Particularities of Bone Regeneration in Rats after Implantation of Polycaprolactone Scaffold Mineralized with Vaterite with Adsorbed Tannic Acid
We studied the particularities of osteo- and angiogenesis in albino rats after implantation of polycaprolactone scaffolds mineralized with vaterite with adsorbed tannic acid in the femoral bone defect. It was found that the processes of angio- and osteogenesis in the bone tissue after scaffolds implantation depend on their biocompatibility. Implantation of non-biocompatible scaffolds was followed by activation of angio- and osteogenesis aimed at separation of these scaffold from surrounding tissues. Implantation of polycaprolactone/vaterite scaffolds containing tannic acid stimulated angio- and osteogenesis leading to vasc...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Collagen Peptides from Hyaline Cartilage for the Treatment and Prevention of Joint Diseases: Isolation and Characteristics
We studied the effects of Chymopsin and Caripazim on the proteolysis of collagen proteins from cattle tracheal hyaline cartilage. Homogenization of the cartilage under conditions of high pressure and temperature facilitated subsequent enzymatic hydrolysis: the degree of hydrolysis increased upon elevation of pressure from 40 to 80 mPa and temperature from 60 to 70 °C. Proteolysis with Chymopsin yielded collagen peptides with molecular weights from 900 to 7000 Da, while Caripazim processing yielded collagen peptides with lower molecular weights from 250 to 780 Da consisting of 2-8 amino acids, which could be easily abso...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Effect of Selank on Morphological Parameters of Rat Liver in Chronic Foot-Shock Stress
We studied the effects of Selank on morphological parameters of the liver in Wistar male rats subjected to chronic foot-shock stress. Selank was injected intraperitoneally in doses of 100, 300 and 1000 μg/kg 15 min before each stress session. Morphological and morphometrical analysis showed that chronic foot-shock stress induced hydropic degeneration of hepatocytes, an increase of the nucleus/cytoplasm ratio due to an increase in the area of nuclei and reduction of the cytoplasm area, the appeara nce of focal necroses, and lymphohistiocyte infiltration. Injection of Selank in all doses reduced the intensity of stress-in...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Effects of Cocarboxylase in Amikacin-Induced Ototoxicity in Immature Animals
Possible otoprotective properties of cocarboxylase were studied on the model of amikacin-induced ototoxicity in immature rabbits. Auditory function was evaluated by the short-latency auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) tests. Administration of cocarboxylase after modeling of amikacin-induced ototoxicity resulted in a decrease in the ABR peak I threshold and prevented damage to the outer hair cells. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Morphological Changes in the Retina Under Conditions of Experimental In Vivo Regional Ischemia/Reperfusion
Two models of retinal ischemia/reperfusion were developed in an experiment on rats and structural changes in eye tissues in the early and late postischemic periods were studied. Ischemia/reperfusion was modeled by elevation of intraocular pressure to 110 mm Hg for 30 min with air injection into the anterior chamber of the eye with a special device or subconjunctival administration of 0.2 ml 4 ×10–6 M endothelin-1. Morphological studies of the retina of enucleated eyes were performed in 3, 7, and 30 days after ischemia/reperfusion. In 3 days, signs of retinal ischemia were seen (retinal edema and ganglion cell d...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Analysis of the Action of Quaternized Chitosans with Different Molecular Weight on Anticoagulant Activity of Heparins In Vitro
Quaternized derivatives of chitosan with a substitution degree of 85-98% (highly substituted) synthesized from chitosans with a molecular weight of 5, 10, 20 kDa, with a degree of deacetylation of 89-98%, and the code numbers of QChit 5, QChit 10, QChit 20, respectively, completely neutralize antithrombin activity of unfractionated heparin and partially neutralize aXa activity of low-molecular-weight heparin (clexane), similar to protamine sulfate. The advantages of QChit 5 and QChit 10 over QChit 20 and protamine sulphate are the follows: the effect is achieved at lower concentrations and in greater concentration range; t...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Characteristics of Artificial Immunogens Containing Peptide Mimotopes of HIV-1 Epitopes Recognized by Monoclonal Antibodies 2F5 and 2G12
