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A kinetic characterization of the gill V(H < sup > + < /sup > )-ATPase from two hololimnetic populations of the Amazon River shrimp Macrobrachium amazonicum
Comp Biochem Physiol B Biochem Mol Biol. 2023 Jul 28:110880. doi: 10.1016/j.cbpb.2023.110880. Online ahead of print.ABSTRACTThis investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 ‰S) on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate that the V(H+)-ATPase c-subunit is distributed in the apical pillar cells of shrimps in fresh water but is absent after acclimation to 21 ‰S for 10 days. V(H+)-ATPase activity from the Tietê River popula...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - July 30, 2023 Category: Biochemistry Authors: Leonardo M Fabri Daniela P Gar çon Cintya M Moraes Marcelo R Pinto John C McNamara Francisco A Leone Source Type: research

Molecular and functional characterization of two granulocyte colony stimulating factors in goldfish (Carassius auratus L.)
Comp Biochem Physiol B Biochem Mol Biol. 2023 Jul 23;268:110879. doi: 10.1016/j.cbpb.2023.110879. Online ahead of print.ABSTRACTGranulocyte colony-stimulating factor (GCSF) is a member of the hematopoietic growth factor family that acts primarily on neutrophils and neutrophilic precursors to promote cell proliferation and differentiation. Although multiple GCSF genes have been found in teleosts, knowledge of their functions during fish hematopoietic development is still limited. Here, we report for the first time the molecular and functional characterization of two goldfish GCSFs (gfGCSF-a and gfGCSF-b). The open reading f...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - July 25, 2023 Category: Biochemistry Authors: Moussa Gouife Feiyang Wang Ziqi Ban Xinyuan Yue Jianhu Jiang Jiasong Xie Source Type: research

Molecular and functional characterization of two granulocyte colony stimulating factors in goldfish (Carassius auratus L.)
Comp Biochem Physiol B Biochem Mol Biol. 2023 Jul 23:110879. doi: 10.1016/j.cbpb.2023.110879. Online ahead of print.ABSTRACTGranulocyte colony-stimulating factor (GCSF) is a member of the hematopoietic growth factor family that acts primarily on neutrophils and neutrophilic precursors to promote cell proliferation and differentiation. Although multiple GCSF genes have been found in teleosts, knowledge of their functions during fish hematopoietic development is still limited. Here, we report for the first time the molecular and functional characterization of two goldfish GCSFs (gfGCSF-a and gfGCSF-b). The open reading frame...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - July 25, 2023 Category: Biochemistry Authors: Moussa Gouife Feiyang Wang Ziqi Ban Xinyuan Yue Jianhu Jiang Jiasong Xie Source Type: research

Diacylglycerol acyltransferase (DGAT) in Crangon crangon and Pandalus montagui (Decapoda, Caridea) - Implications for lipid storage capacities and life history traits
Comp Biochem Physiol B Biochem Mol Biol. 2023 Jul 20:110878. doi: 10.1016/j.cbpb.2023.110878. Online ahead of print.ABSTRACTLipids play essential roles in cell-structuring, cell-signaling, and as efficient metabolic energy stores. Lipid storage capacities determine life history traits of organisms and, thus, their ecological function. Among storage lipids, triacylglycerols (TAGs) are widespread in marine invertebrates. However, abilities to accumulate TAGs can vary even between closely related species, such as the caridean shrimps Crangon crangon and Pandalus montagui. The first species shows low TAG levels throughout the ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - July 22, 2023 Category: Biochemistry Authors: Marie Koch Jan Tebben Reinhard Saborowski Source Type: research

Potential role of miR-8159-x in heat stress response in rainbow trout (Oncorhynchus mykiss)
In conclusion, our findings suggest that miR-8159-x participates in the biological response to heat stress by targeting hsp90a1. These results contribute to a better understanding of the molecular mechanisms underlying heat stress in rainbow trout and provide valuable insights for future research.PMID:37356566 | DOI:10.1016/j.cbpb.2023.110877 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 25, 2023 Category: Biochemistry Authors: Guiyan Zhao Zhe Liu Jinqiang Quan Jun Sun Lanlan Li Junhao Lu Source Type: research