Bioscience, Biotechnology, and Biochemistry 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.A convenient fluorimetry-based degranulation assay using RBL-2H3 cells
In this study, we developed a simple fluorimetry-based degranulation assay using rat basophilic leukemia (RBL-2H3) mast cells. During degranulation, the styryl dye FM1-43 in the external solution fluorescently labeled the newly exocytosed secretory granules, whose increase in intensity was successively measured using a fluorescence microplate reader. In addition to the rate of β-hexosaminidase secretion, the cellular FM1-43 intensity successfully represented the degree and kinetics of degranulation under various conditions, suggesting that this method facilitates multi-sample and/or multi-time-point analyses required for ...
Source: Bioscience, Biotechnology, and Biochemistry - November 15, 2023 Category: Biochemistry Authors: Hironori Higashio Takuya Yokoyama Tomoyuki Saino Source Type: research
Eugeniin improves cholesterol metabolism in HepG2 cells and Caco-2 cells
This study sought, to elucidate the molecular mechanisms involved in this process using HepG2 and Caco-2 cells treated with 5 µM eugeniin. The intracellular cholesterol levels in HepG2 and Caco-2 cells were significantly decreased in 24 h eugeniin-treated group. The protein and mRNA levels of the low-density lipoprotein receptor (LDLR) were increased, while 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase protein and mRNA levels were decreased in HepG2 cells 6 h of eugeniin-treated group. Additionally, LDLR protein and mRNA levels were increased in HepG2 cells after 24 h of eugeniin treatment. In Caco-2, the protein and m...
Source: Bioscience, Biotechnology, and Biochemistry - November 12, 2023 Category: Biochemistry Authors: Yuyang Ye Asahi Takeuchi Yuya Kawaguchi Shoya Matsuba Ni Zhang Maihemuti Mijiti Arata Banno Naoto Hiramatsu Toshitaka Okada Satoshi Nagaoka Source Type: research
Eugeniin improves cholesterol metabolism in HepG2 cells and Caco-2 cells
This study sought, to elucidate the molecular mechanisms involved in this process using HepG2 and Caco-2 cells treated with 5 µM eugeniin. The intracellular cholesterol levels in HepG2 and Caco-2 cells were significantly decreased in 24 h eugeniin-treated group. The protein and mRNA levels of the low-density lipoprotein receptor (LDLR) were increased, while 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase protein and mRNA levels were decreased in HepG2 cells 6 h of eugeniin-treated group. Additionally, LDLR protein and mRNA levels were increased in HepG2 cells after 24 h of eugeniin treatment. In Caco-2, the protein and m...
Source: Bioscience, Biotechnology, and Biochemistry - November 12, 2023 Category: Biochemistry Authors: Yuyang Ye Asahi Takeuchi Yuya Kawaguchi Shoya Matsuba Ni Zhang Maihemuti Mijiti Arata Banno Naoto Hiramatsu Toshitaka Okada Satoshi Nagaoka Source Type: research
Eugeniin improves cholesterol metabolism in HepG2 cells and Caco-2 cells
This study sought, to elucidate the molecular mechanisms involved in this process using HepG2 and Caco-2 cells treated with 5 µM eugeniin. The intracellular cholesterol levels in HepG2 and Caco-2 cells were significantly decreased in 24 h eugeniin-treated group. The protein and mRNA levels of the low-density lipoprotein receptor (LDLR) were increased, while 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase protein and mRNA levels were decreased in HepG2 cells 6 h of eugeniin-treated group. Additionally, LDLR protein and mRNA levels were increased in HepG2 cells after 24 h of eugeniin treatment. In Caco-2, the protein and m...
Source: Bioscience, Biotechnology, and Biochemistry - November 12, 2023 Category: Biochemistry Authors: Yuyang Ye Asahi Takeuchi Yuya Kawaguchi Shoya Matsuba Ni Zhang Maihemuti Mijiti Arata Banno Naoto Hiramatsu Toshitaka Okada Satoshi Nagaoka Source Type: research
Eugeniin improves cholesterol metabolism in HepG2 cells and Caco-2 cells
This study sought, to elucidate the molecular mechanisms involved in this process using HepG2 and Caco-2 cells treated with 5 µM eugeniin. The intracellular cholesterol levels in HepG2 and Caco-2 cells were significantly decreased in 24 h eugeniin-treated group. The protein and mRNA levels of the low-density lipoprotein receptor (LDLR) were increased, while 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase protein and mRNA levels were decreased in HepG2 cells 6 h of eugeniin-treated group. Additionally, LDLR protein and mRNA levels were increased in HepG2 cells after 24 h of eugeniin treatment. In Caco-2, the protein and m...