The paper describes construction of TBI-based recombinant proteins TBI-2F5 and TBI-2G12 that contain peptide mimotopes of HIV-1 epitopes recognized by broadly neutralizing antibodies 2F5 and 2G12, respectively. The capacity of the immunogens to induce neutralizing antibodies was evaluated. The sera of BALB/c mice immunized with recombinant proteins TBI, TBI-2F5, and TBI-2G12 neutralized HIV-1env-pseudoviruses. Moreover, pooled serum from mice immunized with TBI-2F5 and TBI-2G12 neutralizedenv-pseudoviruses of HIV-1 subtype B more effectively than individual sera. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

β-Amyloid Peptide Antagonizes the Effect of Protons on Taurine-Induced Chloride Current in Rat Hippocampal Pyramidal Neurons
Taurine is an important endogenous agonist of glycine receptors (GlyR). Using the patchclamp technique, we measured chloride current induced by a short (600 msec) application of taurine (ITau) on isolated rat pyramidal neurons. pH of taurine solution in the applicator pipette was neutral (7.4) or acidic (7.0-5.0). Application of protons to a neuron causes a dosedependent decrease in the peak amplitude and acceleration of ITau desensitization. Addition of 100 nM β-amyloid peptide (Aβ) to the perfusate caused acceleration of ITau desensitization. The effects of A β and H+ on the rate of ITau desensitization we...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Contractile Activity of Lymph Nodes in Young, Middle-Aged, and Old Rats
We studied age-associated changes in contractile activity of isolated cervical and mesenteric lymph nodes in rats. In young and middle-aged rats, no significant changes in contractile activity of lymph nodes were revealed. In old rats, the amplitude of spontaneous contractions of lymph nodes was lower and their rate was higher than in young and middle-aged animals; the sensitivity of lymph nodes to vasoactive agents was reduced and the threshold of the response was higher in old rats. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Effects of Quercetin on Expression of Genes of Carbohydrate and Lipid Metabolism Enzymes in the Liver of Rats Receiving High-Fructose Ration
We studied the expression of genes encoding enzymes of carbohydrate and lipid metabolism ketohexokinase (Khk), glucokinase (Gck), pyruvate kinase (Pklr), acetyl-Co-carboxylase (Acaca), fatty acid synthase (Fasn), stearoyl-CoA desaturase (Scd), and their transcription regulators ChREBP (Mlxipl), SREBP-1c (Srebf1), and PPAR α (Ppara) in rat liver. Control group rats received a semisynthetic ration over 20 weeks. Experimental group 1 received a semisynthetic ration and 20% fructose solution instead of drinking water. Experimental group 2 rats received a semisynthetic ration with quercetin (0.1% fodder weight) and 20% fr...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Contrast Agents Based on Iron Oxide Nanoparticles for Clinical Magnetic Resonance Imaging
Magnetic resonance imaging (MRI) is one of the most perspective methods of noninvasive visualization in medicine, and use of contrast agents significantly its potentialities extends. Iron oxide nanoparticles are promising contrast agents, but in fact all the data on their efficiency were obtained in high-field tomographs for experimental animals. We studied the possibility of using magnetic nanoparticles for MRI visualization of rat brain glioblastoma at the most common clinical field 1.5 T The data indicate the efficiency of iron oxide magnetic nanoparticles as contrast agents for 1.5 T MR tomographs. (Source: Bulletin of...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

The Structure of NO-Mediated Dilatation of Pulmonary Arteries Depends on NO Availability
NO-mediated vasodilatation can be realized via two pathways: dependent and independent on soluble guanylate cyclase; the latter is implemented through NO interaction with ionic channels. We evaluated the contribution of these pathways into NO-induced relaxation of isolated pulmonary arteries in rats. In pulmonary arteries, in contrast to systemic vessels, soluble guanylate cyclase-independent mechanisms is more important, because it mediates relaxation in response to low concentrations of NO donor. The role of soluble guanylate cyclase-dependent mechanisms in the mechanisms of vasodilatation increases with increasing NO do...
