Circadian rhythm regulation in the sea cucumber Apostichopus japonicus: Insights into clock gene expression, photoperiod susceptibility, and neurohormone signaling
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 6:110930. doi: 10.1016/j.cbpb.2023.110930. Online ahead of print.ABSTRACTSea cucumber Apostichopus japonicus displays the typical circadian rhythms. This present study investigated the molecular regulation of clock genes, as well as monoamines and melatonin, in multiple tissues of A. japonicus, responding to the photoperiod. In order to determine their pivotal role in circadian rhythms, the crucial clock genes, namely AjClock, AjArnt1, AjCry1, and AjTimeless, were identified and a comprehensive analysis of their expressions across various tissues in adult A. japonicus was c...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 8, 2023 Category: Biochemistry Authors: Zhiqing Ye Ying Wei Guangbo Zhang Lifei Ge Chenqian Wu Yucheng Ren Jixiu Wang Xiuwen Xu Jingwen Yang Tianming Wang Source Type: research

Circadian rhythm regulation in the sea cucumber Apostichopus japonicus: Insights into clock gene expression, photoperiod susceptibility, and neurohormone signaling
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 6:110930. doi: 10.1016/j.cbpb.2023.110930. Online ahead of print.ABSTRACTSea cucumber Apostichopus japonicus displays the typical circadian rhythms. This present study investigated the molecular regulation of clock genes, as well as monoamines and melatonin, in multiple tissues of A. japonicus, responding to the photoperiod. In order to determine their pivotal role in circadian rhythms, the crucial clock genes, namely AjClock, AjArnt1, AjCry1, and AjTimeless, were identified and a comprehensive analysis of their expressions across various tissues in adult A. japonicus was c...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 8, 2023 Category: Biochemistry Authors: Zhiqing Ye Ying Wei Guangbo Zhang Lifei Ge Chenqian Wu Yucheng Ren Jixiu Wang Xiuwen Xu Jingwen Yang Tianming Wang Source Type: research

Oleic acid as modulator of oxidative stress in European sea bass (Dicentrarchus labrax) juveniles fed high dietary lipid levels
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 5:110929. doi: 10.1016/j.cbpb.2023.110929. Online ahead of print.ABSTRACTAlthough the benefits of oleic acid (OA) have been established in mammals, its effects on fish remain understudied. The aim of this study was to evaluate the antioxidant potential of OA in the liver, intestine, and muscle of European sea bass juveniles fed diets containing different lipid levels. For that purpose, six diets with 16 or 22% lipids and 0, 1, and 2% OA were formulated and triplicate groups of European sea bass juveniles (21.4 g) were fed with these experimental diets for 9 weeks. Increasin...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 7, 2023 Category: Biochemistry Authors: Nicole Martins Sara Moutinho Rui Magalh ães Pedro Pous ão-Ferreira Aires Oliva-Teles Helena Peres Carolina Castro Source Type: research

Oleic acid as modulator of oxidative stress in European sea bass (Dicentrarchus labrax) juveniles fed high dietary lipid levels
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 5:110929. doi: 10.1016/j.cbpb.2023.110929. Online ahead of print.ABSTRACTAlthough the benefits of oleic acid (OA) have been established in mammals, its effects on fish remain understudied. The aim of this study was to evaluate the antioxidant potential of OA in the liver, intestine, and muscle of European sea bass juveniles fed diets containing different lipid levels. For that purpose, six diets with 16 or 22% lipids and 0, 1, and 2% OA were formulated and triplicate groups of European sea bass juveniles (21.4 g) were fed with these experimental diets for 9 weeks. Increasin...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 7, 2023 Category: Biochemistry Authors: Nicole Martins Sara Moutinho Rui Magalh ães Pedro Pous ão-Ferreira Aires Oliva-Teles Helena Peres Carolina Castro Source Type: research

Identification of a cryptic functional apolipophorin-III domain within the Prominin-1 gene of Litopenaeus vannamei
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 1:110928. doi: 10.1016/j.cbpb.2023.110928. Online ahead of print.ABSTRACTThe Apolipophorin-III (apoLp-III) is reported as an essential protein element in lipids transport and incorporation in lepidopterans. Structurally, apoLp-III has an α-helix bundle structure composed of five α-helices. Interestingly, classic studies proposed a structural switch triggered by its interaction with lipids, where the α-helix bundle opens. Currently, the study of the apoLp-III has been limited to insects, with no homologs identified in other arthropods. By implementing a structure-based se...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 3, 2023 Category: Biochemistry Authors: Nallely Hoyos-Gonzalez Adrian Ochoa-Leyva Claudia G Benitez-Cardoza Luis G Brieba German Lukaszewicz Carlos H Trasvi ña-Arenas Rogerio R Sotelo-Mundo Source Type: research

