Bioprocess and Biosystems Engineering This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Catharanthus roseus-assisted bio-fabricated zinc oxide nanoparticles for promising antibacterial potential against Klebsiella pneumoniae
This study emphasized on the synthesis of zinc oxide nanoparticles (ZnO NPs) in an environmentally friendly manner from the extract of Catharanthus roseus leaves and its antibacterial assessment against the pneumonia-causing pathogen Klebsiella pneumoniae. This simple and convenient phytosynthesis approach is found to be beneficial over conventional methods, wherein plants serve as excellent reducing, capping, and stabilizing agents that enables the formation of ZnO NPs without the use of harmful chemicals. The formation of ZnO NPs was confirmed through several characterization techniques such as UV-visible spectroscopy, X...
Source: Bioprocess and Biosystems Engineering - March 25, 2024 Category: Biomedical Engineering Authors: Sumreen Sultana Bagepalli Shivaram Ashwini Mohammad Azam Ansari Mohammad N Alomary Yahya F Jamous Tekupalli Ravikiran Siddapura Ramachandrappa Niranjana M Yasmin Begum Ayesha Siddiqua Thimappa Ramachandrappa Lakshmeesha Source Type: research

Improved production of RNA-inhibiting antimicrobial peptide by Bacillus licheniformis MCC 2514 facilitated by a genetic algorithm optimized medium
In this study, a semi-synthetic medium was devised to overcome this challenge. Using a genetic algorithm, the production medium of AMP is optimized. The parent organism, Bacillus licheniformis MCC2514, produces AMP in very small quantities. This AMP is known to inhibit RNA biosynthesis. The findings revealed that lactose, NH4Cl and NaNO3 were crucial medium constituents for enhanced AMP synthesis. The potency of the AMP produced was studied using bacterium, Kocuria rhizophila ATCC 9341. The AMP produced from the optimized medium was eightfold higher than that produced from the unoptimized medium. Furthermore, activity was ...
Source: Bioprocess and Biosystems Engineering - March 24, 2024 Category: Biomedical Engineering Authors: Ishrat Jahan Peerzade Sarma Mutturi Prakash M Halami Source Type: research

Harnessing nature's potential: Alpinia galanga methanolic extract mediated green synthesis of silver nanoparticle, characterization and evaluation of anti-neoplastic activity
Bioprocess Biosyst Eng. 2024 Mar 21. doi: 10.1007/s00449-024-02993-7. Online ahead of print.ABSTRACTWith the advent of nanotechnology, the treatment of cancer is changing from a conventional to a nanoparticle-based approach. Thus, developing nanoparticles to treat cancer is an area of immense importance. We prepared silver nanoparticles (AgNPs) from methanolic extract of Alpinia galanga rhizome and characterized them by UV-Vis spectrophotometry, Fourier transform Infrared (FTIR) spectroscopy, Zetasizer, and Transmission electron Microscopy (TEM). UV-Vis spectrophotometry absorption spectrum showed surface plasmon between 4...
Source: Bioprocess and Biosystems Engineering - March 21, 2024 Category: Biomedical Engineering Authors: Ejaj Ahmad Alina Athar None Nimisha Qamar Zia Abhay Kumar Sharma Mohammed Sajid Mausumi Bharadwaj Mairaj Ahmed Ansari Sundeep Singh Saluja Source Type: research

Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications
This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa ...
Source: Bioprocess and Biosystems Engineering - March 21, 2024 Category: Biomedical Engineering Authors: Gopinath Venkatraman Priyadarshini Sakthi Mohan Puteri Shafinaz Abdul-Rahman Faridah Sonsudin Barathan Muttiah Abdurahman Hajinur Hirad Abdullah A Alarfaj Shifa Wang Source Type: research

Green synthesis of silver and gold nanoparticles in Callistemon viminalis extracts and their antimicrobial activities
Bioprocess Biosyst Eng. 2024 Mar 21. doi: 10.1007/s00449-024-02994-6. Online ahead of print.ABSTRACTIn the current study, the bottlebrush [Callistemon viminalis (Sol. ex Gaertn.) G. Don] plant was selected for the green synthesis of silver (Ag) and gold (Au) nanoparticles and to evaluate its antibacterial and antifungal activities. Phytochemical screening of C. viminalis confirmed the presence of alkaloids, anthraquinones, saponins, tannins, betacyanins, phlobatanins, coumarins, terpenoids, steroids, glycosides, and proteins. To characterize the synthesized Ag and Au NPs, UV-Visible spectroscopy, FTIR spectroscopy for func...
Source: Bioprocess and Biosystems Engineering - March 21, 2024 Category: Biomedical Engineering Authors: Shahid Khan Abdur Rauf Abdullah S M Aljohani Yahya S Al-Awthan Zubair Ahmad Omar S Bahattab Shehla Khan Muhammad Saadiq Shahid Ali Khan Rekha Thiruvengadam Muthu Thiruvengadam Source Type: research

Development strategy of non-GMO organism for increased hemoproteins in Corynebacterium glutamicum: a growth-acceleration-targeted evolution
Bioprocess Biosyst Eng. 2024 Mar 18. doi: 10.1007/s00449-024-02986-6. Online ahead of print.ABSTRACTHeme, found in hemoproteins, is a valuable source of iron, an essential mineral. The need for an alternative hemoprotein source has emerged due to the inherent risks of large-scale livestock farming and animal proteins. Corynebacterium glutamicum, regarded for Qualified Presumption of Safety or Generally Recognized as Safe, can biosynthesize hemoproteins. C. glutamicum single-cell protein (SCP) can be a valuable alternative hemoprotein for supplying heme iron without adversely affecting blood fat levels. We constructed the c...
Source: Bioprocess and Biosystems Engineering - March 19, 2024 Category: Biomedical Engineering Authors: Sehyeon Park Seungki Lee Taeyeon Kim Ahyoung Choi Soyeon Lee Pil Kim Source Type: research

Enhanced ε‑poly‑L‑lysine production in Streptomyces species by combining interspecific hybridization with multiple antibiotic resistance
Bioprocess Biosyst Eng. 2024 Mar 19. doi: 10.1007/s00449-024-02983-9. Online ahead of print.ABSTRACTTo improve the ε-PL production in wild-type strains of Streptomyces. albulus, Streptomyces. noursei, Streptomyces. rochei and Streptomyces. yunnanensis, the interspecific hybridization based on protoplast fusion was first performed. Two-species hybridizations failed to obtain hybrids with significant increase in ε-PL production, but four-species hybridizations succeed in acquiring many high-yield hybrids. 16S rDNA homology alignment and RAPD confirmed that the hybrid HX17 was restructured by integrating gene fragments from...
Source: Bioprocess and Biosystems Engineering - March 19, 2024 Category: Biomedical Engineering Authors: Shu Li Nan Wang Meichao Zhang Xiaoting Li Source Type: research