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Morphological Changes in the Thymus of Newborn Rats Exposed to Endocrine Disruptor Dichlorodiphenyltrichloroethane (DDT) during the Prenatal Period
The structure of the thymus in newborn rats exposedin utero to endocrine disruptor DDT was studied. We found that exposure to low doses of DDT during pregnancy does not disturb thymus formation in the offspring, but leads to changes in its structure than manifested as slower lobulation, wider epithelium-free areas in the cortex, and lower number of thymic corpuscles in the medulla. The results attest to sensitivity of the reticuloepithelial stroma of the thymus to the effects of endocrine disruptor. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 26, 2019 Category: Biology Source Type: research

Relationship between Cooling Medium and Hepatocyte Ploidometric Parameters in Albino Rats
Relationship between the method of hypothermic exposure and ploidometric characteristics of hepatocyte nuclei of rats were studied immediately after hypothermic exposure and during the posthypothermic period. The following cooling modes were used: single submersion into 5 °C water (until rectal temperature drop to 20-25°C; deep hypothermia) and single exposure at air temperature of -25°C (until rectal temperature drop to 30°C; moderate hypothermia). A manifest destructive effect of deep hypothermia on rat liver was detected: the exposure caused manifest alteratio n of hepatocytes (alteration index increased...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Peculiarities of Intracellular Signal Transduction in the Regulation of Functions of Mesenchymal, Neural, and Hematopoietic Progenitor Cells
The role of NF- κB, cAMP/PKA, JAKs/STAT3, ERK1/2, p38, JNK, and p53 signaling pathways in the realization of growth potential of mesenchymal, neural, erythroid, and granulomonocytic progenitor cells were examinedin vitro. Using selective blockers of signaling molecules, we revealed some principal distinctions of their involvement in determination of proliferation-differentiation status of the progenitor cells of different functional classes. The most salient peculiarities were observed in the roles of cAMP/PKA, JNK, and JAKs/STAT3 signaling pathways in the control of functions of various types of the regeneration-com...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Effect of Linagliptin on the Ratio of Apoptosis Regulators in the Model of Non-Alcoholic Fatty Liver Disease in db / db Mice
We studied the effects of dipeptidyl peptidase 4 (DPP4) inhibitor linagliptin on the expression of apoptosis regulator proteins Bcl-2 and Bad in the liver ofdb/db mice with genetically determined obesity and type 2 diabetes mellitus. The mice received daily linagliptin or saline (placebo) by gavage from week 10 to week 18 of life. In the liver of non-treated mice, the area positively stained for Bad was greater than the area of Bcl-2 expression, which created the conditions for apoptosis activation in liver at this age. Administration of linagliptin decreased Bad stained area and increased Bcl-2 stained area in the liver c...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Changes in Activities of Somatovisceral Systems in Newborn Rats under Conditions of Nicotinic Cholinoreceptor Blockage and Activation of Cholinoreactive Structures
Relationship between the residual effects of disorders in the cholinergic system and the activities of the cardiac, respiratory, and somatomotor systems of 1- and 16-day-old rats were studied. Experiments were carried out on intact conscious rats before and after drug injection (nicotinic cholinoreceptor blocker benzohexonium). In order to increase the level of cholinoreactive structure activation, acetylcholinesterase inhibition by eserine was carried out. Injection of benzohexonium caused rarefaction of HR, respiration rate, and a decrease of motor activity parameters in rats of both age groups. Injection of eserine afte...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Role of MAPK ERK1/2 and p38 in the Realization of Growth Potential of Various Types of Regeneration-Competent Cells in Mouse Neural Tissue during Ethanol-Induced Neurodegeneration In Vitro
Ethanol-induced neurodegeneration was modeledin vitro to study the roles of ERK1/2 and p38 in realization of the growth potential of neural progenitor cells and secretion of neurotrophic growth factors by glial elements. Addition of the neurotoxic dose of C2H5OH (65 mM) to the culture medium abolished the effects of specific ERK1/2 and p38 inhibitors on the formation of colonies (neurospheres) and proliferative activity of neural CFU in cultured cells derived from paraventricular region of the mouse brain. The study established that these protein kinases are not implicated in ethanol-induced stimulation of the formation of...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

