Bioscience, Biotechnology, and Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Anti-cancer effects of plant-derived Micromonospora sp. M2 against A549 and MCF-7 cell lines
Biosci Biotechnol Biochem. 2024 Apr 4:zbae036. doi: 10.1093/bbb/zbae036. Online ahead of print.ABSTRACTThe huge diversity of secondary bioactive metabolites, such as antibiotic and anticancer compounds produced by Micromonospora sp., makes it an attractive target for study. Here, we explored the anti-proliferative activities of Micromonospora sp. M2 extract (MBE) in relation to its pro-oxidative activities in A549 and MCF7 cell lines. Anti-proliferative effects were assessed by treating cells with MBE. We found that treatment with MBE decreased cell proliferation and increased intracellular reactive oxygen species, and tha...
Source: Bioscience, Biotechnology, and Biochemistry - April 4, 2024 Category: Biochemistry Authors: Gyeong Han Jeong Dong-Ho Bak Hanui Lee Ja Young Cho Seong Hee Kang Byung Yeoup Chung Sanghwa Park Hyoung-Woo Bai Source Type: research

Solubilization and stabilization of lipoic acid trisulfide by creation of various β-cyclodextrin clathrates
Biosci Biotechnol Biochem. 2024 Apr 3:zbae042. doi: 10.1093/bbb/zbae042. Online ahead of print.ABSTRACTLipoic acid trisulfide, a sulfane sulfur-containing trisulfide of α-lipoic acid, holds promise in pharmaceuticals, yet knowledge gaps persist regarding its synthesis, properties, and stability. Here, we synthesized the lipoic acid trisulfide with a purity exceeding 99% from α-lipoic acid on a gram scale and obtained novel β-cyclodextrin clathrates (84-95% yield). Differential scanning calorimetry confirmed the inclusion of lipoic acid trisulfide in β-cyclodextrins. The resulting β-cyclodextrin clathrates exhibited si...
Source: Bioscience, Biotechnology, and Biochemistry - April 3, 2024 Category: Biochemistry Authors: Shoichiro Tomonaga Hiroaki Ishimaru Takahiro Isobe Etsuo Ohshima Shinji Kitagaki Source Type: research

Catechol-O-methyltransferase and monoamine oxidase B inhibitory activities of Australian bee pollen
Biosci Biotechnol Biochem. 2024 Apr 1:zbae041. doi: 10.1093/bbb/zbae041. Online ahead of print.ABSTRACTBee pollen is an apicultural product collected by honeybees from flower stamens and used as a functional food worldwide. In the present study, we aimed to elucidate the functions of Australian bee pollen. Australian bee pollen extracts and their main components were tested for catechol-O-methyltransferase (COMT) and monoamine oxidase B (MAOB) inhibitory activities. These enzymes are key neurotransmitters involved in Parkinson's disease and depression. Myricetin (5), tricetin (6), and luteolin (7) exhibited high COMT inhib...
Source: Bioscience, Biotechnology, and Biochemistry - April 1, 2024 Category: Biochemistry Authors: Chie Watanabe Aoi Yanagihara Ryo Miyata Taichi Mitsui Chihiro Honda Daisuke Fujinami Shigenori Kumazawa Source Type: research

Chemical modification of Arthrobacter sarcosine oxidase by N-methylisothiazolinone reduces reactivity toward oxygen
In this study, SoxA was chemically modified using MIT (mo-SoxA), and its structural and chemical properties were characterised. Spectral analysis data, oxygen consumption rates, and reactions were compared between intact and mo-SoxA. These demonstrate that the oxidative half-reaction toward oxygen is inhibited by MIT modification. The oxidase activity of mo-SoxA was approximately 2.1% of that of intact SoxA, and its dehydrogenase activity was approximately 4.2 times higher. The C-to-S mutants revealed that cooperative modification of two specific cysteine residues caused a drastic change in the enzyme reaction mode. Based ...
Source: Bioscience, Biotechnology, and Biochemistry - March 30, 2024 Category: Biochemistry Authors: Fuka Toyama Hironori Kimura Yuqi Zhang Yoshiaki Nishiya Source Type: research

Structure-activity relationship of anti-inflammatory meroterpenoids isolated from Dictyopteris polypodioides in RAW264 cells
In this study, we explored anti-inflammatory compounds from the brown alga Dictyopteris polypodioides and isolated 7 meroterpenoids. Their anti-inflammatory activities were evaluated using the lipopolysaccharide (LPS)-stimulated mouse macrophage cell line, RAW264. Yahazunol (1) exhibited similar nitric oxide (NO) production inhibitory activity as zonarol (2), which has previously been shown to be an anti-inflammatory compound. Yahazunol (1), zonarol (2), and isozonarol (3) inhibited not only nitric oxide production but also inducible nitric oxide synthase (iNOS), interleukin-6 (IL6), and C-C motif chemokine ligand 2 (CCL2)...
Source: Bioscience, Biotechnology, and Biochemistry - March 30, 2024 Category: Biochemistry Authors: Momochika Kumagai Akana Matsuda Nozomi Shiiba Tomoki Tsuruta Hikaru Endo Keisuke Nishikawa Yoshiki Morimoto Source Type: research

Reclassification of a reveromycin-producer and the proposals of Actinacidiphila reveromycinica sp. nov. and Actinacidiphila acidipaludis comb. nov
Biosci Biotechnol Biochem. 2024 Mar 28:zbae032. doi: 10.1093/bbb/zbae032. Online ahead of print.ABSTRACTStreptomyces sp. SN-593, a reveromycin-producer, was previously thought to belong to the genus Streptomyces based on the morphological and chemotaxonomic characteristics. In this paper, we re-considered its taxonomic position according to the current criteria. Phylogenetic analysis using 16S rRNA gene sequences showed that the strain belongs to the genus Actinacidiphila. In multilocus sequence and phylogenomic analyses, the strain SN-593 represented a distinct evolutionary lineage within this genus and its closest neighb...
Source: Bioscience, Biotechnology, and Biochemistry - March 29, 2024 Category: Biochemistry Authors: Hisayuki Komaki Hiroshi Takagi Shunji Takahashi Hiroyuki Osada Source Type: research

Enzymatic extraction of anthocyanins from the pomace of aronia (Aronia melanocarpa)
This study demonstrated an effective enzymatic extraction method for application in the food industry.PMID:38544329 | DOI:10.1093/bbb/zbae037 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - March 28, 2024 Category: Biochemistry Authors: Jinman Kim Yejin Lee Hee-Jae Suh Hee-Seok Lee Chan Lee Source Type: research