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Intensity of 40K Decay Fluctuations an Indicator of an Environmental Factor Determining Infradian Rhythms of Mouse Motor Activity, Circulating Cortisol in Rabbits, and Proliferation of L-929 Cultured Cells
The multi-day dynamics of the intensity of40K decay fluctuations was compared with motor activity of laboratory mice, circulating cortisol in rabbits, and proliferation of fibroblast-like cultured cells L-929. A positive correlation was established between the intensity of decay fluctuations on the one hand, and total daily motor activity in mice and plasma cortisol level in rabbits, on the other hand. In addition, a negative correlation was observed between40K decay fluctuations and proliferative activity of the cultured cells. Interestingly, these close correlations were observed not only against the background 4-day rhy...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Angiogenin as a Possible Mediator of Macrophage-Mediated Regulation of Fibroblast Functions
We studied angiogenin production by human macrophages and evaluated the role of this factor in the macrophage-mediated regulation of fibroblasts. All macrophage subtypes, and especially the efferocytosis-polarized macrophages, M2(LS), actively produced angiogenin. Exogenous recombinant angiogenin dose-dependently enhanced the proliferation and differentiation of dermal fibroblasts. The addition of the angiogenin inhibitor to fibroblasts cultures suppressed the stimulating effect of exogenous angiogenin or M2(LS) conditioned media. These findings indicate the involvement of angiogenin in the macrophage-mediated paracrine re...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Efficacy of Raphamin against Pneumococcal Infection: a Preclinical Study
The aim of the study was to evaluate the activity of Raphamin in a model of non-lethal pneumococcal infection caused byStreptococcus pneumoniae 3 in BALB/c mice. The drug or placebo was administered intragastrically 3 days prior to infection, 2 h before and 2 h post infection, and then for 3 full days, alone or in combination with antibiotic (amoxicil-lin/clavulanic acid). Raphamin monotherapy significantly decreased bacterial load in the lungs in comparison with placebo (p<0.05) which was comparable to the effect in antibiotic alone or combined with Raphamin. Raphamin prevented reproduction ofStreptococcus pneumoniae i...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Morphological and Molecular Biological Features of the Systemic Inflammatory Response in Old Wistar Rats with High and Low Resistance to Hypoxia
Morphological and molecular biological features of LPS-induced systemic inflammatory response were assessed in old male Wistar rats with high (HR) and low (LR) resistance to hypoxia. The systemic inflammatory response was modeled by intraperitoneal injection ofE. coli O26:B6 LPS; the animals were sacrificed after 6 h. In histological sections, the number of neutrophils in the interalveolar septa and the area of necrosis in the liver were determined. The expression levels ofHif1a,Hif2a,Nfkb,Vegf,Il1b,Il6,Il10, andTgfb mRNA in the liver and serum concentrations of HIF-1 α and IL-1β were determined. In 4-6 h after LPS injec...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

