Direct screening for effective shRNA with a single mismatch in human cells without laborious cloning

Biosci Biotechnol Biochem. 2023 Oct 4:zbad143. doi: 10.1093/bbb/zbad143. Online ahead of print.ABSTRACTWe have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.PMID:37793878 | DOI:10.1093/bbb/zbad143
Source: Bioscience, Biotechnology, and Biochemistry - Category: Biochemistry Authors: Source Type: research