Identification of a cryptic functional apolipophorin-III domain within the Prominin-1 gene of Litopenaeus vannamei
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 1:110928. doi: 10.1016/j.cbpb.2023.110928. Online ahead of print.ABSTRACTThe Apolipophorin-III (apoLp-III) is reported as an essential protein element in lipids transport and incorporation in lepidopterans. Structurally, apoLp-III has an α-helix bundle structure composed of five α-helices. Interestingly, classic studies proposed a structural switch triggered by its interaction with lipids, where the α-helix bundle opens. Currently, the study of the apoLp-III has been limited to insects, with no homologs identified in other arthropods. By implementing a structure-based se...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 3, 2023 Category: Biochemistry Authors: Nallely Hoyos-Gonzalez Adrian Ochoa-Leyva Claudia G Benitez-Cardoza Luis G Brieba German Lukaszewicz Carlos H Trasvi ña-Arenas Rogerio R Sotelo-Mundo Source Type: research

Identification of a cryptic functional apolipophorin-III domain within the Prominin-1 gene of Litopenaeus vannamei
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 1:110928. doi: 10.1016/j.cbpb.2023.110928. Online ahead of print.ABSTRACTThe Apolipophorin-III (apoLp-III) is reported as an essential protein element in lipids transport and incorporation in lepidopterans. Structurally, apoLp-III has an α-helix bundle structure composed of five α-helices. Interestingly, classic studies proposed a structural switch triggered by its interaction with lipids, where the α-helix bundle opens. Currently, the study of the apoLp-III has been limited to insects, with no homologs identified in other arthropods. By implementing a structure-based se...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 3, 2023 Category: Biochemistry Authors: Nallely Hoyos-Gonzalez Adrian Ochoa-Leyva Claudia G Benitez-Cardoza Luis G Brieba German Lukaszewicz Carlos H Trasvi ña-Arenas Rogerio R Sotelo-Mundo Source Type: research

Identification of a cryptic functional apolipophorin-III domain within the Prominin-1 gene of Litopenaeus vannamei
Comp Biochem Physiol B Biochem Mol Biol. 2023 Dec 1:110928. doi: 10.1016/j.cbpb.2023.110928. Online ahead of print.ABSTRACTThe Apolipophorin-III (apoLp-III) is reported as an essential protein element in lipids transport and incorporation in lepidopterans. Structurally, apoLp-III has an α-helix bundle structure composed of five α-helices. Interestingly, classic studies proposed a structural switch triggered by its interaction with lipids, where the α-helix bundle opens. Currently, the study of the apoLp-III has been limited to insects, with no homologs identified in other arthropods. By implementing a structure-based se...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 3, 2023 Category: Biochemistry Authors: Nallely Hoyos-Gonzalez Adrian Ochoa-Leyva Claudia G Benitez-Cardoza Luis G Brieba German Lukaszewicz Carlos H Trasvi ña-Arenas Rogerio R Sotelo-Mundo Source Type: research

Omega-3 PUFA and the fitness and cognition of the nematode Caenorhabditis elegans under different environmental conditions
Comp Biochem Physiol B Biochem Mol Biol. 2023 Nov 29:110925. doi: 10.1016/j.cbpb.2023.110925. Online ahead of print.ABSTRACTMany invertebrate species possess the metabolic ability to synthesize long-chain ω3 polyunsaturated fatty acids (PUFA) de novo. Due to their diverse effects on membrane architecture, neuroplasticity, growth and reproduction, PUFA have a high potential to positively influence the fitness of an organism. But how and when do these supposed advantages actually come into play? Other species, that are often closely related, pass natural selection without this special metabolic ability. The ω3-PUFA rich mo...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 1, 2023 Category: Biochemistry Authors: Ralph Menzel Xuchao Zhang Tamara Pietrucik Antonia Bathelt Liliane Ruess Source Type: research

Heat-shock protein 90 alleviates oxidative stress and reduces apoptosis in liver of Seriola aureovittata (yellowtail kingfish) under high-temperature stress
Comp Biochem Physiol B Biochem Mol Biol. 2023 Nov 29:110927. doi: 10.1016/j.cbpb.2023.110927. Online ahead of print.ABSTRACTHsp90s are molecular chaperones that enhance fish tolerance to high-temperature stress. However, the function of Hsp90s in Seriola aureovittata (yellowtail kingfish) under high-temperature stress remains largely unknown. Here, two Hsp90 isoforms were identified in S. aureovittata by bioinformatics analysis: SaHsp90α and SaHsp90β. The coding sequence of SaHsp90α was 2193-bp long and encoded a polypeptide of 730 amino acids; SaHsp90β was 2178-bp long and encoded a polypeptide of 725 amino acids. SaH...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 1, 2023 Category: Biochemistry Authors: Lin Wang Yan Jiang Lu Fang Changtao Guan Yongjiang Xu Source Type: research

