The Role of Cardiac Opioid Receptors in the Cardioprotective Effect of Continuous Normobaric Hypoxia
We studied the role of opioid receptor subtypes in improvement of the functional state of the heart during reperfusion after adaptation to continuous normobaric hypoxia. To this end, male Wistar rats were subjected to continuous normobaric hypoxia (12% O2). Then, the hearts were isolated and exposed to total 45-min ischemia followed by 30-min reperfusion. Opioid receptor antagonists were added to the perfusion solution prior to ischemia. It was found that continuous normobaric hypoxia reduced the release of creatine phosphokinase into the effluent, increased myocardial contractile force, and decreased the end-diastolic pre...
Source: Bulletin of Experimental Biology and Medicine - April 27, 2020 Category: Biology Source Type: research

Experimental Study of the Biodistribution of New Bone-Seeking Compounds Based on Phosphonic Acids and Gallium-68
We investigate biodistribution of gallium-labeled hydroxyethylidenediphosphonic acid (68Ga-HEDP) and diethylenetriaminepentakis(methylenephosphonic acid) (68Ga-DTPMP) in intact Wistar rats. It was shown that68Ga-DTPMP accumulated mainly in the bone tissue providing high femur/blood and femur/muscle ratios and had high stability in vivo. In contrast,68Ga-HEDP was characterized by low stability and high uptake of radioactivity in blood throughout the study. So68Ga-DTPMP can be considered as a new prospective radiotracer in oncology for imaging bone tissue metastasis by positron emission tomography. (Source: Bulletin of Exper...
Source: Bulletin of Experimental Biology and Medicine - April 25, 2020 Category: Biology Source Type: research

Social and Cognitive Impairments in Rat Offspring after Ultrasound-Induced Prenatal Stress
We studied the possibility of developing an autism model based on chronic prenatal psychological stress caused by variable frequency ultrasound 20-45 kHz. The offspring of female rats stressed during pregnancy demonstrated reduced time of social contacts in the social interaction test, increased anxiety in the open-field test, and memory impairment in the Morris water maze test in comparison with the control (intact) rat offspring. We also found a reducing trend in theBDNF gene expression in the amygdala in males of the experimental group. The results showed the possibility of developing the animal autism model based on pr...
Source: Bulletin of Experimental Biology and Medicine - April 25, 2020 Category: Biology Source Type: research

Specific Features of Interactions between Megakaryocytic and Granulocytic Hematopoiesis Lineages and Myelofibrosis during the Acute Phase of Chronic Myeloid Leukemia, Chronic Lymphocytic Leukemia, and Multiple Myeloma
The specific features of interactions between megakaryocytic and granulocytic hematopoiesis lineages and myelofibrosis were studied in patients with active phase (before treatment) of chronic myeloid leukemia, chronic lymphocytic leukemia, and multiple myeloma. In patients with chronic myeloid leukemia, a direct correlation was found between the severity of both early and advanced myelofibrosis and the number of megakaryocytes in the bone marrow irrespectively of the type of the bone marrow tumor. The parameters of granulocytic hematopoiesis lineage were higher in myelofibrosis. In patients with chronic lymphocytic leukemi...
Source: Bulletin of Experimental Biology and Medicine - April 25, 2020 Category: Biology Source Type: research

Metabolism of a New Antiaggregant, Indolinone Derivative
Cytochrome p450-mediated metabolism of GRS (indolinone antiaggregant) and its effects on activities of cytochrome p450 isoenzymes were studied. Inhibition of 6 isomers of cytochrome p450 in human liver microsomes was studied with the use of specific substrates. It was found that human liver cytochrome p450 enzymes could not induce degradation of GRS and that GRS was not an inductor or inhibitor of cytochrome p450 family members 1A2, 2C9, 2C19, 2D6, 2C8, and 3A4. Hence, clinical use of the prospective antiaggregant would not involve the risk of uncontrolled fluctuations in GRS concentrations in the organism because of inter...