N-Terminal Fragment of Brain-Type Natriuretic Peptide (NT-proBNP) as a Prognostic Marker in Patients with Newly Diagnosed Multiple Myeloma Complicated by Dialysis-Dependent Renal Failure
Prognostic value of N-terminal fragment of the prohormone brain-type natriuretic peptide (NT-proBNP) was analyzed in patients with multiple myeloma complicated by dialysisdependent renal failure. The prospective study included 20 patients with newly diagnosed multiple myeloma. The concentrations of NT-proBNP were measured before antimyeloma chemotherapy. The median age of the patients was 67 (63-76) years. The median glomerular filtration rate was 4 (4, 5) ml/min/1.73 m2. For overall survival, the area under ROC curve was 0.75 and the cut-off point was 7000 pg/ml. At median follow-up of 17.3 months, the overall survival wa...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Water Immersion as a Model of Hypogravity
We studied the effects of hypogravity modeled by water immersion on cognitive functions and physiological parameters of monkeys. Cognitive capacities of monkeys were evaluated using computer-controlled joystick task with food reward in case of target hit. Water immersion (3 days for 3 h) affected in cognitive functions, body temperature, and blood parameters. The intensity of changes depended on the type of monkey behavior. In animals with non-aggressive behavior, the number of target hits did not decrease after water immersion, and even slightly increased. On the contrary, aggressive monkeys showed poorer test performance...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Structural Analysis of Peripheral Organs of the Immune System in the Assessment of Grippol Vaccine Safety
The safety of Grippol vaccine was assessed by macro- and microscopic methods in the experiment. The morphological features of group lymphoid nodules of the small intestine were studied on days 4, 7, 14 and 30 after vaccination. The structural morphological dynamics of lymphoid tissue under these conditions is shown. Because of the use of the vaccine, the lymphocytopoietic processes in the body are activated, which indicates the formation of an immune response. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

State of Colon Microbiota in Rats during Chronic Restraint Stress and Selank Treatment
We studied the effects of Selank on intestinal microbiota in Wistar male rats subjected to chronic restraint stress. Selank was injected intraperitoneally in doses of 80, 250 and 750 μg/kg 15 min before stress exposure. Chronic restraint stress led to a decrease in the content of obligate microflora, while the content of opportunistic microorganisms increased. Selank restored intestinal microbiota presumably via central (neurotropic) and peripheral (immunotropic) mechanisms. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Effect of NO Synthesis Blockade on the Hydrouretic Function and Extracellular Matrix of the Kidneys in Rats with Different Blood Vasopressin Levels
We studied the effect of NO synthesis blockade on the hydrouretic function and the state of renal interstitial matrix in WAG rats with normal blood vasopressin level and Brattleboro rats lacking endogenous vasopressin. In WAG rats, impaired osmotic concentration accompanied by an increase in the content of histochemically detectable glycosaminoglycans in the inner medullary interstitium was observed under conditions of NO synthesis blockade. In Brattleboro rats, NO synthesis inhibition, on the contrary, induced an antidiuretic response without significant changes in the renal extracellular matrix. These findings attested t...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Effects of Helium Cold Plasma on Erythrocyte Metabolism
The effects of helium cold plasma on some parameters of metabolism of rat erythrocytes were studied after a course exposure (10 daily procedures, 1 min each). The intensity of free-radical processes in erythrocyte membranes, malondialdehyde concentration, and aldehyde dehydrogenase activity were analyzed. The exposure to helium cold plasma led to a significant increase in malondialdehyde level mostly associated with inhibition of its utilization. These changes were not related to activation of malondialdehyde synthesis in LPO reactions. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Age-Dependent Effects of NO on Rhythmic Activity of Postganglionic Sympathetic Fibers
We studied the effects of exogenous NO donor (sodium nitroprusside) and NO synthesis blocker (100 μM L-NAME) on baseline electrical activity of postganglionic fibers in the sympathetic superior cervical in rats during postnatal ontogeny. Starting the age of 20 days, sodium nitroprusside increased the mean discharge amplitude and the spectrum power in the respiratory (0.7-1.5 Hz) and cardiac (4- 7 Hz) frequency bands. In contrast, application of L-NAME for 1 h decreased the spectrum power in these bands. Both agents produced no significant effect on the rhythmic sympathetic discharges at frequencies>8 Hz. (Source: Bul...
