Ability of a blue hemoglobin-based liquid as a novel technology to stain initial enamel demineralization – A proof-of-concept in-vitro study

During orthodontic treatment enamel demineralization can occur. Its early detection is the basis for efficient preventive measures to arrest or remineralize lesions. In the present study, the application of a novel blue hemoglobin-based liquid (BlueCheck) was evaluated as proof of concept for detection of on artificially demineralized smooth surfaces. 60 samples form extracted human posterior teeth were randomly assigned to four groups (15 per group). In 30 of these samples (groups A and B) superficial enamel was removed to create a ground surface. On the surface of other 30 samples (group C and D) orthodontic metal brackets were bonded. On each surface BC liquid was applied and rinsed with water after three minutes (baseline). All surfaces were checked by two independent observers for presence of blue areas. On each sample, one side was covered by nail varnish to protect this enamel part from demineralization. The samples were demineralized with lactic acid (pH 4.6) for 7 days (group A and C) and 14 days (group B and D), respectively. Mineral loss was determined using quantitative light induced fluorescence (QLF) after demineralization. BlueCheck dye was again applied on the samples and evaluated for presence of stained areas. Histological sections were prepared from randomly selected samples and lesion depth was measured. Kruskal-Wallis test was used for group comparison ( α=0.05). After demineralization, median ΔF value for all samples was: -8.25% indicating the presence...

Source: Caries Research - November 30, 2022 Category: Dentistry Source Type: research