Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages
We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.PMID:38361219 | DOI:10.1002/eji.202350515 (Source: European Journal of Immunology)
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Sara J Thygesen Sabrina S Burgener Prerna Mudai Mercedes Monteleone Dave Boucher Vitaliya Sagulenko Kate Schroder Katryn J Stacey Source Type: research
Keratinocytes in the pathogenesis, phenotypic switch, and relapse of psoriasis
Eur J Immunol. 2024 Feb 14:e2250279. doi: 10.1002/eji.202250279. Online ahead of print.ABSTRACTAlthough biologics have achieved tremendous success in the treatment of psoriasis and revolutionized the clinical management of the disease, certain issues arise during treatments, including the phenotypic switch from psoriasis to other skin disorders and the recurrence of psoriasis after the cessation of biologic treatment. Here we provide a concise overview of the roles of keratinocytes in the pathogenesis of psoriasis, elucidate the involvement of keratinocytes in the phenotypic switch and relapse of psoriasis, and address the...
Source: European Journal of Immunology - February 15, 2024 Category: Allergy & Immunology Authors: Xinxin Wang Yuping Lai Source Type: research
Controlled WASp activity regulates the proliferative response for Treg cell differentiation in the thymus
Eur J Immunol. 2024 Feb 14:e2350450. doi: 10.1002/eji.202350450. Online ahead of print.ABSTRACTThe Wiskott-Aldrich syndrome protein (WASp) regulates actin cytoskeletal dynamics and function of hematopoietic cells. Mutations in the WAS gene lead to two different syndromes; Wiskott-Aldrich syndrome (WAS) caused by loss-of-function mutations, and X-linked neutropenia (XLN) caused by gain-of-function mutations. We previously showed that WASp-deficient mice have a decreased number of regulatory T (Treg) cells in the thymus and the periphery. We here evaluated the impact of WASp mutations on Treg cells in the thymus of WAS and X...
Source: European Journal of Immunology - February 15, 2024 Category: Allergy & Immunology Authors: Larissa Vasconcelos-Fontes Rhaissa C Vieira Minghui He Rafaella Ferreira-Reis Arnon Dias Jurberg Daniella Ar êas Mendes-da-Cruz John Andersson Vinicius Cotta-de-Almeida Lisa S Westerberg Source Type: research
Notch signaling in adipose tissue macrophages prevents diet-induced inflammation and metabolic dysregulation
Eur J Immunol. 2024 Feb 9:e2350669. doi: 10.1002/eji.202350669. Online ahead of print.ABSTRACTThe importance of macrophages in adipose tissue (AT) homeostasis and inflammation is well established. However, the potential cues that regulate their function remain incompletely understood. To bridge this important gap, we sought to characterize novel pathways involved using a mouse model of diet-induced obesity. By performing transcriptomics analysis of AT macrophages (ATMs), we found that late-stage ATMs from high-fat diet mice presented with perturbed Notch signaling accompanied by robust proinflammatory and metabolic changes...
Source: European Journal of Immunology - February 10, 2024 Category: Allergy & Immunology Authors: Eleni Siouti Maria Salagianni Maria Manioudaki Eleftherios Pavlos Apostolos Klinakis Ioanna-Evdokia Galani Evangelos Andreakos Source Type: research
Induction of a memory-like CD4 < sup > + < /sup > T-cell phenotype by airway smooth muscle cells
Eur J Immunol. 2024 Feb 9:e2249800. doi: 10.1002/eji.202249800. Online ahead of print.ABSTRACTIn asthma, CD4+ T-cell interaction with airway smooth muscle (ASM) may enhance its contractile properties and promote its proliferation. However, less is known about the effects of this interaction on T cells. To explore the consequences of interaction of CD4+ T cells with ASM we placed the cells in co-culture and analyzed the phenotypic and functional changes in the T cells. Effector status as well as cytokine expression was assessed by flow cytometry. An increase in CD45RA- CD45RO+ memory T cells was observed after co-culture; h...
