Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Short communication: ROS production and mitochondrial membrane potential in hemocytes of marine bivalves, Mytilus galloprovincialis and Magallana gigas, under hypoosmotic stress
Comp Biochem Physiol B Biochem Mol Biol. 2023 Sep 6:110901. doi: 10.1016/j.cbpb.2023.110901. Online ahead of print.ABSTRACTBivalve mollusks that inhabit low-depth coastal and estuarine areas frequently experience osmotic stress that may be also associated with alterations of antioxidant enzyme activities and markers of oxidative stress. Mitochondria are a major source of reactive oxygen species (ROS) in eucaryotic cells. Overpoduction of ROS induces oxidative stress leading to a damage of intracellular compartments and cell death. In euryhaline bivalves, information concerning cellular ROS production upon osmotic stress an...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 8, 2023 Category: Biochemistry Authors: E S Kladchenko A A Tkachuk M S Podolskaya A Yu Andreyeva Source Type: research
Short communication: ROS production and mitochondrial membrane potential in hemocytes of marine bivalves, Mytilus galloprovincialis and Magallana gigas, under hypoosmotic stress
Comp Biochem Physiol B Biochem Mol Biol. 2023 Sep 6:110901. doi: 10.1016/j.cbpb.2023.110901. Online ahead of print.ABSTRACTBivalve mollusks that inhabit low-depth coastal and estuarine areas frequently experience osmotic stress that may be also associated with alterations of antioxidant enzyme activities and markers of oxidative stress. Mitochondria are a major source of reactive oxygen species (ROS) in eucaryotic cells. Overpoduction of ROS induces oxidative stress leading to a damage of intracellular compartments and cell death. In euryhaline bivalves, information concerning cellular ROS production upon osmotic stress an...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 8, 2023 Category: Biochemistry Authors: E S Kladchenko A A Tkachuk M S Podolskaya A Yu Andreyeva Source Type: research
Elevated temperature triggers increase in global DNA methylation, 5-methylcytosine expression levels, apoptosis and NOx levels in the gonads of Atlantic sea urchin
In this study, we used highly sensitive radioimmunoassay, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), in situ TUNEL assay, luminescence assay, and colorimetric assay techniques to investigate the impacts of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in gonads of Atlantic sea urchin (Arbacia punctulata, a commercially important species). Young adult sea urchins were exposed to 24, 28, and 32 °C for one week in a controlled laboratory setting. High temperatures (28 and 32 °C) markedly increased global DNA methylation (around 1.1-1.5-fold in testes and ~ 1.7-fo...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 6, 2023 Category: Biochemistry Authors: Md Saydur Rahman Mohammad Maruf Billah Victor Rangel Esmirna Cantu Source Type: research
Phosphofructokinase family genes in grass carp: Molecular identification and tissue-specific expression in response to glucose, insulin and glucagon
In this study, grass carp (Ctenopharyngodon idella) was used as animal model and the open reading frame (ORF) sequences of six PFK genetic isoforms of grass carp were cloned. Real-time PCR was used to detect its tissue distribution, and expression changes in oral glucose tolerance test (OGTT), insulin and glucagon injection experiments. The results showed that the ORF of pfkla, pfklb, pfkma, pfkmb, pfkpa and pfkpb genes was 2343, 2340, 2355, 2331, 2364 and 2349 bp in length, respectively. The results of tissue distribution showed that pfkla and pfklb, homologous to mammalian pfkl, exhibited low expression levels in the liv...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 6, 2023 Category: Biochemistry Authors: Yingxin Zhang Chaobin Qin Junli Wang Liping Yang Xiao Yan Shaoyang Zhi Guoxing Nie Source Type: research
Elevated temperature triggers increase in global DNA methylation, 5-methylcytosine expression levels, apoptosis and NOx levels in the gonads of Atlantic sea urchin
In this study, we used highly sensitive radioimmunoassay, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), in situ TUNEL assay, luminescence assay, and colorimetric assay techniques to investigate the impacts of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in gonads of Atlantic sea urchin (Arbacia punctulata, a commercially important species). Young adult sea urchins were exposed to 24, 28, and 32 °C for one week in a controlled laboratory setting. High temperatures (28 and 32 °C) markedly increased global DNA methylation (around 1.1-1.5-fold in testes and ~ 1.7-fo...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 6, 2023 Category: Biochemistry Authors: Md Saydur Rahman Mohammad Maruf Billah Victor Rangel Esmirna Cantu Source Type: research
Phosphofructokinase family genes in grass carp: Molecular identification and tissue-specific expression in response to glucose, insulin and glucagon
In this study, grass carp (Ctenopharyngodon idella) was used as animal model and the open reading frame (ORF) sequences of six PFK genetic isoforms of grass carp were cloned. Real-time PCR was used to detect its tissue distribution, and expression changes in oral glucose tolerance test (OGTT), insulin and glucagon injection experiments. The results showed that the ORF of pfkla, pfklb, pfkma, pfkmb, pfkpa and pfkpb genes was 2343, 2340, 2355, 2331, 2364 and 2349 bp in length, respectively. The results of tissue distribution showed that pfkla and pfklb, homologous to mammalian pfkl, exhibited low expression levels in the liv...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 6, 2023 Category: Biochemistry Authors: Yingxin Zhang Chaobin Qin Junli Wang Liping Yang Xiao Yan Shaoyang Zhi Guoxing Nie Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Short communication: Ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to Paenibacillus larvae
This study aimed to assess the oxidative stress levels in larvae exposed to three different strains of P. larvae through their diet by examining the ascorbyl radical (A) to ascorbate anion (AH¯) ratio. The results revealed that larvae inoculated with P. larvae exhibited a lower value of this index compared to uninoculated ones. Interestingly, the level of A remained constant, while the concentration of AH¯ increased. Said increase correlated with the virulence of the specific P. larvae strain used in the inoculation. These findings suggest a potential link between AH¯ molecules and a defense response in A. mellifera lar...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2023 Category: Biochemistry Authors: Mar ía de la Paz Moliné Matias E Aguirre Enzo Dom ínguez Paloma Moran Giardini Natalia J Fern ández Natalia Damiani M Sandra Churio Liesel B Gende Source Type: research
Estrogen regulates the transcription of guppy isotocin receptors
Comp Biochem Physiol B Biochem Mol Biol. 2023 Aug 21;269:110895. doi: 10.1016/j.cbpb.2023.110895. Online ahead of print.ABSTRACTEstrogen can regulate oxytocin receptor expression, which is mediated through estrogen receptors (ESRs) in mammals, initiating parturition. To further study the reproductive physiological process of ovoviviparous teleosts, guppies (Poecilia reticulata) were employed as the research model in the present study to identify the transcriptional regulation of ESRs on isotocin receptors (itrs). Since guppy embryos develop inside the ovary, in the present study, the levels of itrs in the ovarian stroma of...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 23, 2023 Category: Biochemistry Authors: Jianshuang Li Likang Lyu Haishen Wen Yun Li Xiaojie Wang Yijia Yao Xin Qi Source Type: research
