Upregulation of IL4-induced gene 1 enzyme by B2 cells during melanoma progression impairs their antitumor properties
Eur J Immunol. 2024 Feb 24:e2350615. doi: 10.1002/eji.202350615. Online ahead of print.ABSTRACTB cells present in human cutaneous melanoma have been associated with protective or detrimental effects on disease progression according to their phenotype. By using the RET model of spontaneous melanoma and adoptive transfer of B16 melanoma cells, we show that immature and follicular B2 (B2-FO) cells exert a protective effect on melanoma progression by promoting the generation of effector memory T cells and limiting the recruitment of polymorphonuclear myeloid-derived suppressor cells. Unfortunately, this beneficial effect progr...
Source: European Journal of Immunology - February 24, 2024 Category: Allergy & Immunology Authors: F ériel Bekkat Malvina Seradj Ren ée Lengagne Fr édéric Fiore Masashi Kato Bruno Lucas Flavia Castellano Val érie Molinier-Frenkel Yolande Richard Armelle Pr évost-Blondel Source Type: research
Upregulation of IL4-induced gene 1 enzyme by B2 cells during melanoma progression impairs their antitumor properties
Eur J Immunol. 2024 Feb 24:e2350615. doi: 10.1002/eji.202350615. Online ahead of print.ABSTRACTB cells present in human cutaneous melanoma have been associated with protective or detrimental effects on disease progression according to their phenotype. By using the RET model of spontaneous melanoma and adoptive transfer of B16 melanoma cells, we show that immature and follicular B2 (B2-FO) cells exert a protective effect on melanoma progression by promoting the generation of effector memory T cells and limiting the recruitment of polymorphonuclear myeloid-derived suppressor cells. Unfortunately, this beneficial effect progr...
Source: European Journal of Immunology - February 24, 2024 Category: Allergy & Immunology Authors: F ériel Bekkat Malvina Seradj Ren ée Lengagne Fr édéric Fiore Masashi Kato Bruno Lucas Flavia Castellano Val érie Molinier-Frenkel Yolande Richard Armelle Pr évost-Blondel Source Type: research
Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming
Eur J Immunol. 2024 Feb 22:e2350872. doi: 10.1002/eji.202350872. Online ahead of print.ABSTRACTLymph node (LN) fine needle aspiration (LN FNA) represents a powerful technique for minimally invasive sampling of human LNs in vivo and has been used effectively to directly study aspects of the human germinal center response. However, systematic deep phenotyping of the cellular populations and cell-free proteins recovered by LN FNA has not been performed. Thus, we studied human cervical LN FNAs as a proof-of-concept and used single-cell RNA-sequencing and proteomic analysis to benchmark this compartment, define the purity of LN...
Source: European Journal of Immunology - February 23, 2024 Category: Allergy & Immunology Authors: Nicholas M Provine Adam Al-Diwani Devika Agarwal Kyla Dooley Amelia Heslington Andrew G Murchison Lucy C Garner Fintan Sheerin Paul Klenerman Sarosh R Irani Source Type: research
Characterization of human CD4 < sup > + < /sup > EOMES < sup > + < /sup > GzmK < sup > + < /sup > T-cell subsets unveils an uncoupling of suppressive functions from IL-10-producing capacities
Eur J Immunol. 2024 Feb 23:e2350675. doi: 10.1002/eji.202350675. Online ahead of print.ABSTRACTHuman CD4+ EOMES+ T cells are heterogeneous and contain Th1-cells, Tr1-cells, and CD4+ CTL. Tr1- cells and non-classical EOMES+ Th1-cells displayed, respectively, anti- and pro-inflammatory cytokine profiles, but both expressed granzyme-K, produced IFN-γ, and suppressed T-cell proliferation. Diffusion map suggested a progressive CD4+ T-cell differentiation from naïve to cytotoxic cells and identified EOMES+ Th1-cells as putative Tr1-cell precursors (pre-Tr1).PMID:38396108 | DOI:10.1002/eji.202350675 (Source: European Journal of Immunology)
Source: European Journal of Immunology - February 23, 2024 Category: Allergy & Immunology Authors: Nadia Pulvirenti Ylenia Silvetri Francesca Clemente Roberto Bosotti Elena Carelli Giorgia Moschetti Paola Gruarin Chiara Vasco Maria Cristina Crosti Maria Lucia Sarnicola Luca Valenti Daniele Prati Sergio Abrignani Jens Geginat Source Type: research
Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming
Eur J Immunol. 2024 Feb 22:e2350872. doi: 10.1002/eji.202350872. Online ahead of print.ABSTRACTLymph node (LN) fine needle aspiration (LN FNA) represents a powerful technique for minimally invasive sampling of human LNs in vivo and has been used effectively to directly study aspects of the human germinal center response. However, systematic deep phenotyping of the cellular populations and cell-free proteins recovered by LN FNA has not been performed. Thus, we studied human cervical LN FNAs as a proof-of-concept and used single-cell RNA-sequencing and proteomic analysis to benchmark this compartment, define the purity of LN...
