Did the use of minimum interventions for caries management change during the COVID-19 pandemic? A cross-sectional study
Aim: to evaluate changes in the frequency of use of minimum interventions (MI) techniques for caries management during the COVID-19 pandemic. Materials and Methods: a questionnaire was applied through the SurveyMonkey ™ platform to evaluate changes in the dentist’s frequency of use of non-invasive, micro-invasive, minimally invasive, and mixed interventions, non-aerosol, or aerosol productive, to manage dental caries before and during COVID-19 pandemic. Differences in the use of MI techniques were analyzed by Wilcoxon test and the effect size (ES) was calculated. An α = 0.05 was adopted. Results: a total of 781 dentis...
Source: Caries Research - January 9, 2023 Category: Dentistry Source Type: research

New Theory Illustrates the Formation of Enamel Cup-Shaped Lesions on Occlusal Surfaces
We propose a new theory for enamel cupping lesions formation. At early stages, naturally-formed cupping lesions showed increased porosity at two structural prismatic traits: the central cone extending into the enamel-dentine junction and the type-I Hunther-Schreger bands (HSB), suggesting them to be the main drivers for cupping lesion formation and development. In addition, these lesions were circumscribed by type-II HSBs, which present lower surface porosity and higher resistance to wear. This theory was verified in in vitro observations, where both the central cone and the type-I HSB of cuspal enamel showed higher suscep...
Source: Caries Research - December 29, 2022 Category: Dentistry Source Type: research

Accuracy of two visual criteria for the assessment of caries around restorations: a delayed-type cross-sectional study
The aim of this study was to compare the performance of two visual criteria used for the detection of caries around restorations in permanent teeth. In this delayed-type cross-sectional study, the study sample was randomly allocated to one of two visual criteria for the assessment of restorations, as follows: a) FDI criteria (International Dental Federation; considers marginal staining, marginal adaptation, and caries recurrence), and b) CARS (Caries Associated with Restorations or Sealants) criteria, defined by the International Caries Classification and Management System. A calibrated examiner assessed the restorations u...
Source: Caries Research - December 22, 2022 Category: Dentistry Source Type: research

Association between Carbonic Anhydrase VI (CA VI) gene copy number and dental caries experience
The current study examined the association between the carbonic anhydrase VI (CA VI) copy number variations (CNVs) and dental caries experience in adults. In total, 202 of 35-72 years old subjects participating in the Lithuanian National Oral Health Survey (LNOHS) agreed to provide saliva samples, thus their data were included in the current study. Information about sociodemographic, environmental, and behavioural determinants was acquired via the self-administered World Health Organisation (WHO) questionnaire. Fluoride levels in the drinking water were recorded based on information provided by water suppliers. Dental cari...
Source: Caries Research - December 20, 2022 Category: Dentistry Source Type: research

Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.
This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin. Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown ana...
Source: Caries Research - December 16, 2022 Category: Dentistry Source Type: research

Ability of a blue hemoglobin-based liquid as a novel technology to stain initial enamel demineralization – A proof-of-concept in-vitro study
During orthodontic treatment enamel demineralization can occur. Its early detection is the basis for efficient preventive measures to arrest or remineralize lesions. In the present study, the application of a novel blue hemoglobin-based liquid (BlueCheck) was evaluated as proof of concept for detection of on artificially demineralized smooth surfaces. 60 samples form extracted human posterior teeth were randomly assigned to four groups (15 per group). In 30 of these samples (groups A and B) superficial enamel was removed to create a ground surface. On the surface of other 30 samples (group C and D) orthodontic metal bracke...
Source: Caries Research - November 30, 2022 Category: Dentistry Source Type: research

Impacts of the COVID-19 pandemic exposure on child dental caries: Difference-in-differences analysis
The impact of the coronavirus disease 2019 (COVID-19) pandemic on dental caries is unknown. We investigated the effect of the pandemic on child dental caries in Japan by comparing the increase in dental caries from fourth to sixth grade between two cohorts, COVID-19 exposed cohort, and COVID-19 unexposed cohort, using difference-in-difference analysis. A longitudinal data that followed elementary school children in Adachi City, Tokyo, was analyzed. The analysis consisted of two cohorts: those who were in fourth grade in 2016 and sixth grade in 2018 (COVID-19 unexposed cohort, N = 399) and those who were in fourth grade in ...
Source: Caries Research - November 28, 2022 Category: Dentistry Source Type: research

