The Effects of Cetuximab and Cisplatin Anti-Cancer Drugs on the Mechanical Properties of the Lung Cancerous Cells Using Atomic Force Microscope

In this study, the effect of Cetuximab and Cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope (AFM). The results show that Cetuximab increases the stiffness of A-549 cell from 1225 Pa to 3403 Pa and 12690 Pa for 24 and 48h incubations. The influence of Cetuximab on the of Calu-6 shows that the elastic modulus after 24 and 48h culture times increases about Cisplatin anti-cancer drug for A-549 cell indicates that the elastic modulus raises from 1225 Pa to 1506 Pa and 2375 Pa for 24 and 48h respectively. For Calu-6 cell, Cisplatin has an important role to increase the stiffness of the cell. Applying Cisplatin increases the elastic modulus from 33 Pa to 682.8 Pa for 24h and 1105 Pa after 48h incubations.PMID:37437307 | DOI:10.1139/bcb-2022-0322
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Source Type: research