Polymorphism in F pocket affects peptide selection and stability of type 1 diabetes-associated HLA-B39 allotypes
Eur J Immunol. 2024 Mar 29:e2350683. doi: 10.1002/eji.202350683. Online ahead of print.ABSTRACTHLA-B*39:06, HLA-B*39:01, and HLA-B*38:01 are closely related HLA allotypes differentially associated with type 1 diabetes (T1D) risk and progression. B*39:06 is highly predisposing, while B*39:01 and B*38:01 are weakly predisposing and protective allotypes, respectively. Here, we aimed to decipher molecular mechanisms underlying the differential association of these allotypes with T1D pathogenesis. We addressed peptide binding and conformational stability of HLA-B allotypes using computational and experimental approaches. Comput...
Source: European Journal of Immunology - March 29, 2024 Category: Allergy & Immunology Authors: A W Peshala Amarajeewa Aslihan Özcan Alveena Mukhtiar Xu Ren Qianyu Wang Pemra Ozbek Malgorzata A Garstka Onur Ser çinoğlu Source Type: research
Polymorphism in F pocket affects peptide selection and stability of type 1 diabetes-associated HLA-B39 allotypes
Eur J Immunol. 2024 Mar 29:e2350683. doi: 10.1002/eji.202350683. Online ahead of print.ABSTRACTHLA-B*39:06, HLA-B*39:01, and HLA-B*38:01 are closely related HLA allotypes differentially associated with type 1 diabetes (T1D) risk and progression. B*39:06 is highly predisposing, while B*39:01 and B*38:01 are weakly predisposing and protective allotypes, respectively. Here, we aimed to decipher molecular mechanisms underlying the differential association of these allotypes with T1D pathogenesis. We addressed peptide binding and conformational stability of HLA-B allotypes using computational and experimental approaches. Comput...
Source: European Journal of Immunology - March 29, 2024 Category: Allergy & Immunology Authors: A W Peshala Amarajeewa Aslihan Özcan Alveena Mukhtiar Xu Ren Qianyu Wang Pemra Ozbek Malgorzata A Garstka Onur Ser çinoğlu Source Type: research
High sensitivity of host Helios < sup > + < /sup > /Neuropilin-1 < sup > + < /sup > Treg to pretransplant conditioning hampers development of OX40 < sup > bright < /sup > /integrin- β7 < sup > + < /sup > regulatory cells in acute gastrointestinal GvHD
This study sought to compare the behavior of Treg subsets displaying different coexpression patterns of Neuropilin-1 (Nrp1) and Helios, under the influence of gut stress unrelated to hematopoietic stem cell transplantation, pretransplantation conditioning, and posttransplant gastrointestinal acute graft versus host disease (GI-aGvHD). Host CD4+/CD25hi/Foxp3+ Treg cells, identified by flow cytometry, were isolated from various tissues of mice affected by these stressors. Expression of CD25, CTLA-4, CD39, OX40, integrin-β7, LAG3, TGFβ/LAP, granzyme-A, -B, and interleukin-10 was compared in four Treg subsets displaying Heli...
Source: European Journal of Immunology - March 27, 2024 Category: Allergy & Immunology Authors: Nikolett Lupsa Barbara Érsek Csenge B öröczky D ávid Kis Eszter Szarka Katalin Lumniczky G éza Sáfrány Zolt án S Zádori Árpád Szöőr Edit I Buz ás Zolt án Pós Source Type: research
High sensitivity of host Helios < sup > + < /sup > /Neuropilin-1 < sup > + < /sup > Treg to pretransplant conditioning hampers development of OX40 < sup > bright < /sup > /integrin- β7 < sup > + < /sup > regulatory cells in acute gastrointestinal GvHD
This study sought to compare the behavior of Treg subsets displaying different coexpression patterns of Neuropilin-1 (Nrp1) and Helios, under the influence of gut stress unrelated to hematopoietic stem cell transplantation, pretransplantation conditioning, and posttransplant gastrointestinal acute graft versus host disease (GI-aGvHD). Host CD4+/CD25hi/Foxp3+ Treg cells, identified by flow cytometry, were isolated from various tissues of mice affected by these stressors. Expression of CD25, CTLA-4, CD39, OX40, integrin-β7, LAG3, TGFβ/LAP, granzyme-A, -B, and interleukin-10 was compared in four Treg subsets displaying Heli...
