: Opthalmology Authors: Levi Lankford Rupalatha Maddala Monica M Jablonski P Vasantha Rao Source Type: research
Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice
Exp Eye Res. 2024 Feb 17:109835. doi: 10.1016/j.exer.2024.109835. Online ahead of print.ABSTRACTThe etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of CACNA2D1 (encoding α2δ1), a calcium voltage-gated channel auxiliary subunit, has been reported to be associated with primary open-angle glaucoma, and the pharmacological mitigation of α2δ1 activity by pregabalin lowers IOP, the cellular basis for α2δ1 role in the modulation of IOP remains unclear. Our recent findings reveled readily detecta...
Source: Experimental Eye Research - February 19, 2024 Category: Opthalmology Authors: Levi Lankford Rupalatha Maddala Monica M Jablonski P Vasantha Rao Source Type: research
Allele-specific antisense oligonucleotides for the treatment of BEST1-Related dominantly inherited retinal diseases: An in vitro model
Exp Eye Res. 2024 Feb 16:109833. doi: 10.1016/j.exer.2024.109833. Online ahead of print.ABSTRACTRetinal dystrophies are a common health problem worldwide that are currently incurable due to the inability of retinal cells to regenerate. Inherited retinal diseases (IRDs) are a diverse group of disorders characterized by progressive vision loss caused by photoreceptor cell dysfunction. The eye has always been an attractive organ for the development of novel therapies due to its independent access to the systemic pathway. Moreover, anti-sense oligonucleotides (ASOs), which facilitate manipulation of unwanted mRNAs via degradat...
Source: Experimental Eye Research - February 18, 2024 Category: Opthalmology Authors: Beren Karaosmanoglu Gozde Imren Eda Utine Hande Taylan Sekeroglu Ekim Z Taskiran Source Type: research
Long noncoding RNA ZFAS1: A novel anti-apoptotic target in Fuchs endothelial corneal dystrophy
This study would broaden our understanding of epigenetic regulation in FECD development, and provide potential anti-apoptotic targets for FECD therapy.PMID:38369232 | DOI:10.1016/j.exer.2024.109832 (Source: Experimental Eye Research)
Source: Experimental Eye Research - February 18, 2024 Category: Opthalmology Authors: Jini Qiu Ruiping Gu Qian Shi Xueling Zhang Jiayu Gu Jun Xiang Jianjiang Xu Yujing Yang Kun Shan Source Type: research
Allele-specific antisense oligonucleotides for the treatment of BEST1-Related dominantly inherited retinal diseases: An in vitro model
Exp Eye Res. 2024 Feb 16:109833. doi: 10.1016/j.exer.2024.109833. Online ahead of print.ABSTRACTRetinal dystrophies are a common health problem worldwide that are currently incurable due to the inability of retinal cells to regenerate. Inherited retinal diseases (IRDs) are a diverse group of disorders characterized by progressive vision loss caused by photoreceptor cell dysfunction. The eye has always been an attractive organ for the development of novel therapies due to its independent access to the systemic pathway. Moreover, anti-sense oligonucleotides (ASOs), which facilitate manipulation of unwanted mRNAs via degradat...
Source: Experimental Eye Research - February 18, 2024 Category: Opthalmology Authors: Beren Karaosmanoglu Gozde Imren Eda Utine Hande Taylan Sekeroglu Ekim Z Taskiran Source Type: research