Source: Bioscience, Biotechnology, and Biochemistry - November 12, 2023 Category: Biochemistry Authors: Yuyang Ye Asahi Takeuchi Yuya Kawaguchi Shoya Matsuba Ni Zhang Maihemuti Mijiti Arata Banno Naoto Hiramatsu Toshitaka Okada Satoshi Nagaoka Source Type: research
The nucleolar protein NIFK accelerates the progression of colorectal cancer via activating MYC pathway
This study aimed to explore the function of NIFK on colorectal cancer (CRC) and its associated molecular mechanisms. NIFK was upregulated in CRC tissues and cells. NIFK silencing resulted in reduced cell growth and metastasis, as well as in promoted apoptosis in CRC cells. Moreover, NIFK silencing was also confirmed to inhibit lipid accumulation and decrease fatty acid synthesis via downregulating lipogenic enzymes in CRC cells. Gene Set Enrichment Analysis and western blot co-verified that NIFK silencing inhibited MYC pathway in CRC cells. In addition, we also revealed that NIFK silencing function on cell growth, apoptosi...
Source: Bioscience, Biotechnology, and Biochemistry - November 11, 2023 Category: Biochemistry Authors: Tingting Xia Bin Wang Lingling Sun Source Type: research
Independent, cooperative regulation of cellulolytic genes by paralogous transcription factors ClbR and ClbR2 in Aspergillus aculeatus
Biosci Biotechnol Biochem. 2023 Nov 8:zbad156. doi: 10.1093/bbb/zbad156. Online ahead of print.ABSTRACTThe cellobiose-responsive regulator ClbR, a Zn(II)2Cys6 binuclear-cluster transcription factor, is a positive regulator of carbohydrate-active enzyme (CAZyme) genes responsive to cellulose in Aspergillus aculeatus. Because Zn(II)2Cys6 transcription factors tend to dimerize with proteins of the same family, we searched for a counterpart of ClbR and identified ClbR2, which is 42% identical to ClbR, as an interacting partner of ClbR by yeast two-hybrid screening. Genetic analyses suggested that ClbR and ClbR2 cooperatively r...
Source: Bioscience, Biotechnology, and Biochemistry - November 10, 2023 Category: Biochemistry Authors: Emi Kunitake Takashi Kawaguchi Shuji Tani Source Type: research
Mouse TMC4 is involved in the detection of chloride taste of salts
Biosci Biotechnol Biochem. 2023 Nov 8:zbad155. doi: 10.1093/bbb/zbad155. Online ahead of print.ABSTRACTLicking behavior with various salts in Tmc4 knockout (KO) mice was observed. In Tmc4 KO mice, a significant decrease in sensitivity to chloride salts, such as NaCl, KCl, and NH4Cl, was observed, while no significant decrease in sensitivity to Na gluconate was observed. This finding suggests that TMC4 may be involved in the detection of chloride taste.PMID:37947260 | DOI:10.1093/bbb/zbad155 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - November 10, 2023 Category: Biochemistry Authors: Masataka Narukawa Aya Masago Momo Murata Yoshikazu Saito Yoichi Kasahara Keiko Abe Tomiko Asakura Source Type: research
Independent, cooperative regulation of cellulolytic genes by paralogous transcription factors ClbR and ClbR2 in Aspergillus aculeatus
Biosci Biotechnol Biochem. 2023 Nov 8:zbad156. doi: 10.1093/bbb/zbad156. Online ahead of print.ABSTRACTThe cellobiose-responsive regulator ClbR, a Zn(II)2Cys6 binuclear-cluster transcription factor, is a positive regulator of carbohydrate-active enzyme (CAZyme) genes responsive to cellulose in Aspergillus aculeatus. Because Zn(II)2Cys6 transcription factors tend to dimerize with proteins of the same family, we searched for a counterpart of ClbR and identified ClbR2, which is 42% identical to ClbR, as an interacting partner of ClbR by yeast two-hybrid screening. Genetic analyses suggested that ClbR and ClbR2 cooperatively r...
Source: Bioscience, Biotechnology, and Biochemistry - November 10, 2023 Category: Biochemistry Authors: Emi Kunitake Takashi Kawaguchi Shuji Tani Source Type: research
Mouse TMC4 is involved in the detection of chloride taste of salts
Biosci Biotechnol Biochem. 2023 Nov 8:zbad155. doi: 10.1093/bbb/zbad155. Online ahead of print.ABSTRACTLicking behavior with various salts in Tmc4 knockout (KO) mice was observed. In Tmc4 KO mice, a significant decrease in sensitivity to chloride salts, such as NaCl, KCl, and NH4Cl, was observed, while no significant decrease in sensitivity to Na gluconate was observed. This finding suggests that TMC4 may be involved in the detection of chloride taste.PMID:37947260 | DOI:10.1093/bbb/zbad155 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - November 10, 2023 Category: Biochemistry Authors: Masataka Narukawa Aya Masago Momo Murata Yoshikazu Saito Yoichi Kasahara Keiko Abe Tomiko Asakura Source Type: research
Procyanidins and sensory nutrition; Do procyanidins modulate homeostasis via astringent taste receptors?