Source: European Journal of Immunology - March 27, 2024 Category: Allergy & Immunology Authors: Nikolett Lupsa Barbara Érsek Csenge B öröczky D ávid Kis Eszter Szarka Katalin Lumniczky G éza Sáfrány Zolt án S Zádori Árpád Szöőr Edit I Buz ás Zolt án Pós Source Type: research
High sensitivity of host Helios < sup > + < /sup > /Neuropilin-1 < sup > + < /sup > Treg to pretransplant conditioning hampers development of OX40 < sup > bright < /sup > /integrin- β7 < sup > + < /sup > regulatory cells in acute gastrointestinal GvHD
This study sought to compare the behavior of Treg subsets displaying different coexpression patterns of Neuropilin-1 (Nrp1) and Helios, under the influence of gut stress unrelated to hematopoietic stem cell transplantation, pretransplantation conditioning, and posttransplant gastrointestinal acute graft versus host disease (GI-aGvHD). Host CD4+/CD25hi/Foxp3+ Treg cells, identified by flow cytometry, were isolated from various tissues of mice affected by these stressors. Expression of CD25, CTLA-4, CD39, OX40, integrin-β7, LAG3, TGFβ/LAP, granzyme-A, -B, and interleukin-10 was compared in four Treg subsets displaying Heli...
Source: European Journal of Immunology - March 27, 2024 Category: Allergy & Immunology Authors: Nikolett Lupsa Barbara Érsek Csenge B öröczky D ávid Kis Eszter Szarka Katalin Lumniczky G éza Sáfrány Zolt án S Zádori Árpád Szöőr Edit I Buz ás Zolt án Pós Source Type: research
Age-related transcript changes in type I interferon signaling in children and adolescents with long COVID
Eur J Immunol. 2024 Mar 24:e2350682. doi: 10.1002/eji.202350682. Online ahead of print.ABSTRACTSARS-CoV-2 typically causes mild symptoms in children, but evidence suggests that persistent immunopathological changes may lead to long COVID (LC). To explore the interplay between LC and innate immunity, we assessed the type I interferon (IFN-I) response in children and adolescents with LC symptoms (LC; n = 28). This was compared with age-matched SARS-CoV-2 recovered participants without LC symptoms (MC; n = 28) and healthy controls (HC; n = 18). We measured the mRNA expression of IFN-I (IFN-α/β/ε/ω), IFN-I receptor (IFNAR1...
Source: European Journal of Immunology - March 24, 2024 Category: Allergy & Immunology Authors: Matteo Fracella Enrica Mancino Raffaella Nenna Chiara Virgillito Federica Frasca Alessandra D'Auria Leonardo Sorrentino Laura Petrarca Domenico La Regina Luigi Matera Greta Di Mattia Beniamino Caputo Guido Antonelli Alessandra Pierangeli Raphael P Viscidi Source Type: research
Age-related transcript changes in type I interferon signaling in children and adolescents with long COVID
Eur J Immunol. 2024 Mar 24:e2350682. doi: 10.1002/eji.202350682. Online ahead of print.ABSTRACTSARS-CoV-2 typically causes mild symptoms in children, but evidence suggests that persistent immunopathological changes may lead to long COVID (LC). To explore the interplay between LC and innate immunity, we assessed the type I interferon (IFN-I) response in children and adolescents with LC symptoms (LC; n = 28). This was compared with age-matched SARS-CoV-2 recovered participants without LC symptoms (MC; n = 28) and healthy controls (HC; n = 18). We measured the mRNA expression of IFN-I (IFN-α/β/ε/ω), IFN-I receptor (IFNAR1...