Source: Bulletin of Experimental Biology and Medicine - April 25, 2020 Category: Biology Source Type: research

Ultrastructural Alterations in Granular Neurons of the Dentate Fascia Caused by Intrahippocampal Injection of Beta-Amyloid 1-42
The deposition of beta-amyloid (A β) in the brain is detected in Alzheimer’s disease and during ageing. Until now, ultrastructural studies of changes caused by Aβ in the dentate gyrus are very scarce. The effects of Aβ 1-42 injection into the CA1 field of rat hippocampus were studied by electron microscopy. In 2 weeks after inj ection of aggregated Aβ in low concentrations, destructive changes were seen in the structure of dentate gyrus cells, which consisted in a decrease in the number of dentate gyrus neurons and axo-dendritic synapses. These changes were accompanied by enlargement of the endopl...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Changes in Transcriptional Regulation of Postnatal Morphogenesis of the Adrenal Zona Fasciculata Caused by Endocrine Disruptor Dichlorodiphenyltrichloroethane
We studied the expression of transcriptional factors regulating postnatal morphogenesis of the adrenal zona fasciculata in rats after developmental exposure to endocrine disruptor DDT. It was found that tissue reparation after trophic disorders and cell death triggered by prenatal and postnatal exposure to DDT was accompanied by an increase in the number of Oct4- and Shh-expressing cells forming a pool located outside the regeneration zones and involved in the maintenance of tissue homeostasis in the zona fasciculata. DDT exposure also disrupted the expression of antiproliferative factor Hhex. The data showed that prolifer...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Combined Administration of TLR4 (LPS) and TLR3 (Poly I:C) Ligands to CBA Mice Elevates the Content of Osteogenic MSC by 1.6 Times and Increases Content of Bone Marrow MSC to Intermediate Level between Values Attained by Their Individual Administration
In 1 and 24 h after combined administration of TLR4 (LPS) and TLR3 (Poly I:C) ligands to CBA mice, the content of MSC in bone marrow increased to intermediate value between the levels attained by their individual injections. The content of osteogenic MSC assessed in 24 h postinjection corresponded to the control level in Poly I:C group, decreased in LPS group by 2.5 times relatively to the control, and increased by 1.6 times (relatively to control) after combined administration of the ligands. In 3 h after combined addition of LPS and Poly I:Cin vitro to 12-day-old primary culture of mouse bone marrow stromal cells, the co...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice
The study compared antituberculous efficacy of individual or combined administration of “free” isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose. The therapy started 1 month after contamination of outbred ICR male mice withMycobacterium tuberculosis strain H37Rv. Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity. A minor prodestructive effect of thi...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Immunohistochemical Study of Dark and Progenitor Carotid Body Cells: Artefacts or Real Subtypes?
Postmortem changes occurring in human carotid body were simulated on the Wistar rat model. It was shown that light, dark, and pyknotic (progenitor) subtypes of human carotid body cells are an artifact and cannot be used in clinical practice to study the characteristics of various human diseases. The differences between the control group of healthy individuals and individuals with the various pathologies are most likely due to the different levels of premortal hypoxia that the tissue had been exposed to. Moreover, widespread antigens used in practice were divided into 2 groups by their tolerance to autolysis: stable and uns...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Blood Cytokine Concentration in Rats during Antigenic Treatment after a Single Long-Term Stress Exposure
We studied changes in the blood cytokine profile of rats 3 h, 1 day, and 8 days after acute stress on the model of 24-h immobilization followed by LPS administration (100 μg/kg intraperitoneally). The concentration of proinflammatory cytokines (particularly of IL-1β and TNFα) significantly decreased at the early stage after stress exposure and physiological saline injection, but increased in the follow-up period and practically did not differ or even surpassed the control level by the end of observations. Under these conditions, the blood content of anti-inflammatory cytokine IL-10 increased most significantl...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Activity of Antioxidant Defense Enzymes in Rats with Experimental Allergic Encephalomyelitis
We studied activities of antioxidant system enzymes in tissues of rats with experimental allergic encephalomyelitis. It was shown that the development of pathology is accompanied by deformation of the neurons and axonal degeneration, intensification of free radical oxidation, exhaustion of the reduced glutathione pool, and multidirectional changes in activities of antioxidant enzymes in rat tissues. The observed imbalance in the antioxidant defense system can be associated with excessive glutathione utilization in the glutathione transferase reaction and different severity of the pathological process in the brain and spina...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Blockade of Dopamine D2 Receptors as a Novel Approach to Stimulation of Notch1 + Endothelial Progenitor Cells and Angiogenesis in C57BL/6 Mice with Pulmonary Emphysema Induced by Proteases and Deficiency of α1-Antitrypsin