Source: European Journal of Immunology - February 9, 2024 Category: Allergy & Immunology Authors: Joyce H Jang Michael Zhou Kosuke Makita Rui Sun Mikal El-Hajjar Gregory Fonseca Anne-Marie Lauzon James G Martin Source Type: research
Regulation of autoimmune-mediated neuroinflammation by endothelial cells
Eur J Immunol. 2024 Feb 9:e2350482. doi: 10.1002/eji.202350482. Online ahead of print.ABSTRACTThe CNS has traditionally been considered an immune-privileged organ, but recent studies have identified a plethora of immune cells in the choroid plexus, meninges, perivascular spaces, and cribriform plate. Although those immune cells are crucial for the maintenance of CNS homeostasis and for neural protection against infections, they can lead to neuroinflammation in some circumstances. The blood and the lymphatic vasculatures exhibit distinct structural and molecular features depending on their location in the CNS, greatly influ...
Source: European Journal of Immunology - February 9, 2024 Category: Allergy & Immunology Authors: Neil Kallal Stephanie Hugues Laure Garnier Source Type: research
EFIS Ukraine Mobility Initiative
Eur J Immunol. 2024 Feb 5:e2470023. doi: 10.1002/eji.202470023. Online ahead of print.NO ABSTRACTPMID:38314673 | DOI:10.1002/eji.202470023 (Source: European Journal of Immunology)
Source: European Journal of Immunology - February 5, 2024 Category: Allergy & Immunology Source Type: research
The "Domino effect" in MASLD: The inflammatory cascade of steatohepatitis
Eur J Immunol. 2024 Feb 5:e2149641. doi: 10.1002/eji.202149641. Online ahead of print.ABSTRACTMetabolic dysfunction-associated steatotic liver disease (MASLD) is an increasingly common complication of obesity, affecting over a quarter of the global adult population. A key event in the pathophysiology of MASLD is the development of metabolic-associated steatohepatitis (MASH), which greatly increases the chances of developing cirrhosis and hepatocellular carcinoma. The underlying cause of MASH is multifactorial, but accumulating evidence indicates that the inflammatory process in the hepatic microenvironment typically follow...
Source: European Journal of Immunology - February 5, 2024 Category: Allergy & Immunology Authors: Karlo Mladeni ć Maja Lenarti ć Sonja Marinovi ć Bojan Poli ć Felix M Wensveen Source Type: research
Fcgr2b and Fcgr3 are the major genetic factors for cartilage antibody-induced arthritis, overriding the effect of Hc encoding complement C5
In this study, we used a cartilage antibody-induced arthritis (CAIA) model in which arthritis develops within a 12-h timeframe, to determine the relative importance of FCGRs and C5 (Hc). In CAIA, inhibiting or deleting FCGR3 substantially hindered arthritis development, underscoring the crucial role of this receptor. Blocking FCGR3 also reduced the levels of FCGR4, and vice versa. When employing an IgG1 arthritogenic cocktail that exclusively interacts with FCGR2B and FCGR3, joint inflammation was promptly initiated in Fcgr2b-- mice but not in Fcgr3-- mice, suggesting that FCGR3 is sufficient for CAIA development. Regardin...
Source: European Journal of Immunology - February 5, 2024 Category: Allergy & Immunology Authors: Zhongwei Xu Àlex Moreno-Giró Danxia Zhao Alexander Kr ämer Rajan Kumar Pandey Bingze Xu Susanna L Lundstr öm Rikard Holmdahl Source Type: research
Disease-specific plasma protein profiles in patients with fever after traveling to tropical areas
In this study, we measured 49 proteins in the plasma of 124 patients with fever after travel to tropical or subtropical regions. The patients had confirmed diagnoses of either malaria, dengue fever, influenza, bacterial respiratory tract infection, or bacterial gastroenteritis, representing the most common etiologies. We used multivariate and machine learning methods to identify combinations of proteins that contributed to distinguishing infected patients from healthy controls, and each other. Malaria displayed the most unique protein signature, indicating a strong immunoregulatory response with high levels of IL10, sTNFRI...
