The effect of Dunaliella tertiolecta supplementation on diet-induced obesity in UCP1-deficient mice

Biosci Biotechnol Biochem. 2023 Sep 30:zbad138. doi: 10.1093/bbb/zbad138. Online ahead of print.ABSTRACTWe previously demonstrated that dietary supplementation with Dunaliella tertiolecta (DT) increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) and improves diet-induced obesity (DIO) in C57BL/6J mice at thermoneutrality (30°C). Here, we investigated whether DT improves DIO in a thermoneutral UCP1-deficient (KO) animal. KO mice were fed a high-fat diet supplemented with DT for 12 weeks. Compared to control group without DT, body weight was significantly reduced in DT group with no difference in food intake. DT-supplemented mice exhibited lower adiposity and well-maintained multilocular morphology in BAT, in which a significant increase in gene expression of PR domain containing 16 was detected in DT group compared to control group. Moreover, increase in UCP2 level and/or decrease in ribosomal protein S6 phosphorylation were detected in adipose tissues of DT group relative to control group. These results suggest that DT supplementation improves DIO by stimulating UCP1-independent energy dissipation at thermoneutrality.PMID:37777845 | DOI:10.1093/bbb/zbad138

Source: Bioscience, Biotechnology, and Biochemistry - October 1, 2023 Category: Biochemistry Authors: Yukari Yamashita Tamaki Takeuchi Yuki Endo Ayumi Goto Misa Uno Setsuko Sakaki Yuji Yamaguchi Hiroyuki Takenaka Hitoshi Yamashita Source Type: research