Krupple-like factor 4 (KLF4) methylation signature in host cell in active viral keratitis with epithelial manifestation
Exp Eye Res. 2023 Dec 30:109771. doi: 10.1016/j.exer.2023.109771. Online ahead of print.ABSTRACTHSV1 presents as epithelial or stromal keratitis or keratouveitis and can lead to sight-threatening complications. KLF4, a critical transcription factor, and regulator of cell growth and differentiation, is essential in corneal epithelium stratification and homeostasis. Here, we want to understand the epigenetic modification specifically the methylation status of KLF4 in epithelium samples of HSV1 keratitis patients. After obtaining consent, epithelial scrapes were collected from 7 patients with clinically diagnosed HSV1 keratit...
Source: Experimental Eye Research - January 1, 2024 Category: Opthalmology Authors: Vatsala Nidhi Jyoti Sangwan Swati Sood Moumita Mondal Bharti Sangwan Mehak Vohra Abha Gour Umang Mathur Virender Singh Sangwan Manisha Acharya Anil Tiwari Source Type: research
Optimizing the sodium iodate model: Effects of dose, gender, and age
This study aims to establish a less severe yet reproducible regimen by testing low doses of NaIO3 while considering age- and sex-related effects, enabling a broader range of therapeutic evaluations. In this study, young (3-5 months) and old (18-24 months) male and female C57Bl/6J mice were given an intraperitoneal (IP) injection of 15, 20, or 25 mg/kg NaIO3. Damage assessment one week post-injection included in vivo imaging, histological examination, and qRT-PCR analysis. The results revealed that young mice showed no damage at 15 mg/kg IP NaIO3, with varying degrees of damage observed at 20 mg/kg. At 25 mg/kg, most young ...
Source: Experimental Eye Research - December 29, 2023 Category: Opthalmology Authors: Brandon D Anderson Timothy T Lee Brent A Bell Tan Wang Joshua L Dunaief Source Type: research
Identification and cellular localization in Xenopus laevis photoreceptors of three Peripherin-2 family members, Prph2, Rom1 and Gp2l, which arose from gene duplication events in the common ancestors of jawed vertebrates
Exp Eye Res. 2023 Dec 27:109760. doi: 10.1016/j.exer.2023.109760. Online ahead of print.ABSTRACTRod and cone photoreceptors are named for the distinct morphologies of their outer segment organelles, which are either cylindrical or conical, respectively. The morphologies of the stacked disks that comprise the rod and cone outer segments also differ: rod disks are completely sealed and are discontinuous with the plasma membrane, while cone disks remain partially open to the extracellular space. These morphological differences between photoreceptor types are more prominent in non-mammalian vertebrates, whose cones typically p...
Source: Experimental Eye Research - December 29, 2023 Category: Opthalmology Authors: Beatrice M Tam John S Taylor Orson L Moritz Source Type: research
Age and light damage influence Fzd5 regulation of ocular growth-related genes
In this study, we compiled a list of human genes associated with ocular growth abnormalities based on public databases and a literature search. We identified a set of ocular growth-related genes from the list that was altered in the Fzd5 mutant mice by RNAseq analysis at different time points. The Fzd5 regulation of this set of genes appeared to be impacted by age and light damage. Further bioinformatical analysis indicated that these genes are extracellular matrix (ECM)-related; and meanwhile an altered Wnt signaling was detected. Altogether, the data suggest that Fzd5 may regulate ocular growth through regulating ECM rem...
Source: Experimental Eye Research - December 28, 2023 Category: Opthalmology Authors: Shanzhen Peng Mingzhu Guo Cheng Wu Jinsong Liu Bin Zou Yuanyuan Chen Yingchun Su Lei Shi Shiyong Zhu Shujuan Xu Dianlei Guo Rong Ju Lai Wei Yanhong Wei Chunqiao Liu Source Type: research
Age and light damage influence Fzd5 regulation of ocular growth-related genes
In this study, we compiled a list of human genes associated with ocular growth abnormalities based on public databases and a literature search. We identified a set of ocular growth-related genes from the list that was altered in the Fzd5 mutant mice by RNAseq analysis at different time points. The Fzd5 regulation of this set of genes appeared to be impacted by age and light damage. Further bioinformatical analysis indicated that these genes are extracellular matrix (ECM)-related; and meanwhile an altered Wnt signaling was detected. Altogether, the data suggest that Fzd5 may regulate ocular growth through regulating ECM rem...
