Degradation of DDT by γ-HCH dehydrochlorinase LinA

In this study, we found that γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase LinA from a γ-HCH-degrading bacterium, Sphingobium japonicum UT26, converts DDT to 1,1-dichloro-2,2-bis(4-chlorophenyl)-ethylene (DDE). Because of the weak DDT degradation activity of LinA, we could not detect such activity in UT26 cells expressing LinA constitutively. However, the linA-deletion mutant of UT26 harboring a plasmid for the expression of LinA, in which LinA was expressed at a higher level than UT26, showed the DDT degradation activity. This outcome highlights the potential for constructing DDT-degrading sphingomonad cells through elevated LinA expression.PMID:37796901 | DOI:10.1093/bbb/zbad141

Source: Bioscience, Biotechnology, and Biochemistry - October 5, 2023 Category: Biochemistry Authors: Kafayat Olaide Yusuf Habibullah Ren Ito Leonardo Stari Kouhei Kishida Yoshiyuki Ohtsubo Eiji Masai Masao Fukuda Keisuke Miyauchi Yuji Nagata Source Type: research