Source: European Journal of Immunology - February 3, 2024 Category: Allergy & Immunology Authors: Christopher Sundling Victor Yman Zaynab Mousavian Sina Angenendt Fariba Foroogh Ellen von Horn Maximilian Julius Lautenbach Johan Grunewald Anna F ärnert Klara Sond én Source Type: research
Single-cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation
Eur J Immunol. 2024 Feb 2:e2350660. doi: 10.1002/eji.202350660. Online ahead of print.ABSTRACTThawing of viably frozen human tissue T cells, ILCs, and NK cells and subsequent single-cell RNA sequencing reveals that recovery of cellular subclusters is variably impacted. While freeze-thawing does not alter the transcriptional profiles of cells, it upregulates genes and gene pathways associated with stress and activation.PMID:38304946 | DOI:10.1002/eji.202350660 (Source: European Journal of Immunology)
Source: European Journal of Immunology - February 2, 2024 Category: Allergy & Immunology Authors: Christopher T Stamper Anne Marchalot Christopher A Tibbitt Whitney Weigel Mattias Jangard Jakob Theorell Jenny Mj ösberg Source Type: research
Actinin-4 controls survival signaling in B cells by limiting the lateral mobility of B-cell antigen receptors
Eur J Immunol. 2024 Feb 1:e2350774. doi: 10.1002/eji.202350774. Online ahead of print.ABSTRACTThe structure and dynamics of F-actin networks in the cortical area of B cells control the signal efficiency of B-cell antigen receptors (BCRs). Although antigen-induced signaling has been studied extensively, the role of cortical F-actin in antigen-independent tonic BCR signaling is less well understood. Because these signals are essential for the survival of B cells and are consequently exploited by several B-cell lymphomas, we assessed how the cortical F-actin structure influences tonic BCR signal transduction. We employed gene...
Source: European Journal of Immunology - February 1, 2024 Category: Allergy & Immunology Authors: Saed Alsouri Ashley Ambrose Nikolaos Mougios Nadia Paglilla Florian Mayr Kate Choi Jens Loeber Bj örn Chapuy Bj örn Haeupl Felipe Opazo Thomas Oellerich Michael Gold Michael Engelke Source Type: research
Composition of fatty acids in a high-fat diet affects adipose tissue inflammation by inducing calreticulin on adipocytes and activating group 1 innate lymphoid cells
In this study, we examined whether the composition of fatty acids affected adipose inflammatory responses induced by an HFD. Mice were fed a stearic acid (C18:0)-rich HFD (HFD-S) or a linoleic acid (C18:2)-rich HFD (HFD-L). HFD-L-fed mice showed significant obesity compared with HFD-S-fed mice. Visceral and subcutaneous fat pads were enlarged and contained more NK1.1+ KLRG1+ cells, indicating that G1-ILCs were activated in HFD-L-fed mice. We examined early changes in adipose tissues during the first week of HFD intake, and found that mice fed HFD-L showed increased levels of NK1.1+ CD11b+ KLRG1+ cells in adipose tissues. I...
Source: European Journal of Immunology - January 28, 2024 Category: Allergy & Immunology Authors: Kazunori Matsumura Taizo Mori Taeko Dohi Yuki I Kawamura Satoshi Takaki Source Type: research
Nanoparticles for stimulation of neutrophil extracellular trap-mediated immunity
Eur J Immunol. 2024 Jan 26:e2350582. doi: 10.1002/eji.202350582. Online ahead of print.ABSTRACTNeutrophil extracellular traps (NETs) have been identified as triggers for a self-limited inflammatory reaction upon contact with nanoparticles within our bodies. This typically results in entrapping potentially harmful nano- or micro-objects following an immune burst. The demand for potent adjuvants has led to research on particulate-based adjuvants, particularly those that act via NET formation. Various particles, including hydrophobic nanoparticles, needle-like microparticles, and other natural and artificial crystals, have be...
Source: European Journal of Immunology - January 27, 2024 Category: Allergy & Immunology Authors: Anna Vaseruk Galyna Bila Rostyslav Bilyy Source Type: research
PIEZO1-mediated mechanosensing governs NK-cell killing efficiency and infiltration in three-dimensional matrices
In conclusion, our findings demonstrate that PIEZO1-mediated mechanosensing is crucial for NK killing functions, highlighting the role of mechanosensing in NK-cell killing efficiency under 3D physiological conditions and the influence of environmental physical cues on NK-cell functions.PMID:38279603 | DOI:10.1002/eji.202350693 (Source: European Journal of Immunology)
Source: European Journal of Immunology - January 27, 2024 Category: Allergy & Immunology Authors: Archana K Yanamandra Jingnan Zhang Galia Montalvo Xiangda Zhou Doreen Biedenweg Renping Zhao Shulagna Sharma Markus Hoth Franziska Lautenschl äger Oliver Otto Ar ánzazu Del Campo Bin Qu Source Type: research