Biosci Biotechnol Biochem. 2023 Nov 1:zbad154. doi: 10.1093/bbb/zbad154. Online ahead of print.ABSTRACTLong-term intake of procyanidins has been suggested to reduce the risk of cardiovascular disease, dementia, and sensory function decline associated with aging. However, most of the ingested procyanidins are not absorbed and are excreted in the feces, so the mechanism of their beneficial impact is unknown. Procyanidins are the components of astringency in plant foods and their stimulation appears to be directly transmitted to the central nervous system via sensory nerves. Recent attention has been focused on the taste rece...
Source: Bioscience, Biotechnology, and Biochemistry - November 6, 2023 Category: Biochemistry Authors: Naomi Osakabe Taiki Fushimi Yasuyuki Fujii Vittorio Calabrese Source Type: research
Procyanidins and sensory nutrition; Do procyanidins modulate homeostasis via astringent taste receptors?
Biosci Biotechnol Biochem. 2023 Nov 1:zbad154. doi: 10.1093/bbb/zbad154. Online ahead of print.ABSTRACTLong-term intake of procyanidins has been suggested to reduce the risk of cardiovascular disease, dementia, and sensory function decline associated with aging. However, most of the ingested procyanidins are not absorbed and are excreted in the feces, so the mechanism of their beneficial impact is unknown. Procyanidins are the components of astringency in plant foods and their stimulation appears to be directly transmitted to the central nervous system via sensory nerves. Recent attention has been focused on the taste rece...
Source: Bioscience, Biotechnology, and Biochemistry - November 6, 2023 Category: Biochemistry Authors: Naomi Osakabe Taiki Fushimi Yasuyuki Fujii Vittorio Calabrese Source Type: research
Procyanidins and sensory nutrition; Do procyanidins modulate homeostasis via astringent taste receptors?
Biosci Biotechnol Biochem. 2023 Nov 1:zbad154. doi: 10.1093/bbb/zbad154. Online ahead of print.ABSTRACTLong-term intake of procyanidins has been suggested to reduce the risk of cardiovascular disease, dementia, and sensory function decline associated with aging. However, most of the ingested procyanidins are not absorbed and are excreted in the feces, so the mechanism of their beneficial impact is unknown. Procyanidins are the components of astringency in plant foods and their stimulation appears to be directly transmitted to the central nervous system via sensory nerves. Recent attention has been focused on the taste rece...
Source: Bioscience, Biotechnology, and Biochemistry - November 6, 2023 Category: Biochemistry Authors: Naomi Osakabe Taiki Fushimi Yasuyuki Fujii Vittorio Calabrese Source Type: research
Procyanidins and sensory nutrition; Do procyanidins modulate homeostasis via astringent taste receptors?
Biosci Biotechnol Biochem. 2023 Nov 1:zbad154. doi: 10.1093/bbb/zbad154. Online ahead of print.ABSTRACTLong-term intake of procyanidins has been suggested to reduce the risk of cardiovascular disease, dementia, and sensory function decline associated with aging. However, most of the ingested procyanidins are not absorbed and are excreted in the feces, so the mechanism of their beneficial impact is unknown. Procyanidins are the components of astringency in plant foods and their stimulation appears to be directly transmitted to the central nervous system via sensory nerves. Recent attention has been focused on the taste rece...
Source: Bioscience, Biotechnology, and Biochemistry - November 6, 2023 Category: Biochemistry Authors: Naomi Osakabe Taiki Fushimi Yasuyuki Fujii Vittorio Calabrese Source Type: research
α-Tocotrienol in rice bran enhances steroidogenesis in mouse Leydig cell via increased gene expression of steroidogenic acute regulatory protein and induction of its mitochondrial translocation
Biosci Biotechnol Biochem. 2023 Oct 25:zbad153. doi: 10.1093/bbb/zbad153. Online ahead of print.ABSTRACTRice is a staple food in the Asian region and one of the world's major energy sources. Testosterone is a steroid hormone that maintains physical, sexual, and cognitive ability and its decline causes health problems like late-onset hypogonadism. Evaluation of various grain extracts showed rice bran to stimulate testosterone secretion from Leydig model cell. α-Tocotrienol was found as a bioactive compound in rice bran, and mechanistic analysis showed the stimulation of steroid hormone synthesis through enhanced gene expre...
Source: Bioscience, Biotechnology, and Biochemistry - October 26, 2023 Category: Biochemistry Authors: Naofumi Terada Tomoaki Nagase Hiromi Kayoka Yusuke Adachi Eisuke Kato Source Type: research