We studied the effects of spiperone, a selective blocker of dopamine D2 receptors, on the model of pulmonary emphysema provoked by administration of elastase and D-galactosamine hydrochloride to female C57BL/6 mice and characterized by activation of proteases in the lungs and systemic deficiency of its inhibitor α1-antitrypsin. In this model, spiperone prevented the development of inflammatory reaction and reduced the area of emphysematous expanded alveolar tissue. The expression of angiogenic marker CD31 in the lungs increased under these conditions. Regeneration of the damaged microvascular bed under the action of ...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Unfavorable Humoral Factors for Spermatozoon Survival in Vaginal Medium
The effects of vaginal secretion, its antibacterial peptide fraction, albumin, and bacterial metabolites on the spermatozoon membranes were studied. Vaginal secretion was collected from healthy women; the fraction of antibacterial peptides was isolated with the use of membrane filter; electrophoretically pure BSA served as albumin; bacterial metabolites were isolated from supernatants of culturedCandida albicans yeast andE. coli andStaphylococcus aureus bacteria. Antibacterial activities of the preparations were evaluated by spectrophotometry and microscopy. Albumin in a concentration close to its serum concentration (50 m...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Analysis of the Pharmacokinetics of Suppository Forms of Bacteriophages
Pharmacokinetics of suppository forms of bacteriophages was studied on male Chinchilla rabbits. Suppositories with various composition of bacteriophages were administered onceper rectum to rabbits, and the presence of phage particles was estimated in the blood, urine, and feces over 24 h. Pharmacokinetic study showed that the phages were detected in the blood, urine, and feces at various terms of the experiment irrespective of the size of viral particles, which confirmed the possibility of their systemic effects after rectal administration. Thus, the use of suppository form of bacteriophages can ensure the presence of phag...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Expression of Metalloproteinases and Type I and III Collagens during Healing of Excisional Skin Wound on the Abdomen and Back in Rats
The study was carried out using a novel rat model developed in our laboratory, namely16 mm diameter circular excisional wounds were generated on the abdomen which resulted in minimal scarring. Restoration of the skin integrity was completed by day 60 after the wounding surgery. By this time, regenerates on the abdomen were stronger than on the back (at, respectively, 58 and 17.4 % of the tensile strength of the intact skin at corresponding location) and the ratio of type I and type III collagens in regenerates on the abdomen reached the level of intact skin at the same location. On days 3 to 14, the ratio ofMmp9/Timp1 expr...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Proliferative Activity of Mouse Splenocytes in Physiological Pregnancy and in Models of Spontaneous and Muramylpeptide-Dependent Abortions
Spontaneous proliferative activity of splenocytes in female CBA mice and the response of these cells to antigens of allogeneic male BALB/c and DBA/2 mice in a mixed splenocyte culture were evaluated by3H-thymidine incorporation in different pregnancy models. ♀CBA×♂BALB/c mating was used for modeling physiological pregnancy. Spontaneous abortions were reproduced by abortion-prone ♀CBA×♂DBA/2 mating. In order to simulate immunostimulant-induced and immunostimulant-potentiated abortions, 0.83 mg/kg muramyl dipeptide β-heptylglycoside was intrape ritoneally injected to CBA females mated with BALB/c or ...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Rat Blood Leukocytes after Intravenous Injection of Chitosan-Modified Magnetic Nanospheres
Nanosized magnetite particles (magnetic nanospheres) are a prospective basis for creation of new diagnostic and therapeutic agents. The structure of blood leukocytes and the leukocytic formula are studied in adult rats over a period of 120 days after a single intravenous injection of chitosan-modified nanosized magnetite particles. No effects of chitosan-modified magnetic nanospheres on the structure of rat blood leukocytes are detected. Injection of suspension of chitosan-modified magnetite nanospheres is associated with an increase in the levels of monocytes, segmented and stab neutrophils, and a decrease in lymphocyte c...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Characterization of Sperm of White Rats at Exposure of Bisphenol A and Triclosan