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

The Level of Neuregulin-1 after Traumatic Brain Injury and Formation of Post-Traumatic Epilepsy
We evaluated the serum level of neuregulin-1 in humans with traumatic brain injury. The highest levels of neuregulin-1 were revealed in patients with developing post-traumatic epilepsy and the lowest concentrations of this peptide were found in healthy controls. The patients with traumatic brain injury not aggravated by the development of post-traumatic epilepsy had intermediate levels of neuregulin-1. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 24, 2019 Category: Biology Source Type: research

Effect of Interleukin-10 on Localization of AMPA Receptors in Synapses during Long-Term Posttetanic Potentiation in Cultured Hippocampal Slices
The effect of the anti-inflammatory cytokine IL-10 on the ultrastructural distribution of AMPA receptor GluR1 subunit in CA1 field of cultured hippocampal slices was studied by using immunohistochemical technique. It was found that long-term posttetanic potentiation increased the content of GluR1 in the postsynaptic density of the axo-spinous synapse. Addition of IL-10 in concentrations of 1 and 10 ng/ml to the medium facilitated long-term posttetanic potentiation thereby changing the distribution of GluR1 in the spine: the number of receptors increased in the cytoplasm and decreased in the postsynaptic density. It is assu...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Cell Sheets of Mesenchymal Stromal Cells Effectively Stimulate Healing of Deep Soft Tissue Defects
We used rat model of splinted defect of the skin and soft tissues to compare the efficiency f mesenchymal stromal cells applied in sheets or in suspension for the treatment of these injuries. Transplantation of mesenchymal stromal cells significantly accelerated wound healing in comparison with the control. In the group treated by application of mesenchymal stromal cell sheets, the defect was closed by day 28, in the group treated with cell suspension by day 35, and in the control group after 49 days. According to histological analysis of the tissue samples, the formation of the granulation tissue and fibrosis occurred ear...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment
Intravital microscopy is widely used forin vivo studies of the mechanisms of carcinogenesis and response to antitumor therapy. For visualization of tumor cellsin vivo, cell lines expressing fluorescent proteins are needed. Expression of exogenous proteins can affect cell growth rate and their tumorigenic potential. Therefore, comprehensive analysis of the morphofunctional properties of transduced cells is required for creating appropriate models of tumor microenvironment. In the present study, six lines of mouse tumor cells expressing green and red fluorescent proteins were derived. Analysis of cells morphology, growth kin...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Interaction of NK Cells, Trophoblast, and Endothelial Cells during Angiogenesis
We studied changes in angiogenesis during contact interaction of natural killer cells and endothelial cells in the presence of secretory products of trophoblast cells activated by various cytokines. Activated trophoblast regulates angiogenesis by producing soluble factors that affect endothelial cells either directly or indirectly through activation of proangiogenic activity of natural killer cells. A stimulating effect of the trophoblast supernatants activated by IL-1 β and an inhibitory effect of trophoblast supernatants activated by IL-6 and TGFβ for the formation of tube-like structures by endothelial cells w...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Effect of Low-Level Laser Irradiation on Proliferative Activity of Wharton ’s Jelly Mesenchymal Stromal Cells
We studied the effect of low-level laser irradiation on proliferative activity of cultured human Wharton ’s jelly mesenchymal stromal sells. Cells were irradiated with a solid-state laser emitting at 650 nm; irradiation doses were 0.04, 0.4, or 4 J/cm2. Laser irradiation was performed once at the start of the cell proliferation experiment or daily throughout the experiment. Cells were cultured for 7 days. The number of viable cells was assessed using the MTT test. An increase in cell proliferative activity was detected after daily laser irradiations; the maximum stimulating effect was achieved at a dose of 0.04 J/cm2...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin
We compared phagocytic activity of macrophages of monocyte origin and Kupffer cells under the influence of M1 and M2 inducers and without activation. Cultures of monocyte-derived macrophages and Kupffer cells were characterized by intensive expression of CD68 that was not affected by activation factors. At the same time, these cultures demonstrated different dynamics of phagocytic activity. Monocyte-derived macrophages initially had more pronounced absorption capacity that gradually increased during the experiment. Kupffer cells were characterized by abrupt fluctuations of phagocytic activity: sharp growth and rapid satura...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Study of the Osteoindictive Properties of Protein-Modified Polylactide Scaffolds