In Vivo and In Vitro Analysis of Toxicity of a Prospective Magnetic Resonance Contrast Agent Based on Arabinogalactan and Gadolinium Nanocomposite
We studied the cytotoxic effect of gadolinium nanocomposite on cultured mouse fibroblasts 3T3-SV40 and histological changes in the liver tissue of albino rats after its administration. Forin vitro experiment, gadolinium nanocomposite on the natural matrix of arabinogalactan (nGd-AG) was dissolved in DMEM nutrient medium to concentrations of 0.005, 0.02, 0.5, 2, and 5 mM. Inin vivo experiment, a nGd-AG solution was orally administered to rats through a tube in a dose of 500 μg Gd/kg in 1 ml of 0.9% NaCl for 10 days. The pattern and degree of influence of the gadolinium nanocomposite on the studied cell culture depended on ...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Ultrastructural Reorganization of Endotheliocytes of Pulmonary Blood Capillaries in COVID-19
The ultrastructural organization endotheliocytes of pulmonary blood capillaries in COVID-19 was studied on autopsy material using electron microscopy. Swelling of the cytoplasm and mitochondria with destruction of the cristae, dilation of the Golgi complex cisternae, a decrease in the volume density of the luminal and basal caveolae and free transport vesicles, an increase of the rough endoplasmic reticulum, as well as the presence of elements of coronavirus replication (reticulovesicular structures, zippered endoplasmic reticulum, electron-dense particles in the Golgi cisternae, and vacuoles with viral particles) were rev...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Influence of Chemotherapy on the Relationship between MicroRNA Levels in the Lymph with Thymus Structure in Experimental Breast Cancer
The relationship between the expression levels of microRNAs (miR-21, miR-221, miR-222, and miR-429) and morphometric parameters of the thymus was studied in female Wistar rats with breast cancer receiving and not receiving chemotherapy. In breast cancer, the following positive relationships were revealed: miR-221 in the subcapsular zone of the cortical substance with medium lymphocytes, miR-221 in the central part of the cortical substance with immunoblasts and macrophages, miR-429 in the subcapsular zone of the cortical substance with mitotically dividing cells, miR-429 in the central part of the medullary substance with ...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Analysis of General Toxicity of Ergoferon
For antibody-based drugs, it is important and relevant to study their toxic effects, which can often become limiting when prescribing this type of therapy. General toxicity of antiviral drug Ergoferon based on technologically processed antibodies was studied on sexually mature animals. Analysis of acute toxicity showed the absence of lethal outcomes when the drug was administered to adult rats at the maximum tolerated doses. In a study of repeated dose toxicity, no adverse effects of the drug were detected. (Source: Bulletin of Experimental Biology and Medicine)
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Effect of Short PSG Peptides on Inflammatory Markers in Allogeneic Bone Marrow Cell Transplantation in Wistar Rats
Short linear peptide fragments of placental trophoblastic β1-glycoprotein (PSG) (YECE, YQCE, YVCS, and YACS) were studied in the context of their immunomodulatory effects at the level of inflammatory markers. The original host-versus-graft model was used in male Wistar rats without prior conditioning of recipient bone marrow. A composition of PSG peptide fragments was injected to animals after allogeneic transplantation of bone marrow cells in a dynamic experiment, inflammatory markers α1-acid glycoprotein (AGP, orosomucoid), α2-macroglobulin (α2M) were assayed by ELISA, and biochemical parameters (total protein, gluco...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Lithium-Modified Sorbent: Effects on Hemostatic Responses In Vitro
The effects of a lithium-modified sorbent based on aluminum oxide and polydimethylsiloxane (Al2O3@PDMS/Li) and a lithium-free sorbent (Al2O3@PDMS) on some indicators characterizing blood clotting under hemosorption conditions were comparedin vitro. Sorbent Al2O3@PDMS/Li had significantly lower reactogenic effect on the blood passed through the column than the sorbent without lithium. This was seen from the degree of platelet reduction (66 ×109vs 19 ×109/liter) as well as a less pronounced hypercoagulation shift in chronometric indicators. In contrast to lithium-free sorbent, Al2O3@PDMS/Li demonstrated the ability to redu...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Molecular Genetic Characterization of Streptococcus pyogenes Strains Isolated from Patients with Various Manifestations of Streptococcal Infection
In 82 clinical strains ofStreptococcus pyogenes (group A streptococci) isolated from patients with various manifestations of streptococcal infection,emm-typing revealed 27emm-types (n=77) with a predominance ofemm-89 (n=15; 18%),emm-75 (n=9; 11%), andemm-1 (n=6; 7%); typesemm-3,emm-12, andemm-58 (n=4; 5% each) were found with almost equal frequency; other types were less common. The superantigen genesspeC,speG,speH,speI,speJ,speK,speL,speM,smeZ, andSSA were identified inS. pyogenes strains using multiprimer PCR; the genes of the superantigen SpeA and cysteine proteinase SpeB were detected using real-time PCR. All the studi...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Associations of Fatty Acid Profile with Abdominal Obesity in Men
The content of individual unsaturated fatty acids in blood plasma (measured by HPLC) and their association with abdominal obesity in a group of men (mean age 52.2 years) was analyzed. The abdominal obesity was diagnosed according to the criteria of the All-Russian Scientific Society of Cardiology (waist circumference>94 cm). Men with abdominal obesity had higher levels of ω-6 γ-linolenic and dihomo-γ-linolenic acids, as well as ω-3 eicosapentaenoic and docosahexaenoic acids. They also had significantly elevated plasma levels of triglycerides and glucose and lower levels of HDL. Using single-factor ROC analysis we de...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Ultrastructure of Myofascial Trigger Points of Skeletal Muscles of Rats after Photobiostimulation with Low-Intensity Red Light
The effectiveness of low-intensity red light on myofascial trigger points in skeletal muscle of mature rats was evaluated by electron microscopy and high-resolution respirometry. The revealed changes in mitochondrial ultrastructure and activity of the respiratory chain enzymes indicate the development of hypoxia in the simulation area. Under the influence of low-intensity red light on myofascial trigger points, a decrease in the number of destructively altered muscle fibers and stimulation of mitochondrial respiration were found. These findings indicate intracellular regeneration and the stimulating effect of low-intensity...
Source: Bulletin of Experimental Biology and Medicine - October 20, 2023 Category: Biology Source Type: research

Allisartan Isoproxil Promotes Uric Acid Excretion by Interacting with Intestinal Urate Transporters in Hyperuricemic Zebrafish (Danio rerio)
To evaluate the urate-lowering effect and potential drug targets of antihypertensive agent allisartan isoproxil (ALI) and its bioactive metabolite EXP3174, we developed an acute hyperuricemic zebrafish model using potassium oxonate and xanthine sodium salt. Losartan potassium served as the positive control (reference drug). In this model, ALI and losartan potassium exerted a greater urate-lowering effect than EXP3174 indicating that the latter is not the critical substance for elimination of uric acid. The quantitative real-time PCR showed that ALI upregulates the expression of intestinal urate transporters genesABCG2,PDZK...
Source: Bulletin of Experimental Biology and Medicine - October 19, 2023 Category: Biology Source Type: research

The Use of Microencapsulated Autologous Thrombi for Modelling Chronic Thromboembolic Pulmonary Hypertension in Rats
Here we developed a model of chronic thromboembolic pulmonary hypertension (CTEPH) using repeated intravenous administration of microencapsulated thrombi with a controlled rate of biodegradation. Autologous thrombi encapsulated in alginate microspheres with a diameter of 190 ±48 μm were intravenously injected to rats 8 times every 4 days. In the comparison group, nonmodified thrombi were injected. After 6 weeks, a significant increase in systolic pressure in the right ventricle, a decrease in exercise tolerance, and an increase in the index of vascular wall hypertroph y were revealed in the group receiving injections of ...
Source: Bulletin of Experimental Biology and Medicine - October 19, 2023 Category: Biology Source Type: research