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Bio-fabrication of silver nanoparticles using Commelina erecta, L.: a mechanistic approach on synthesis, optimization, antibacterial, and antioxidant potential
This study reports, for the first time, the synthesis of silver nanoparticles using antioxidant compounds present in C. erecta, L. plant extract. The antioxidant compounds identified through GC-MS belong to phenols, phenolic acids, and carboxylic acid groups. Furthermore, the exceptional antimicrobial and antioxidant properties exhibited by the synthesized silver nanoparticles offer new possibilities for their potential applications.PMID:38467928 | DOI:10.1007/s00449-024-02980-y (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - March 12, 2024 Category: Biomedical Engineering Authors: Swastika Mallick Nilotpala Pradhan Source Type: research

A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass
Bioprocess Biosyst Eng. 2024 Mar 12. doi: 10.1007/s00449-024-02988-4. Online ahead of print.ABSTRACTThe present study reports a highly thermostable β-glucosidase (GH3) from Rasamsonia emersonii that was heterologously expressed in Pichia pastoris. Extracellular β-glucosidase was purified to homogeneity using single step affinity chromatography with molecular weight of ~ 110 kDa. Intriguingly, the purified enzyme displayed high tolerance to inhibitors mainly acetic acid, formic acid, ferulic acid, vanillin and 5-hydroxymethyl furfural at concentrations exceeding those present in acid steam pretreated rice straw slurry use...
Source: Bioprocess and Biosystems Engineering - March 12, 2024 Category: Biomedical Engineering Authors: Yashika Raheja Varinder Singh Gaurav Sharma Adrian Tsang Bhupinder Singh Chadha Source Type: research

The function of microbial enzymes in breaking down soil contaminated with pesticides: a review
This article reviews the bioremediation of pesticide compounds in soil using microbial enzymes, including the enzymatic degradation pathway and the recent development of enzyme-mediated bioremediation. Enzyme-mediated bioremediation is divided into phase I and phase II, where the former increases the solubility of pesticide compounds through oxidation-reduction and hydrolysis reactions, while the latter transforms toxic pollutants into less toxic or nontoxic products through conjugation reactions. The identified enzymes that can degrade OC insecticides include dehalogenases, phenol hydroxylase, and laccases. Recent develop...
Source: Bioprocess and Biosystems Engineering - March 8, 2024 Category: Biomedical Engineering Authors: Xing Kai Chia Tony Hadibarata Risky Ayu Kristanti Muhammad Noor Hazwan Jusoh Inn Shi Tan Henry Chee Yew Foo Source Type: research

Thauera sp. for efficient nitrate removal in continuous denitrifying moving bed biofilm reactor
Bioprocess Biosyst Eng. 2024 Mar 5. doi: 10.1007/s00449-024-02977-7. Online ahead of print.ABSTRACTThauera is the most widely found dominant denitrifying genus in wastewater. In earlier study, MBBR augmented with a specially developed denitrifying five-membered bacterial consortium (DC5) where Thauera was found to be the most abundant and persistent genus. Therefore, to check the functional potential of Thauera in the removal of nitrate-containing wastewater in the present study Thauera sp.V14 one of the member of the consortium DC5 was used as the model organism. Thauera sp.V14 exhibited strong hydrophobicity, auto-aggreg...
Source: Bioprocess and Biosystems Engineering - March 5, 2024 Category: Biomedical Engineering Authors: Roshni J Patel Anuradha S Nerurkar Source Type: research

Development of a new sterilization method for microalgae media using calcium hypochlorite as the sterilant
Bioprocess Biosyst Eng. 2024 Mar 4. doi: 10.1007/s00449-024-02971-z. Online ahead of print.ABSTRACTCalcium hypochlorite (Ca(ClO)2), which can be stably stored in powder form for a long period, is widely used as a disinfectant in various fields. A new sterilization process was developed in the present study, where a microalgal medium was sterilized using 0.02% Ca(ClO)2, followed by complete neutralization of the Ca(ClO)2 within 8 h through catalytic reaction of an MnCl2-Na2EDTA complex and a synergistic effect of glucose. When comparing the growth of Chlorella vulgaris in the autoclaved medium, a 2.65 times greater maximum ...
Source: Bioprocess and Biosystems Engineering - March 4, 2024 Category: Biomedical Engineering Authors: Seong-Hun Jeong Woong Kim Jong-Hee Kwon Source Type: research