Source: European Journal of Immunology - March 24, 2024 Category: Allergy & Immunology Authors: Matteo Fracella Enrica Mancino Raffaella Nenna Chiara Virgillito Federica Frasca Alessandra D'Auria Leonardo Sorrentino Laura Petrarca Domenico La Regina Luigi Matera Greta Di Mattia Beniamino Caputo Guido Antonelli Alessandra Pierangeli Raphael P Viscidi Source Type: research
Age-related transcript changes in type I interferon signaling in children and adolescents with long COVID
Eur J Immunol. 2024 Mar 24:e2350682. doi: 10.1002/eji.202350682. Online ahead of print.ABSTRACTSARS-CoV-2 typically causes mild symptoms in children, but evidence suggests that persistent immunopathological changes may lead to long COVID (LC). To explore the interplay between LC and innate immunity, we assessed the type I interferon (IFN-I) response in children and adolescents with LC symptoms (LC; n = 28). This was compared with age-matched SARS-CoV-2 recovered participants without LC symptoms (MC; n = 28) and healthy controls (HC; n = 18). We measured the mRNA expression of IFN-I (IFN-α/β/ε/ω), IFN-I receptor (IFNAR1...
Source: European Journal of Immunology - March 24, 2024 Category: Allergy & Immunology Authors: Matteo Fracella Enrica Mancino Raffaella Nenna Chiara Virgillito Federica Frasca Alessandra D'Auria Leonardo Sorrentino Laura Petrarca Domenico La Regina Luigi Matera Greta Di Mattia Beniamino Caputo Guido Antonelli Alessandra Pierangeli Raphael P Viscidi Source Type: research
cDC2 plasticity and acquisition of a DC3-like phenotype mediated by IL-6 and PGE2 in a patient-derived colorectal cancer organoids model
In this study, we use our recently developed 3D DC-tumor co-culture system to investigate how patient-derived primary and metastatic CRC organoids modulate cDC2 phenotype and function. We first demonstrate that our collagen-based system displays extensive interaction between cDC2 and tumor organoids. Interestingly, we show that tumor-corrupted cDC2 shift toward a CD14+ population with defective expression of maturation markers, an intermediate phenotype positioned between cDC2 and monocytes, and impaired T-cell activating abilities. This phenotype aligns with the newly defined DC3 (CD14+ CD1c+ CD163+) subset. Remarkably, a...
Source: European Journal of Immunology - March 21, 2024 Category: Allergy & Immunology Authors: Beatriz Subtil Iris A E van der Hoorn Jorge Cuenca-Escalona Anouk M D Becker Mar Alvarez-Begue Kirti K Iyer Jorien Janssen Tom van Oorschot Dennis Poel Mark A J Gorris Koen van den Dries Alessandra Cambi Daniele V F Tauriello I Jolanda M de Vries Source Type: research
cDC2 plasticity and acquisition of a DC3-like phenotype mediated by IL-6 and PGE2 in a patient-derived colorectal cancer organoids model
In this study, we use our recently developed 3D DC-tumor co-culture system to investigate how patient-derived primary and metastatic CRC organoids modulate cDC2 phenotype and function. We first demonstrate that our collagen-based system displays extensive interaction between cDC2 and tumor organoids. Interestingly, we show that tumor-corrupted cDC2 shift toward a CD14+ population with defective expression of maturation markers, an intermediate phenotype positioned between cDC2 and monocytes, and impaired T-cell activating abilities. This phenotype aligns with the newly defined DC3 (CD14+ CD1c+ CD163+) subset. Remarkably, a...
Source: European Journal of Immunology - March 21, 2024 Category: Allergy & Immunology Authors: Beatriz Subtil Iris A E van der Hoorn Jorge Cuenca-Escalona Anouk M D Becker Mar Alvarez-Begue Kirti K Iyer Jorien Janssen Tom van Oorschot Dennis Poel Mark A J Gorris Koen van den Dries Alessandra Cambi Daniele V F Tauriello I Jolanda M de Vries Source Type: research
cDC2 plasticity and acquisition of a DC3-like phenotype mediated by IL-6 and PGE2 in a patient-derived colorectal cancer organoids model
In this study, we use our recently developed 3D DC-tumor co-culture system to investigate how patient-derived primary and metastatic CRC organoids modulate cDC2 phenotype and function. We first demonstrate that our collagen-based system displays extensive interaction between cDC2 and tumor organoids. Interestingly, we show that tumor-corrupted cDC2 shift toward a CD14+ population with defective expression of maturation markers, an intermediate phenotype positioned between cDC2 and monocytes, and impaired T-cell activating abilities. This phenotype aligns with the newly defined DC3 (CD14+ CD1c+ CD163+) subset. Remarkably, a...