Source: Experimental Eye Research - December 28, 2023 Category: Opthalmology Authors: Shanzhen Peng Mingzhu Guo Cheng Wu Jinsong Liu Bin Zou Yuanyuan Chen Yingchun Su Lei Shi Shiyong Zhu Shujuan Xu Dianlei Guo Rong Ju Lai Wei Yanhong Wei Chunqiao Liu Source Type: research
Age and light damage influence Fzd5 regulation of ocular growth-related genes
In this study, we compiled a list of human genes associated with ocular growth abnormalities based on public databases and a literature search. We identified a set of ocular growth-related genes from the list that was altered in the Fzd5 mutant mice by RNAseq analysis at different time points. The Fzd5 regulation of this set of genes appeared to be impacted by age and light damage. Further bioinformatical analysis indicated that these genes are extracellular matrix (ECM)-related; and meanwhile an altered Wnt signaling was detected. Altogether, the data suggest that Fzd5 may regulate ocular growth through regulating ECM rem...
Source: Experimental Eye Research - December 28, 2023 Category: Opthalmology Authors: Shanzhen Peng Mingzhu Guo Cheng Wu Jinsong Liu Bin Zou Yuanyuan Chen Yingchun Su Lei Shi Shiyong Zhu Shujuan Xu Dianlei Guo Rong Ju Lai Wei Yanhong Wei Chunqiao Liu Source Type: research
Age and light damage influence Fzd5 regulation of ocular growth-related genes
In this study, we compiled a list of human genes associated with ocular growth abnormalities based on public databases and a literature search. We identified a set of ocular growth-related genes from the list that was altered in the Fzd5 mutant mice by RNAseq analysis at different time points. The Fzd5 regulation of this set of genes appeared to be impacted by age and light damage. Further bioinformatical analysis indicated that these genes are extracellular matrix (ECM)-related; and meanwhile an altered Wnt signaling was detected. Altogether, the data suggest that Fzd5 may regulate ocular growth through regulating ECM rem...
Source: Experimental Eye Research - December 28, 2023 Category: Opthalmology Authors: Shanzhen Peng Mingzhu Guo Cheng Wu Jinsong Liu Bin Zou Yuanyuan Chen Yingchun Su Lei Shi Shiyong Zhu Shujuan Xu Dianlei Guo Rong Ju Lai Wei Yanhong Wei Chunqiao Liu Source Type: research
Age-related changes on physicochemical properties of the artificial vitreous humor: A practical tool for enhancing ex vivo studies
In conclusion, age-dependent A-VH samples were created successfully to use ex vivo method development studies, and the influence of aging on the physicochemical properties of VH was demonstrated as well.PMID:38147936 | DOI:10.1016/j.exer.2023.109762 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 26, 2023 Category: Opthalmology Authors: Simge Zengin Selda Mercan Duygu Tarhan Asl ı Gök Alev Meltem Ercan Source Type: research
Age-related changes on physicochemical properties of the artificial vitreous humor: A practical tool for enhancing ex vivo studies
In conclusion, age-dependent A-VH samples were created successfully to use ex vivo method development studies, and the influence of aging on the physicochemical properties of VH was demonstrated as well.PMID:38147936 | DOI:10.1016/j.exer.2023.109762 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 26, 2023 Category: Opthalmology Authors: Simge Zengin Selda Mercan Duygu Tarhan Asl ı Gök Alev Meltem Ercan Source Type: research
Role of immune inflammation regulated by macrophage in the pathogenesis of age-related macular degeneration
Exp Eye Res. 2023 Dec 23:109770. doi: 10.1016/j.exer.2023.109770. Online ahead of print.ABSTRACTAge-related macular degeneration (AMD) can lead to irreversible impairment of visual function, and the number of patients with AMD has been increasing globally. The immunoinflammatory theory is an important pathogenic mechanism of AMD, with macrophages serving as the primary inflammatory infiltrating cells in AMD lesions. Its powerful immunoinflammatory regulatory function has attracted considerable attention. Herein, we provide an overview of the involvement of macrophage-regulated immunoinflammation in different stages of AMD....
