Bioscience, Biotechnology, and Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Relationship between microorganisms and volatile components in each fermentation process in the kusaya gravy that plays an important role in the manufacturing of kusaya, a traditional Japanese fermented fish product
In this study, we aimed to investigate the relationship between the microbiota and volatile compounds involved in the manufacturing and management of kusaya gravy. 16S rRNA gene-based amplicon sequencing revealed that the microbiota in kusaya gravy was significantly different between the two islands, and the microbiota hardly changed during each fermentation process. Gas chromatography-mass spectrometry analysis also revealed that the volatile components were strongly related to the microbiota in kusaya gravy, with Hachijojima samples containing sulfur-containing compounds and Niijima samples containing short-chain fatty a...
Source: Bioscience, Biotechnology, and Biochemistry - October 10, 2023 Category: Biochemistry Authors: Kosuke Kita Ryosuke Unno Takahiro Osada Hiromori Yoshiyama Sachiko Masaki Sakura Nogimura Minenosuke Matsutani Morio Ishikawa Toshihiro Suzuki Source Type: research

Differences in iron balance observed with dietary cholic acid supplementation and marginal iron deficiency in rats
This study revealed an involvement of 12-hydroxylated bile acids in regulation of hepatic iron concentration under MID condition.PMID:37813822 | DOI:10.1093/bbb/zbad140 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - October 9, 2023 Category: Biochemistry Authors: Natsuki Kubota Shota Hori Satoshi Ishizuka Source Type: research

Degradation of DDT by γ-HCH dehydrochlorinase LinA
In this study, we found that γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase LinA from a γ-HCH-degrading bacterium, Sphingobium japonicum UT26, converts DDT to 1,1-dichloro-2,2-bis(4-chlorophenyl)-ethylene (DDE). Because of the weak DDT degradation activity of LinA, we could not detect such activity in UT26 cells expressing LinA constitutively. However, the linA-deletion mutant of UT26 harboring a plasmid for the expression of LinA, in which LinA was expressed at a higher level than UT26, showed the DDT degradation activity. This outcome highlights the potential for constructing DDT-degrading sphingomonad cells throug...
Source: Bioscience, Biotechnology, and Biochemistry - October 5, 2023 Category: Biochemistry Authors: Kafayat Olaide Yusuf Habibullah Ren Ito Leonardo Stari Kouhei Kishida Yoshiyuki Ohtsubo Eiji Masai Masao Fukuda Keisuke Miyauchi Yuji Nagata Source Type: research

Chemical screening of inhibitors specific for MdDOX-Co that cause an apple columnar tree-shape
Biosci Biotechnol Biochem. 2023 Oct 3:zbad142. doi: 10.1093/bbb/zbad142. Online ahead of print.ABSTRACTMdDOX-Co, the ectopic expression of which is considered to cause the columnar tree shape, belongs to the 2-oxoglutarate-dependent dioxygenase (2ODD) family. It adds a hydroxyl group to position 12 of gibberellins. However, the 2ODD enzymes related to gibberellin biosynthesis and catabolism are phylogenetically distinct from MdDOX-Co. Thus, it is possible that substrates other than gibberellins exist in MdDOX-Co. To identify the previously unidentified substrate(s) of MdDOX-Co, we searched for MdDOX-Co-specific inhibitors....
Source: Bioscience, Biotechnology, and Biochemistry - October 4, 2023 Category: Biochemistry Authors: Keisuke Okamoto Taiki Inoue Tsunesato Nagano Sho Miyazaki Ikuo Takahashi Tadao Asami Kazunori Okada Kazuma Okada Masatoshi Nakajima Source Type: research

Direct screening for effective shRNA with a single mismatch in human cells without laborious cloning
Biosci Biotechnol Biochem. 2023 Oct 4:zbad143. doi: 10.1093/bbb/zbad143. Online ahead of print.ABSTRACTWe have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.PMID:37793878 | DOI:10.1093/bbb/zbad143 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - October 4, 2023 Category: Biochemistry Authors: Mayu Tachibana Nazumi Ehara Shunya Tanikawa Akira Tachibana Source Type: research

The effect of Dunaliella tertiolecta supplementation on diet-induced obesity in UCP1-deficient mice
Biosci Biotechnol Biochem. 2023 Sep 30:zbad138. doi: 10.1093/bbb/zbad138. Online ahead of print.ABSTRACTWe previously demonstrated that dietary supplementation with Dunaliella tertiolecta (DT) increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) and improves diet-induced obesity (DIO) in C57BL/6J mice at thermoneutrality (30°C). Here, we investigated whether DT improves DIO in a thermoneutral UCP1-deficient (KO) animal. KO mice were fed a high-fat diet supplemented with DT for 12 weeks. Compared to control group without DT, body weight was significantly reduced in DT group with no difference in f...
Source: Bioscience, Biotechnology, and Biochemistry - October 1, 2023 Category: Biochemistry Authors: Yukari Yamashita Tamaki Takeuchi Yuki Endo Ayumi Goto Misa Uno Setsuko Sakaki Yuji Yamaguchi Hiroyuki Takenaka Hitoshi Yamashita Source Type: research