The sperm nuclear basic proteins of the sword fern (Polystichum munitum)
Biochem Cell Biol. 2024 Feb 12. doi: 10.1139/bcb-2023-0343. Online ahead of print.ABSTRACTSperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants. Histones pre...
Source: Biochemistry and Cell Biology - February 12, 2024 Category: Biochemistry Authors: Juan Ausio Alistair Knox Bo-Hyun Kim Elaine Humphrey Brent Gowen Naoki Minamino Patrick von Aderkas Source Type: research
The sperm nuclear basic proteins of the sword fern (Polystichum munitum)
Biochem Cell Biol. 2024 Feb 12. doi: 10.1139/bcb-2023-0343. Online ahead of print.ABSTRACTSperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants. Histones pre...
Source: Biochemistry and Cell Biology - February 12, 2024 Category: Biochemistry Authors: Juan Ausio Alistair Knox Bo-Hyun Kim Elaine Humphrey Brent Gowen Naoki Minamino Patrick von Aderkas Source Type: research
The sperm nuclear basic proteins of the sword fern (Polystichum munitum)
Biochem Cell Biol. 2024 Feb 12. doi: 10.1139/bcb-2023-0343. Online ahead of print.ABSTRACTSperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants. Histones pre...
Source: Biochemistry and Cell Biology - February 12, 2024 Category: Biochemistry Authors: Juan Ausio Alistair Knox Bo-Hyun Kim Elaine Humphrey Brent Gowen Naoki Minamino Patrick von Aderkas Source Type: research
The sperm nuclear basic proteins of the sword fern (Polystichum munitum)
Biochem Cell Biol. 2024 Feb 12. doi: 10.1139/bcb-2023-0343. Online ahead of print.ABSTRACTSperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants. Histones pre...
Source: Biochemistry and Cell Biology - February 12, 2024 Category: Biochemistry Authors: Juan Ausio Alistair Knox Bo-Hyun Kim Elaine Humphrey Brent Gowen Naoki Minamino Patrick von Aderkas Source Type: research
The sperm nuclear basic proteins of the sword fern (Polystichum munitum)
Biochem Cell Biol. 2024 Feb 12. doi: 10.1139/bcb-2023-0343. Online ahead of print.ABSTRACTSperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants. Histones pre...
Source: Biochemistry and Cell Biology - February 12, 2024 Category: Biochemistry Authors: Juan Ausio Alistair Knox Bo-Hyun Kim Elaine Humphrey Brent Gowen Naoki Minamino Patrick von Aderkas Source Type: research
ChestCovidNet: An Effective DL-based Approach for COVID-19, Lung Opacity, and Pneumonia Detection Using Chest Radiographs Images
This study proposes a trustworthy, robust, and lightweight network (ChestCovidNet) that can detect COVID-19 by examining various CRIs datasets. The ChestCovidNet model has only 11 learned layers, eight convolutional (Conv) layers, and three fully connected (FC) layers. The framework employs both the Conv and group Conv layers, Leaky Relu activation function, shufflenet unit, Conv kernels of 3×3 and 1×1 to extract features at different scales, and two normalization procedures that are cross-channel normalization and batch normalization. We used 9013 CRIs for training whereas 3863 CRIs for testing the proposed ChestCovidNe...
Source: Biochemistry and Cell Biology - February 2, 2024 Category: Biochemistry Authors: Naeem Ullah Javed Ali Khan Sultan Almakdi Mohammed S Alshehri Mimonah Al Qathrady Muhammad Shahid Anwar Ikram Syed Source Type: research
Note of appreciation
Biochem Cell Biol. 2024 Feb 1;102(1):i. doi: 10.1139/bcb-2023-0272.NO ABSTRACTPMID:38299505 | DOI:10.1139/bcb-2023-0272 (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - February 1, 2024 Category: Biochemistry Source Type: research
Decreased NMIIA heavy chain phosphorylation at S1943 promotes mitoxantrone resistance by upregulating BCRP and N-cadherin expression in breast cancer cells
In this study, we revealed that the expression of NMIIA heavy chain phosphorylation at S1943 was downregulated in BCRP-overexpressing breast cancer MCF-7/MX cells, and stable expression of NMIIA-S1943A mutant increased BCRP expression and promoted the resistance of MCF-7/MX cells to MX. Meanwhile, NMIIA S1943 phosphorylation induced by epidermal growth factor (EGF) was accompanied by the downregulation of BCRP in MCF-7/MX cells. Furthermore, stable expression of NMIIA-S1943A in MCF-7/MX cells resulted in upregulation of N-cadherin and the accumulation of β-catenin on the cell surface, which inhibited the nucleus transloca...
Source: Biochemistry and Cell Biology - January 8, 2024 Category: Biochemistry Authors: Kemin Li Tian Li Yanan Niu Yu Gao Yifan Shi Yifan He Xuanping Zhang Yan Wang Jing Cao Xiaoling Hu Min Chen Ruizan Shi Source Type: research
Decreased NMIIA heavy chain phosphorylation at S1943 promotes mitoxantrone resistance by upregulating BCRP and N-cadherin expression in breast cancer cells
In this study, we revealed that the expression of NMIIA heavy chain phosphorylation at S1943 was downregulated in BCRP-overexpressing breast cancer MCF-7/MX cells, and stable expression of NMIIA-S1943A mutant increased BCRP expression and promoted the resistance of MCF-7/MX cells to MX. Meanwhile, NMIIA S1943 phosphorylation induced by epidermal growth factor (EGF) was accompanied by the downregulation of BCRP in MCF-7/MX cells. Furthermore, stable expression of NMIIA-S1943A in MCF-7/MX cells resulted in upregulation of N-cadherin and the accumulation of β-catenin on the cell surface, which inhibited the nucleus transloca...
Source: Biochemistry and Cell Biology - January 8, 2024 Category: Biochemistry Authors: Kemin Li Tian Li Yanan Niu Yu Gao Yifan Shi Yifan He Xuanping Zhang Yan Wang Jing Cao Xiaoling Hu Min Chen Zan Rui Shi Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
Evolution of techniques and tools for replication fork proteome and protein interaction studies
This article provides an overview of MS techniques and their applications in proteomics studies, with a focus on the replication fork proteome. The replication fork is a multi-protein assembly involved in DNA replication, and its proper functioning is crucial for maintaining genomic integrity. By combining quantitative MS labeling techniques with various data acquisition methods, researchers have made significant strides in elucidating the complex processes and molecular mechanisms at the replication fork. Overall, MS has revolutionized our understanding of protein dynamics, offering valuable insights into cellular process...
Source: Biochemistry and Cell Biology - December 19, 2023 Category: Biochemistry Authors: Carla-Marie Jurkovic Francois-Michel Boisvert Source Type: research