Source: Experimental Eye Research - December 25, 2023 Category: Opthalmology Authors: Qin Zhao Kunbei Lai Source Type: research
TYR mutation in a Chinese population with oculocutaneous albinism: Molecular characteristics and ophthalmic manifestations
In conclusion: The TYR patients exhibited severe ocular phenotypes: the majority (93.8%, 15/16) of them had a moderate vision impairment or worse, and 100% (12/12) had severe grade 4 foveal hypoplasia. These novel findings could provide insight into the understanding of OCA.PMID:38145795 | DOI:10.1016/j.exer.2023.109761 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 25, 2023 Category: Opthalmology Authors: Chonglin Chen Jun Li Bingqi Wang Yinghuan Wang Xinping Yu Source Type: research
Role of immune inflammation regulated by macrophage in the pathogenesis of age-related macular degeneration
Exp Eye Res. 2023 Dec 23:109770. doi: 10.1016/j.exer.2023.109770. Online ahead of print.ABSTRACTAge-related macular degeneration (AMD) can lead to irreversible impairment of visual function, and the number of patients with AMD has been increasing globally. The immunoinflammatory theory is an important pathogenic mechanism of AMD, with macrophages serving as the primary inflammatory infiltrating cells in AMD lesions. Its powerful immunoinflammatory regulatory function has attracted considerable attention. Herein, we provide an overview of the involvement of macrophage-regulated immunoinflammation in different stages of AMD....
Source: Experimental Eye Research - December 25, 2023 Category: Opthalmology Authors: Qin Zhao Kunbei Lai Source Type: research
TYR mutation in a Chinese population with oculocutaneous albinism: Molecular characteristics and ophthalmic manifestations
In conclusion: The TYR patients exhibited severe ocular phenotypes: the majority (93.8%, 15/16) of them had a moderate vision impairment or worse, and 100% (12/12) had severe grade 4 foveal hypoplasia. These novel findings could provide insight into the understanding of OCA.PMID:38145795 | DOI:10.1016/j.exer.2023.109761 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 25, 2023 Category: Opthalmology Authors: Chonglin Chen Jun Li Bingqi Wang Yinghuan Wang Xinping Yu Source Type: research
Circular RNA expression profile identifies potential circulating biomarkers for keratoconus
In conclusion, the expression of circRNAs showed a relationship with KC, with four significantly differentially expressed circRNAs demonstrating potential as biomarkers for the disease.PMID:38142763 | DOI:10.1016/j.exer.2023.109759 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 24, 2023 Category: Opthalmology Authors: Xiao-Dan Hao Hai-Pai Gong Fei Li Sheng-Wei Ren Pei-Feng Li Source Type: research
Aurora kinase B disruption suppresses pathological retinal angiogenesis by affecting cell cycle progression
CONCLUSION: In conclusion, AURKB significantly influenced pathological retinal angiogenesis, thereby presenting a promising therapeutic target in ocular neovascular diseases.PMID:38142764 | DOI:10.1016/j.exer.2023.109753 (Source: Experimental Eye Research)
Source: Experimental Eye Research - December 24, 2023 Category: Opthalmology Authors: Weiye Zhu Xiao Gui Yukun Zhou Xin Gao Rui Zhang Qing Li Haorui Zhang Jiawei Zhao Xiao Cui Guangping Gao Huipeng Tang Chenyang Huan Dongyan Pan Hongyuan Song Zhe Zhou Wei Shen Source Type: research