Extending the computational and experimental analysis of lipase active site selectivity
In conclusion, experimental results analysis shows higher enzymatic productivity in esterification reactions of 1294.83 μmol/h.mg, while the CRL and PPL demonstrated the lowest performance (189.87 μmol / h.mg and 23.96 μmol / h.mg, respectively). Thus, molecular docking and experimental results indicate that BCL is a more efficient lipase to produce fatty acids and esters from licuri oil with a high content of lauric acid. In addition, this study also demonstrates the application of molecular docking as an important tool for lipase screening to achieve more sustainable production of butyl esters with a view synthesis of...
Source: Bioprocess and Biosystems Engineering - March 4, 2024 Category: Biomedical Engineering Authors: C ésar A Rodrigues Jefferson C B Santos Milson S Barbosa Milena C Lisboa Ranyere L Souza Adriano A Mendes Matheus M Pereira Álvaro S Lima Cleide M F Soares Source Type: research

Acceleration of the biodegradation of cationic polyacrylamide by the coupling effect of thermophilic microorganisms and high temperature in hyperthermophilic composting
In this study, the effects of HTC and conventional thermophilic composting (CTC) on CPAM in sludge were compared and analyzed. At the end of HTC and CTC, the concentrations of CPAM were 278.96 mg kg-1 and 533.89 mg kg-1, respectively, and the removal rates were 72.17% and 46.61%, respectively. The coupling effect of thermophilic microorganisms and high temperature improved the efficiency of HTC and accelerated the biodegradation of CPAM. The diversity and composition of microbial community changed dramatically during HTC. Geobacillus, Thermobispora, Pseudomonas, Brevundimonas, and Bacillus were the dominant bacteria respon...
Source: Bioprocess and Biosystems Engineering - February 29, 2024 Category: Biomedical Engineering Authors: Tianwen Song Fan Zhang Qu Chen Yinglu Tao Wei Chang Wenxiang Xia Wande Ding Jiafeng Jin Source Type: research

Formulation of silver nanoparticles using Duabanga grandiflora leaf extract and evaluation of their versatile therapeutic applications
Bioprocess Biosyst Eng. 2024 Feb 29. doi: 10.1007/s00449-024-02975-9. Online ahead of print.ABSTRACTThe current research focused on the green synthesis of silver nanoparticles (AgNPs) using Duabanga grandiflora leaf extract. The green synthesis of AgNPs was confirmed by the surface plasmon resonance band at 453 nm in a UV-Visible analysis. The formulated AgNPs had a diameter of around 99.72 nm with a spherical shape. Fourier transform infrared (FTIR) spectrum revealed the bio-reducing potential of phytochemicals present in D. grandiflora, which fundamentally influenced the synthesis of AgNPs. Zeta potential, dynamic light ...
Source: Bioprocess and Biosystems Engineering - February 29, 2024 Category: Biomedical Engineering Authors: Priya Das Gouhar Jahan Ashraf Tania Baishya Tarun Kumar Dua Paramita Paul Gouranga Nandi Ankita Dutta Divya Limbu Anoop Kumar Manab Deb Adhikari Saikat Dewanjee Ranabir Sahu Source Type: research

Handling the inhibitory role of product accumulation during sophorolipid fermentation in a bubble column reactor with an in situ foam recovery
Bioprocess Biosyst Eng. 2024 Feb 29. doi: 10.1007/s00449-024-02970-0. Online ahead of print.ABSTRACTSophorolipid (SL) production by Candida catenulata from sunflower fatty acids was studied in a bubble column reactor (BCR). The specific oxygen uptake rate was 0.021 mg gcell-1 min-1 which indicates the importance of aeration in SL biosynthesis. The measurement of oxygen transfer rate (OTR) in the BCR showed a satisfactory OTR value of about 0.093 min-1 in the system. However, further SL production was stopped after 30 h in the BCR mainly due to the product accumulation in the culture and its inhibitory effects on cell growt...
Source: Bioprocess and Biosystems Engineering - February 29, 2024 Category: Biomedical Engineering Authors: Fariba Amiri Alireza Habibi Source Type: research