We studied the effect of unstable ubiquitous endocrine disruptors (bisphenol A and triclosan) on the reproductive function of mature male Wistar rats. Animals of both experimental groups received bisphenol A and triclosan (homogeneity>97%) in a dose of 200 mg/kg daily for 2 months with food. The concentrations of disruptors were measured directly in the testicular tissue by gas chromatography with mass spectrometry (GC-MS). In both experimental groups, the concentrations of bisphenol A and triclosan in the testicular tissue significantly differed from the control (intact animals). Animals exposed to bisphenol A signific...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Response of the Hemostatic System to Acute Vital Stress in Rats
We studied the effect of acute vital stress on the parameters of the hemostasis system in young and old Wistar rats. The psychotraumatic effect was simulated as a situation of emotional stress at the sight of a predator (Indian python) catching and eating a conspecific. In young rats, acute vital stress was followed by inhibition of platelet aggregation, hypercoagulation by the external pathway and final stages of plasma hemostasis, and a decrease in fibrinogen and antithrombin III levels against the background of fibrinolysis activation. In old animals, increased platelet count with reduced aggregation capacity, hypocoagu...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

First Experience of Sutureless Redo on Mitral Valve Using Valve-in-Valve Technique: Two-Stage Implantation on a Large Animal
Sutureless implantation of the mitral valve bioprosthesis using the valve-in-valve method was performed on a large animal (sheep). According to the results of a two-stage implantation (primary implantation of a xenopericardial 26-mm framed bioprosthesis and reimplantation of the developed 23-mm bioprosthesis), minor changes in quantitative indicators were revealed: an increase in the transprosthetic gradient by 1.3 mm Hg and a decrease in the area of the mitral orifice by 21.6%. Considerable reduction in the intervention time by 18 min was achieved (by 40% in comparison with the primary prosthesis). The absence of adverse ...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Parameters of Experimental Activity in the Study of the Cognitive Abilities of Rhesus Monkeys ( Macaca mulatta ) and Baboons ( Papio hamadryas )
We compared experimental activity, behavioral activity in the experiment, of rhesus monkeys (Macaca mulatta) and baboons (Papio hamadryas). Baboons showed higher levels of experimental activity. The contrast in the activity between the species is probably associated with species-specific characteristics of the behavior and different ability to adapt under new conditions. In particular, they may be the result of lower plasticity of rhesus monkeys with respect to experimental conditions. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Changes in Membrane and Threshold Potentials of Command Neurons in Terrestrial Snail during Development of a Conditioned Situational Defensive Reflex
Changes of the electrical characteristics of command neurons of defensive behavior caused by the development of a conditioned situational defensive reflex were studied experimentally underin vitro conditions on preparations of the nervous system of snails. After learning, the membrane and threshold potentials of command neurons LPa3 and RPa3 significantly decreased and excitability of the studied neurons increased. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Effect of Platinum Nanoparticles on the Structure of Protein Molecules and Regulation of the Tone of Brain Vessels in Experimental Animals
The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. The study of solid phase structures of blood serum was conducted by the method of cuneiform dehydration. The results showed that injection of platinum nanoparticles significantly aff...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Method of Surgical Treatment of Spine Injury with the Use of Extracorporeal Navigation System Based on Additive Technologies
We present a method of minimally invasive transcutaneous insertion of screws using a prefabricated extracorporeal navigation system using additive technologies (based on primary data obtained from the DICOM package in multi-detector computed tomography of the affected spine segment) according to the principle of personalized medicine. The method was tested on 10 dogs of different breeds with generally similar mechanism of trauma and typical consequences that led to fracture and dislocation of one of the lumbar vertebrae. In all animals, a positive treatment outcome of different degrees was achieved. Regression of the neuro...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Distribution of Ferrihydrite Nanoparticles in the Body and Possibility of Controlling Them in an Isolated Organ by a Permanent Magnetic Field
We studied the distribution of ferrihydrite nanoparticles isolated from bacteriaKlebsiella oxytoca in the whole bodyin vivo and in a cultured isolated organ (liver). The possibility of controlling these nanoparticles in the body using a magnetic field was assessed. One hour after intravenous injection of ferrihydrite nanoparticles to mice, their accumulation was observed in the liver, lungs, and kidneys. Experiment with cultured isolated rat liver showed that these nanoparticles can be controlled by a magnetic field and the influence of magnetic nanoparticles on the liver over 1 h does not lead to destruction of liver cell...
