Stop codon variant in EFEMP1 is associated with primary open-angle glaucoma due to impaired regulation of aqueous humor outflow
This study contributes to the understanding of the important role of fibulin-3 in TM pathophysiology and provides a new possible POAG therapeutic approach.PMID:38467175 | DOI:10.1016/j.exer.2024.109859 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 11, 2024 Category: Opthalmology Authors: Junkai Tan Suping Cai Xiaolin Luo Qiang Li Yanbing Chen Zijie Chen Yukai Mao Guo Liu Mingming Yang Xuyang Liu Source Type: research
Optical development in the murine eye lens of accelerated senescence-prone SAMP8 and senescence-resistant SAMR1 strains
Exp Eye Res. 2024 Mar 9:109858. doi: 10.1016/j.exer.2024.109858. Online ahead of print.ABSTRACTThe eye lens is responsible for focusing objects at various distances onto the retina and its refractive power is determined by its surface curvature as well as its internal gradient refractive index (GRIN). The lens continues to grow with age resulting in changes to the shape and to the GRIN profile. The present study aims to investigate how the ageing process may influence lens optical development. Murine lenses of accelerated senescence-prone strain (SAMP8) aged from 4 to 50 weeks; senescence-resistant strain (SAMR1) aged from...
Source: Experimental Eye Research - March 11, 2024 Category: Opthalmology Authors: Kehao Wang Yutian Pu Leran Chen Masato Hoshino Kentaro Uesugi Naoto Yagi Xiaoyong Chen Yuu Usui Akira Hanashima Ken Hashimoto Satoshi Mohri Barbara K Pierscionek Source Type: research
Sphingolipid biosynthetic inhibitor L-cycloserine prevents oxidative-stress-mediated death in an in vitro model of photoreceptor-derived 661W cells
This study investigates the effect of L-Cycloserine, a small molecule inhibitor of ceramide biosynthesis, on sphingolipid metabolism and the protection of photoreceptor-derived 661 W cells from oxidative stress. The results demonstrate that treatment with L-Cycloserine, an inhibitor of Serine palmitoyl transferase (SPT), markedly decreases bioactive ceramide and associated sphingolipids in 661 W cells. A nontoxic dose of L-Cycloserine can provide substantial protection of 661 W cells against H2O2-induced oxidative stress by reversing the increase in ceramide level observed under oxidative stress conditions. Analysis of var...
Source: Experimental Eye Research - March 9, 2024 Category: Opthalmology Authors: Faiza Tahia Sandip K Basu Amanda Prislovsky Koushik Mondal Dejian Ma Harry Kochat Kennard Brown Daniel J Stephenson Charles E Chalfant Nawajes Mandal Source Type: research
Sphingolipid biosynthetic inhibitor L-cycloserine prevents oxidative-stress-mediated death in an in vitro model of photoreceptor-derived 661W cells
This study investigates the effect of L-Cycloserine, a small molecule inhibitor of ceramide biosynthesis, on sphingolipid metabolism and the protection of photoreceptor-derived 661 W cells from oxidative stress. The results demonstrate that treatment with L-Cycloserine, an inhibitor of Serine palmitoyl transferase (SPT), markedly decreases bioactive ceramide and associated sphingolipids in 661 W cells. A nontoxic dose of L-Cycloserine can provide substantial protection of 661 W cells against H2O2-induced oxidative stress by reversing the increase in ceramide level observed under oxidative stress conditions. Analysis of var...
Source: Experimental Eye Research - March 9, 2024 Category: Opthalmology Authors: Faiza Tahia Sandip K Basu Amanda Prislovsky Koushik Mondal Dejian Ma Harry Kochat Kennard Brown Daniel J Stephenson Charles E Chalfant Nawajes Mandal Source Type: research
Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation
This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-HConEpiC) were incubated under hyperosmolar (400-500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Alfredo Dom ínguez-López Marta Blanco-V ázquez Andr és Ángel Calderón-García Carmen Garc ía-Vázquez Mar ía J González-García Margarita Calonge Amalia Enr íquez-de-Salamanca Source Type: research
Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGF β Ameliorates high myopia-induced ocular hypertension
In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the effect of YAP/TGF-β signaling pathway in TM pathogenesis induced by high myopia. Moreover, we performed stretching treatment on primary trabecular meshwork (TM) cells to simulate the mechanical environment of high myopia. It was found that stretching treatment disrupted the cytoskeleton, decreased phagocytic function, enhanced ECM remodeling, and promoted cell apoptosis. The experim...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Qianwen Bu Hai Zhu Guangliang Cao Ganyu Gong Ying Su Qingshu Ge Wei Zhu Zongyi Li Xiaojing Pan Source Type: research
Oleic acid induces lipogenesis and NLRP3 inflammasome activation in organotypic mouse meibomian gland and human meibomian gland epithelial cells
Exp Eye Res. 2024 Mar 5;241:109851. doi: 10.1016/j.exer.2024.109851. Online ahead of print.ABSTRACTThe accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it is a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human meibomian gland epithelial cells (HMGECs). Organotypic cultured mouse MG and HMGECs were exposed to OA or combinations with speci...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Ren Liu Mengqian Tu Jianwen Xue Bing Xiao Jing Li Lingyi Liang Source Type: research