Source: European Journal of Immunology - February 23, 2024 Category: Allergy & Immunology Authors: Nicholas M Provine Adam Al-Diwani Devika Agarwal Kyla Dooley Amelia Heslington Andrew G Murchison Lucy C Garner Fintan Sheerin Paul Klenerman Sarosh R Irani Source Type: research
Increased infiltration of CD4 < sup > + < /sup > IL-17A < sup > + < /sup > FOXP3 < sup > + < /sup > T cells in Helicobacter pylori-induced gastritis
Eur J Immunol. 2024 Feb 17:e2350662. doi: 10.1002/eji.202350662. Online ahead of print.ABSTRACTHelicobacter pylori is one of the main predisposing factors for gastric cancer, causing chronic inflammation and proper glands atrophy in the gastric mucosa. Although H. pylori-induced inflammation is a key inducer of precancerous lesions in the gastric mucosa, it remains unclear which precise immune cell subsets are responsible for the progression of H. pylori-induced gastritis. Here, we observed an abundance of CD4+ IL-17A+ FOXP3+ T cells exhibiting a Th17-like phenotype within the microenvironment of H. pylori-induced gastriti...
Source: European Journal of Immunology - February 17, 2024 Category: Allergy & Immunology Authors: Yixian Guo Jinnan Chen Yu Huang Shouyu Ke Feng Xie Dan Li Bin Li Hong Lu Source Type: research
Increased infiltration of CD4 < sup > + < /sup > IL-17A < sup > + < /sup > FOXP3 < sup > + < /sup > T cells in Helicobacter pylori-induced gastritis
Eur J Immunol. 2024 Feb 17:e2350662. doi: 10.1002/eji.202350662. Online ahead of print.ABSTRACTHelicobacter pylori is one of the main predisposing factors for gastric cancer, causing chronic inflammation and proper glands atrophy in the gastric mucosa. Although H. pylori-induced inflammation is a key inducer of precancerous lesions in the gastric mucosa, it remains unclear which precise immune cell subsets are responsible for the progression of H. pylori-induced gastritis. Here, we observed an abundance of CD4+ IL-17A+ FOXP3+ T cells exhibiting a Th17-like phenotype within the microenvironment of H. pylori-induced gastriti...
Source: European Journal of Immunology - February 17, 2024 Category: Allergy & Immunology Authors: Yixian Guo Jinnan Chen Yu Huang Shouyu Ke Feng Xie Dan Li Bin Li Hong Lu Source Type: research
Increased infiltration of CD4 < sup > + < /sup > IL-17A < sup > + < /sup > FOXP3 < sup > + < /sup > T cells in Helicobacter pylori-induced gastritis
Eur J Immunol. 2024 Feb 17:e2350662. doi: 10.1002/eji.202350662. Online ahead of print.ABSTRACTHelicobacter pylori is one of the main predisposing factors for gastric cancer, causing chronic inflammation and proper glands atrophy in the gastric mucosa. Although H. pylori-induced inflammation is a key inducer of precancerous lesions in the gastric mucosa, it remains unclear which precise immune cell subsets are responsible for the progression of H. pylori-induced gastritis. Here, we observed an abundance of CD4+ IL-17A+ FOXP3+ T cells exhibiting a Th17-like phenotype within the microenvironment of H. pylori-induced gastriti...
Source: European Journal of Immunology - February 17, 2024 Category: Allergy & Immunology Authors: Yixian Guo Jinnan Chen Yu Huang Shouyu Ke Feng Xie Dan Li Bin Li Hong Lu Source Type: research
Increased infiltration of CD4 < sup > + < /sup > IL-17A < sup > + < /sup > FOXP3 < sup > + < /sup > T cells in Helicobacter pylori-induced gastritis
Eur J Immunol. 2024 Feb 17:e2350662. doi: 10.1002/eji.202350662. Online ahead of print.ABSTRACTHelicobacter pylori is one of the main predisposing factors for gastric cancer, causing chronic inflammation and proper glands atrophy in the gastric mucosa. Although H. pylori-induced inflammation is a key inducer of precancerous lesions in the gastric mucosa, it remains unclear which precise immune cell subsets are responsible for the progression of H. pylori-induced gastritis. Here, we observed an abundance of CD4+ IL-17A+ FOXP3+ T cells exhibiting a Th17-like phenotype within the microenvironment of H. pylori-induced gastriti...