Association between level of parental oral health literacy and the rational use of fluoride for children 0 to 4 years of age after instruction: intervention trial
An inadequate level of oral health literacy (OHL) can hinder the understanding of dental information, which can have a negative impact on health promotion actions, such as the rational use of fluoride. The aims of the present study were: 1) to look for association between parents/guardians ´ OHL and the amount of fluoride toothpaste used for children from zero to four years of age; 2) to compare the effect of different modes of educational interventions on the amount of fluoride toothpaste used for children from zero to four years of age; 3) to assess the ability of parents/guardians to choose a toothpaste with adequate f...
Source: Caries Research - November 16, 2022 Category: Dentistry Source Type: research

Changes in management preference of deep carious lesions and exposed pulps: questionnaire studies with a 10-year interval among dentists in Lithuania
We aimed to investigate changes in management preferences for deep carious lesions and pulps exposed during carious tissue removal that occurred during the last 10 years and identify associated dentists ’ background factors. The data was collected among dentists registered with the Lithuanian Dental Chamber at two time points using a similar questionnaire. In 2011, 400 randomly selected dentists received a questionnaire by mail, and 153 (38.3%) responded. In 2021, an electronic invitation to an o nline questionnaire was sent to all members of the Lithuanian Dental Chamber, and 213 (8.9%) dentists responded. The questionn...
Source: Caries Research - November 15, 2022 Category: Dentistry Source Type: research

Combined treatment with fluoride and antimicrobial peptide GH12 efficiently controls caries in vitro and in vivo
Combining fluoride and antimicrobial agents enhances regulation of acid and exopolysaccharide production by biofilms. The combination also weakens the acidogenic and aciduric bacteria that contribute to caries, achieving stronger caries-controlling effects with lower concentrations of fluoride. In previous studies, antimicrobial peptide GH12 has been shown to inhibit lactic acid and exopolysaccharide synthesis in various cariogenic biofilm models, and reduce the proportion of acidogenic bacteria and Keyes caries scores in a rat caries model. The current study aimed to elucidate the effect of a combination of low concentrat...
Source: Caries Research - November 15, 2022 Category: Dentistry Source Type: research

Acknowledgement of Financial Support
Caries Res 2022;56:382 (Source: Caries Research)
Source: Caries Research - November 3, 2022 Category: Dentistry Source Type: research

Diagnosis of interproximal caries lesions in bitewing radiographs using a deep convolutional neural network-based software.
The aim of this study was to evaluate the diagnostic reliability of a web-based artificial intelligence program for the detection of interproximal caries in bitewing radiographs. Three hundred bitewing radiographs of patients were subjected to the evaluation of a convolutional neural network. First, the images were visually evaluated by a previously trained and calibrated operator with radiodiagnosis experience. After, ground truth was established and was clinically validated. For enamel caries, clinical assessment included a combination of clinical-visual and radiography evaluations. For dentin caries, clinical validation...
Source: Caries Research - November 1, 2022 Category: Dentistry Source Type: research

Ultrastructure of the dentin pellicle and the impact of erosion
In conclusion, the dentin pellicle is neither acid-resistant nor able to effectively protect dentin from erosion. (Source: Caries Research)
Source: Caries Research - October 28, 2022 Category: Dentistry Source Type: research

Improved surface layer erosion of pit and fissure caries lesions in preparation for resin infiltration
The penetration of a low-viscosity resin (infiltrant) into caries lesions depends on the erosion of the relatively impermeable surface layer (SL) that covers the lesion body. The present study aimed to evaluate the effect of different abrasive pretreatments on erosion of the surface layer and on penetration of an infiltrant into occlusal caries lesions simultaneously. Sixty extracted human molars showing ICDAS-2 lesions were randomly allocated to 5 groups. A small area of each lesion was covered with resin before etching. Twelve lesions each were etched with either 15% HCl-gel (H120) or 37% H3PO4-gel (P120) for 120s. Furth...
Source: Caries Research - October 28, 2022 Category: Dentistry Source Type: research