Bone marrow mesenchymal stromal cells are multipotent and can differentiate into cells of various tissues, which determines their high importance for clinical application. We performed anin vitro study of the osteogenic potential of mesenchymal stromal cells cultured on intact polylactide scaffolds or scaffolds modified with collagen I or fibrin. Scanning electron microscopy showed that the cells formed osteogenic nodules or osteogenic nodules on both intact and fibrin-modified polylactide scaffolds. Spectrophotometric detection of alkaline phosphatase activity on days 7 and 11 showed that mesenchymal stromal cell grown on...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Effect of Storage Conditions on the Integrity of Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles
We studied the effect of storage conditions on the safety of microvesicles produced by human multipotent umbilical cord mesenchymal stromal cells into the conditioned medium. It was found that microvesicles can be stored without serious degradation for up to 1 week at 4 °С, but were almost completely destroyed during freezing and thawing cycles irrespective of the storage temperatures (-20°С, -70°С, or -196°С). Similar results were obtained for lyophilized medium conditioned by human multipotent umbilical cord mesenchymal stromal cells. Addition of a cryopr otectant (5-10% DMSO) followed by freezing and...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Bone Marrow Multipotent Mesenchymal Stromal Cells in Patients with Diffuse Large B-Cell Lymphoma
In diffuse large B-cell lymphoma, bone marrow involvement is rarely diagnosed. We compared the properties of bone marrow stromal progenitor cells and the concentration of fibroblast CFU in patients with diffuse large B-cell lymphoma without bone marrow involvement and in healthy donors. It was found that the properties of multipotent mesenchymal stromal cells in patients in the debut of the disease differed considerably from those in healthy donors. In particular, the total cell production in patients was significantly higher than in donors. In multipotent mesenchymal stromal cells of patients, some cell parameters were ch...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Skin Fibroblasts as the Object for Clinical Diagnosis of Parkinson ’s Disease in Persons of Different Ages
We compared the expression of A β42 peptide, τ-protein, and α-synuclein in the substantia nigra and skin fibroblasts of elderly and senile patients with Parkinson’s disease and subjects without neuropathology. Expression of markers in the studied tissues was assessed by immunohistochemical and immunocytochemical methods. The expression of Aβ42 peptide, τ-protein, and α-synuclein in the substantia nigra of elderly and senile patients with Parkinson’s disease was higher by 11-31 times than in subjects without neuropathology. In skin fibroblasts of patients with Parkinson’s dise...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Co-Transplantation of Pancreatic Islet Cells and Mesenchymal Bone Marrow Precursors on Titanium Nickelide Scaffolds in Alloxan-Induced Diabetes Mellitus
We studied therapeutic activity of co-transplantation of allogeneic pancreatic islet cells and mesenchymal bone marrow progenitors on TiNi scaffolds in Wistar rats with experimental alloxan-induced diabetes mellitus. In preliminary experiments with co-culturing of cells in different proportions followed by their transplantation on tissue-engineered constructs, the optimum ratio of these cells was determined — 3:1. Regeneration was assessed by biochemical methods by the blood levels of glucose and glycosylated hemoglobin on days 15, 30, and 5. In the group with combined cell transplantation on TiNi scaffold, normaliza...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Mesenchymal Stem Cells from Mouse Adipose Tissue Stimulate Tumor Growth
We studied the effect of mesenchymal stem cells from the bone marrow and adipose tissue on the growth rate of melanoma B16 and mammary adenocarcinoma Ca755 tumors after their co-administration with tumor cells to syngeneic mice. Stimulation of tumor growth and formation of melanoma metastases in the lungs was found under the influence of adipose tissue-derived, but not bone marrow-derived stem cells. At delayed terms after irradiation in sublethal doses, the adipose tissue-derived mesenchymal stem cells also stimulated the tumor growth. Stimulation of the tumor growth by adipose tissue-derived mesenchymal stem cells was ca...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Isolation of IgG Associated with Human Placenta
We optimized the method of isolation of antibodies from placental tissue of a conventionally healthy patient. Four protocols of antibody isolation were evaluated and a protocol with tissue grinding (without homogenization) and successive elution of the antibodies with acidic and alkaline buffers was recommended for use. The repertoire of the isolated antibodies was characterized using a glycan array. Partial coincidence of the specificity of the isolated antibodies with antibodies in the peripheral blood was demonstrated, which indicates their possible association with carbohydrate antigens in the placenta. Identification ...