Source: Bulletin of Experimental Biology and Medicine - April 23, 2020 Category: Biology Source Type: research

Ammonium Salts Increase Physical Performance and Reduce Blood Lactate Level in Rats in a Model of Forced Swimming
We compared the effects of two doses of ammonium chloride and ammonium carbonate (10 and 20 mg/kg) on the duration of swimming and blood lactate level. Ammonium chloride in a dose of 20 mg/kg was more efficient than in a dose of 10 mg/kg. The efficiency of ammonium carbonate in a dose of 10 mg/kg was similar to that of ammonium chloride in a dose of 20 mg/kg. Increasing the dose of ammonium carbonate to 20 mg/kg led to a decrease in the duration of swimming. On the last day of the experiment, lactate level in 5 min after exhausting load was maximum in control rats, while in rats treated with 10 mg/kg ammonium carbonate and...
Source: Bulletin of Experimental Biology and Medicine - April 6, 2020 Category: Biology Source Type: research

The Levels of Monoamines and Their Metabolites in the Brain Structures of Rats Subjected to Two- and Three-Month-Long Social Isolation
The levels of monoamines and their metabolites in the brain structures of adult Wistar rats subjected to post-weaning social isolation for 2 and 3 months were analyzed by HPLC with electrochemical detection. We have previously shown that these rats consistently demonstrate increased aggressiveness and, as a rule, impairment of short-term habituation. Two-monthlong social isolation was accompanied by a reduction in serotonin content and its increased turnover judging from the 5-HIAA/5-HT ratio in the hippocampus; three-month-long isolation was associated with increased levels of serotonin and reduction in its turnover in th...
Source: Bulletin of Experimental Biology and Medicine - April 6, 2020 Category: Biology Source Type: research

Contribution of Hydrogen Sulfide to Dilation of Rat Cerebral Arteries after Ischemia/Reperfusion Injury
The study examined the effect of H2S on the tone of cerebral arteries in rats after global cerebral ischemia/reperfusion injury and cooperation between NO and H2S in the control over cerebral circulation during the postischemic period. In control sham-operated and in experimental rats with ischemia/reperfusion injury, the diameter of pial arteries was repeatedly measuredin vivo under a light microscope after removal of parietal bone and dura mater in 1 h and in 7 days after the surgery. The study established that H2S is an important signaling molecule in pial arteries, where it is responsible for vasodilation. Interaction ...
