Bioprocess and Biosystems Engineering This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Large-scale crystallization as an intermediate processing step in insulin downstream process: explored advantages and identified tool for process intensification
Bioprocess Biosyst Eng. 2023 Nov 8. doi: 10.1007/s00449-023-02931-z. Online ahead of print.ABSTRACTThe rising global prevalence of diabetes and increasing demand for insulin, calls for an increase in accessibility and affordability of insulin drugs through efficient and cost-effective manufacturing processes. Often downstream operations become manufacturing bottlenecks while processing a high volume of product. Thus, process integration and intensification play an important role in reducing process steps and time, volume reduction, and lower equipment footprints, which brings additional process efficiencies and lowers the ...
Source: Bioprocess and Biosystems Engineering - November 8, 2023 Category: Biomedical Engineering Authors: Partha Hazra Madhavan Buddha Chinnappa Reddy Indranil Gupta Source Type: research

Effect of blocking the haem synthesis pathway and weakening the haem synthesis pathway for sirohaem on the growth of and vitamin B < sub > 12 < /sub > synthesis in Ensifer adhaerens Casida A
Bioprocess Biosyst Eng. 2023 Nov 6. doi: 10.1007/s00449-023-02939-5. Online ahead of print.ABSTRACTTo block and weaken the bacterial branched VB12 synthetic metabolic pathway, homologous recombination technology was used to knock out the sirohaem synthase gene cysG located in the chromosome and the endogenous A plasmid of the Ensifer adhaerens Casida A strain, and the expression of the uroporphyrinogen III decarboxylase gene hemE was weakened by weak promoter substitution. The growth of the engineered strains and the production of VB12 and haem were analysed and measured in the engineered strains, aiming to provide a new s...
Source: Bioprocess and Biosystems Engineering - November 6, 2023 Category: Biomedical Engineering Authors: Yongheng Liu Yongyong Chang Qi Wang Wei Huang Cilang Ma Jianyu Su Source Type: research

Model construction and theoretical evaluation of the performance improvement of acetone-butanol-ethanol extractive fermentation by adding surfactant
Bioprocess Biosyst Eng. 2023 Nov 4. doi: 10.1007/s00449-023-02942-w. Online ahead of print.ABSTRACTSevere butanol toxicity to the metabolism of solventogenic clostridia significantly impede the application of fermentative butanol as a biofuel. Liquid-liquid extraction is an efficient method to reduce the butanol toxicity by in-situ removing it in the extractant phase. Butanol mass transfer into extractant phase in static acetone-butanol-ethanol (ABE) extractive fermentation with biodiesel as the extractant could be enhanced by adding a tiny amount of surfactant such as tween-80. In the case of corn-based ABE extractive fer...
Source: Bioprocess and Biosystems Engineering - November 4, 2023 Category: Biomedical Engineering Authors: Shijie Wang Jiabin Wang Zheng Gui Lina Liu Shuo Xu Yufen Guo Tairan Zhou Jin Cao Ruihong Gao Fang Xie Aiyong He Hongzhen Luo Source Type: research

Chicken viscera meal as substrate for the simultaneous production of antioxidant compounds and proteases by Aspergillus oryzae
Bioprocess Biosyst Eng. 2023 Nov 2. doi: 10.1007/s00449-023-02934-w. Online ahead of print.ABSTRACTThe use of chicken waste can contribute to the development of new processes and obtaining molecules with high added value. An experimental design was applied to evaluate the effect of moisture, temperature, and inoculum size on the production of antioxidant peptides and proteases by A. oryzae IOC3999 through solid-state fermentation (SSF) of chicken viscera meal. As a result, the process conditions strongly influenced protease production and antioxidant activity of the fermented products. A global analysis of the results indi...
Source: Bioprocess and Biosystems Engineering - November 3, 2023 Category: Biomedical Engineering Authors: Yuri Matheus Silva Amaral Ruann Janser Soares de Castro Source Type: research

Implementation of cytochrome c proteins and carbon nanotubes hybrids in bioelectrodes towards bioelectrochemical systems applications
Bioprocess Biosyst Eng. 2023 Nov 3. doi: 10.1007/s00449-023-02933-x. Online ahead of print.ABSTRACTMultiheme cytochrome c (Cyt c) can function as a redox protein on electrode to accomplish bioelectrocatalysis. However, the direct electron transfer (DET) between the redox site of Cyt c and electrode is low due to the large coupling distance. A close proximity or a connection pathway from the deeply buried active site to the protein surface can be established by modifying the electrode with carbon nanotubes (CNTs) to improve the DET. Therefore, the isolated Cyt c has been assembled or casted with CNTs by various processes to...
Source: Bioprocess and Biosystems Engineering - November 3, 2023 Category: Biomedical Engineering Authors: Xinru Zhai Xiaojun Liu Huihui Dong Mingzhen Lin Xinxin Zheng Qinzheng Yang Source Type: research

Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources
This study investigates the effects of different strategies on poly(3-hydroxybutyrate)-P(3HB) production in a fed-batch bioreactor by Bacillus megaterium using candy industry effluent (CIE), sucrose, and rice parboiled water (RPW) as carbon sources. In biosynthesis, kinetic and stoichiometric parameters of substrate conversion into products and/or cells, productivity, instantaneous, and specific conversion rates were evaluated. The maximum concentration of P(3HB) was 4.00 g.L-1 (77% of the total dry mass) in 42 h of cultivation in minimal medium/RPW added with a carbon source based on CIE, demonstrating that the fed-batch ...
Source: Bioprocess and Biosystems Engineering - October 26, 2023 Category: Biomedical Engineering Authors: Rafaela Nery De Melo Guilherme de Souza Hassemer do Lucas Henrique Nascimento Rosicler Colet Clarice Steffens Alexander Junges Eunice Valduga Source Type: research