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Relaxation Properties of Contrast Media for MRI Based on Iron Oxide Nanoparticles in Different Magnetic Fields
We studied dependences of T2 relaxation time on magnetic field and concentration of nanoparticles. It was found that nanocontrast media are effective under the influence of the magnetic fields in the range 0.3-7 T. Data of electron paramagnetic resonance confirm the assumption on aggregation of nanoparticles not coated with proteins in high magnetic fields. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Correction to: Abnormal Membrane Localization of α2 Isoform of Na,K-ATPase in m. soleus of Dysferlin-Deficient Mice
The third author ’s name should read:V. V. Matchkov. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - June 10, 2019 Category: Biology Source Type: research

Oncolytic Activity of the Vaccine Strain of Type 3 Poliovirus on the Model of Rat Glioma C6 Cells
Rat glioma cell line C6 expressing human poliovirus receptor (PVR) and susceptible to polioviruses (C6-PVR-BFP) was used to produce a clone with knockout of IFN α/β (Ifnar1) receptor subunit 1 gene (Ifnar1). The sensitivity of C6-PVR-BFP cells to the vaccine strain of poliovirus type 3 (PV3) depended on the signaling pathways of the cell response to type 1 IFN. Using the model of subcutaneous tumor xenografts, we demonstrated oncolytic activity of PV3 against C6-PVR-BFP cells that depended on the expression of PVR and increased considerably upon disturbances in IFN response pathways. (Source: Bulletin of Experim...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Oxidative Modification of Redox Proteins: Role in the Regulation of HBL-100 Cell Proliferation
HBL-100 breast epithelial cells were cultured with a blocker (N-ethylmaleimide) and protector (1,4-dithioerythritol) of SH groups. The study assessed changes in redox potential of glutathione and thioredoxin systems, intensity of oxidative modification of proteins, ROS production, and cell proliferation. The roles of thioredoxin system and protein oxidative modification in HBL-100 cell proliferation under redox status modulation were established. The role of carbonylated thioredoxin in arrest of the cell cycle in S-phase was demonstrated, which could be used for targeted therapy of the diseases accompanied by oxidative str...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Hypermethylated Genes of MicroRNA in Ovarian Carcinoma: Metastasis Prediction Marker Systems
We identified a group of miRNA genes whose methylation is associated with ovarian cancer metastasis. Based on these data, new markers and the systems of markers predicting tumor dissemination were selected. Using methylation-specific PCR and a representative set of 54 ovarian cancer samples, we identified 10 microRNA genes (MIR-124a-2,MIR-127,MIR-125b-1,MIR-129-2,MIR-137,MIR-193a,MIR-203a,MIR-34b/c,MIR-130b, andMIR-1258) whose methylation is associated with tumor metastasis. The greatest association was established for 4 genes:MIR-137,MIR-193a,MIR-34b/c, andMIR-130b (p
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Effect of Estriol, Chorionic Gonadotropin, and Oncostatin M on the Expression of Recombinase RAG-1 in Regulatory T Lymphocyte Subpopulations
We studied the effect of estriol, chorionic gonadotropin, oncostatin M, and hormone-cytokine combinations on the expression of recombinase RAG-1 in T-regulatory (Treg) and T helper 17 (Th17) lymphocytes. It was found that estriol in a concentration corresponding to the first trimester of pregnancy increased the level of Treg (CD4+FoxP3+) cells and suppressed the formation of Th17 (CD4+RORC+) lymphocytes. This effect was nor observed after individual administration of chorionic gonadotropin and oncostatin M, but some combinations of the studied hormones with oncostatin M increased the percentage of CD4+FOXP3+ cells. In the ...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Effect of Endogenous Neuropeptide Cycloprolylglycine on GABA A Receptors in Cerebellar Purkinje Cells