Intraocular pressure across the lifespan of Tg-MYOC < sup > Y437H < /sup > mice
In this study, we compiled the model's intraocular pressure (IOP) data recorded in our laboratory from 2017 to 2023 and selected representative eyes to measure the outflow facility (Cr), a critical parameter indicating the condition of the conventional TM pathway. We found that Tg-MYOCY437H mice aged 4-12 months exhibited significantly higher IOPs than age-matched C57BL/6 mice. Notably, a decline in IOP was observed in Tg-MYOCY437H mice at 17-24 months of age, a phenomenon not attributable to the gene dosage of mutant myocilin. Measurements of the Cr of Tg-MYOCY437H mice indicated that the age-related IOP reduction was not...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Xiaoyan Zhang Gaiping Xi Pengchao Feng Cong Li Markus H Kuehn Wei Zhu Source Type: research
Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation
This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-HConEpiC) were incubated under hyperosmolar (400-500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Alfredo Dom ínguez-López Marta Blanco-V ázquez Andr és Ángel Calderón-García Carmen Garc ía-Vázquez Mar ía J González-García Margarita Calonge Amalia Enr íquez-de-Salamanca Source Type: research
Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGF β Ameliorates high myopia-induced ocular hypertension
In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the effect of YAP/TGF-β signaling pathway in TM pathogenesis induced by high myopia. Moreover, we performed stretching treatment on primary trabecular meshwork (TM) cells to simulate the mechanical environment of high myopia. It was found that stretching treatment disrupted the cytoskeleton, decreased phagocytic function, enhanced ECM remodeling, and promoted cell apoptosis. The experim...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Qianwen Bu Hai Zhu Guangliang Cao Ganyu Gong Ying Su Qingshu Ge Wei Zhu Zongyi Li Xiaojing Pan Source Type: research
Oleic acid induces lipogenesis and NLRP3 inflammasome activation in organotypic mouse meibomian gland and human meibomian gland epithelial cells
Exp Eye Res. 2024 Mar 5:109851. doi: 10.1016/j.exer.2024.109851. Online ahead of print.ABSTRACTThe accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it's a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human meibomian gland epithelial cells (HMGECs). Organotypic cultured mouse MG and HMGECs were exposed to OA or combinations with specific A...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Ren Liu Mengqian Tu Jianwen Xue Bing Xiao Jing Li Lingyi Liang Source Type: research
Intraocular pressure across the lifespan of Tg-MYOC < sup > Y437H < /sup > mice
In this study, we compiled the model's intraocular pressure (IOP) data recorded in our laboratory from 2017 to 2023 and selected representative eyes to measure the outflow facility (Cr), a critical parameter indicating the condition of the conventional TM pathway. We found that Tg-MYOCY437H mice aged 4-12 months exhibited significantly higher IOPs than age-matched C57BL/6 mice. Notably, a decline in IOP was observed in Tg-MYOCY437H mice at 17-24 months of age, a phenomenon not attributable to the gene dosage of mutant myocilin. Measurements of the Cr of Tg-MYOCY437H mice indicated that the age-related IOP reduction was not...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Xiaoyan Zhang Gaiping Xi Pengchao Feng Cong Li Markus H Kuehn Wei Zhu Source Type: research
Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation
This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-HConEpiC) were incubated under hyperosmolar (400-500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Alfredo Dom ínguez-López Marta Blanco-V ázquez Andr és Ángel Calderón-García Carmen Garc ía-Vázquez Mar ía J González-García Margarita Calonge Amalia Enr íquez-de-Salamanca Source Type: research
Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGF β Ameliorates high myopia-induced ocular hypertension
In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the effect of YAP/TGF-β signaling pathway in TM pathogenesis induced by high myopia. Moreover, we performed stretching treatment on primary trabecular meshwork (TM) cells to simulate the mechanical environment of high myopia. It was found that stretching treatment disrupted the cytoskeleton, decreased phagocytic function, enhanced ECM remodeling, and promoted cell apoptosis. The experim...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Qianwen Bu Hai Zhu Guangliang Cao Ganyu Gong Ying Su Qingshu Ge Wei Zhu Zongyi Li Xiaojing Pan Source Type: research
Oleic acid induces lipogenesis and NLRP3 inflammasome activation in organotypic mouse meibomian gland and human meibomian gland epithelial cells
Exp Eye Res. 2024 Mar 5:109851. doi: 10.1016/j.exer.2024.109851. Online ahead of print.ABSTRACTThe accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it's a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human meibomian gland epithelial cells (HMGECs). Organotypic cultured mouse MG and HMGECs were exposed to OA or combinations with specific A...
Source: Experimental Eye Research - March 7, 2024 Category: Opthalmology Authors: Ren Liu Mengqian Tu Jianwen Xue Bing Xiao Jing Li Lingyi Liang Source Type: research