Source: European Journal of Immunology - March 21, 2024 Category: Allergy & Immunology Authors: Beatriz Subtil Iris A E van der Hoorn Jorge Cuenca-Escalona Anouk M D Becker Mar Alvarez-Begue Kirti K Iyer Jorien Janssen Tom van Oorschot Dennis Poel Mark A J Gorris Koen van den Dries Alessandra Cambi Daniele V F Tauriello I Jolanda M de Vries Source Type: research
Intestinal tissue-resident memory T cells maintain distinct identity from circulating memory T cells after in vitro restimulation
Eur J Immunol. 2024 Mar 19:e2350873. doi: 10.1002/eji.202350873. Online ahead of print.ABSTRACTResident memory T (TRM ) cells have been recently established as an important subset of memory T cells that provide early and essential protection against reinfection in the absence of circulating memory T cells. Recent findings showing that TRM expand in vivo after repeated antigenic stimulation indicate that these memory T cells are not terminally differentiated. This suggests an opportunity for in vitro TRM expansion to apply in an immunotherapy setting. However, it has also been shown that TRM may not maintain their identity ...
Source: European Journal of Immunology - March 19, 2024 Category: Allergy & Immunology Authors: Ammarina Beumer-Chuwonpad Felix M Behr Floris P J van Alphen Natasja A M Kragten Arie J Hoogendijk Maartje van den Biggelaar Klaas P J M van Gisbergen Source Type: research
Oligonucleotide library screening for identification of virus-specific T-cell receptors
Eur J Immunol. 2024 Mar 19:e2350908. doi: 10.1002/eji.202350908. Online ahead of print.ABSTRACTWe demonstrate an optimized oligonucleotide library-based approach for the identification of virus-reactive T-cell receptors using Epstein-Barr virus as an example. HEK293T served as antigen-presenting cells and were co-cultured with human T cells that were transduced with T-cell receptors in question. T-cell activation was detected by CD137 expression.PMID:38501894 | DOI:10.1002/eji.202350908 (Source: European Journal of Immunology)
Source: European Journal of Immunology - March 19, 2024 Category: Allergy & Immunology Authors: Marthe-Lina Welters Serena Stadler Vasiliki Anastasopoulou Lars Bullinger Matthias Leisegang Thomas Kammert öns Carlotta Welters Leo Hansmann Source Type: research
Intestinal tissue-resident memory T cells maintain distinct identity from circulating memory T cells after in vitro restimulation
Eur J Immunol. 2024 Mar 19:e2350873. doi: 10.1002/eji.202350873. Online ahead of print.ABSTRACTResident memory T (TRM ) cells have been recently established as an important subset of memory T cells that provide early and essential protection against reinfection in the absence of circulating memory T cells. Recent findings showing that TRM expand in vivo after repeated antigenic stimulation indicate that these memory T cells are not terminally differentiated. This suggests an opportunity for in vitro TRM expansion to apply in an immunotherapy setting. However, it has also been shown that TRM may not maintain their identity ...
Source: European Journal of Immunology - March 19, 2024 Category: Allergy & Immunology Authors: Ammarina Beumer-Chuwonpad Felix M Behr Floris P J van Alphen Natasja A M Kragten Arie J Hoogendijk Maartje van den Biggelaar Klaas P J M van Gisbergen Source Type: research
Oligonucleotide library screening for identification of virus-specific T-cell receptors
Eur J Immunol. 2024 Mar 19:e2350908. doi: 10.1002/eji.202350908. Online ahead of print.ABSTRACTWe demonstrate an optimized oligonucleotide library-based approach for the identification of virus-reactive T-cell receptors using Epstein-Barr virus as an example. HEK293T served as antigen-presenting cells and were co-cultured with human T cells that were transduced with T-cell receptors in question. T-cell activation was detected by CD137 expression.PMID:38501894 | DOI:10.1002/eji.202350908 (Source: European Journal of Immunology)
Source: European Journal of Immunology - March 19, 2024 Category: Allergy & Immunology Authors: Marthe-Lina Welters Serena Stadler Vasiliki Anastasopoulou Lars Bullinger Matthias Leisegang Thomas Kammert öns Carlotta Welters Leo Hansmann Source Type: research