Source: European Journal of Immunology - February 17, 2024 Category: Allergy & Immunology Authors: Yixian Guo Jinnan Chen Yu Huang Shouyu Ke Feng Xie Dan Li Bin Li Hong Lu Source Type: research
Increased infiltration of CD4 < sup > + < /sup > IL-17A < sup > + < /sup > FOXP3 < sup > + < /sup > T cells in Helicobacter pylori-induced gastritis
Eur J Immunol. 2024 Feb 17:e2350662. doi: 10.1002/eji.202350662. Online ahead of print.ABSTRACTHelicobacter pylori is one of the main predisposing factors for gastric cancer, causing chronic inflammation and proper glands atrophy in the gastric mucosa. Although H. pylori-induced inflammation is a key inducer of precancerous lesions in the gastric mucosa, it remains unclear which precise immune cell subsets are responsible for the progression of H. pylori-induced gastritis. Here, we observed an abundance of CD4+ IL-17A+ FOXP3+ T cells exhibiting a Th17-like phenotype within the microenvironment of H. pylori-induced gastriti...
Source: European Journal of Immunology - February 17, 2024 Category: Allergy & Immunology Authors: Yixian Guo Jinnan Chen Yu Huang Shouyu Ke Feng Xie Dan Li Bin Li Hong Lu Source Type: research
The breathtaking world of human respiratory in vitro models: Investigating lung diseases and infections in 3D models, organoids, and lung-on-chip
Eur J Immunol. 2024 Feb 15:e2250356. doi: 10.1002/eji.202250356. Online ahead of print.ABSTRACTThe COVID-19 pandemic illustrated an urgent need for sophisticated, human tissue models to rapidly test and develop effective treatment options against this newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Thus, in particular, the last 3 years faced an extensive boost in respiratory and pulmonary model development. Nowadays, 3D models, organoids and lung-on-chip, respiratory models in perfusion, or precision-cut lung slices are used to study complex research questions in human primary cells. These mode...
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Stefanie Dichtl Wilfried Posch Doris Wilflingseder Source Type: research
Extracellular vesicles: Messengers of allergic immune responses and novel therapeutic strategy
Eur J Immunol. 2024 Feb 15:e2350392. doi: 10.1002/eji.202350392. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are nanosized particles released by nearly every cell type across all kingdoms of life. As a result, EVs are ubiquitously present in various human body fluids. Composed of a lipid bilayer, EVs encapsulate proteins, nucleic acids, and metabolites, thus playing a crucial role in immunity, for example, by enabling intercellular communication. More recently, there has been increasing evidence that EVs can also act as key regulators of allergic immune responses. Their ability to facilitate cell-to-cell con...
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Christian M öbs Anna Lena Jung Source Type: research
Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages
We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.PMID:38361219 | DOI:10.1002/eji.202350515 (Source: European Journal of Immunology)
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Sara J Thygesen Sabrina S Burgener Prerna Mudai Mercedes Monteleone Dave Boucher Vitaliya Sagulenko Kate Schroder Katryn J Stacey Source Type: research
The breathtaking world of human respiratory in vitro models: Investigating lung diseases and infections in 3D models, organoids, and lung-on-chip
Eur J Immunol. 2024 Feb 15:e2250356. doi: 10.1002/eji.202250356. Online ahead of print.ABSTRACTThe COVID-19 pandemic illustrated an urgent need for sophisticated, human tissue models to rapidly test and develop effective treatment options against this newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Thus, in particular, the last 3 years faced an extensive boost in respiratory and pulmonary model development. Nowadays, 3D models, organoids and lung-on-chip, respiratory models in perfusion, or precision-cut lung slices are used to study complex research questions in human primary cells. These mode...
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Stefanie Dichtl Wilfried Posch Doris Wilflingseder Source Type: research
Extracellular vesicles: Messengers of allergic immune responses and novel therapeutic strategy
Eur J Immunol. 2024 Feb 15:e2350392. doi: 10.1002/eji.202350392. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are nanosized particles released by nearly every cell type across all kingdoms of life. As a result, EVs are ubiquitously present in various human body fluids. Composed of a lipid bilayer, EVs encapsulate proteins, nucleic acids, and metabolites, thus playing a crucial role in immunity, for example, by enabling intercellular communication. More recently, there has been increasing evidence that EVs can also act as key regulators of allergic immune responses. Their ability to facilitate cell-to-cell con...
Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Christian M öbs Anna Lena Jung Source Type: research
