Reduction of lens size in PAX6-related aniridia
Exp Eye Res. 2023 Dec 5;238:109746. doi: 10.1016/j.exer.2023.109746. Online ahead of print.ABSTRACTHeterozygous mutation of PAX6 in humans leads to congenital aniridia (OMIM 106210) which is typified by congenital iris and foveal defects, and later onset glaucoma, aniridic keratopathy, and cataract. Mice heterozygous for Pax6 mutations phenocopy many aspects of aniridia including the iris defects, keratopathy and cataract, although Pax6 mutant mice have small lenses, a phenotype which is not typically reported in human aniridia, perhaps due to difficulties in measuring lens diameter during typical ophthalmic examinations a...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Melinda K Duncan Alejandra Daruich Sophie Valleix Dominique Bremond-Gignac Source Type: research
Visualization of choroidal vasculature in pigmented mouse eyes from experimental models of AMD
Exp Eye Res. 2023 Dec 7;238:109741. doi: 10.1016/j.exer.2023.109741. Online ahead of print.ABSTRACTA variety of techniques exist to investigate retinal and choroidal vascular changes in experimental mouse models of human ocular diseases. While all have specific advantages, a method for evaluating the choroidal vasculature in pigmented mouse eyes has been more challenging especially for whole mount visualization and morphometric analysis. Here we report a simple, reliable technique involving bleaching pigment prior to immunostaining the vasculature in whole mounts of pigmented mouse choroids. Eyes from healthy adult pigment...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Imran A Bhutto D Scott McLeod Benjamin R Thomson Gerard A Lutty Malia M Edwards Source Type: research
Trolox aids coenzyme Q < sub > 10 < /sub > in neuroprotection against NMDA induced damage via upregulation of VEGF in rat model of glutamate excitotoxicity
Exp Eye Res. 2023 Dec 4;238:109740. doi: 10.1016/j.exer.2023.109740. Online ahead of print.ABSTRACTGlutamate induced damage to retinal ganglion cells (RGCs) requires tight physiological regulation of the N-methyl-D-aspartate (NMDA) receptors. Previously, studies have demonstrated the neuroprotective abilities of antioxidants like coenzyme Q10 (CoQ10) and vitamin E analogs like α-tocopherol against neuropathies resulting from NMDA insult, but have failed to shed light on the effect of CoQ10 and trolox, a hydrophilic analog of vitamin E, on glaucomatous neurodegeneration. In the current study, we wanted to investigate wheth...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Shikha Upreti Tapas Chandra Nag Madhumita P Ghosh Source Type: research
Short peptides derived from pigment epithelium-derived factor attenuate retinal ischemia reperfusion injury through inhibition of apoptosis and inflammatory response in rats
Exp Eye Res. 2023 Dec 4:109743. doi: 10.1016/j.exer.2023.109743. Online ahead of print.ABSTRACTPigment epithelium-derived factor (PEDF) is widely recognized as a neuroprotective factor expressed in the retina and has shown therapeutic potential in several retinal diseases. Our study aimed to identify the neuroprotective fragment in PEDF and investigate its protective activity in retinas under ischemia-reperfusion (IR) condition. We synthesized a series of shorter synthetic peptides, 6-mer (Ser93-Gln98) and its d-form variant (6 dS) derived from the 44-mer (Val78-Thr121; a PEDF neurotrophic fragment), to determine their cyt...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Ming-Hui Sun Tsung-Chuan Ho Shu-I Yeh Show-Li Chen Yeou-Ping Tsao Source Type: research
Reduction of lens size in PAX6-related aniridia
Exp Eye Res. 2023 Dec 5;238:109746. doi: 10.1016/j.exer.2023.109746. Online ahead of print.ABSTRACTHeterozygous mutation of PAX6 in humans leads to congenital aniridia (OMIM 106210) which is typified by congenital iris and foveal defects, and later onset glaucoma, aniridic keratopathy, and cataract. Mice heterozygous for Pax6 mutations phenocopy many aspects of aniridia including the iris defects, keratopathy and cataract, although Pax6 mutant mice have small lenses, a phenotype which is not typically reported in human aniridia, perhaps due to difficulties in measuring lens diameter during typical ophthalmic examinations a...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Melinda K Duncan Alejandra Daruich Sophie Valleix Dominique Bremond-Gignac Source Type: research
Visualization of choroidal vasculature in pigmented mouse eyes from experimental models of AMD