Source: Bulletin of Experimental Biology and Medicine - April 6, 2020 Category: Biology Source Type: research

Effect of Infrared Low-Intensity Laser Irradiation on Lipid Peroxidation under Conditions of Experimental Circulatory Hypoxia of Visual Analyzer
The physiological stress modeled by circulatory hypoxia activates LPO processes in various tissues. In posthypoxic period, the infrared low-intensity laser irradiation significantly decreased the chemiluminescence parameters in blood plasma, normalized the retinal levels of diene and triene conjugates, and decreased MDA in the rat brain attesting to the correcting effect of this irradiation during various types of physiological stresses. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - April 6, 2020 Category: Biology Source Type: research

The Relationship of Immunoexpression of Ki-67 and Hsp70 with Clinical and Morphological Parameters and Prognosis of Papillary Thyroid Cancer
We studied the correlations of the expression of Ki-67 proliferation marker and localization of heat shock protein Hsp70 in tumor cells of papillary thyroid cancer with some clinical and morphological parameters and a 10-year survival prognosis. High variability of the proliferative activity (by Ki-67 index) of tumor cells was revealed. It was found that Hsp70 protein has different localization in tumor cells: cytoplasmic, nuclear, nucleolar, and mixed. Nuclear translocation of the Hsp70 protein correlated with the stage of the process and the prognosis of the disease, but did not correlate with sex, tumor size, capsule in...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Energy Metabolism in Cellular Regenerative Processes: Focus on PPAR α
Reduced expression of the key regulator of cardiac metabolism, transcription factor PPAR α, in surgical samples of the auricles from patients with coronary heart disease and heart failure was detected by real-time quantitative PCR. These changes indicate reduced activity of this factor and a shift of energy metabolism from oxidative phosphorylation to glycolysis typical of dedifferenti ated cells. Electron microscopy revealed dedifferentiated cardiomyocytes with disassembled contractile apparatus and disorganized sarcomeres. In the examined specimens from patients with heart failure, severe myocardial fibrosis was re...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Peculiarities of Histological Structure of Pulmonary Veins in Patients with Total Anomalous Pulmonary Venous Drainage as Morphological Substrates Relating to Formation of Postoperative Pulmonary Venous Obstruction
The histological and immunohistochemical methods were employed to examine the peculiarities of histological structure of pulmonary veins and left atrium of the heart in norm and in various types of total anomalous drainage of pulmonary veins. In contrast to normal pulmonary vein covered with external multiple musclelayers (myocardial sleeve), such sleeve is absent in veins that have no connection with the left atrium irrespective of the type of the defect. In patients with total anomalous pulmonary venous drainage, the structure of left atrium was heterogeneous featuring either the presence or absence of inner angiomural l...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Differential Expression of Local Immune Response Genes in the Vagina: Implication for the Diagnosis of Vaginal Infections
Transcription profiles of genes of local immune response were determined in the vagina of women with bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidosis for detection of the most specific immune markers for these vaginal infections. Laboratory diagnosis of the vaginal infections was performed microscopically; the inflammatory reaction in the vagina (leukorrhea) was defined as the presence of>10 white blood cells per field of view. Transcription profiles ofIL1b,IL10,IL18,TNF α,TLR4,GATA3, andCD68 were determined using reverse-transcription quantitative real-time PCR. The strongest predictors of aero...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Effect of Vanadium Complex with Enzymatic Hydrolysate of Soy Protein on Carbohydrate and Lipid Metabolism Disorders in Male Wistar Rats
The effects of vanadium complex with enzymatic hydrolysate of soy protein (V-EHSPI) were studied in male Wistar rats with induced disorders of carbohydrate and lipid metabolism. The content of vanadium (IV) in the studied complex was 15.8 mg/g dry product. High-lipid high-carbohydrate diet was used to induce disorders of lipid and carbohydrate metabolism. Addition of vanadium in three different doses to the diet over 100-day experiment reduced body weight gain and the levels of glucose, insulin, leptin, and triglycerides. V-EHSPI produced beneficial effects on carbohydrate and lipid metabolism even in a dose 5 μg/kg bod...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Cytokines of the Lymph and Blood Serum as Markers of Oncogenesis and Efficiency of Therapy in Chemically Induced Breast Cancer in Wistar Rats
