Comparative analysis of the effect of hypoxia in two different tumor cell models shows the differential involvement of PTEN control of proangiogenic pathways
Biochem Cell Biol. 2023 Jul 17. doi: 10.1139/bcb-2023-0047. Online ahead of print.ABSTRACTHypoxia, low, non-physiological oxygen tension is a key regulator of tumor microenvironment, determining the pathological tumor vascularization. Alleviation of hypoxia through vessel normalization may be a promising therapeutic approach. We aimed to assess the role of low oxygen tension in PTEN related pathways and proangiogenic response, in vitro, in two different tumor cell lines, focusing on potential therapeutic targets for tumor vessels normalization. Down-regulation of PTEN in hypoxia mediates the activation of distinct mechanis...
Source: Biochemistry and Cell Biology - July 17, 2023 Category: Biochemistry Authors: Aleksandra Majewska Klaudia Brodaczewska Aleksandra Filipiak-Duliban Clauidne Kieda Source Type: research
Comparative analysis of the effect of hypoxia in two different tumor cell models shows the differential involvement of PTEN control of proangiogenic pathways
Biochem Cell Biol. 2023 Jul 17. doi: 10.1139/bcb-2023-0047. Online ahead of print.ABSTRACTHypoxia, low, non-physiological oxygen tension is a key regulator of tumor microenvironment, determining the pathological tumor vascularization. Alleviation of hypoxia through vessel normalization may be a promising therapeutic approach. We aimed to assess the role of low oxygen tension in PTEN related pathways and proangiogenic response, in vitro, in two different tumor cell lines, focusing on potential therapeutic targets for tumor vessels normalization. Down-regulation of PTEN in hypoxia mediates the activation of distinct mechanis...
Source: Biochemistry and Cell Biology - July 17, 2023 Category: Biochemistry Authors: Aleksandra Majewska Klaudia Brodaczewska Aleksandra Filipiak-Duliban Clauidne Kieda Source Type: research
The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope
In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer ...
Source: Biochemistry and Cell Biology - July 12, 2023 Category: Biochemistry Authors: Iraj Rezaei Ali Sadeghi Source Type: research
The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope
In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer ...
Source: Biochemistry and Cell Biology - July 12, 2023 Category: Biochemistry Authors: Iraj Rezaei Ali Sadeghi Source Type: research
The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope
In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer ...
Source: Biochemistry and Cell Biology - July 12, 2023 Category: Biochemistry Authors: Iraj Rezaei Ali Sadeghi Source Type: research
The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope
In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer ...
Source: Biochemistry and Cell Biology - July 12, 2023 Category: Biochemistry Authors: Iraj Rezaei Ali Sadeghi Source Type: research
The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope
In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer ...
Source: Biochemistry and Cell Biology - July 12, 2023 Category: Biochemistry Authors: Iraj Rezaei Ali Sadeghi Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil
In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison to normal colorectal epithelial cells. Depletion of PTGES significantly enhanced the inhibitory effect of 5FU on CRC cell viability that was fully reverted by exogenous supplement of PGE2. Inhibition of PTGES enzymatic function, by either inducing loss-of-function mutant or treatment with selective inhibitors, phenocopied the PTGES depletion in terms of 5FU sensitization. Mechanistical...
Source: Biochemistry and Cell Biology - June 26, 2023 Category: Biochemistry Authors: Song Geng Hao Zhan Lianmeng Cao Longlong Geng Xiang Ren Source Type: research
