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Temperature adaptation patterns in Chinese cattle revealed by TRPM2 gene mutation analysis
Anim Biotechnol. 2024 Jan 2:2299944. doi: 10.1080/10495398.2023.2299944. Online ahead of print.ABSTRACTCattle are sensitive to temperature fluctuations but adapt well to inclement weather conditions. When environmental temperatures exceed specific thresholds, heat stress becomes a critical concern for cattle. The TRPM2 gene, which resides on cattle chromosome 1 encodes a TRP channel protein, holding a unique capacity to sense temperature changes and facilitate rapid response to avoid heat stress. Here, we utilized the Bovine Genome Variation Database (BGVD) (http://animal.omics.pro/code/index.php/BosVar), and identified a ...
Source: Animal Biotechnology - January 2, 2024 Category: Biotechnology Authors: Dekai Liu Yifan Yang Zhefu Chen Yijie Fan Jianyong Liu Yibing Xu Zulfiqar Ahmed Jicai Zhang Fuqiang Li Xingshan Qi Weiru Song Kaixia Zhu Jiangcai Gongque Guomei Li Bizhi Huang Chuzhao Lei Source Type: research

Effect of DHCR7 on adipocyte differentiation in goats
Anim Biotechnol. 2023 Dec 29:2298399. doi: 10.1080/10495398.2023.2298399. Online ahead of print.ABSTRACTCholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remains unknown. Here, the effect of DHCR7 on the formation of subcutaneous and intramuscular fat in goats was investigated in vitro, and the result indicated that the mRNA level of DHCR7 showed a gradual downward trend in subcutane...
Source: Animal Biotechnology - December 29, 2023 Category: Biotechnology Authors: Zhibin Li Tingting Hu Ruiwen Li Jinlan Li Youli Wang Yanyan Li Yaqiu Lin Yong Wang Xing Jiani Source Type: research

Identification of AGO2 and PLEC genes polymorphisms in Hu sheep and their relationship with body size traits
This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 (AGO2) and Plectin (PLEC) genes in Hu sheep. Two intron mutations were found in Hu sheep, which were AGO2 g.51700 A > C and PLEC g.23157 C > T, respectively. Through association analysis of two mutation sites and body size traits, it was found that AGO2 g.51700 A > C mainly affects the chest and cannon circumference of Hu sheep of while PLEC g.23157 C mainly affects...
Source: Animal Biotechnology - December 27, 2023 Category: Biotechnology Authors: Jia Liu Wenxin Zheng Weimin Wang Xiaobin Yang Yongliang Huang Panpan Cui Zongwu Ma Xiwen Zeng Rui Zhai Xiuxiu Weng Weiwei Wu Xiaoxue Zhang Source Type: research

RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets
In this study, we analysed the ileum of Large White (LW) and Mashen (MS) piglets at different times of starvation using chemical staining and transcriptome analysis. The intestinal barrier of piglets was damaged after starvation stress, but the intestinal antistress ability of MS piglets was stronger than LW piglets. A total of 8021 differentially expressed genes (DEGs) were identified in two breeds. Interestingly, the immune capacity (CHUK, TLR3) of MS piglets increased significantly after short-term starvation stress, while energy metabolism (NAGS, PLA2G12B, AGCG8) was predominant in LW piglets. After long-term starvatio...
Source: Animal Biotechnology - December 26, 2023 Category: Biotechnology Authors: Yijia Ma Tong Guo Jianqin Ding Zhiling Dong Yifei Ren Chang Lu Yan Zhao Xiaohong Guo Guoqing Cao Bugao Li Pengfei Gao Source Type: research

Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide
Anim Biotechnol. 2023 Dec 23:1-12. doi: 10.1080/10495398.2023.2290527. Online ahead of print.ABSTRACTMastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by tra...
Source: Animal Biotechnology - December 23, 2023 Category: Biotechnology Authors: Jingjing Liu Yingkui Gao Xing Zhang Zhonghua Hao Huaqiang Zhang Rong Gui Fang Liu Chao Tong Xuebing Wang Source Type: research

Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat
Anim Biotechnol. 2023 Dec 23:1-10. doi: 10.1080/10495398.2023.2290528. Online ahead of print.ABSTRACTThe present study was designed with the aim to study morphometric characterization as well as phylogeny and diversity of the local Surguli goat at their breeding tract district Kohat through mitochondrial DNA region, i.e., Cytochrome C Oxidase Subunit One (CO1) gene. Morphometric data and blood samples were collected from thirty (30) pure goats. Morphometric analysis showed that sex had significant effect (p < 0.05) on body weight, body length, hearth girth and horn length while no significant effect (p > 0.05) was ob...
Source: Animal Biotechnology - December 23, 2023 Category: Biotechnology Authors: Muhammad Munir Khan Syed Muhammad Suhail Hafiz Abdul Majid Ijaz Ahmad Umer Sadique Rajwali Khan Iftikhar Ahmad Asim Ijaz Khalid Khan Farhad Ali Muhammad Saeed Khan Ahmed A El-Mansi Source Type: research