We studied the levels of serum and lymph cytokines involved in the pathogenesis of BC. BC was induced by injection of N-methyl-N-nitrosourea to Wistar rats. The animals underwent surgery, or received polychemotherapy (cyclophosphamide, methotrexate, and 5-fluorouracil), or surgical treatment was combined with polychemotherapy; in a special group, Panagen (fragmented DNA) was added to polychemotherapy. Cytokine concentration in the lymph was measured using Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad). In rats with BC, the content of most studied cytokines (IL-1 β, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Prognostic Role of Matrix Metalloproteinases 2, 7, 8, 9 and Their Type 1 Tissue Inhibitor in Blood Serum of Patients with Kidney Cancer
The study compared the levels of MMP-2,7,8,9, and TIMP-1 in blood serum of healthy people (N=97) and patients with primary renal cell carcinoma (N=93) to assess relevance of these markers to prognosis of overall survival of these patients, which were followed-up over 1 to 45 months (median 26 months). To evaluate the survival with theKaplan —Meier estimator, the median values of examined markers in the total group of patients were taken as the threshold levels. This estimator showed that the high levels of serum MMP-7 and MMP-8 were indicative forunfavorable prognosis in the total group of patients with renal cell ca...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Structural Changes in the Lungs and Liver of Mice with Experimental Tuberculosis Treated with Liposome-Encapsulated Dextrazide
Male BALB/ с mice were intravenously infected withMycobacterium tuberculosis H37Rv (0.5 ml of 2-week culture). One month later, treatment with liposome-encapsulated dextrazide (LEDZ, a conjugate of isonicotinic acid hydrazide (INH) and 40 kDa oxidized dextran encapsulated in phosphatidylcholine liposomes), INH, or a combination of LEDZ with INH was started. The doses of LEDZ (liposome suspension) and INH were 0.025 ml/10 g body weight and 5 mg/kg body weight, respectively. All the substances were administered 2 times a week via inhalation or intraperitoneally (a total of 40 doses). We studied the number and the size of tu...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Effects of Catechin on Activity of Angiotensin-Converting Enzyme and Generation of Reactive Oxygen Species in Rat Aorta
Activity of angiotensin-converting enzyme (ACE) and intensity of ROS generation in the aorta were studied in male Wistar rats intraperitoneally injected with a mixture of (+) and (-) stereoisomers of catechin. ACE activity in aortal segments was evaluated by hydrolysis of hippuryl-histidine-leucine; ROS generation was measured by oxidation of dichlorodihydrofluorescein. The dynamics of ACE activity and intensity of ROS generation in the aorta after catechin administration and their dependence on the catechin dose were studied. The effects of dihydroquercetin and fucoidin on the studied parameters were analyzed. Catechin in...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Cardioprotective Effects of Metalloproteinase Inhibitor 1-({4-[(4-Chlorobenzoyl)amino]phenyl}sulfonyl-L-Proline in Modeled Acute Myocardial Infarction
Cardioprotective effect of 1-({4 [(4 chlorobenzoyl)amino]phenyl}sulfonyl-L-proline (compound AL-828) was studied in rats with modeled acute myocardial infarction. The test compound was administered intragastrically in a dose of 30 mg/kg/day for 3 days prior to infarction modeling. Metalloproteinase inhibitor antibiotic doxycycline served as the reference drug and was administered in a dose of 40 mg/kg/day by the same schedule. It was shown that AL-828 similar to doxycycline significantly reduced the intensity of myocardial remodeling and maintained the inotropic function of the myocardium in the acute phase of myocardial i...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Structural and Functional Changes in the Mesenteric Lymph Nodes in Humans during Aging
Morphometric analysis of structural and functional changes in the human mesenteric lymph nodes during aging revealed the development of fibrous connective tissue, fragmentation of the lymphoid parenchyma, the absence of follicles with germinal centers, and a decrease in the level of lymphocyte proliferation, which was confirmed by the absence of Ki-67+ cells. The paracortical zone lacked CD4+ T helpers that regulate both cellular and humoral immunity. High content of plasma cells and eosinophilic granulocytes in the medullary cords and sinuses reflects the development of autoimmune processes associated with a decrease in t...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Morphological Study of Mesenteric Lymph Nodes in Rats with Experimental Breast Cancer Treated with Fragmented Double-Stranded DNA
We performed a morphometric analysis of mesenteric lymph nodes in rats with breast cancer induced by administration of N-methyl-N-nitrosourea. The volume of the paracortical zone and the number of mature plasma cells in the medullary sinuses were increased and the volume of lymphoid nodules with germinal centers and the number of macrophages were decreased in the group with tumor resection and chemotherapy in comparison with untreated rats with breast cancer. In rats receiving fragmented human double-stranded DNA in combination with adjuvant therapy, the volume of marginal and medullary sinuses and the number of small lymp...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines
