Histone H4 asymmetrically dimethylated at arginine 3 (H4R3me2a), a mark of super-enhancers
In this report, we analyzed chromatin immunoprecipitation sequencing data for the genomic location of H4R3me2a in the breast cancer cell line MCF7. As in avian cells, MCF7 H4R3me2a is present in intronic and intergenic regions. Nucleosomes with H4R3me2a and H3K27ac next to nucleosome-free regions are found at super-enhancers, enhancers, and promoter regions of expressed genes. Genes with critical roles in breast cancer cells have broad domains of nucleosomes with H4R3me2a, H3K27ac, and H3K4me3. Our results are consistent with PRMT1-mediated H4R3me2a playing a key role in the chromatin organization of regulatory regions of ...
Source: Biochemistry and Cell Biology - November 27, 2023 Category: Biochemistry Authors: Sadhana R N Sudhakar Li Wu Shrinal Patel Athanasios Zovoilis James R Davie Source Type: research
Histone H4 asymmetrically dimethylated at arginine 3 (H4R3me2a), a mark of super-enhancers
In this report, we analyzed chromatin immunoprecipitation sequencing data for the genomic location of H4R3me2a in the breast cancer cell line MCF7. As in avian cells, MCF7 H4R3me2a is present in intronic and intergenic regions. Nucleosomes with H4R3me2a and H3K27ac next to nucleosome-free regions are found at super-enhancers, enhancers, and promoter regions of expressed genes. Genes with critical roles in breast cancer cells have broad domains of nucleosomes with H4R3me2a, H3K27ac, and H3K4me3. Our results are consistent with PRMT1-mediated H4R3me2a playing a key role in the chromatin organization of regulatory regions of ...
Source: Biochemistry and Cell Biology - November 27, 2023 Category: Biochemistry Authors: Sadhana R N Sudhakar Li Wu Shrinal Patel Athanasios Zovoilis James R Davie Source Type: research
Correction: Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells
Biochem Cell Biol. 2023 Nov 24. doi: 10.1139/bcb-2023-0321. Online ahead of print.NO ABSTRACTPMID:37997895 | DOI:10.1139/bcb-2023-0321 (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 24, 2023 Category: Biochemistry Authors: Hongbo Luo Wei Sun Jin Shao Huiping Ma Zhengping Jia Linlin Jing Source Type: research
Correction: Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells
Biochem Cell Biol. 2023 Nov 24. doi: 10.1139/bcb-2023-0321. Online ahead of print.NO ABSTRACTPMID:37997895 | DOI:10.1139/bcb-2023-0321 (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 24, 2023 Category: Biochemistry Authors: Hongbo Luo Wei Sun Jin Shao Huiping Ma Zhengping Jia Linlin Jing Source Type: research
Correction: Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells
Biochem Cell Biol. 2023 Nov 24. doi: 10.1139/bcb-2023-0321. Online ahead of print.NO ABSTRACTPMID:37997895 | DOI:10.1139/bcb-2023-0321 (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 24, 2023 Category: Biochemistry Authors: Hongbo Luo Wei Sun Jin Shao Huiping Ma Zhengping Jia Linlin Jing Source Type: research
The role of BCL2L13 in Glioblastoma: Turning a need into a target
Biochem Cell Biol. 2023 Nov 21. doi: 10.1139/bcb-2023-0221. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most common aggressive central nervous system (CNS) cancer. GBM has a high mortality rate, with a median survival time of 12-15 months after diagnosis. A poor prognosis and a shorter life expectancy may result from resistance to standard treatments such as radiation and chemotherapy. Temozolomide (TMZ) has been the mainstay treatment for GBM, but unfortunately, there are high rates of resistance with GBM bypassing apoptosis. A proposed mechanism for bypassing apoptosis is decreased ceramide levels, and previo...
Source: Biochemistry and Cell Biology - November 21, 2023 Category: Biochemistry Authors: Joadi Jacobs Rosa Iranpour Amir Barzegar Behrooz Simone C da Silva Rosa Saeid Ghavami Source Type: research
The role of BCL2L13 in Glioblastoma: Turning a need into a target
Biochem Cell Biol. 2023 Nov 21. doi: 10.1139/bcb-2023-0221. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most common aggressive central nervous system (CNS) cancer. GBM has a high mortality rate, with a median survival time of 12-15 months after diagnosis. A poor prognosis and a shorter life expectancy may result from resistance to standard treatments such as radiation and chemotherapy. Temozolomide (TMZ) has been the mainstay treatment for GBM, but unfortunately, there are high rates of resistance with GBM bypassing apoptosis. A proposed mechanism for bypassing apoptosis is decreased ceramide levels, and previo...
Source: Biochemistry and Cell Biology - November 21, 2023 Category: Biochemistry Authors: Joadi Jacobs Rosa Iranpour Amir Barzegar Behrooz Simone C da Silva Rosa Saeid Ghavami Source Type: research
The role of BCL2L13 in Glioblastoma: Turning a need into a target
Biochem Cell Biol. 2023 Nov 21. doi: 10.1139/bcb-2023-0221. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most common aggressive central nervous system (CNS) cancer. GBM has a high mortality rate, with a median survival time of 12-15 months after diagnosis. A poor prognosis and a shorter life expectancy may result from resistance to standard treatments such as radiation and chemotherapy. Temozolomide (TMZ) has been the mainstay treatment for GBM, but unfortunately, there are high rates of resistance with GBM bypassing apoptosis. A proposed mechanism for bypassing apoptosis is decreased ceramide levels, and previo...
Source: Biochemistry and Cell Biology - November 21, 2023 Category: Biochemistry Authors: Joadi Jacobs Rosa Iranpour Amir Barzegar Behrooz Simone C da Silva Rosa Saeid Ghavami Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis
This study critically investigated effects of various lysis conditions on sperm RNA isolation. Sperm suspended in TRIzol were subjected to a combination of mechanical treatments (sonication and passage through a 30G needle and syringe) and chemical treatments (supplementation with reducing agents DTT (1,4-dithiothreitol) and TCEP [Tris(2-carboxyethyl) phosphine hydrochloride)]. Microscopic evaluation of sperm lysis confirmed preferential sperm tail versus sperm head lysis. Interestingly, only TCEP-supplemented TRIzol (both mechanical treatments) had progressive sperm head lysis and consistently yielded total-sperm RNA. Fur...
Source: Biochemistry and Cell Biology - November 10, 2023 Category: Biochemistry Authors: Saurabh Tiwari Abdallah Shahat John Kastelic Nehal Thakor Jacob Thundathil Source Type: research
