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Correction to: Cyclooxygenase-2 Inhibitor Parecoxib Reduces LPS-Induced Activation of BV2 Microglia Cells
(Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - January 8, 2024 Category: Biology Source Type: research

Sex- and Age-Related Features of the Encephalometric Parameters of the Brain Hemispheres in Children of the Second Childhood Period
We performed a retrospective analysis of the encephalometric parameters of the brain hemispheres in children aged 8 years (30 boys and 30 girls) and 11 years (30 boys and 30 girls). The variability of the parameters of the brain hemispheres depending on age and sex was studied. In 8-year-old children, sex differences were revealed in a significant number of basic parameters: the length of the right and left hemispheres and the lengths of the left frontal lobe, right parietal lobe, right and left occipital lobes. In 11-year-old children, the sex differences were revealed only in the width of the hemispheres. Interhemispheri...
Source: Bulletin of Experimental Biology and Medicine - January 6, 2024 Category: Biology Source Type: research

Indicators of LPO and Antioxidant Protection in Rats During Long-Term Restriction of Motor Activity
This article presents a comprehensive study of the parameters of LPO (concentrations of malondialdehyde, diene conjugates, and intensity of chemiluminescence) and antioxidant defense (concentrations of α-tocopherol, ceruloplasmin, total antioxidant activity, antiradical activity, and activities of antioxidant enzymes — superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) in blood plasma and erythrocytes of white male rats during restriction of motor activity up to 28 days. The patterns of changes in oxidative balance in hypokinesia in the form of intensification of LPO processes against the...
Source: Bulletin of Experimental Biology and Medicine - January 5, 2024 Category: Biology Source Type: research

Pulsatile Features of Major Arteries in Rats during Hypotension Caused by Acute Blood Loss
The study examined the modes of pulsations of the femoral and carotid arteries of ratsin situ (n=14) during acute blood loss, which sequentially caused a deep BP drop, cardiac and respiration arrest, and termination of ECG signals. When BP dropped to 19 (13; 26) mm Hg, the mechanosensitivity of passively pulsing arteries determined by the ratio of the swing range of electroimpedance oscillations of arterial segment to pulse pressure increased by 3.2 (2 ; 4) times (p<0.05). During the decrease of BP to the threshold value of 13 (8; 15) mm Hg, the arterial pulsing mode changed from passive to intermediate and then to the ...
Source: Bulletin of Experimental Biology and Medicine - January 5, 2024 Category: Biology Source Type: research

Effects of Combined Exposure to Carbon Ions and Protons on the Pool of MCF-7 Breast Cancer Stem Cells In Vitro
We studied the effects of single and combined action of protons and carbon ions12C6+ on the pool of MCF-7 human breast cancer stem cells. Single irradiation with a beam of protons or carbon ions had no significant effects on the relative number of cancer stem cells (CSC). The effects of combined irradiation in a total equieffective dose of 4 Gy depended on the sequence of exposure to ionizing radiations: the relative number of CSC did not change after irradiation with carbon ions and then with protons, but increased in the case of the reverse sequence. The most favorable result,i.e. a decrease in the CSC pool, was observed...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

The Inhibitory Activity of Lactobacillus plantarum Supernatant against Enterobacteria, Campylobacter, and Tumor Cells
Cell-free supernatant ofLactobacillus plantarum exhibit a strong antimicrobial effect against a number of pathogenic enterobacteria (E. coli,Shigella flexneri,Salmonella typhimurium,Proteus mirabilis, andCampylobacter jejuni). The degree of growth inhibition in broth culture reached a high level for all tested bacteria. The highest rates were noted forP. mirabilis (by 13 times) and the lowest forS. flexneri (by 5 times) andC. jejuni (by 4.5 times). Significant antiproliferative effect of the supernatant on cells of tumor-derived epithelial cell lines was shown. The highest degree of inhibition (by 22 times) was observed fo...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Morphopathological Effect of Ferrihydrite Nanoparticles in an Experimental Model of Toxic Hemolytic Anemia
We studied the effect of biogenic ferrihydrite nanoparticles synthesized as a result of the culturing ofKlebsiella oxytoca on Wistar rats with experimental toxic hemolytic anemia. The pathology was simulated by single intraperitoneal injection of phenylhydrazine hydrochloride. On day 4, the functional parameters of erythrocytes in rats corresponded to the state of toxic hemolytic anemia. It is shown that ferrihydrite nanoparticles suspension has chronic toxicity and causes morphological changes in organs (mainly in the spleen), which are characterized by accumulation of nanoparticles. Administration of phenylhydrazine indu...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Effect of Adrenoreceptor Stimulation on Peptidergic Regulation of Cardiac Activity in Newborn Rats
We studied the effect of adrenoreceptor stimulation on the frequency of spontaneous activity and amplitude-time parameters of isometric contraction of the atrial myocardial strips from newborn rats, as well as the effect of Y receptor stimulation against the background of adrenoreceptor activation. After addition of Y1,5 receptor agonist [Leu31, Pro34] NPY (10—7 M), a tendency to a decrease in the effect of β1,2-adrenoreceptor agonist isoproterenol (10—5 M) on the frequency of spontaneous activity and atrial myocardial contractility was observed. The age-related features of the effect of NPY on the frequency of sponta...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