Exp Eye Res. 2023 Dec 7;238:109741. doi: 10.1016/j.exer.2023.109741. Online ahead of print.ABSTRACTA variety of techniques exist to investigate retinal and choroidal vascular changes in experimental mouse models of human ocular diseases. While all have specific advantages, a method for evaluating the choroidal vasculature in pigmented mouse eyes has been more challenging especially for whole mount visualization and morphometric analysis. Here we report a simple, reliable technique involving bleaching pigment prior to immunostaining the vasculature in whole mounts of pigmented mouse choroids. Eyes from healthy adult pigment...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Imran A Bhutto D Scott McLeod Benjamin R Thomson Gerard A Lutty Malia M Edwards Source Type: research
Trolox aids coenzyme Q < sub > 10 < /sub > in neuroprotection against NMDA induced damage via upregulation of VEGF in rat model of glutamate excitotoxicity
Exp Eye Res. 2023 Dec 4;238:109740. doi: 10.1016/j.exer.2023.109740. Online ahead of print.ABSTRACTGlutamate induced damage to retinal ganglion cells (RGCs) requires tight physiological regulation of the N-methyl-D-aspartate (NMDA) receptors. Previously, studies have demonstrated the neuroprotective abilities of antioxidants like coenzyme Q10 (CoQ10) and vitamin E analogs like α-tocopherol against neuropathies resulting from NMDA insult, but have failed to shed light on the effect of CoQ10 and trolox, a hydrophilic analog of vitamin E, on glaucomatous neurodegeneration. In the current study, we wanted to investigate wheth...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Shikha Upreti Tapas Chandra Nag Madhumita P Ghosh Source Type: research
Short peptides derived from pigment epithelium-derived factor attenuate retinal ischemia reperfusion injury through inhibition of apoptosis and inflammatory response in rats
Exp Eye Res. 2023 Dec 4:109743. doi: 10.1016/j.exer.2023.109743. Online ahead of print.ABSTRACTPigment epithelium-derived factor (PEDF) is widely recognized as a neuroprotective factor expressed in the retina and has shown therapeutic potential in several retinal diseases. Our study aimed to identify the neuroprotective fragment in PEDF and investigate its protective activity in retinas under ischemia-reperfusion (IR) condition. We synthesized a series of shorter synthetic peptides, 6-mer (Ser93-Gln98) and its d-form variant (6 dS) derived from the 44-mer (Val78-Thr121; a PEDF neurotrophic fragment), to determine their cyt...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Ming-Hui Sun Tsung-Chuan Ho Shu-I Yeh Show-Li Chen Yeou-Ping Tsao Source Type: research
Reduction of lens size in PAX6-related aniridia
Exp Eye Res. 2023 Dec 4:109746. doi: 10.1016/j.exer.2023.109746. Online ahead of print.ABSTRACTHeterozygous mutation of PAX6 in humans leads to congenital aniridia (OMIM 106210) which is typified by congenital iris and foveal defects, and later onset glaucoma, aniridic keratopathy, and cataract. Mice heterozygous for Pax6 mutations phenocopy many aspects of aniridia including the iris defects, keratopathy and cataract, although Pax6 mutant mice have small lenses, a phenotype which is not typically reported in human aniridia, perhaps due to difficulties in measuring lens diameter during typical ophthalmic examinations as th...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Melinda K Duncan Alejandra Daruich Sophie Valleix Dominique Bremond-Gignac Source Type: research
Visualization of choroidal vasculature in pigmented mouse eyes from experimental models of AMD
Exp Eye Res. 2023 Dec 4:109741. doi: 10.1016/j.exer.2023.109741. Online ahead of print.ABSTRACTA variety of techniques exist to investigate retinal and choroidal vascular changes in experimental mouse models of human ocular diseases. While all have specific advantages, a method for evaluating the choroidal vasculature in pigmented mouse eyes has been more challenging especially for whole mount visualization and morphometric analysis. Here we report a simple, reliable technique involving bleaching pigment prior to immunostaining the vasculature in whole mounts of pigmented mouse choroids. Eyes from healthy adult pigmented C...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Imran A Bhutto D Scott McLeod Benjamin R Thomson Gerard A Lutty Malia M Edwards Source Type: research
Trolox aids coenzyme Q < sub > 10 < /sub > in neuroprotection against NMDA induced damage via upregulation of VEGF in rat model of glutamate excitotoxicity
Exp Eye Res. 2023 Dec 4:109740. doi: 10.1016/j.exer.2023.109740. Online ahead of print.ABSTRACTGlutamate induced damage to retinal ganglion cells (RGCs) requires tight physiological regulation of the N-methyl-D-aspartate (NMDA) receptors. Previously, studies have demonstrated the neuroprotective abilities of antioxidants like coenzyme Q10 (CoQ10) and vitamin E analogs like α-tocopherol against neuropathies resulting from NMDA insult, but have failed to shed light on the effect of CoQ10 and trolox, a hydrophilic analog of vitamin E, on glaucomatous neurodegeneration. In the current study, we wanted to investigate whether t...