Omega-3 PUFA and the fitness and cognition of the nematode Caenorhabditis elegans under different environmental conditions
Comp Biochem Physiol B Biochem Mol Biol. 2023 Nov 29:110925. doi: 10.1016/j.cbpb.2023.110925. Online ahead of print.ABSTRACTMany invertebrate species possess the metabolic ability to synthesize long-chain ω3 polyunsaturated fatty acids (PUFA) de novo. Due to their diverse effects on membrane architecture, neuroplasticity, growth and reproduction, PUFA have a high potential to positively influence the fitness of an organism. But how and when do these supposed advantages actually come into play? Other species, that are often closely related, pass natural selection without this special metabolic ability. The ω3-PUFA rich mo...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 1, 2023 Category: Biochemistry Authors: Ralph Menzel Xuchao Zhang Tamara Pietrucik Antonia Bathelt Liliane Ruess Source Type: research

Heat-shock protein 90 alleviates oxidative stress and reduces apoptosis in liver of Seriola aureovittata (yellowtail kingfish) under high-temperature stress
Comp Biochem Physiol B Biochem Mol Biol. 2023 Nov 29:110927. doi: 10.1016/j.cbpb.2023.110927. Online ahead of print.ABSTRACTHsp90s are molecular chaperones that enhance fish tolerance to high-temperature stress. However, the function of Hsp90s in Seriola aureovittata (yellowtail kingfish) under high-temperature stress remains largely unknown. Here, two Hsp90 isoforms were identified in S. aureovittata by bioinformatics analysis: SaHsp90α and SaHsp90β. The coding sequence of SaHsp90α was 2193-bp long and encoded a polypeptide of 730 amino acids; SaHsp90β was 2178-bp long and encoded a polypeptide of 725 amino acids. SaH...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - December 1, 2023 Category: Biochemistry Authors: Lin Wang Yan Jiang Lu Fang Changtao Guan Yongjiang Xu Source Type: research

Fatty acid translocase (FAT/CD36) in silver pomfret (Pampus argenteus): Molecular cloning and functional characterization
This study could contribute to a greater understanding of lipid metabolism and the development of effective strategies for nutrient requirements in fish.PMID:38036286 | DOI:10.1016/j.cbpb.2023.110926 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 30, 2023 Category: Biochemistry Authors: Peng Shi Ran Meng Jilin Xu Qian Zhang Guochao Ye Xiaojun Yan Kai Liao Source Type: research

Fatty acid translocase (FAT/CD36) in silver pomfret (Pampus argenteus): Molecular cloning and functional characterization
This study could contribute to a greater understanding of lipid metabolism and the development of effective strategies for nutrient requirements in fish.PMID:38036286 | DOI:10.1016/j.cbpb.2023.110926 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 30, 2023 Category: Biochemistry Authors: Peng Shi Ran Meng Jilin Xu Qian Zhang Guochao Ye Xiaojun Yan Kai Liao Source Type: research

A native strain of Bacillus subtilis increases lipid accumulation and modulates expression of genes related to digestion and amino acid metabolism in Litopenaeus vannamei
Comp Biochem Physiol B Biochem Mol Biol. 2023 Nov 22;270:110924. doi: 10.1016/j.cbpb.2023.110924. Online ahead of print.ABSTRACTIn the field of shrimp aquaculture, the utilization of probiotics represents a promising avenue, due to the well-documented benefits conferred by these microorganisms. In the current study, a Bacillus subtilis strain, referred to as strain E, was isolated from the gastrointestinal tract of the shrimp Litopenaeus vannamei and subsequently identified via molecular methods and phylogeny. The probiotic potential of strain E was characterized, and its application as a feed shrimp additive was evaluated...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 23, 2023 Category: Biochemistry Authors: Luiza Medeiros Laura Dall'Agno Jade Riet Bruna Nornberg Ra íza Azevedo Arthur Cardoso J éssica Lucinda Saldanha da Silva Oscarina Viana de Sousa Victor Torres Rosas Marcelo Borges Tesser Virg ínia F Pedrosa Luis A Romano Wilson Wasielesky Luis F Marins Source Type: research