Voltage clamp and concentration-jump methods were employed to examine the effects of endogenous neuropeptide cycloprolylglycine on GABA-activated ionic currents in isolated cerebellar Purkinje cells. In the concentration range of 0.1-10.0 μM, short-term (600 msec) external application of cycloprolylglycine against the background of GABA-evoked current produced no effect on its amplitude. In contrast, application of 1 μM cycloprolylglycine increased current up to 177±15% control level. The development of potentiating effect and ret urn to the control level of ionic current were slow, which was indicative of pos...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Cytokine Content in the Hypothalamus and Hippocampus of C57Bl/6J Mice with Depressive-Like Behavior
The content of pro- and anti-inflammatory cytokines in the hypothalamus, hippocampus, and blood serum of C57Bl/6J mice with depressive-like behavior induced by 20-day social stress was analyzed in 4 h after immune stimulation with LPS (250 μg/kg). These animals are characterized by a tendency to an increase in the blood content of IL-6 and a decrease in the level of IL-10. Changes in cytokine content in the brain of mice with depressive-like state developed under these conditions were observed only in the hippocampus: the levels of I L-1β, IL-6, TNFα, and IL-10 increased and the content of IFNγ decrease...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Effect of Non-Thermal Plasma on Proliferative Activity and Adhesion of Multipotent Stromal Cells to Scaffolds Developed for Tissue-Engineered Constructs
We studied the effect of non-thermal argon plasma on proliferative activity of bone marrow multipotent stromal cellsin vitro. Treatment of stromal cell suspension with pure argon did not affect their proliferation. The cells treated with non-thermal argon plasma and explanted in the treatment medium demonstrated growth inhibition by 30-40% in comparison with the control. Multipotent stromal cells treated with plasma and after centrifugation explanted in normal medium within 12 min demonstrated accelerated growth. The total cell growth from the pellet and supernatant significantly exceeded the control values. We also analyz...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Ultrastructural Changes in Hepatocellular Carcinoma-29 Cells after Treatment with Lithium Carbonate
We studied the effect of lithium carbonate on hepatocellular carcinoma-29 cells in CBA male mice after injection in a dose of 20 mM along the tumor periphery. Transmission electron microscopy revealed a decrease in the volume density of the granular endoplasmic reticulum in the cell cytoplasm and an increase in the total numerical and volume density of autophagosomes and autolysosomes and zones of destruction of intracellular organelles. The ability of lithium carbonate to activate intracellular degradation processes in tumor cells and to stimulate cell death can be used to develop new combined strategies in the chemothera...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research

Administration of Protein Synthesis Inhibitor before Reminder Reverses Amnesia Induced by Memory Reconsolidation Impairment with 5-HT Receptors Antagonist
Administration of 5-HT receptor antagonist to snails trained in conditioned food aversion prior to reminding of the conditioning stimulus caused amnesia. At the early period of amnesia (day 3), injections of protein synthesis inhibitor cycloheximide without reminder or reminder alone were ineffective. At the same time, injections of the inhibitor combined with reminder led to memory recovery; this effect in most animals persisted for at least 10 days. In the rest snails, aversive responses to presentations of the conditioning stimulus persisted for 2 days. Cycloheximide injection and reminder in 10 days after induction of ...
Source: Bulletin of Experimental Biology and Medicine - June 8, 2019 Category: Biology Source Type: research