Source: Experimental Eye Research - December 6, 2023 Category: Opthalmology Authors: Shikha Upreti Tapas Chandra Nag Madhumita P Ghosh Source Type: research
Mouse model of radiation retinopathy reveals vascular and neuronal injury
CONCLUSIONS: By histology, vascular leakage at 10 days was followed by increased neuronal apoptosis and loss of neuronal and vascular density. However, in vivo imaging approaches that are commonly used in human patients did not detect pathology in mice.PMID:38052338 | DOI:10.1016/j.exer.2023.109729 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 5, 2023 Category: Opthalmology Authors: Emily Liu Michelle R Tamplin Jurnie Rosius Thomas R Tedeschi Oliver W Gramlich Randy H Kardon Isabella M Grumbach Source Type: research
Mouse model of radiation retinopathy reveals vascular and neuronal injury
CONCLUSIONS: By histology, vascular leakage at 10 days was followed by increased neuronal apoptosis and loss of neuronal and vascular density. However, in vivo imaging approaches that are commonly used in human patients did not detect pathology in mice.PMID:38052338 | DOI:10.1016/j.exer.2023.109729 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 5, 2023 Category: Opthalmology Authors: Emily Liu Michelle R Tamplin Jurnie Rosius Thomas R Tedeschi Oliver W Gramlich Randy H Kardon Isabella M Grumbach Source Type: research
Inhibition of Rho kinase (ROCK) impairs cytoskeletal contractility in human M üller glial cells without effects on cell viability, migration, and extracellular matrix production
In conclusion, pharmacological inhibition of ROCK in Müller cells could be a potentially promising approach to treat epiretinal membranes by preventing cell proliferation, contractility and transdifferentiation, without affecting cell viability.PMID:38043763 | DOI:10.1016/j.exer.2023.109745 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 3, 2023 Category: Opthalmology Authors: Vinicius Moraes de Paiva Roda Rafael Andr é da Silva Paula Veloso Siqueira Gabriela de Jesus Lustoza-Costa Gabri élla Malheiros de Moraes Monique Matsuda D ânia Emi Hamassaki Marinilce Fagundes Santos Source Type: research
Inhibition of Rho kinase (ROCK) impairs cytoskeletal contractility in human M üller glial cells without effects on cell viability, migration, and extracellular matrix production
In conclusion, pharmacological inhibition of ROCK in Müller cells could be a potentially promising approach to treat epiretinal membranes by preventing cell proliferation, contractility and transdifferentiation, without affecting cell viability.PMID:38043763 | DOI:10.1016/j.exer.2023.109745 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 3, 2023 Category: Opthalmology Authors: Vinicius Moraes de Paiva Roda Rafael Andr é da Silva Paula Veloso Siqueira Gabriela de Jesus Lustoza-Costa Gabri élla Malheiros de Moraes Monique Matsuda D ânia Emi Hamassaki Marinilce Fagundes Santos Source Type: research