The State of the LPO —AOD System and the Relative Length of Telomeric Repeats in the Chromosomes of Blood Leukocytes in Obstructive Sleep Apnea Syndrome
We analyzed the relative length of telomeric repeats in peripheral blood leukocytes and indicators of oxidative stress in 32 men (mean age 51.2 ±3.1 years) with obstructive sleep apnea syndrome (OSAS). The control group consisted of volunteers without OSAS. The relative length of telomeres was determined in a DNA sample isolated from venous whole blood samples. The length of telomeric repeats of chromosomes was determined using quantitativ e real-time PCR (3 times for each DNA sample); albumin served as an internal control. The intensity of LPO processes and antioxidant protection was assessed by conventional spectrophoto...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Production and Study of Immunochemical Properties of Stabilized Env Trimer of Recombinant Form CRF63_02A6 of HIV-1
Stabilized trimers of the HIV-1 envelope glycoprotein Env are capable of inducing a potent and sustained broadly neutralizing antibody response in laboratory animals and therefore are attractive targets for anti-HIV vaccine development. In this work, a stable producer of the trimer Env recombinant form CRF63_02A6 of HIV-1 was derived from the CHO-K1 cell line. Using immunochemical assays, the trimers synthesized in CHO-K1 cells were shown to be recognized by both monoclonal broadly neutralizing antibodies and sera from HIV-positive patients. The resulting trimers of the recombinant form CRF63_02A6 of HIV-1 can be used both...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

TFP5, a Peptide Derived from Cdk5 Activator p35, Protects Pancreatic β Cells from Glucose Toxicity
We studied the effect of TFP5 on MIN6 cells (cultured mouse islet β cells) treated with different concentrations of glucose (5 or 25 mM). The results were verified in C57BL/6J mice (control;n=12) and db/db mice with type 2 diabetes mellitus (n=12). To synthesize TFP5, peptide p5 (a derivative of p35 protein, activator of cyclin-dependent kinase 5, Cdk5) was conjugated with a FITC tag at the N-terminus and an 11-amino acid TAT protein transduction domain at the C-terminus. TFP5 was employed to inhibit Cdk5 activity and then to evaluate its efficiency in treating experimental type 2 diabetes mellitus. TFP5 effectively inhib...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Uridine Administration Promotes Normalization of Heart Mitochondrial Function in Dystrophin-Deficient Mice and Decreases Tissue Fibrosis
The work shows the effect of the metabolic modulator uridine on the functioning and ultrastructure of heart mitochondria in dystrophin-deficientmdx mice. Intraperitoneal administration of uridine (30 mg/kg/day for 28 days) improved K+ transport and increased its content in the heart mitochondria ofmdx mice to the level of wild-type animals. This was accompanied by a significant decrease in the level of malondialdehyde and an increase in the number of mitochondria in the heart ofmdx mice. At the same time, uridine did not affect the hyperfunctionality of mitochondria inmdx mice, which manifested in an increase in the calciu...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

DNA Vaccine Encoding the Artificial T-Cell Polyepitope Immunogen of Tick-Borne Encephalitis Virus
A promising approach to the development of new means for preventing infection caused by tick-borne encephalitis virus can be DNA vaccines encoding polyepitope T-cell immunogens. A DNA vaccine pVAX-AG4-ub encoding an artificial polyepitope immunogen that includes cytotoxic and T-helper epitopes from the NS1, NS3, NS5, and E proteins of the tick-borne encephalitis virus has been obtained. The developed construct ensured the synthesis of the corresponding mRNAs in transfected eukaryotic cells. Immunization of mice with pVAX-AG4-ub induced the formation of a virus-specific T-cell response providing 50% protection from lethal i...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Anti-Inflammatory Effect of Technologically Processed Antibodies to the Molecules of the Major Histocompatibility Complex in a Model of Acute Inflammation In Vivo
The anti-inflammatory effect of technologically processed antibodies (TPA) to immune targets (MHC I and MHC II) was assessed in the carrageenan-induced rat paw edema model. The parameters “increase in edema” and “suppression of edema” significantly decreased (p<0.05) against the background of treatment with TPA and the reference drug indomethacin compared to the placebo group. The tested TPA produced an anti-inflammatory effect. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research

Functional Activity of Blood Neutrophils in Patients with Stable Course and Exacerbation of Chronic Obstructive Pulmonary Disease
We performed a comparative study of the parameters of chemiluminescence of blood neutrophils in patients with different severity of chronic obstructive pulmonary disease in its different periods. The maximum values of induced and spontaneous chemiluminescence were recorded at moderate severity of the disease during exacerbation. Low levels of chemiluminescence indicators were found in severe chronic obstructive pulmonary disease in the stable phase. The values of the induction period of the chemiluminescent response in patients with moderate chronic obstructive pulmonary disease were higher than in the control group. Corre...
Source: Bulletin of Experimental Biology and Medicine - December 13, 2023 Category: Biology Source Type: research