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Automated Raman feed-back control of multiple supplemental feeds to enable an intensified high inoculation density fed-batch platform process
Bioprocess Biosyst Eng. 2023 Aug 26. doi: 10.1007/s00449-023-02912-2. Online ahead of print.ABSTRACTBiologics manufacturing is increasingly moving toward intensified processes that require novel control strategies in order to achieve higher titers in shorter periods of time compared to traditional fed-batch cultures. In order to implement these strategies for intensified processes, continuous process monitoring is often required. To this end, inline Raman spectroscopy was used to develop partial least squares models to monitor changes in residual concentrations of glucose, phenylalanine and methionine during the culture of...
Source: Bioprocess and Biosystems Engineering - August 26, 2023 Category: Biomedical Engineering Authors: Thaddaeus A Webster Brian C Hadley Marissa Dickson Jessica Hodgkins Mikayla Olin Nicolas Wolnick Julia Armstrong Carrie Mason Brandon Downey Source Type: research

Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-L-methionine
In this study, a genome-scale metabolic model (GEMs) was reconstructed and used to assess P. pastoris' metabolic capabilities for the production of S-adenosyl-L-methionine (AdoMet or SAM or SAMe) from individual carbon sources along with the addition of L-methionine. In a model-driven P. pastoris strain, the well-established genome-scale metabolic model iAUKM can be implemented to predict high valuable metabolite production. The model, iAUKM, was created by merging the previously published iMT1026 model and the draught model generated using Raven toolbox from the KEGG database which covered 2309 enzymatic reactions associa...
Source: Bioprocess and Biosystems Engineering - August 19, 2023 Category: Biomedical Engineering Authors: Kabilan Subash Chandra Bose Mohd Imran Shah Jayachandran Krishna Meenakshisundaram Sankaranarayanan Source Type: research

Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-L-methionine
In this study, a genome-scale metabolic model (GEMs) was reconstructed and used to assess P. pastoris' metabolic capabilities for the production of S-adenosyl-L-methionine (AdoMet or SAM or SAMe) from individual carbon sources along with the addition of L-methionine. In a model-driven P. pastoris strain, the well-established genome-scale metabolic model iAUKM can be implemented to predict high valuable metabolite production. The model, iAUKM, was created by merging the previously published iMT1026 model and the draught model generated using Raven toolbox from the KEGG database which covered 2309 enzymatic reactions associa...
Source: Bioprocess and Biosystems Engineering - August 19, 2023 Category: Biomedical Engineering Authors: Kabilan Subash Chandra Bose Mohd Imran Shah Jayachandran Krishna Meenakshisundaram Sankaranarayanan Source Type: research

Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-L-methionine
In this study, a genome-scale metabolic model (GEMs) was reconstructed and used to assess P. pastoris' metabolic capabilities for the production of S-adenosyl-L-methionine (AdoMet or SAM or SAMe) from individual carbon sources along with the addition of L-methionine. In a model-driven P. pastoris strain, the well-established genome-scale metabolic model iAUKM can be implemented to predict high valuable metabolite production. The model, iAUKM, was created by merging the previously published iMT1026 model and the draught model generated using Raven toolbox from the KEGG database which covered 2309 enzymatic reactions associa...
Source: Bioprocess and Biosystems Engineering - August 19, 2023 Category: Biomedical Engineering Authors: Kabilan Subash Chandra Bose Mohd Imran Shah Jayachandran Krishna Meenakshisundaram Sankaranarayanan Source Type: research

Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-L-methionine
In this study, a genome-scale metabolic model (GEMs) was reconstructed and used to assess P. pastoris' metabolic capabilities for the production of S-adenosyl-L-methionine (AdoMet or SAM or SAMe) from individual carbon sources along with the addition of L-methionine. In a model-driven P. pastoris strain, the well-established genome-scale metabolic model iAUKM can be implemented to predict high valuable metabolite production. The model, iAUKM, was created by merging the previously published iMT1026 model and the draught model generated using Raven toolbox from the KEGG database which covered 2309 enzymatic reactions associa...
Source: Bioprocess and Biosystems Engineering - August 19, 2023 Category: Biomedical Engineering Authors: Kabilan Subash Chandra Bose Mohd Imran Shah Jayachandran Krishna Meenakshisundaram Sankaranarayanan Source Type: research