The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. Accordi...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Effects of Humic Acids, Isolated from High-Moor Pine-Peat Moss-Cotton Grass Peat on the Production of Cytokines by Mouse and Human Immunocompetent Cells and on Humoral Immune Response
A course of treatment with humic acids extracted with sodium pyrophosphate from high-moor pine-peat moss-cotton grass peat improves humoral immune response of C57BL/6 mice, stimulates the production of TNF α, IL-1β, and IL-12 by the animal peritoneal macrophages and the production of IFNγ and TNFα by donor peripheral blood mononuclears, causing no changes in the production of IL-10in vitro. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Peptide KE in Human Proteome
Peptide KE exhibits immunoprotective, geroprotective, and oncostatic activities and stimulates functional activity of fibroblasts. The KE motif is present in amino acid sequences of some cytokines and peptide hormones functionally similar to KE peptide. However, the relationship between the presence of KE motif and protein functions on the scale of known human proteome has not yet received sufficient attention. The incidence of bioregulatory peptide KE in proteins of various functional groups constituting human proteome is studied. The study is carried out with the use of the available data on the human proteome (UniProt p...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus
We studied virus-inhibiting activity of Baikal skullcap (Scutellaria baicalensis) flavonoids against tick-borne encephalitis virus using various model schemes. The half-maximum cytotoxic concentration (CC50) for the plant extract was found (363.9 ±58.6 μg/ml). Based on the CC50 and IC50, selective index (SI) was calculated for viricidal (53.4), preventive (50.5), and direct antiviral actions (39.1) and for-intracellular replication of the virus (40.4). Suppression of virus reproduction ≥2.0 lg TCID50 was observed at extract concentration ≥5 μg/ml (viricidal effect), ≥11.2 μg/ml (preventive and dire...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Correction of Lipid Metabolism Disorders in Diabetes Mellitus with Peptide Drugs
We studied the effect of peptide drugs deltalicin and Semax on lipid metabolism disturbances in diabetes mellitus. Diabetes mellitus was modeled by single injection of streptozotocin (45 mg/kg) and rats with blood glucose ≥12 mmol/liter were selected for the further experiments. Deltalicin in a dose 100 μg/kg and Semax in a dose 200 μg/kg as well as sulodexide corrected lipid metabolism disorders: the content of total cholesterol, triglycerides, LDL, index of atherogenicity decreased and HDL concentration increas ed. Deltalicin produced more potent effect on lipid metabolism in rats with diabetes mellitus than sul...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Effect of Rheological Conditions of the Environment on Adaptive Behavior of Lactobacilli in Microculture
Adaptive behavior of lactobacilli in cultured in microchambers of different design was analyzed under a light microscope. We found that the time of appearance of first-generation cells for the studied strains of lactobacilli differed in chambers with different rheological properties (stationary and flow-through). The results of our experiments suggest that the development of populations of lactobacilli is regulated by autometabolites of different physiological modalities directly from the very first cell generations. Populations of lactobacilli are under the control of autometabolites at the initial stages of interaction w...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Mechanism of Natriuretic Effect of Oxytocin
In rats, intramuscular injection of oxytocin (0.25 nmol/100 g body weight) increased sodium excretion from 19 ±5 to 120±11 μmol/min. A significant correlation (p
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research

Acclimatization to Middle Attitude Hypoxia Masks the Symptoms of Experimental Posttraumatic Stress Disorder, but Does Not Affect Its Pathogenetic Mechanisms
The effects of acclimatization to middle attitude hypoxia on the resistance to acute emotional stress were studied on the model of posttraumatic stress disorder in rats. Anxyolitic, but not anxiogenic effect was observed in acclimatized rats. However, acclimatized rats with posttraumatic stress disorder were characterized by hypofunction of the pituitary —adrenocortical axis, which is typical of this pathology, and reduction in corticosterone/dehydroepiandrosterone ratio. At the neuroendocrine level, up-regulation of glucocorticoid receptors and a decrease in the level of corticotropin-releasing hormone in the hippoc...
Source: Bulletin of Experimental Biology and Medicine - April 4, 2020 Category: Biology Source Type: research